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1.
Cell ; 136(6): 1073-84, 2009 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-19303850

RESUMO

Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin substrates of the essential nucleosome acetyltransferase of H4 (NuA4) complex. Among these, acetylation sites (Lys19 and 514) of phosphoenolpyruvate carboxykinase (Pck1p) were determined by tandem mass spectrometry. Acetylation at Lys514 was crucial for enzymatic activity and the ability of yeast cells to grow on nonfermentable carbon sources. Furthermore, Sir2p deacetylated Pck1p both in vitro and in vivo. Loss of Pck1p activity blocked the extension of yeast chronological life span caused by water starvation. In human hepatocellular carcinoma (HepG2) cells, human Pck1 acetylation and glucose production were dependent on TIP60, the human homolog of ESA1. Our findings demonstrate a regulatory function for the NuA4 complex in glucose metabolism and life span by acetylating a critical metabolic enzyme.


Assuntos
Gluconeogênese , Histona Acetiltransferases/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Acetilação , Técnicas de Silenciamento de Genes , Glucose/metabolismo , Histona Acetiltransferases/genética , Histona Desacetilases/metabolismo , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Lisina Acetiltransferase 5 , Complexos Multiproteicos/metabolismo , Fosfoenolpiruvato Carboxiquinase (GTP)/metabolismo , Análise Serial de Proteínas , Sirtuínas/metabolismo , Água/metabolismo
2.
Mol Cell Biol ; 26(14): 5226-36, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16809761

RESUMO

Transcription in eukaryotes is governed in part by histone acetyltransferase (HAT)- and histone deacetylase (HDAC)-containing complexes that are recruited via activators and repressors, respectively. Here, we show that the Sin3/HDAC and N-CoR/SMRT corepressor complexes repress transcription from histone H3- and/or H4-acetylated nucleosomal templates in vitro. Repression of histone H3-acetylated templates was completely dependent on the histone deacetylase activity of the corepressor complexes, whereas this activity was not required to repress H4-acetylated templates. Following deacetylation, both complexes become stably anchored in a repressor-independent manner to nucleosomal templates containing hypoacetylated histone H3, but not H4, resulting in dominance of repression over activation. The observed stable anchoring of corepressor complexes casts doubt on the view of a dynamic balance between readily exchangeable HAT and HDAC activities regulating transcription and implies that pathways need to be in place to actively remove HDAC complexes from hypoacetylated promoters to switch on silent genes.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Histona Desacetilases/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Repressoras/metabolismo , Acetilação , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação , Cromatina/metabolismo , DNA/genética , DNA/metabolismo , Proteínas de Ligação a DNA/química , Retroalimentação , Células HeLa , Histona Desacetilases/química , Histonas/química , Histonas/genética , Histonas/metabolismo , Humanos , Técnicas In Vitro , Dados de Sequência Molecular , Complexos Multiproteicos , Proteínas Nucleares/química , Correpressor 1 de Receptor Nuclear , Correpressor 2 de Receptor Nuclear , Nucleossomos/metabolismo , Regiões Promotoras Genéticas , Proteínas Repressoras/química , Saccharomyces cerevisiae/metabolismo , Complexo Correpressor Histona Desacetilase e Sin3 , Transcrição Gênica , Xenopus
3.
Trends Biochem Sci ; 29(3): 127-35, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15003270

RESUMO

Chromatin packages DNA tightly into the eukaryotic nucleus and maintains its proper functioning. Recent studies suggest the existence of two distinct mechanisms of progression of RNA polymerases through chromatin. The first is characteristic of eukaryotic RNA polymerase III, bacteriophage RNA polymerases, and probably ATP-dependent chromatin remodeling complexes. In this mechanism, nucleosomes are translocated without release of the octamer into solution. By contrast, transcription by RNA polymerase II (Pol II) involves displacement of one H2A-H2B dimer. Nucleosomes can present a barrier for transcribing Pol II that can be regulated in vivo. Analysis of the mechanisms of transcription through chromatin should provide important information about mechanisms of chromatin remodeling and gene regulation at the level of transcript elongation.


Assuntos
Cromatina/metabolismo , RNA Polimerases Dirigidas por DNA/metabolismo , Trifosfato de Adenosina/metabolismo , DNA/metabolismo , RNA Polimerases Dirigidas por DNA/química , Humanos , Nucleossomos/metabolismo , Transcrição Gênica
4.
Mol Cell Biol ; 28(8): 2840-9, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18268010

RESUMO

Histone modifications occur in precise patterns and are proposed to signal the recruitment of effector molecules that profoundly impact chromatin structure, gene regulation, and cell cycle events. The linked modifications serine 10 phosphorylation and lysine 14 acetylation on histone H3 (H3S10phK14ac), modifications conserved from Saccharomyces cerevisiae to humans, are crucial for transcriptional activation of many genes. However, the mechanism of H3S10phK14ac involvement in these processes is unclear. To shed light on the role of this dual modification, we utilized H3 peptide affinity assays to identify H3S10phK14ac-interacting proteins. We found that the interaction of the known phospho-binding 14-3-3 proteins with H3 is dependent on the presence of both of these marks, not just phosphorylation alone. This is true of mammalian 14-3-3 proteins as well as the yeast homologues Bmh1 and Bmh2. The importance of acetylation in this interaction is also seen in vivo, where K14 acetylation is required for optimal Bmh1 recruitment to the GAL1 promoter during transcriptional activation.


Assuntos
Proteínas 14-3-3/metabolismo , Histonas/metabolismo , Acetilação , Calorimetria , Células HeLa , Humanos , Fosforilação , Fosfosserina/metabolismo , Regiões Promotoras Genéticas/genética , Ligação Proteica , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo
5.
Mol Cell ; 27(2): 275-288, 2007 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-17643376

RESUMO

Histone modifications play an important role in transcription. We previously studied histone H2B ubiquitylation on lysine 123 and subsequent deubiquitylation by SAGA-associated Ubp8. Unlike other histone modifications, both the addition and removal of ubiquitin are required for optimal transcription. Here we report that deubiquitylation of H2B is important for recruitment of a complex containing the kinase Ctk1, resulting in phosphorylation of the RNA polymerase II (Pol II) C-terminal domain (CTD), and for subsequent recruitment of the Set2 methyltransferase. We find that Ctk1 interacts with histones H2A and H2B, and that persistent H2B ubiquitylation disrupts these interactions. We further show that Ubp8 enters the GAL1 coding region through an interaction with Pol II. These findings reveal a mechanism by which H2B ubiquitylation acts as a barrier to Ctk1 association with active genes, while subsequent deubiquitylation by Ubp8 triggers Ctk1 recruitment at the appropriate point in activation.


Assuntos
Endopeptidases/metabolismo , Histonas/metabolismo , Proteínas Quinases/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Transativadores/metabolismo , Ubiquitina/metabolismo , Endopeptidases/química , Endopeptidases/genética , Genes Fúngicos , Histonas/química , Histonas/genética , Modelos Biológicos , Complexos Multiproteicos , Nucleossomos/metabolismo , Fases de Leitura Aberta , Proteínas Quinases/química , Proteínas Quinases/genética , RNA Polimerase II/química , RNA Polimerase II/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/genética , Transativadores/química , Transativadores/genética , Transcrição Gênica
6.
Mol Cell ; 18(1): 97-108, 2005 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-15808512

RESUMO

In the cell, RNA polymerase II (pol II) efficiently transcribes DNA packaged into nucleosomes, but in vitro encounters with the nucleosomes induce catalytic inactivation (arrest) of the pol II core enzyme. To determine potential mechanisms making nucleosomes transparent to transcription in vivo, we analyzed the nature of the nucleosome-induced arrest. We found that the arrests have been detected mostly at positions of strong intrinsic pause sites of DNA. The transient pausing makes pol II vulnerable to arrest, which involves backtracking of the elongation complex for a considerable distance on DNA. The histone-DNA contacts reestablished in front of pol II stabilize backtracked conformation of the polymerase. In agreement with this mechanism, blocking of backtracking prevents nucleosome-induced arrest. Transcript cleavage factor TFIIS reactivates the backtracked complexes and promotes pol II transcription through the nucleosome. Our findings establish the crucial role of elongation factors that suppress pol II pausing and backtracking for transcription in the context of chromatin.


Assuntos
Nucleossomos/genética , RNA Polimerase II/metabolismo , Sequência de Bases , DNA/genética , Histonas/metabolismo , Dados de Sequência Molecular , Nucleossomos/metabolismo , Transcrição Gênica , Fatores de Elongação da Transcrição/metabolismo
7.
Methods ; 33(1): 18-24, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15039083

RESUMO

Transcription through the nucleosome by Saccharomyces cerevisiae RNA polymerase II (Pol II) is characterized by an almost absolute block to transcription at physiological ionic strength and displacement of one H2A/H2B dimer to form a hexasome [Mol. Cell 9 (2002) 541]. In previous studies of Pol II transcription through chromatin, templates containing nucleosomes in multiple positions were used. These templates do not allow detailed analysis of the mechanism of transcription through chromatin. Here, we describe the development of a new template that is only long enough to accommodate a single nucleosome position along the DNA so that all of the templates are identical and allow for more in-depth analysis. After ligation of the nucleosome to promoter DNA or assembled elongation complexes, the mechanism of transcription through this uniquely positioned nucleosome by various RNA polymerases can be analyzed.


Assuntos
DNA/metabolismo , Nucleossomos/genética , Enzimas de Restrição do DNA , Eletroforese em Gel de Poliacrilamida/métodos , Nucleossomos/metabolismo , Regiões Promotoras Genéticas , RNA Polimerase II/metabolismo , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Transcrição Gênica/fisiologia
8.
Mol Cell ; 9(3): 541-52, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11931762

RESUMO

RNA polymerase II (Pol II) must transcribe genes in a chromatin environment in vivo. We examined transcription by Pol II through nucleosome cores in vitro. At physiological and lower ionic strengths, a mononucleosome imposes a strong block to elongation, which is relieved at increased ionic strength. Passage of Pol II causes a quantitative loss of one H2A/H2B dimer but does not alter the location of the nucleosome. In contrast, bacteriophage SP6 RNA polymerase (RNAP) efficiently transcribes through the same nucleosome under physiological conditions, and the histone octamer is transferred behind SP6 RNAP. Thus, the mechanisms for transcription through the nucleosome by Pol II and SP6 RNAP are clearly different. Moreover, Pol II leaves behind an imprint of disrupted chromatin structure.


Assuntos
Histonas/metabolismo , Nucleossomos/metabolismo , RNA Polimerase II/metabolismo , Transcrição Gênica/fisiologia , RNA Polimerases Dirigidas por DNA/metabolismo , Dimerização , Substâncias Macromoleculares , Modelos Genéticos , Oligonucleotídeos/genética , Oligonucleotídeos/metabolismo
9.
J Biol Chem ; 278(38): 36148-56, 2003 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-12851391

RESUMO

We have previously shown that nucleosomes act as a strong barrier to yeast RNA polymerase II (Pol II) in vitro and that transcription through the nucleosome results in the loss of an H2A/H2B dimer. Here, we demonstrate that Escherichia coli RNA polymerase (RNAP), which never encounters chromatin in vivo, behaves similarly to Pol II in all aspects of transcription through the nucleosome in vitro. The nucleosome-specific pausing pattern of RNAP is comparable with that of Pol II. At physiological ionic strength or lower, the nucleosome blocks RNAP progression along the template, but this barrier can be relieved at higher ionic strength. Transcription through the nucleosome by RNAP results in the loss of an H2A/H2B dimer, and the histones that remain in the hexasome retain their original positions on the DNA. The results were similar for elongation complexes that were assembled from components (oligonucleotides and RNAP) and elongation complexes obtained by initiation from the promoter. The data suggest that eukaryotic Pol II and E. coli RNAP utilize very similar mechanisms for transcription through the nucleosome. Thus, bacterial RNAP can be used as a suitable model system to study general aspects of chromatin transcription by Pol II. Furthermore, the data argue that the general elongation properties of polymerases may determine the mechanism used for transcription through the nucleosome.


Assuntos
Proteínas de Bactérias/metabolismo , RNA Polimerases Dirigidas por DNA/química , Nucleossomos/enzimologia , RNA Polimerase II/química , Transcrição Gênica , Cromatina/metabolismo , Escherichia coli/enzimologia , Escherichia coli/metabolismo , Histonas/química , Íons , Modelos Genéticos , Nucleossomos/metabolismo , RNA Polimerase II/metabolismo
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