RESUMO
OBJECTIVE: To study the expression of long nonîcoding RNA (lncRNA) H19 in human prostate cancer tissue and its effect on the glycometabolism and growth of human prostate cancer cells. METHODS: Realîtime quantitative RTîPCR (qRTîPCR) was employed to detect the expression of lncRNA H19 in human prostate tissues from 20 patients with prostate cancer (10 cases of highîGleason score prostate cancer ï¼»HGPCï¼½ and 10 cases of lowîGleason score prostate cancer ï¼»LGPCï¼½) and another 5 with benign prostatic hyperplasia (BPH). After transfection of H19 siRNA into the DU145 and PCî3 prostate cancer cells, the growth of the cells and the H19 expression in the cells were determined by MTT and qRTîPCR respectively, and the changes in the glycometabolism of the prostate cancer cells were analyzed by measuring the contents of glucose and lactate in the culture medium. Nonîtransfected and transfected negative vectors were used as blank and negative controls respectively. RESULTS: The relative expression of H19 was significantly increased in both the HGPC and LGPC tissues (0.725±0.385 and 2.086±0.542) as compared with that in the BPH tissue (0.210±0.068) (P< 0.01), even higher in the HGPC than in the LGPC tissue (P< 0.01). After transfection of H19 siRNA, the expressions of H19 were remarkably decreased in the DU145 and PCî3 prostate cancer cells in comparison with those in the blank control and negative control groups (P< 0.01), and so were the proliferation of and the glucose and lactate levels in the DU145 and PCî3 cells (P< 0.01).