RESUMO
Dengue virus type 3 genotype I was detected in Brazil during epidemics in 2002-2004. To confirm this finding, we identified this virus genotype in naturally infected field-caught Aedes aegypti mosquitoes and eggs. Results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance.
Assuntos
Aedes/virologia , Vírus da Dengue/genética , Dengue/epidemiologia , Óvulo/virologia , Animais , Brasil/epidemiologia , Dengue/transmissão , Dengue/virologia , Vírus da Dengue/isolamento & purificação , Feminino , Variação Genética , Genoma Viral , Humanos , Transmissão Vertical de Doenças Infecciosas , Masculino , Filogenia , RNA Viral/análise , RNA Viral/genética , Alinhamento de Sequência , Análise de Sequência de RNA , Especificidade da Espécie , Fatores de TempoRESUMO
Dengue is currently regarded as a major public health problem worldwide. In a hyperendemic region during an outbreak, we detected the co-circulation of all Dengue virus (DENV) serotypes including two different genotypes of DENV-3 and DENV-4, and concurrent infections with up to three serotypes were identified in symptomatic patients. A total of 49 acute phase plasma samples from patients clinically suspected of dengue were collected during the 4 weeks of May 2013. DENV-1-4 was detected by reverse transcriptase semi-nested polymerase chain reaction in 33 samples (67.3%), of which 26 DNA fragments were sequenced. Twenty samples (76.9%) were identified with a single DENV serotype and six (23.1%) with more than one serotype. DENV-3 was the predominant serotype of the outbreak. On the basis of phylogenetic analyses, DENV-1 isolates belong to genotype V, DENV-2 to American-Asian genotype, DENV-3 to genotypes I and III, and DENV-4 to genotypes I and II.