FERM domain containing kindlin 1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLR family pyrin domain containing 3/nuclear factor kappa B pathway.

Intracerebral hemorrhage (ICH) is an incurable neurological disease. Microglia activation and its related inflammation contribute to ICH-associated brain damage. FERM domain containing kindlin 1 (FERMT1) is an integrin-binding protein that participates in microglia-associated inflammation, but its role in ICH is unclear. An ICH model was constructed by injecting 50 µl of autologous blood into the bregma of rats. FERMT1 siRNA was injected into the right ventricle of the rat for knockdown of FERMT1. A significant striatal hematoma was observed in ICH rats. FERMT1 knockdown reduced the water content of brain tissue, alleviated brain hematoma and improved behavioral function in ICH rats. FERMT1 knockdown reduced microglia activity, inhibited NLR family pyrin domain containing 3 (NLRP3) inflammasome activity and decreased the expression of inflammatory factors including IL-1ß and IL-18 in the peri-hematoma tissues. BV2 microglial cells were transfected with FERMT1 siRNA and incubated with 60 µM Hemin for 24 h. Activation of NLRP3 inflammasome induced by hemin were reduced in microglia when FERMT1 was knocked down, leading to decreased production of inflammatory factors IL-1ß and IL-18. In addition, knockdown of FERMT1 prevented the activation of nuclear factor kappa B (NF-κB) signaling pathway in vivo and in vitro. Our findings suggested that down-regulation of FERMT1 attenuated microglial inflammation and brain damage induced by ICH via NLRP3/NF-κB pathway. FERMT1 is a key regulator of inflammatory damage in rats after ICH.

Characterization of Calcium Silicate Hydrate Gels with Different Calcium to Silica Ratios and Polymer Modifications.

Calcium silicate hydrate (CSH) gels, the main binding phases of hydrated cement, are the most widely utilized synthetic materials. To understand the influences of composition and polymers on the reaction kinetics and phase formation, CSH gels with varying Ca/Si ratios and amounts of poly (acrylamide-co-acrylic acid) partial sodium salt (PAAm-co-PAA) were synthesized via a direct method. The CSH gels were characterized through isothermal calorimetry, thermogravimetric analysis (TGA), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Raman spectroscopy at different ages. By increasing the Ca/Si ratio from 0.8 to 1.0, the formation of CSH was enhanced with a 5.4% lower activation energy, whereas the incorporation of PAAm-co-PAA increased the temperature sensitivity of the reactions with an 83.3% higher activation energy. In the presence of PAAm-co-PAA, the reaction rate was retarded at an early age and the negative impact faded over time. The results of an XRD analysis indicated the formation of tobermorite as the main phase of the CSH gels, while the addition of PAAm-co-PAA resulted in a postponed calcium hydroxide consumption and CSH formation, which was confirmed by the decreased FTIR intensity of the C=O bond, Si-O stretching and Si-O bonds. The increased Raman vibrations of Si-O-Si bending Q2, Ca-O bonds, O-Si-O and asymmetric bending vibrations of SiO4 tetrahedra in the presence of PAAm-co-PAA indicate the intercalation of the polymeric phase and internal deformation of CSH gels.

Artificial Intelligence Algorithm-Based Intraoperative Magnetic Resonance Navigation for Glioma Resection.

The study aimed to analyze the application value of artificial intelligence algorithm-based intraoperative magnetic resonance imaging (iMRI) in neurosurgical glioma resection. 108 patients with glioma in a hospital were selected and divided into the experimental group (intraoperative magnetic resonance assisted glioma resection) and the control group (conventional surgical experience resection), with 54 patients in each group. After the resection, the tumor resection rate, NIHSS (National Institute of Health Stroke Scale) score, Karnofsky score, and postoperative intracranial infection were calculated in the two groups. The results revealed that the average tumor resection rate in the experimental group was significantly higher than that in the control group (P < 0.05). There was no significant difference in Karnofsky score before and after the operation in the experimental group (P > 0.05). There was no significant difference in NIHSS score between the experimental group and the control group after resection (P > 0.05). The number of patients with postoperative neurological deficits in the experimental group was smaller than that in the control group. In addition, there was no significant difference in infection rates between the two groups after glioma resection (P > 0.05). In summary, intraoperative magnetic resonance navigation on the basis of a segmentation dictionary learning algorithm has great clinical value in neurosurgical glioma resection. It can maximize the removal of tumors and ensure the integrity of neurological function while avoiding an increased risk of postoperative infection, which is of great significance for the treatment of glioma.

Changes in CA15-3, S100B, and IGF-1 in glioma and their predictive value for treatment efficacy.

OBJECTIVE: To analyze the changes of carbohydrate antigen 153 (CA15-3), S-100 calcium-binding protein B (S100B) and insulin-like growth factor-1 (IGF-1) in the treatment of patients with high-grade glioma and their predictive value for efficacy. METHODS: In this retrospective the PG and CG study, 74 patients with glioma who were treated in the Affiliated Hospital of Yan'an University from January 2015 to January 2017 were labeled as the patient group (PG); the other 70 patients who underwent craniocerebral trauma surgery during the same period were selected as the control group (CG). The expressions of CA15-3, S100B and IGF-1 in the PG and CG were compared. The relationship between CA15-3, S100B, IGF-1, and the pathologic data of patients was analyzed. The expression differences of CA15-3, S100B, and IGF-1 were compared between low-grade glioma patients and high-grade glioma patients and their diagnostic value was analyzed. The values of CA15-3, S100B, and IGF-1 expression for predicting treatment efficacy were analyzed. RESULTS: Expressions of CA15-3, S100B, and IGF-1 in glioma patients were markedly higher than those in the CG (P<0.0001). The proportion of grade III+IV patients with high expression of CA15-3, S100B, and IGF-1 was higher than in grade II patients (P<0.05), and the expressions of CA15-3, S100B and IGF-1 in low-grade glioma patients were lower than in high-grade glioma (P<0.01). The AUCs of CA15-3, S100B, and IGF-1 in differentiating different grades of glioma were 0.822, 0.722, and 0.768, respectively. Serum CA15-3, S100B and IGF-1 levels of the patients after treatment were significantly lower than those before treatment (P<0.0001). With the deterioration of clinical efficacy, serum levels of CA15-3, S100B, and IGF-1 gradually increased (P<0.05), and CA15-3, S100B and IGF-1 were positively correlated with therapeutic efficacy (P<0.05). AUCs of CA15-3, S100B, and IGF-1 for predicting the clinical efficacy in glioma patients were 0.824, 0.741, and 0.800, respectively. CONCLUSION: CA15-3, S100B, and IGF-1 are highly expressed in patients with glioma. They are diagnostic indicators to distinguish patients with high-grade glioma, and have predictive value for treatment efficacy.

Feasibility and Compatibility of a Biomass Capsule System in Self-Healing Concrete.

Cracking can facilitate deteriorations of concrete structures via various mechanisms by providing ingress pathways for moisture and aggressive chemicals. In contrast to conventional maintenance methods, self-healing is a promising strategy for achieving automatic crack repair without human intervention. However, in capsule-based self-healing concrete, the dilemma between capsules' survivability and crack healing efficiency is still an unfathomed challenge. In this study, the feasibility of a novel property-switchable capsule system based on a sustainable biomass component, polylactic acid, is investigated. Capsules with different geometries and dimensions were studied focusing on the compatibility with concrete, including survivability during concrete mixing, influence on mortar and concrete properties, and property evolution of the capsules. The results indicate that the developed elliptical capsules can survive regular concrete mixing with a survival ratio of 95%. In concrete containing 5 vol.% of gravel-level capsules, the compressive strength was decreased by 13.5% after 90 days, while the tensile strength was increased by 4.8%. The incorporation of 2 vol.% of sand-level capsules did not impact the mortar strength. Degradation and switchable properties triggered by the alkaline matrix of cement were observed, revealing the potential of this novel biomass capsule system in achieving both high survivability and self-healing efficiency in concrete.

A Computational Study of the Shear Behavior of Reinforced Concrete Beams Affected from Alkali-Silica Reactivity Damage.

In this study, an investigation of the shear behavior of full-scale reinforced concrete (RC) beams affected from alkali-silica reactivity damage is presented. A detailed finite element model (FEM) was developed and validated with data obtained from the experiments using several metrics, including a force-deformation curve, rebar strains, and crack maps and width. The validated FEM was used in a parametric study to investigate the potential impact of alkali-silica reactivity (ASR) degradation on the shear capacity of the beam. Degradations of concrete mechanical properties were correlated with ASR expansion using material test data and implemented in the FEM for different expansions. The finite element (FE) analysis provided a better understanding of the failure mechanism of ASR-affected RC beam and degradation in the capacity as a function of the ASR expansion. The parametric study using the FEM showed 6%, 19%, and 25% reduction in the shear capacity of the beam, respectively, affected from 0.2%, 0.4%, and 0.6% of ASR-induced expansion.

Impact of Laboratory-Accelerated Aging Methods to Study Alkali-Silica Reaction and Reinforcement Corrosion on the Properties of Concrete.

This study focuses on two separate investigations of the main aging mechanisms: alkali-silica reactivity (ASR) and the corrosion of reinforcing steel (rebar) concrete, both of which may result in a premature failure to meet the serviceability or strength requirements of a concrete structure. However, these processes occur very slowly, spanning decades. The impact of direct chemical additives to fresh concrete to accelerate ASR and the corrosion of reinforcing steel on the fresh and hardened properties of the ensuing material are investigated to inform the potential use of chemicals in large-scale studies. The deterioration of reinforced concrete (RC) is determined by means of expansion, cracking, bulk diffusivity and surface resistivity measurements, and compressive, split tensile and flexural strength tests. The results indicate that the addition of sodium hydroxide and calcium chloride can effectively accelerate the crack formation and propagation in concrete due to ASR and the corrosion of rebar, respectively. The ASR-induced cracks maintained a constant crack width from 0.05 mm to 0.1 mm over the measurement period regardless of the intensity of aging acceleration. Adding 4% chloride by weight of cement for accelerating rebar corrosion resulted in an average crack that was 82% larger than in the case of ASR accelerated with the addition of sodium hydroxide. The addition of alkali resulted in an increase in early-age (7-day) strength. At a total alkali loading of 2.98 kg/m3, 3.84 kg/m3 and 5.57 kg/m3, the 28-day compressive strength of concrete decreased by 3%, 10% and 24%, respectively. Similarly, a higher early-age strength and a lower later-age strength was observed for the concrete in the presence of corrosive calcium chloride. The results from this research are expected to inform future studies on the long-term performance of RC structures under accelerated ASR and corrosion.

Caspase activity and oxidative stress of granulosa cells are associated with the viability and developmental potential of vitrified immature oocytes.

OBJECTIVE: The aim of this study is to determine whether caspase activity and oxidative stress of granulosa cells are associated with the viability and developmental potential of vitrified immature oocytes. STUDY DESIGN: Oocytes from mice were exposed to genistein or/and Z-VAD-FMK with or without vitrification. Ultrastructural alterations of granulosa cells in vitrified immature oocytes were observed. Moreover, the level of superoxide dismutase (SOD) in granulosa cells, incidence of apoptotic follicles, the viability of vitrified-warmed oocytes and their subsequent developmental competence were measured. RESULTS: Ultrastructural alterations of granulosa cells vitrified in the presence of genistein or Z-VAD-FMK were slighter than that of granulosa cells vitrified in the absence of genistein or Z-VAD-FMK. The incidence of apoptotic follicles vitrified in the presence of genistein or Z-VAD-FMK was significantly lower than that of immature oocytes vitrified in the absence of genistein or Z-VAD-FMK, whereas, the level of SOD in granulosa cells, the viability and developmental competence of immature oocytes vitrified in the presence of genistein or Z-VAD-FMK were significantly higher than that of immature oocytes vitrified in the absence of genistein or Z-VAD-FMK. CONCLUSION: Both antioxidant (genistein) and caspase inhibition (Z-VAD-FMK) improve the viability and developmental competence of vitrified immature oocytes. Genistein is superior to Z-VAD-FMK in improving the efficacy of immature oocyte vitrification.

Pancreatitis in hand-foot-and-mouth disease caused by enterovirus 71.

Some viruses, including certain members of the enterovirus genus, have been reported to cause pancreatitis, especially Coxsackie virus. However, no case of human enterovirus 71 (EV71) associated with pancreatitis has been reported so far. We here report a case of EV71-induced hand-foot-and-mouth disease (HFMD) presenting with pancreatitis in a 2-year-old girl. This is the first report of a patient with acute pancreatitis in HFMD caused by EV71. We treated the patient conservatively with nasogastric suction, intravenous fluid and antivirals. The patient's symptoms improved after 8 d, and recovered without complications. We conclude that EV71 can cause acute pancreatitis in HFMD, which should be considered in differential diagnosis, especially in cases of idiopathic pancreatitis.

Attenuation of brain edema and spatial learning deficits by the inhibition of NADPH oxidase activity using apocynin following diffuse traumatic brain injury in rats.

Diffuse brain injury (DBI) is a leading cause of mortality and disability among young individuals and adults worldwide. In specific cases, DBI is associated with permanent spatial learning dysfunction and motor deficits due to primary and secondary brain damage. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) is a major complex that produces reactive oxygen species (ROS) during the ischemic period. The complex aggravates brain damage and cell death following ischemia/reperfusion injury; however, its role in DBI remains unclear. The present study aimed to investigate the hypothesis that levels of NOX2 (a catalytic subunit of NOX) protein expression and the activation of NOX are enhanced following DBI induction in rats and are involved in aggravating secondary brain damage. A rat model of DBI was created using a modified weight-drop device. Our results demonstrated that NOX2 protein expression and NOX activity were enhanced in the CA1 subfield of the hippocampus at 48 and 72 h following DBI induction. Treatment with apocynin (50 mg/kg body weight), a specific inhibitor of NOX, injected intraperitoneally 30 min prior to DBI significantly attenuated NOX2 protein expression and NOX activation. Moreover, treatment with apocynin reduced brain edema and improved spatial learning function assessed using the Morris water maze. These results reveal that treatment with apocynin may provide a new neuroprotective therapeutic strategy against DBI by diminishing the upregulation of NOX2 protein and NOX activity.