Evaluation of indicators of acute emotional states in dogs.

A complete assessment of animal welfare requires not just an understanding of negative emotional states, such as fear and anxiety, but also of positive states, such as calmness and happiness. However, few studies have identified accurate and reliable indicators of positive emotional states in dogs. This study aimed to identify parameters that may serve as indicators of short-term emotional states in dogs. Using a cross-over design, 60 dogs living at a research facility were exposed to six different 10-min scenarios expected to elicit responses varying in emotional valence and arousal. A range of behavioural and physiological parameters were collected and their relationship to anticipated emotional valence and arousal was analysed using linear and logistic mixed models. Cortisol, adrenocorticotropic hormone, heart rate variability, panting, whining, and body shake all demonstrated significant differences based on arousal levels, but only within negative valence scenarios. Scores from a qualitative behavioural assessment (QBA) were associated with both emotional valence and arousal and were considered the best indicator of positive valence. Activity, ear temperature, and sitting were associated with positive high arousal, although this may have been influenced by differing levels of movement induced during these scenarios. Meanwhile, heart rate, secretory immunoglobulin A, standing and lying all showed similar changes associated with arousal for both positive and negative valence scenarios. This study provides a critical first step towards identifying evidence-based indicators of short-term emotional states in dogs, while highlighting considerations that should be made when employing these parameters, including the influence of coder bias, food provision, exercise, and external temperature. Overall, it is recommended future dog emotion and welfare research use a combination of parameters including indicators of both emotional valence and arousal.

Dam (Canis familiaris) Welfare throughout the Peri-Parturient Period in Commercial Breeding Kennels.

Poor dam welfare throughout the peri-parturient period can also negatively affect that of their offspring. This study aimed to identify changes in physical, physiological, and behavioral metrics indicative of dam welfare throughout the peri-parturient period. Dams (n = 74) from eight U.S. Midwest commercial breeding (CB) kennels were tested at 6 and 1 week prepartum, and 4 and 8 weeks postpartum. At each time point dams underwent a stranger approach test, physical health assessment, hair collection for hair cortisol concentration (HCC) and fecal collection for fecal glucocorticoid metabolites (FGM), fecal secretory immunoglobulin A (sIgA) and parasite detection. Linear mixed-effects models indicated dams exhibited more affiliative behaviors towards the stranger at 4 weeks postpartum than 6 weeks prepartum (p = 0.03), increased HCC from 4-weeks to 8 weeks postpartum (p = 0.02), and increased FGM from 1 week prepartum to 8 weeks postpartum (p = 0.04). At each respective time point, the percentage of dams with intestinal parasites was 11%, 4%, 23%, and 15%. Most changes are likely due to increased energy requirements and hormonal variations. However, deviations from expected changes may have resulted from changes in environment and/ or management, which should be explored in future studies.

The Effect of Transportation on Puppy Welfare from Commercial Breeding Kennels to a Distributor.

Many puppies from commercial breeding kennels (CBKs) are transported by ground from their kennels of origin to a distributor. This experience may elicit fear and stress during a sensitive developmental period, which may in turn negatively impact the puppies' short- and long-term welfare. This study aimed to measure short-term effects of transportation on puppy welfare metrics. Eight-week-old puppies (n = 383) from 12 CBKs were tested at their kennels (pre-trans) and ~48 h after arriving at a distributor (post-trans). At each location, puppies underwent an isolation test, a stranger-approach test, and a physical health assessment. Behavioral responses to testing were scored from videos. Fecal glucocorticoid metabolites (FGM), fecal secretory immunoglobulin A (sIgA), and presence of intestinal parasites were also analyzed. Linear mixed-effects models identified decreased exploration (p < 0.001), and increased locomotion (p < 0.001) and escape attempts (p = 0.001) during the post-trans isolation test. Increased affiliative behavior (p < 0.001), FGM (p < 0.001) and sIgA (p = 0.014) were also observed post-trans. Findings support good physical health both pre- and post-trans, while behavioral and physiological changes suggest increased puppy distress post-trans. Higher post-transport affiliative behavior may indicate that puppies sought social support as a coping strategy after experiencing transport-related distress. Future studies should explore the efficacy of transportation-related interventions to mitigate puppy distress.

The influence of early life socialisation on cognition in the domestic pig (Sus scrofa domestica).

Previously, the benefits of early-life socialisation on later-life social development have been reported in pigs. Here we investigated the effect of pre-weaning socialisation on the later-life cognitive ability of pigs using a range of techniques. Pre-weaning, 101 piglets had access to a neighbouring pen from ~ 15 days of age and interacted with non-littermates (socialised). An additional 89 piglets remained isolated within their home pen (controls). After weaning, 100 individuals were selected for a range of cognitive tests including a food reward T-maze test, reversal learning T-maze test, a social preference T-maze test, and a puzzle box test. Performance during the food reward test was not influenced by treatment. Treatment effected improvement over the course of the reversal learning test, with controls showing a significant decrease in trial duration after the first two trials. During the social preference test, socialised pigs spent significantly more time in the presence of larger stimulus pigs than controls and were quicker to leave the middle of the maze, suggesting improved social skills. Neither sex nor treatment was observed to influence pig's ability to solve the puzzle box. Thus, overall, evidence from the social preference test suggests an effect of pre-weaning socialisation on aspects of social cognitive development.

The Association Between Play Fighting and Information Gathering during Subsequent Contests.

Many hypotheses regarding the evolution of social play have been suggested, including the development of later-life assessment skills. However, the link between play fighting experience and information gathering during contests has yet to be examined. This paper explores the association between play fighting and contest assessment strategy in the domestic pig (Sus scrofa). Using an established framework, we provide evidence suggesting play fighting frequency may affect the extent to which individuals incorporate information regarding their own and their competitors' resource holding potential (RHP) in escalation decisions. Pigs were allocated as 'high play' or 'low play' based upon their relative play fighting frequency. To maximise variation in play, 12 litters underwent a socialisation treatment while the remaining 12 litters were kept isolated within their home pen (i.e. control treatment). At eight weeks of age contests were staged between pairs of unfamiliar pigs, using 19 'high play' dyads and 19 'low play' dyads. While 'high play' dyads were observed to rely on a pure self-assessment strategy, 'low play dyads' did not meet the predictions of either self- or mutual assessment, suggesting their contest behaviour may have been motivated by alternative factors. We suggest that early life play fighting may therefore allow individuals to develop an accurate estimate of their RHP.

Play fighting social networks do not predict injuries from later aggression.

Early play fighting mimics later aggression in many species, and may, therefore, be expected to reduce costs from later aggressive interactions. Using social network analysis (SNA) the effect of a central play fighting network position on later skin lesions from aggression was assessed in domestic pigs. Piglets (n = 263) were kept in litter groups or socialised pre-weaning with another litter to enhance play fighting experience. Play fighting was recorded for 1.5 h per day over 6 days pre-weaning. Play fighting network centrality was quantified using measures of individual network position and entire network structure (degree, eigenvector, betweenness, clustering coefficient). Skin lesions from aggression were counted after a dyadic contest and at 24 h and 3 weeks following group mixing. Pigs with play fighting interactions with many partners experienced fewer lesions from the dyadic contest (in-degree, p = 0.01) and tended to received fewer lesions 3 weeks after group mixing (degree, p = 0.088) but no other play fighting centrality measures affected the number of lesions at any point. The benefits of play fighting were therefore limited to specific aggressive social contexts. The tendency of socialised piglets to play fight with non-littermates did not affect subsequent lesions. We advocate the use of SNA over approaches that only consider dyadic interactions to further our understanding of the influence of early social group interactions on later life experience.

Socialisation and its effect on play behaviour and aggression in the domestic pig (Sus scrofa).

There is considerable interest in how early life experiences shape behavioural development. For example, the socialisation of unfamiliar pigs pre-weaning has been suggested to decrease aggression during later life. However, the behavioural mechanisms behind this socialisation effect remain unexplored. We allowed 12 litters of domestic pigs (Sus scrofa) to move freely between their home pen and a neighbouring pen (socialisation) during the lactation period, while keeping 12 litters isolated in their home pen (control). Contrary to predictions, socialisation did not result in higher levels of social play. However, control individuals engaged in more sow directed play than those that underwent socialisation. Consistent with predictions, males performed more piglet directed play than females. Social play behaviour pre-weaning was found to be highly concordant within individuals from both treatments. Post-weaning, 148 pigs were selected to perform two resident-intruder tests to assay aggressiveness. As predicted, socialised individuals were quicker to attack than controls, although females were more aggressive than males. Additionally, play fighting experience was found to negatively correlate with attack latency in females, supporting the hypothesis that early-life play experience is likely to be sexually dimorphic when males and females show pronounced differences in their later-life social behaviour.