Plastoquinone pool redox state and control of state transitions in Chlamydomonas reinhardtii in darkness and under illumination.

Movement of LHCII between two photosystems has been assumed to be similarly controlled by the redox state of the plastoquinone pool (PQ-pool) in plants and green algae. Here we show that the redox state of the PQ-pool of Chlamydomonas reinhardtii can be determined with HPLC and use this method to compare the light state in C. reinhardtii with the PQ-pool redox state in a number of conditions. The PQ-pool was at least moderately reduced under illumination with all tested types of visible light and oxidation was achieved only with aerobic dark treatment or with far-red light. Although dark incubations and white light forms with spectral distribution favoring one photosystem affected the redox state of PQ-pool differently, they induced similar Stt7-dependent state transitions. Thus, under illumination the dynamics of the PQ-pool and its connection with light state appears more complicated in C. reinhardtii than in plants. We suggest this to stem from the larger number of LHC-units and from less different absorption profiles of the photosystems in C. reinhardtii than in plants. The data demonstrate that the two different control mechanisms required to fulfill the dual function of state transitions in C. reinhardtii in photoprotection and in balancing light utilization are activated via different means.

Acclimation of Chlamydomonas reinhardtii to extremely strong light.

Most photosynthetic organisms are sensitive to very high light, although acclimation mechanisms enable them to deal with exposure to strong light up to a point. Here we show that cultures of wild-type Chlamydomonas reinhardtii strain cc124, when exposed to photosynthetic photon flux density 3000 µmol m-2 s-1 for a couple of days, are able to suddenly attain the ability to grow and thrive. We compared the phenotypes of control cells and cells acclimated to this extreme light (EL). The results suggest that genetic or epigenetic variation, developing during maintenance of the population in moderate light, contributes to the acclimation capability. EL acclimation was associated with a high carotenoid-to-chlorophyll ratio and slowed down PSII charge recombination reactions, probably by affecting the pre-exponential Arrhenius factor of the rate constant. In agreement with these findings, EL acclimated cells showed only one tenth of the 1O2 level of control cells. In spite of low 1O2 levels, the rate of the damaging reaction of PSII photoinhibition was similar in EL acclimated and control cells. Furthermore, EL acclimation was associated with slow PSII electron transfer to artificial quinone acceptors. The data show that ability to grow and thrive in extremely strong light is not restricted to photoinhibition-resistant organisms such as Chlorella ohadii or to high-light tolerant mutants, but a wild-type strain of a common model microalga has this ability as well.

Degradation of chlorophyll and synthesis of flavonols during autumn senescence-the story told by individual leaves.

Autumn senescence of deciduous trees is characterized by chlorophyll degradation and flavonoid synthesis. In the present study, chlorophyll and flavonol contents were measured every morning and evening during the whole autumn with a non-destructive method from individual leaves of Sorbus aucuparia, Acer platanoides, Betula pendula and Prunus padus. In most of the studied trees, the chlorophyll content of each individual leaf remained constant until a phase of rapid degradation commenced. The fast phase lasted only ~1 week and ended with abscission. In S. aucuparia, contrary to the other species, the chlorophyll content of leaflets slowly but steadily decreased during the whole autumn, but rapid chlorophyll degradation commenced only prior to leaflet abscission also in this species. An increase in flavonols commonly accompanied the rapid degradation of chlorophyll. The results may suggest that each individual tree leaf retains its photosynthetic activity, reflected by a high chlorophyll content, until a rapid phase of chlorophyll degradation and flavonoid synthesis begins. Therefore, in studies of autumn senescence, leaves whose chlorophyll content is decreasing and leaves with summertime chlorophyll content (i.e. the leaves that have not yet started to degrade chlorophyll) should be treated separately.