RESUMO
In a controlled study, 24 rabbits underwent bilateral division and immediate microsurgical anastomosis of the oviducts. Dexamethasone and promethazine were administered to 13 rabbits in a dose and route of administration similar to those used in clinical practice. Eleven control rabbits received a saline vehicle. Morbidity and mortality were encountered only in the dexamethasone and promethazine-treated group. Dexamethasone and promethazine did not appear to influence the formation of intraluminal and peritubal adhesions, histology, ultrastructure, patency, implantation, or pregnancy rates. The presence of intraluminal adhesions, as seen by scanning electron microscopy, was associated with relatively more efficient tubal function as measured by the ability of the oviducts to convey ova to intrauterine implantation sites. Collagen accumulation in the muscularis, as demonstrated by trichrome staining, was associated with relatively decreased tubal function.
Assuntos
Dexametasona/administração & dosagem , Tubas Uterinas/fisiopatologia , Prometazina/administração & dosagem , Animais , Colágeno , Implantação do Embrião/efeitos dos fármacos , Testes de Obstrução das Tubas Uterinas , Tubas Uterinas/cirurgia , Tubas Uterinas/ultraestrutura , Feminino , Fertilização/efeitos dos fármacos , Inflamação/patologia , Ovulação/efeitos dos fármacos , Gravidez , Coelhos , Aderências TeciduaisRESUMO
We have developed a method for producing chronic regional nerve ischemia in rats by creating proximal limb arteriovenous shunts. This procedure results in a 50 to 75% reduction in endoneurial blood flow within the distal sciatic nerve as measured by the iodoantipyrine method. Nerve conduction velocities in sciatic nerves ipsilateral to the shunt fell by 25 to 30% within 2 weeks after creation of the shunt and did not recover for up to 10 months after the procedure. Morphological studies of the ischemic nerves showed structural abnormalities at nodes of Ranvier and mild axonal atrophy. Neither segmental demyelination nor axonal degeneration were evident. These results indicate that reduced endoneurial blood flow, insufficient to cause infarction, may result in measurable functional and morphological abnormalities in peripheral nerves.