Cytosine-arabinoside-induced keratopathy: a model of corneal proliferation kinetics.

AIMS: The use of the chemotherapeutic cytosine arabinoside (Ara-C) causes an ocular toxic reaction, characterized by aspecific keratopathy. We examined the pathway of the damaged cells by in vivo confocal microscopy. METHODS: Prospective study of 11 patients with acute myeloic leukemia treated with high-dose Ara-C. Ten eyes developed fluorescein-negative punctate keratopathy, and were examined by slit lamp and in vivo confocal biomicroscopy at days 1, 3-4 and 9-14 after the beginning of ocular symptoms. RESULTS: In vivo confocal microscopy revealed disseminated highly reflective granular irregular intraepithelial elements in the central cornea, which affected about 3% of epithelial cells. At day 1 of symptoms, these elements were present only in the basal epithelial layer (median 275.5/mm(2)), at days 3-4 they were mainly found in the basal (187.5/mm(2)) but also in the apical layers (96/mm(2)), at days 9-14 they mainly presented in more superficial layers (115/mm(2) apically vs. 15.5/mm(2) in the basal layers). DISCUSSION: The intraepithelial distribution of cells with a granular cytosolic signal evolved over time, reflecting the migration of the necrotic basal cells to the wing cell layer and finally to apical epithelial layers. The desquamation of the necrotic cells is related to the resolution of symptoms, according to the period of the epithelial cell turnover. By confocal microscopy, we could follow the intraepithelial route of cells damaged by Ara-C in vivo.

West Indian (Caribbean) punctate keratopathy: clinical and in vivo confocal microscopical findings.

BACKGROUND: West Indian punctate keratopathy (WIPK) is characterized by stable, asymptomatic, white superficial corneal opacities. We examined this patient's lesions with in vivo confocal microscopy. METHODS: A 47-year-old North American male patient presented with bilateral, dense, punctate, white opacities of the corneal epithelium. Confocal microscopy was performed using the Heidelberg Retina Tomograph II/Rostock Cornea Module. RESULTS: The patient was of Afro-Caribbean descent. In vivo confocal microscopy showed hyporeflective areas with multiple small hyperreflective dots inside at the level of the basal corneal epithelium with an unaffected Bowman layer. There was no round cell infiltration. CONCLUSIONS: West Indian punctate keratopathy should be considered in the differential diagnosis of scattered spot-like opacities with a history of residence in the endemic West Indies. Inflammatory entities can be excluded by in vivo confocal microscopy.