RESUMO
We've conducted the analysis of both traditional and new methods of laboratory diagnosis to estimate the severity and damage of target organs during Hemorrhagic Fever with Renal Syndrome (HFRS). It was shown that thrombocytopenia corresponds to the severity of the disease and correlates with the severity of renal failure. The level of C-reactive protein reflects the activity of the inflammatory process and correlates with the characteristic laboratory criteria of the disease. Blood serum levels of alveomucine in patients with HFRS more than 45.0 U/ml indicates lung damage. HFRS on the territory of the Bashkortostan Republic is caused by the serotype of Puumala hantavirus; dependence of the form of the disease on the content of antiviral antibodies is not revealed.
Assuntos
Febre Hemorrágica com Síndrome Renal/diagnóstico , Virus Puumala , Anticorpos Antivirais/sangue , Bashkiria , Proteína C-Reativa/análise , Técnicas de Laboratório Clínico , Humanos , Trombocitopenia/diagnóstico , Trombocitopenia/virologiaRESUMO
For a differentiation of clinical forms of ixodic tick-borne borreliosis clinical laboratory assessment of features of the most often defined hemostasis and complete blood count (CBC) indicators is carried out. Ixodic tick-borne borreliosis in the territory of the Republic of Bashkortostan is characterized by mainly erythematous forms of a disease with a medium-weight current. At the same time some increase in quantity of platelets was noted that could be caused by irritation of a megakaryocytic sprout of bone marrow in the conditions of infectious process and have compensatory character whereas other indicators of CBC and hemostasis at this clinical form practically didn't change. Not numerous cases of the ixodic tick-borne borreliosis non-erythematous forms clinically proceeded more hard, with significantly more expressed toxicinflammatory syndrome. At the same time it was followed by significant shifts in biochemical parameters, indicators of CBC and hemostasis. The clinical laboratory features of erythematous and non-erythematous forms of ixodic tick-borne borreliosis revealed as a result of the conducted researches reflect character of a course of disease and can serve for assessment of severity, the forecast of the infection and justification of this treatment.
Assuntos
Ixodes , Doença de Lyme/diagnóstico , Doença de Lyme/patologia , Animais , HumanosRESUMO
The results of dehelmintization were studied in 127 patients with the intestinal stage of ascariasis. Its diagnosis was established by the Kato test. The ovoscopic technique was combined with the trend of proinflammatory cytokines and the grade of dysbacteriosis. There was a strong direct correlation of the high levels of proinflammatory cytokines and the high-grade dysbacteriosis with the inefficiency of expulsion of the parasite.
Assuntos
Anti-Helmínticos/uso terapêutico , Ascaríase/tratamento farmacológico , Ascaris/isolamento & purificação , Convalescença , Adolescente , Adulto , Animais , Ascaríase/diagnóstico , Ascaríase/imunologia , Ascaríase/microbiologia , Bifidobacterium/isolamento & purificação , Citocinas/análise , Feminino , Humanos , Interleucina-1/análise , Interleucina-6/análise , Lactobacillus/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Contagem de Ovos de Parasitas , Fator de Necrose Tumoral alfa/análiseRESUMO
The levels of the proinflammatory cytokines including interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) was investigated in 73 children in the age range from 3 to 17 years with slight and medium-heavy diarrheal illness caused by pathogenic and conditionally pathogenic entrobacteria. Strong positive correlation was found between: the levels of IL-1beta, TNF-alpha and intoxication rate (r=0.65 and r=0.49); height of temperature and duration of fever (r=0.86 and r=0.66); dyspepsia (r=0.48 and r=0.41); diarrhea (r=0.37 and r=0.54) and changes in blood including number of leucocytes (r=0.40 and r=0.52) and level of erythrocyte sedimentation rate (ESR) (r=0.65 and r=0.52). Medium positive relationship between the level of IL-6 and intoxication rate (r=0.40 and r=0.52), height of temperature and duration of fever (r=0.45), changes in blood including ESR (r=0.42) and number of leucocytes (r=0.46) was shown. There was a strong positive correlation between IL-1beta and TNF-alpha (r=0.74), between IL-6 and TNF-alpha (r=0.71) and a medium positive correlation between IL-1beta and IL-6 (r=0.61). Considerable decrease in concentration of all cytokines during early period of convalescent at disease with no complications was revealed. Change in concentration of IL-1beta and TNF-alpha determines both intoxication rate and fever. High level of IL-6 is related with complications and lingering course of disease.
Assuntos
Disenteria Bacilar/sangue , Disenteria Bacilar/patologia , Enterobacteriaceae/imunologia , Interleucina-1beta/sangue , Interleucina-6/sangue , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/metabolismo , Adolescente , Sedimentação Sanguínea , Criança , Pré-Escolar , Diarreia/patologia , Disenteria Bacilar/microbiologia , Febre/patologia , Imunofluorescência , Humanos , Contagem de Leucócitos , Leucocitose/patologia , Fatores de TempoRESUMO
The data of pathogenicity factors of opportunistic enterobacteria, including Klebsiella, Enterobacter, Citrobacter, Proteus, Providencia and Hafnia species are submitted. The genetic control and a role of pathogenicity factors of opportunistic enterobacteria in development of diarrhea syndrome are presented. Data about adhesins, hemolysins, cytotoxic necrotizing factors and bacterial modulins are described. The characteristic of cytotonic and cytotoxic enterotoxins, including LT, ST, Shiga-like and cytolethal toxins, and mechanisms of diarrheagenic action are analysed. The role of bacterial lipopolysaccharide (endotoxin) and induction of locally synthesized proinflammatory cytokins in pathogenisis of diarrhea are discussed.
Assuntos
Diarreia/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Enterobacteriaceae/patogenicidade , Fatores de Virulência/fisiologia , Adesinas Bacterianas/genética , Proteínas de Bactérias/fisiologia , Citotoxinas/fisiologia , Endotoxinas/fisiologia , Enterobacteriaceae/metabolismo , Enterotoxinas/fisiologia , Proteínas Hemolisinas/fisiologia , HumanosRESUMO
In the process of examination of 89 children from different age groups with diarrheal disease caused by bacteria from Enterobacteriaceae family 89 microorganisms were isolated including Klebsiella spp. (37 isolates), Citrobacter spp. (9 isolates), Enterobacter spp. (17 isolates), Hafnia alvei (1 isolate), Morganella morganii (11 isolates), Proteus spp. (14 isolates). Presence of genes associated with pathogenicity islands (PAIS): hlyA, hlyB (hemolysin), sfaG (fimbria antigen type S), cnf1 (cytotoxic necrotizing factor 1), estB (heat-stable enterotoxin B)were studied in these cultures by PCR. It was found that 32.6% of examined isolates had fragments of PAIS's genes--hlyA was detected in 9 cases (10.1%), hlyB--in 10 cases (11.2%), sfaG --in 8 cases (9%), cnf1--in 9 cases (10.1%), and estB--in 3 cases (3.4%). Positive correlation between genetic determinants hlyB and cnf1 as well as hlyA and sfaG was found while estB was not associated with other genes. Weak positive correlation between presence of sfaG and resistance to tetracycline and chloramphenicol was detected. Factors coded by revealed determinants of PAIS can play a role in the development of diarrheal syndrome.
Assuntos
Enterite/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Enterobacteriaceae/genética , Enterobacteriaceae/patogenicidade , Genes Bacterianos , Ilhas Genômicas/genética , Doença Aguda , Adesinas Bacterianas/genética , Adolescente , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Toxinas Bacterianas/genética , Proteínas de Transporte , Criança , Cloranfenicol/farmacologia , Citotoxinas/genética , Diarreia/microbiologia , Farmacorresistência Bacteriana , Enterobacteriaceae/classificação , Enterobacteriaceae/efeitos dos fármacos , Proteínas de Escherichia coli/genética , Marcadores Genéticos , Proteínas Hemolisinas/genética , Humanos , Especificidade da Espécie , Tetraciclina/farmacologia , Fatores de Virulência/genéticaAssuntos
Antivirais/uso terapêutico , Encefalite Transmitida por Carrapatos/tratamento farmacológico , Febre Hemorrágica com Síndrome Renal/tratamento farmacológico , Indutores de Interferon/uso terapêutico , Compostos de Iodo/uso terapêutico , Pirazolonas/uso terapêutico , Adulto , Idoso , Encefalite Transmitida por Carrapatos/epidemiologia , Encefalite Transmitida por Carrapatos/prevenção & controle , Feminino , Humanos , Interferons , Masculino , Pessoa de Meia-Idade , Federação Russa/epidemiologia , Resultado do TratamentoRESUMO
The effect of eracond on some parameters of immunity and allergic reactions was studied in experiments on mice and guinea pigs. The drug was found to possess an immunomodulating effect.
Assuntos
Adjuvantes Imunológicos/farmacologia , Medicago sativa/química , Animais , Formação de Anticorpos/efeitos dos fármacos , Relação Dose-Resposta a Droga , Cobaias , Imunidade Celular/efeitos dos fármacos , Imunização , Masculino , Camundongos , Camundongos Endogâmicos CBA , Anafilaxia Cutânea Passiva/efeitos dos fármacos , Extratos Vegetais/farmacologiaRESUMO
Hypophyseo-adrenal and hypophyseo-thyroid systems were studied in 102 patients aged 16-55 (88 males and 14 females) who suffered from hemorrhagic fever with renal syndrome. Measurements of serum levels of ACTH, hydrocortisone, TTH, T3 and T4 recorded supernormal concentrations of ACTH, hydrocortisone and TTH against subnormal concentrations of T3 and T4 in oliguric and polyuric periods of the disease. In convalescence TTH, T3 and T4 returned to normal levels, while ACTH and hydrocortisone were lower than in controls. It is inferred that in hemorrhagic fever with renal syndrome the activity of the system hypophysis-adrenals in convalescence is declined, whereas hypophysis-thyroid interaction is disturbed at the height of the disease.
Assuntos
Febre Hemorrágica com Síndrome Renal/fisiopatologia , Hipófise/fisiopatologia , Sistema Hipófise-Suprarrenal/fisiopatologia , Glândula Tireoide/fisiopatologia , Adolescente , Adulto , Convalescença , Feminino , Febre Hemorrágica com Síndrome Renal/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Oligúria/sangue , Oligúria/fisiopatologia , Poliúria/sangue , Poliúria/fisiopatologiaRESUMO
IgG, IgE, IgA, IgM levels were measured in patients with yersiniosis (generalized or mixed variant). Acute period of the disease (week 1-2) was associated with elevated levels of IgE in 59.1% of the patients which returned to normal on week 4-6. Time course changes in IgA, IgG and IgM concentrations were related to the synthesis of specific antibodies. IgE level rise can be considered a defence mechanism contributing to elimination of yersinia antigens from the body.