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1.
J Exp Child Psychol ; 246: 106013, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38996742

RESUMO

Previous studies have established a negative correlation between economic deprivation and self-esteem; however, limited insights exist regarding the onset of children linking self-esteem to economic status. To investigate this, we examined 198 preschoolers (96 girls and 102 boys) and their parents (170 mothers and 28 fathers). We assessed children's implicit and explicit self-esteem, whereas parents' reported on both personal relative deprivation and the family's economic objective deprivation. In addition, we explored children's money knowledge as a moderator. Our findings reveal that preschoolers may connect their implicit self-esteem with family economic status; however, such connections require basic knowledge about money. We discuss potential explanations for the influence of family economic deprivation, specifically on the implicit-not explicit-self-esteem of preschoolers.


Assuntos
Autoimagem , Humanos , Feminino , Masculino , Pré-Escolar , Status Econômico , Pais/psicologia , Adulto , Família/psicologia
2.
Mikrochim Acta ; 190(10): 391, 2023 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-37704761

RESUMO

A original electrochemical sensing platform, based on screen-printed electrodes modification with plasma polymerized acrylonitrile (pp-AN) nanofilms is proposed. For that purpose, plasma-enhanced chemical vapor deposition (PECVD) process was conducted in a parallel plate (13.56 MHz) plasma reactor for 2 min with discharge power of 10 W. The surface topography and electrochemical properties of prepared sensors were investigated by X-ray photoelectron spectroscopy, scanning electron microscopy, energy dispersion spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. The electrochemical characteristics of pp-AN/SPCE and pp-AN/SPAuE sensors was investigated for model redox pair [Fe(CN)6]4-/3-. Conducted research confirmed the excellent chemical stability, durability, wide potential window, high signal-to-noise (S/N) ratio, and, most importantly, the ability to standardize the sensors. The pp-AN/SPCE sensor was applied to the determination of bupropion, an antidepressant drug whose intake has increased dramatically during the COVID-19 pandemic. The voltammetric response of pp-AN/SPCE for BUP was linear in two concentration ranges of 0.63-10.0 and 10.0-50.0 µmol L-1, with a detection limit of 0.21 µmol L-1. Satisfactory recoveries (96.2-102%) and good precision (RSD below 4.1%) obtained for environmental and biological samples confirmed the usefulness of the sensor for the analysis of various kinds of samples.

3.
PLoS One ; 19(9): e0309945, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39264915

RESUMO

Positive body image is crucial in fostering self-esteem, mental well-being, and positive social functioning. However, our understanding of its development and role in school-aged children remains limited due to a scarcity of measures available for this age group across various countries and cultures. To bridge this gap, the aim of this study was to adapt the Body Appreciation Scale-2 for Children (BAS-2C) for Polish children aged 8-13 years. A total of 206 children completed BAS-2C to measure positive body image, the Figures Rating Scale to measure body satisfaction, and the Self-Perception Profile for Children to measure self-esteem. Parents provided information about their child's weight and height, which enabled analysis of a child's BMI. The results showed that the Polish version of BAS-2C exhibited a unidimensional factor structure, invariance across sex and age groups, and also provided evidence of very good internal consistency, test-retest reliability, and construct validity. BAS-2C scores were correlated positively with Figures Rating Scale, physical appearance and global self-esteem and were related negatively to age and BMI index, and weight group. Moreover, our research indicates that in older children, there are stronger correlations between positive body image and both global and physical appearance self-esteem, compared to younger children. The Polish version of BAS-2C has excellent psychometric properties and is appropriate for use with children aged 8-13 years.


Assuntos
Imagem Corporal , Psicometria , Autoimagem , Humanos , Criança , Feminino , Masculino , Adolescente , Psicometria/métodos , Polônia , Imagem Corporal/psicologia , Inquéritos e Questionários , Reprodutibilidade dos Testes , Índice de Massa Corporal
4.
PLoS One ; 18(8): e0286028, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37531321

RESUMO

Proper communication with the public is crucial for encouraging private donors to make financial and non-financial donations to charities. This study compared the effectiveness of an advertising campaign that used a prevention framing for the charity's purpose and one that used a promotional framing. This experimental study was conducted online with 547 participants. The results showed that the advertising message highlighting the promotional goals of the campaign was more effective than the one based on prevention goals. This result was observed not only for the evaluation of the campaign and organization as well as behavioral intentions, but, crucially, also on the level of actual behavior.


Assuntos
Instituições de Caridade , Promoção da Saúde , Humanos , Promoção da Saúde/métodos , Objetivos , Intenção , Comunicação
5.
PLoS One ; 18(8): e0290512, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37619235

RESUMO

Materialistic tendencies may originate in early childhood, and previous research shows that even preschoolers differ in the degree of their materialism. The goal of this project was to develop and validate a new instrument that reliably measures materialism in preschool children (4-6 years old). A simple and universal Pictorial Materialism Test (PMT) was created and its psychometric properties were evaluated. The PMT consists of 32 items reflecting two dimensions: acquisition as the pursuit of happiness and success defined by possessions. To evaluate the psychometric properties of the PMT, a total of 204 Polish children aged 4-6 years were recruited for the study using an opportunity sampling method. To examine the reliability of the PMT, we calculated the internal consistency, confirmatory factor analysis (CFA), and test-retest intra-class correlation. To determine the convergent validity of the PMT, correlations with another measure of materialism in children (Happiness Collage) and with age were examined. The results confirmed that materialism can be reliably reported by preschool children and that the PMT has satisfactory (albeit moderate) psychometric properties. The instrument developed in this project is promising for further research because it has the potential to be used in research on materialism in preschool children around the world. In conclusion, we propose a preliminarily validated new Pictorial Materialism Test to measure general materialistic tendencies and their dimensions (possession-driven happiness and materially defined success) in 4-6-year-old children.


Assuntos
Felicidade , Humanos , Pré-Escolar , Criança , Reprodutibilidade dos Testes , Análise Fatorial
6.
Artigo em Inglês | MEDLINE | ID: mdl-35162822

RESUMO

During the COVID-19 pandemic, many factors have simultaneously affected people's psychological distress (PD). The most commonly studied types of factors have been those relating to health risks involving SARS-CoV-2 infection and sociodemographic factors. However, financial changes at both the national and global levels and these changes' influences on people's personal finances constitute another group of factors with the potential to cause symptoms of anxiety and depression. A correlation study of 1135 working adults in Poland was conducted to analyze the roles of a wide range of financial variables in explaining the extent of people's PD during the pandemic. Three groups of financial factors predicted PD over and above sociodemographic variables and COVID-19 health-related factors: a person's objective financial situation, their subjective financial situation, and their individual financial disposition, the last of these being the most important. The present study adds to the current state of knowledge by showing that financial variables explain a significant portion of variance in PD over and above sociodemographic and COVID-19 health-related factors. Moreover, the study also identified individual financial variables that were capable of predicting people's psychological distress during the pandemic.


Assuntos
COVID-19 , Angústia Psicológica , Adulto , Ansiedade/epidemiologia , Depressão/epidemiologia , Humanos , Pandemias , Polônia/epidemiologia , SARS-CoV-2 , Estresse Psicológico/epidemiologia
7.
Biochim Biophys Acta ; 1749(1): 133-41, 2005 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-15848144

RESUMO

Human topoisomerase I is a nuclear enzyme that catalyses DNA relaxation and phosphorylation of SR proteins. Topoisomerase I participates in several protein-protein interactions. We performed a proteomic analysis of protein partners of topoisomerase I. Two methods were applied to proteins of the nuclear extract of HeLa cells: a co-immunoprecipitation and an affinity chromatography combined with mass spectrometry. Complexes formed by topoisomerase I with its protein partners were immunoprecipitated by scleroderma anti-topoisomerase I antibodies. To identify binding sites for the protein partners, baits corresponding to fragments of topoisomerase I were constructed and used in the affinity chromatography. The N-terminal domain and the cap region of the core domain appeared to be the main regions that bound proteins. We identified 36 nuclear proteins that were associated with topoisomerase I. The proteins were mainly involved in RNA metabolism. We found 29 new and confirmed 7 previously identified protein partners of topoisomerase I. More than 40% proteins that associate with the cap region contain two closely spaced RRM domains. Docking calculations identified the RRM domains as a possible site for the interaction of these proteins with the cap region.


Assuntos
DNA Topoisomerases Tipo I/metabolismo , Proteínas Nucleares/metabolismo , DNA Topoisomerases Tipo I/análise , DNA Topoisomerases Tipo I/química , Células HeLa , Humanos , Imunoprecipitação , Conformação Proteica , Proteômica , RNA/metabolismo , Ribonucleoproteínas/análise , Ribonucleoproteínas/metabolismo
8.
Front Psychol ; 7: 609, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27199842

RESUMO

The psychological model of thinking about money assumes that implicit reminders of money lead to self-sufficient motivation. Previous research has demonstrated that children react to money in similar ways to adults. The priming of young children with money related concepts or images has negatively affected their social behavior and social preferences, leading them to make more individualist and less pro-social choices and be less willing to help others. The aim of this research was to investigate the positive influence of money activation on children's behavior. The participants were 6-8 year old children who do not yet fully understand the instrumental function of money due to their young age. Two experimental studies were performed, the first of which analyzed the effect of perseverance and performance on a challenging task and the second investigated preferences with respect to delaying gratification. Sixty-one children aged 6 took part in the first study and forty-six scout camp participants 6-8 years of age were involved in the second experiment. The results support the hypotheses concerning the effects of money activation stating that (1) money activation influences children's perseverance and effectiveness in difficult individual tasks, and that (2) it increases children's preferences for delayed gratification. These results suggest that money has a symbolic power which may exert both positive and negative effects on children's behavior. Since children between the ages of 6 and 8 do not understand the instrumental function of money fully, certain symbolic meanings of money may have been responsible for the money priming effects. The findings suggest that the symbolic function of money is more primal than its instrumental function and that it probably develops at an earlier stage in life.

9.
Front Psychol ; 7: 365, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27014168

RESUMO

In recent times, the changes occurring in the social role of women and men have been observed. Traditionally, the dominating social role of the woman was as housewife, and that of the man was focused on work and family maintenance. Nowadays, the social role of women is evolving in the direction of taking a profession, while increasingly men are taking care of the household. The main goal of the studies presented here was to verify how the activation of different social roles (traditional or non-traditional) may be reflected in women's financial and consumer choices. Three experimental studies were conducted. In the first study (n = 195 females), three different social roles of women - professional (non-traditional), housewife (traditional) and neutral (control) - were activated. The results showed that activating women's non-traditional social role increased their tendency to invest and decreased their propensity to save money compared to the activation of the traditional or neutral social role. The goal of the second study (n = 196 females) was to check whether, despite there being no differences in the level of consumption in the first study, can any differences be observed in the preference for the type of products chosen for consumption. The results showed that activating the non-traditional social role raised the propensity to spend funds on products and services for individual use and reduced the willingness to buy goods for collective use (shared with other members of the household). The purpose of the third study (n = 90 females) was to examine how different images of women appearing in advertisements may affect women's judgments of the advertised product. Women who watched the ad with woman in the non-traditional social role estimated the product quality, look, color and price higher that participants exposed to the advertisement presenting the woman in traditional or neutral social role. The present studies give some evidence that the new, non-traditional social role of women that is often presented in the media may affect women's everyday financial choices and judgments of products.

10.
Front Psychol ; 7: 1360, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27630611

RESUMO

People's preferences for risks have been a subject of interest to researchers in both the economy and psychology fields over the last few years. This has given rise to many important findings about the role of psychological factors that influence people's choices. The presented studies focused on the role of motivational systems (described by Higgins in the Regulatory Focus Theory) in explaining people's financial choices. The main goal was to examine the relationship between people's chronic promotion and prevention motivational system and their propensity to (1) invest, (2) undertake investment risks, and (3) assume financial risks in gambling tasks in both the gain and loss decision-making frame. Moreover, we aimed to investigate how chronic motivational systems confronted with situationally induced promotion and prevention motivation would affect people's propensity to invest and embrace financial risks. Two CAWI studies on a Polish national representative sample (N 1 = 1093; N 2 = 1096) were conducted. The second study consisted of two waves with a 2-week break. The studies provided evidence of higher chronic promotion motivation as well as higher prevention motivation associated with the propensity to invest; however, induced promotion motivation results in a lower propensity to invest compared to induced prevention motivation. Participants with an activated promotion system built more risky portfolios than individuals with an induced prevention system. Moreover, participants with a low chronic promotion system built more risky portfolios than individuals with a high promotion motivation system as long as their prevention system was also low. In terms of gambling decisions in both the gain and loss frame, a higher level of chronic promotion motivation and situationally induced promotion motivation were related to the preference for the non-sure option over the sure one.

11.
Biochem Biophys Res Commun ; 331(2): 398-403, 2005 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-15850773

RESUMO

DNA relaxation catalysed by topoisomerase I is based on the reversible DNA cleavage. The reaction is inhibited by binding of splicing protein SF2/ASF, a substrate for the kinase activity of topoisomerase I. In this paper, we show a novel binding site for SF2/ASF in the cap region of topoisomerase I (amino acids 215-433) which interacts with the region containing two closely spaced RRM domains of SF2/ASF (amino acids 1-194). The sites were defined by a set of pull-down experiments with isolated recombinant polypeptides. We also indicate that the novel site is responsible for the inhibition of DNA cleavage. The polypeptide containing tandem RRM domains inhibited DNA cleavage by topoisomerase I similarly as the complete SF2/ASF. Moreover, interaction between the tandem RRM domains and the cap region was not possible in the presence of DNA.


Assuntos
DNA Topoisomerases Tipo I/química , DNA Topoisomerases Tipo I/metabolismo , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/metabolismo , Sítios de Ligação , DNA/genética , DNA/metabolismo , Humanos , Ligação Proteica , Estrutura Terciária de Proteína , Proteínas de Ligação a RNA/genética
12.
Mol Biol Rep ; 29(4): 347-52, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12549820

RESUMO

Phage display procedure was applied to the N-terminal domain of human topoisomerase I. The consensus sequence identified for clones binding to the N-terminal domain was found in 35 human proteins that are either permanently or temporarily located in the nucleus. They are in majority involved in the DNA repair, transcription, RNA metabolism or cell cycle control. Four of identified proteins: Bub3 protein, Cockayne syndrome protein A, damaged DNA binding protein 2 and GRWD protein belong to WD-repeat proteins and their sequences recognized by the N-terminal domain are identically localized.


Assuntos
DNA Topoisomerases Tipo I/química , DNA Topoisomerases Tipo I/metabolismo , Biblioteca de Peptídeos , Sequência de Aminoácidos , Sequência Consenso , Humanos , Dados de Sequência Molecular , Peso Molecular , Ligação Proteica , Estrutura Terciária de Proteína
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