RESUMO
A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (approximately 7 Mohms) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.
Assuntos
Técnicas Biossensoriais/instrumentação , Canais Iônicos/química , Silício/química , Técnicas Biossensoriais/métodos , Capsaicina/farmacologia , Linhagem Celular , Humanos , Canais Iônicos/efeitos dos fármacos , Canais Iônicos/metabolismo , Rim/citologia , Rim/efeitos dos fármacos , Rim/metabolismo , Ligantes , Técnicas de Patch-Clamp/instrumentação , Técnicas de Patch-Clamp/métodos , Canais de Cátion TRPV/química , Canais de Cátion TRPV/efeitos dos fármacos , Canais de Cátion TRPV/metabolismoRESUMO
We have developed two basic technologies for fabrication of supported planar lipid bilayer membrane ion channel biosensors: a defect-free lipid bilayer formation on the substrate surface with electrode pores and a patterning technique for the hydrophobic self-assembled-monolayer to form the guard ring that reduces the lipid bilayer edge-leak current. The importance of the supported-membrane structure to achieve low noise and high-speed performance is suggested on the basis of the observed relation between the single-ion-channel current noise and the pore size.
Assuntos
Técnicas Biossensoriais/métodos , Eletroquímica/métodos , Gramicidina/química , Bicamadas Lipídicas/química , Microeletrodos , Nanoestruturas/química , Nanotecnologia/métodos , Técnicas Biossensoriais/instrumentação , Cristalização/métodos , Impedância Elétrica , Eletroquímica/instrumentação , Nanoestruturas/ultraestrutura , Nanotecnologia/instrumentação , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
Positioning the sensor cell on the micropore of the sensor chip and keeping it there during incubation are problematic tasks for incubation type planar patch clamp biosensors. To solve these problems, we formed on the Si sensor chip's surface a cell trapping pattern consisting of a lattice pattern with a round area 5 µm deep and with the micropore at the center of the round area. The surface of the sensor chip was coated with extra cellular matrix collagen IV, and HEK293 cells on which a chimera molecule of channel-rhodopsin-wide-receiver (ChR-WR) was expressed, were then seeded. We examined the effects of this cell trapping pattern on the biosensor's operation. In the case of a flat sensor chip without a cell trapping pattern, it took several days before the sensor cell covered the micropore and formed an almost confluent state. As a result, multi-cell layers easily formed and made channel current measurements impossible. On the other hand, the sensor chip with cell trapping pattern easily trapped cells in the round area, and formed the colony consisted of the cell monolayer covering the micropore. A laser (473 nm wavelength) induced channel current was observed from the whole cell arrangement formed using the nystatin perforation technique. The observed channel current characteristics matched measurements made by using a pipette patch clamp.