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1.
Microb Pathog ; 123: 18-23, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29906540

RESUMO

The present context was aimed to investigate the antibacterial potency of aqueous extract of coriander (Coriandrum sativum L.) leaves against bacterial pathogens isolated from the organs associated with digestive system of rabbit. This study also evaluated the influence of varied doses of aqueous extract of C. sativum (AECS) leaves on in vitro gas production (GP), methane (CH4) production, and some other pivotal fermentation parameters from caecal sample of rabbits. The pathogenic bacteria were isolated from mouth, caecum, and anus of rabbits, and further identified through morphological, biochemical, and molecular tools. The growth inhibitory characteristics of AECS against pathogens were determined using disc diffusion assay. Surprisingly, the result revealed lack of antibacterial potential at tested concentrations. Further, in order to demonstrate the in vitro GP and fermentation parameters in rabbits, four treatments comprising of 0, 0.6, 1.2, and 1.8 mL extract/g dry matter (DM) of AECS were used. Results showed no linear or quadratic effect (P > 0.05) on in vitro GP and CH4 production after the supplementation of AECS in the feeding diet. However, the inclusion of AECS at the concentration of 1.8 mL/g DM exhibited the lowest asymptotic CH4 production and initial delay prior to CH4 production. Similarly, the addition of AECS at 1.8 mL/g DM concentration reduced asymptotic GP as well as CH4 production, and improved fermentation parameters of rabbits when compared with the control and other tested doses. In a nutshell, the tested doses of AECS showed lack of antibacterial trait against the pathogenic bacteria isolated from mouth, caecum, and anus of rabbits. Besides, the AECS exhibited the unique potentiality of reducing GP and improving diversified fermentation parameters in rabbits, thereby suggesting its plausible role as an alternative to commercially available growth promoters in livestock industries.


Assuntos
Ceco/metabolismo , Coriandrum/química , Fermentação/efeitos dos fármacos , Metano/biossíntese , Extratos Vegetais/farmacologia , Canal Anal/microbiologia , Animais , Ceco/microbiologia , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Boca/microbiologia , Pantoea/efeitos dos fármacos , Pantoea/isolamento & purificação , Coelhos , Shigella/efeitos dos fármacos , Shigella/isolamento & purificação , Yersinia pestis/efeitos dos fármacos , Yersinia pestis/isolamento & purificação
2.
Pathogens ; 12(1)2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36678472

RESUMO

Goat production in Mexico is an important economic activity that is affected by different gastrointestinal nematode (GIN) species. GINs resistant to commercial anthelmintics have been reported. Plant extracts or agro-industrial by-products, such as coffee pulp, have been proposed as control alternatives, given their secondary metabolite content. The aim of the present study was to determine the anthelmintic activity of the hydroalcoholic extract of coffee pulp against benzimidazole-resistant GINs. Stool samples were collected from goats, from which GIN eggs were identified and quantified. Molecular techniques confirmed the genus of GINs and their benzimidazole resistance profile. The percentage of egg hatching inhibition (% EHI) and larval mortality (% LM) with the hydroalcoholic extract of coffee pulp was determined at concentrations from 200 to 0.39 mg/mL. The genera Haemonchus spp. and Trichostrongylus spp. were identified, and the presence of the ß-tubulin gene mutation, associated with benzimidazole (BZ) resistance, was determined. Hydroalcoholic extract of coffee pulp inhibited 100% of egg hatching at 200 and 100 mg/mL, with no larvicidal effect at the evaluated concentrations.

3.
J Wildl Dis ; 59(3): 524-527, 2023 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-37167076

RESUMO

We examined 82 fresh fecal samples from American White Pelican (Pelecanus erythrorhynchos) at San Pablo Autopan, Toluca Valley, central Mexico, December 2021 to April 2022. Eggs of Contracaecum spp. were observed in 100%, Capillaria spp. in two (2.5%), Strongyloides spp. in eight (9.7%), and coccidial oocysts in 12 (15%).


Assuntos
Ascaridoidea , Doenças das Aves , Animais , Doenças das Aves/epidemiologia , Doenças das Aves/parasitologia , México/epidemiologia , Aves/parasitologia
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