RESUMO
Peripheral blood progenitor cells (PBPC) were mobilised by recombinant human G-CSF (rhG-CSF) and cyclophosphamide, harvested by apheresis from 9 patients with NHL and stored at 4 degrees C without further manipulation. They were then reinfused after high-dose chemotherapy. We monitored the change in colony-forming units-granulocyte, macrophage (CFU-GM) proliferation as well as plasma glucose, lactate and pH levels over the period of the study. In an attempt to maintain CFU-GM numbers, rhG-CSF was added to storage bags at collection and 48 h later. Our observations suggest that cell viability is well maintained and that CFU-GM numbers rise over the first 48 h of storage before falling rapidly. Metabolic changes cause a fall in the pH and glucose levels with a reciprocal rise in plasma lactate. The addition of rhG-CSF at a concentration of 10 ng/ml to cells in storage showed no detectable benefit. Following storage for 96 h, 77% (SEM +/- 8%) of the initial CFU-GM remained.
Assuntos
Transplante de Células-Tronco Hematopoéticas/métodos , Remoção de Componentes Sanguíneos , Sobrevivência Celular , Fator Estimulador de Colônias de Granulócitos/farmacologia , Hematopoese , Células-Tronco Hematopoéticas/citologia , Humanos , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Preservação de Tecido/métodosRESUMO
The results from 40 patients who have undergone autologous bone marrow transplantation (ABMT) for relapsed or refractory Hodgkin's disease between March 1988 and September 1992 have been analysed. In contrast to our results in patients with relapsed HD, our results in patients with refractory HD are comparatively poor. Conventional salvage chemotherapy also seems inappropriate in these patients and we therefore believe they should be offered high-dose chemotherapy before their disease becomes refractory to conventionally scheduled regimens. Peripheral blood stem cell (PBSC) transplant now offers an attractive alternative to ABMT and may replace both intensive salvage chemotherapy and ABMT as the optimum treatment for patients who fail to respond to conventional chemotherapy regimens.
Assuntos
Transplante de Medula Óssea/estatística & dados numéricos , Doença de Hodgkin/terapia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Transplante de Medula Óssea/mortalidade , Terapia Combinada , Resistência a Medicamentos , Feminino , Doença de Hodgkin/mortalidade , Humanos , Tábuas de Vida , Masculino , Prognóstico , Indução de Remissão , Estudos Retrospectivos , Terapia de Salvação , Análise de Sobrevida , Resultado do TratamentoRESUMO
A monoclonal antibody-derived, dry blood grouping plate, based upon the simple disaccharide, trehalose, is described that is indefinitely stable at room temperature and was found to have a 99.8% accuracy when tested against a standard semiautomated assay. This plate can be used by personnel with no specific training to check the recorded A,B,O, and Rhesus blood type of potential transfusion recipients in the field and at the bedside. Trehalose-based reagents may be important for bedside testing and in developing countries where refrigeration is unreliable.
Assuntos
Anticorpos Monoclonais , Tipagem e Reações Cruzadas Sanguíneas/instrumentação , Imunoglobulina M , Trealose , Testes de Aglutinação , Tipagem e Reações Cruzadas Sanguíneas/métodos , Países em Desenvolvimento , Armazenamento de Medicamentos/métodos , Estudos de Avaliação como Assunto , Humanos , Fatores de TempoRESUMO
A case of severe intravascular haemolysis, due to an 'auto-anti-B antibody', and a case of transient haemolytic anaemia, due to an 'auto-anti-A antibody', following allogeneic organ transplantation, are reported. Both patients were receiving immunosuppressive therapy with cyclosporin A and were non-secretors of A, B and H substances.