RESUMO
Chain shortening of palmitic acid was examined in vivo by comparing oxidation rates of [1-13C]palmitate vs [16-13C]palmitate fed to four male subjects consuming a high-fat diet. For 9 d subjects were fed a diet of normal foods providing an energy intake equal to their estimated requirements. The diet provided (as energy) approximately 14% protein, 46% carbohydrate, and 40% fat at a P:S ratio of 0.25. Analysis of breath 13CO2 enrichment on day 3 permitted analysis of background 13C contribution from the test diet alone. On days 4 and 7 either [1-13C]palmitic acid or [16-13C]palmitic acid (9-13.5 mg/kg body wt) was fed with the breakfast meal. The whole-body rate of oxidation of [1-13C]palmitic acid was significantly greater than that observed for [16-13C]palmitic acid. These results suggest that up to 34% of dietary palmitic acid consumed may be subjected to extramitochondrial chain shortening.
Assuntos
Gorduras na Dieta/metabolismo , Ácidos Palmíticos/metabolismo , Adulto , Peso Corporal , Isótopos de Carbono , Gorduras na Dieta/administração & dosagem , Ingestão de Energia , Humanos , Masculino , Oxirredução , Ácido Palmítico , Ácidos Palmíticos/administração & dosagemRESUMO
The aim of the study was to determine the composition of flavour isolates from garlic and horse-radish, prepared by dichlorodifluoromethane extraction. Gas chromatography, with olfactory determination of the flavour of the resolved components, and gas chromatography combined with mass spectrometry were used. In the garlic extract 19 components comprising mono-, di-, and trisulphides and thiophene derivatives were detected. In the horse-radish extract 14 components including isothiocyanates, thiocyanates and cyanides were identified.
Assuntos
Aromatizantes/análise , Plantas/análise , Enxofre/análise , Cromatografia Gasosa , Alho/análise , Cromatografia Gasosa-Espectrometria de Massas , Plantas MedicinaisRESUMO
A method for preparation of natural flavour isolates from fruits, vegetables and herb spices, developed at the Institute of Fermentation Industry in Warsaw, was presented. It involves extraction of raw materials with CCl2F2 at 5 X 10(5) Pa. For the different groups of raw materials, three types of extracting apparatuses were developed. The quality of the preparations, technological parameters of their production, storage conditions and applications are the subject of several publications and non-published materials; therefore, the present paper is of an only general nature.