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To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process previously described to be cell autonomous. We have discovered that, in Caenorhabditis elegans, neuronal heat shock factor 1 (HSF-1), the conserved master regulator of the heat shock response (HSR), causes extensive fat remodeling in peripheral tissues. These changes include a decrease in fat desaturase and acid lipase expression in the intestine and a global shift in the saturation levels of plasma membrane's phospholipids. The observed remodeling of plasma membrane is in line with ectothermic adaptive responses and gives worms a cumulative advantage to warm temperatures. We have determined that at least 6 TAX-2/TAX-4 cyclic guanosine monophosphate (cGMP) gated channel expressing sensory neurons, and transforming growth factor ß (TGF-ß)/bone morphogenetic protein (BMP) are required for signaling across tissues to modulate fat desaturation. We also find neuronal hsf-1 is not only sufficient but also partially necessary to control the fat remodeling response and for survival at warm temperatures. This is the first study to show that a thermostat-based mechanism can cell nonautonomously coordinate membrane saturation and composition across tissues in a multicellular animal.
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Adaptação Fisiológica , Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/fisiologia , Temperatura Alta , Lipídeos/química , Neurônios/metabolismo , Fatores de Transcrição/metabolismo , Animais , Proteínas Morfogenéticas Ósseas/metabolismo , Caenorhabditis elegans/genética , Caenorhabditis elegans/crescimento & desenvolvimento , Temperatura Baixa , GMP Cíclico/metabolismo , Glicerofosfolipídeos/metabolismo , Fenótipo , Transdução de Sinais , Estresse Fisiológico , Transcrição Gênica , Fator de Crescimento Transformador beta/metabolismoRESUMO
Humans show a remarkable capacity to navigate various environments using different navigation strategies, and we know that strategy changes across the life span. However, this observation has been based on studies of small sample sizes. To this end, we used a mobile app-based video game (Sea Hero Quest) to test virtual navigation strategies and memory performance within a distinct radial arm maze level in over 37,000 participants. Players were presented with six pathways (three open and three closed) and were required to navigate to the three open pathways to collect a target. Next, all six pathways were made available and the player was required to visit the pathways that were previously unavailable. Both reference memory and working memory errors were calculated. Crucially, at the end of the level, the player was asked a multiple-choice question about how they found the targets (i.e., a counting-dependent strategy vs. a landmark-dependent strategy). As predicted from previous laboratory studies, we found the use of landmarks declined linearly with age. Those using landmark-based strategies also performed better on reference memory than those using a counting-based strategy. These results extend previous observations in the laboratory showing a decreased use of landmark-dependent strategies with age.
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Navegação Espacial , Jogos de Vídeo , Humanos , Longevidade , Percepção Espacial , Memória de Curto PrazoRESUMO
BACKGROUND: The extent to which digital media use by adolescents contributes to poor mental health, or vice-versa, remains unclear. The purpose of the present study is to clarify the strength and direction of associations between adolescent internet use and the development of depression symptoms using a longitudinal modeling approach. We also examine whether associations differ for boys and girls. METHODS: Data are drawn from (N = 1547) participants followed for the Quebec longitudinal Study of Child Development (QLSCD 1998-2020). Youth self-reported internet use in terms of the average hours of use per week at the ages of 13, 15, and 17. Youth also self-reported depression symptoms at the same ages. RESULTS: After testing sex-invariance, random intercepts cross-lagged panel models stratified by sex, revealed that internet use by girls was associated with significant within-person (time-varying) change in depression symptoms. Girl's internet use at age 13 was associated with increased depression symptoms at age 15 (ß = 0.12) and internet use at age 15 increased depression at age 17 (ß = 0.10). For boys, internet use was not associated with significant time varying change in depression symptoms. CONCLUSIONS: The present findings support the hypothesis that internet use by adolescents can represent a significant risk factor for the development of depressive symptoms, particularly in girls.
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Extraction protocols and liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry (LC-MS/MS) methods for the measurement of four lipid categories, namely glycerophospholipids, glycerolipids, sphingolipids and sterol lipids in human plasma, are described here. Step-by-step instructions are provided for the liquid-liquid extraction methods, including solution preparation and the non-endogenous lipid internal standards used. All instrumental conditions, chromatography columns and solutions required for the LC-MS and LC-MS/MS methods are also provided in detail.
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Lipidômica , Espectrometria de Massas em Tandem , Humanos , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida/métodos , Esfingolipídeos/análise , Espectrometria de Massa com Cromatografia LíquidaRESUMO
RATIONALE: The present work shows comprehensive chromatographic methods and MS conditions that have been developed based on the chemical properties of each lipid subclass to detect low-abundance molecular species. This study shows that the developed methods can detect low- and/or very-low-abundant lipids like phosphatidic acid (PA) in the glycerophospholipid (GP) method; dihydroceramide (dhCer) and dihydrosphingosine/sphinganine (dhSPB) in the sphingolipid (SP) method; and lysophosphatidic acid (LPA), LPI, LPG and sphingosine-1-phosphate (SPBP) in the lysolipid method. METHODS: An optimised method for the extraction of lysolipids in plasma is used in addition to Folch extraction. Then, four chromatographic methods coupled with mass spectrometry using targeted and untargeted approaches are described here. Three of the methods use a tertiary pumping system to enable the inclusion of a gradient for analyte separation (pumps A and B) and an isocratic wash (pump C). This wash solution elutes interfering compounds that could cause background signal in the subsequent injections, reducing column lifetime. RESULTS: Semi-quantitative values for 37 lipid subclasses are reported for a plasma sample (NIST SRM 1950). Furthermore, the methods presented here enabled the identification of 338 different lipid molecular species for GPs (mono- and diacyl-phospholipds), SPs, sterols and glycerolipids. The methods have been validated, and the reproducibility is presented here. CONCLUSIONS: The comprehensive analysis of the lipidome addressed here of glycerolipids, GPs, sterols and SPs is in good agreement with previously reported results, in the NIST SRM 1950 sample, by other laboratories. Ten lipid subclasses LPS, LPI, alkyl-lysophosphatidic acid/alkenyl-lysophosphatidic acid, alkyl-lysophosphatidylethanolamine/alkenyl-lysophosphatidylethanolamine, dhCer (d18:0), SPB (d18:1), dhSPB (d18:0) and SPBP (d18:2) have been detected using this comprehensive method and are uniquely reported here.
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People can navigate in a new environment using multiple strategies dependent on different memory systems. A series of studies have dissociated between hippocampus-dependent 'spatial' navigation and habit-based 'response' learning mediated by the caudate nucleus. The val66met polymorphism of the brain-derived neurotrophic factor (BDNF) gene leads to decreased secretion of BDNF in the brain, including the hippocampus. Here, we aim to investigate the role of the BDNF val66met polymorphism on virtual navigation behaviour and brain activity in healthy older adults. A total of 139 healthy older adult participants (mean age = 65.8 ± 4.4 years) were tested in this study. Blood samples were collected, and BDNF val66met genotyping was performed. Participants were divided into two genotype groups: val homozygotes and met carriers. Participants were tested on virtual dual-solution navigation tasks in which they could use either a hippocampus-dependent spatial strategy or a caudate nucleus-dependent response strategy to solve the task. A subset of the participants (n = 66) were then scanned in a 3T functional magnetic resonance imaging (fMRI) scanner while engaging in another dual-solution navigation task. BDNF val/val individuals and met carriers did not differ in learning performance. However, the two BDNF groups differed in learning strategy. BDNF val/val individuals relied more on landmarks to remember target locations (i.e., increased use of flexible spatial learning), while met carriers relied more on sequences and patterns to remember target locations (i.e., increased use of inflexible response learning). Additionally, BDNF val/val individuals had more fMRI activity in the hippocampus compared with BDNF met carriers during performance on the navigation task. This is the first study to show in older adults that BDNF met carriers use alternate learning strategies from val/val individuals and to identify differential brain activation of this behavioural difference between the two groups.
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Fator Neurotrófico Derivado do Encéfalo , Hipocampo , Navegação Espacial , Idoso , Fator Neurotrófico Derivado do Encéfalo/genética , Genótipo , Hipocampo/diagnóstico por imagem , Humanos , Imageamento por Ressonância Magnética , Pessoa de Meia-Idade , Polimorfismo Genético , Polimorfismo de Nucleotídeo ÚnicoRESUMO
Previous studies showed that healthy young adults who spontaneously use caudate nucleus-dependent strategies on a virtual navigation task, have significantly lower basal levels of cortisol compared with adults who use hippocampus-dependent spatial navigation strategies. In the current paper, we assessed the relation between basal cortisol levels and learning using a virtual navigation task in children. We show that basal cortisol level has a differential effect on learning and memory between children using spatial and response navigation strategies. Specifically, cortisol was found to be beneficial for learning performance in children using spatial strategies, such that higher levels of cortisol were associated with more efficient learning in a virtual maze. In contrast, cortisol had a deleterious effect on learning the virtual maze in children using response strategies, such that higher cortisol levels were associated with increased spatial working memory errors. Based on these results, individual differences in navigation strategy could help explain contradictory results in the literature showing that cortisol can have either a positive or negative association with learning and memory performance.
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Hidrocortisona/fisiologia , Aprendizagem em Labirinto/fisiologia , Memória Espacial/fisiologia , Navegação Espacial/fisiologia , Núcleo Caudado/fisiologia , Criança , Feminino , Hipocampo/fisiologia , Humanos , Masculino , Memória de Curto Prazo/fisiologiaRESUMO
When people navigate, they use strategies dependent on one of two memory systems. The hippocampus-based spatial strategy consists of using multiple landmarks to create a cognitive map of the environment. In contrast, the caudate nucleus-based response strategy is based on the memorization of a series of turns. Importantly, response learners display more gray matter and functional activity in the caudate nucleus and less gray matter in the hippocampus. In parallel, the caudate nucleus is involved in decision-making by mediating attention toward rewards and in set-shifting by mediating preparatory actions. The present study, therefore, examined the link between navigational strategy use, that are associated with gray matter differences in the caudate nucleus and hippocampus, and decision-making and set-shifting performance. Fifty-three participants completed the 4 on 8 virtual maze, the Iowa Gambling Task (IGT), the Wisconsin Card Sorting Test-64 (WCST-64), and a task-switching test. The results revealed that people who use response strategies displayed increased risk-taking behavior in the IGT compared to the people using hippocampus-dependent spatial strategies. Response strategy was also associated with enhanced set-shifting performance in the WCST-64 and task-switching test. These results confirm that risk-taking and set-shifting behavior, that are differentially impacted by the caudate nucleus and hippocampus memory systems, can be predicted by navigational strategy.
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Núcleo Caudado/fisiologia , Tomada de Decisões/fisiologia , Memória/fisiologia , Assunção de Riscos , Navegação Espacial/fisiologia , Adolescente , Adulto , Feminino , Hipocampo/fisiologia , Humanos , Masculino , Adulto JovemRESUMO
Aging is associated with cognitive decline and decreased capacity to inhibit distracting information. Video game training holds promise to increase inhibitory mechanisms in older adults. In the current study, we tested the impact of 3D-platform video game training on performance in an antisaccade task and on related changes in grey matter within the frontal eye fields (FEFs) of older adults. An experimental group (VID group) engaged in 3D-platform video game training over a period of 6 months, while an active control group was trained on piano lessons (MUS group), and a no-contact control group did not participate in any intervention (CON group). Increased performance in oculomotor inhibition, as measured by the antisaccade task, and increased grey matter in the right FEF was observed uniquely in the VID group. These results demonstrate that 3D-platform video game training can improve inhibitory control known to decline with age.
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Envelhecimento/fisiologia , Atenção/fisiologia , Lobo Frontal/fisiologia , Substância Cinzenta/fisiologia , Inibição Psicológica , Prática Psicológica , Desempenho Psicomotor/fisiologia , Movimentos Sacádicos/fisiologia , Jogos de Vídeo , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Experienced musicians outperform non-musicians in understanding speech-in-noise (SPIN). The benefits of lifelong musicianship endure into older age, where musicians experience smaller declines in their ability to understand speech in noisy environments. However, it is presently unknown whether commencing musical training in old age can also counteract age-related decline in speech perception, and whether such training induces changes in neural processing of speech. Here, we recruited older adult non-musicians and assigned them to receive a short course of piano or videogame training, or no training. Participants completed two sessions of functional Magnetic Resonance Imaging where they performed a SPIN task prior to and following training. While we found no direct benefit of musical training upon SPIN perception, an exploratory Region of Interest analysis revealed increased cortical responses to speech in left Middle Frontal and Supramarginal Gyri which correlated with changes in SPIN task performance in the group which received music training. These results suggest that short-term musical training in older adults may enhance neural encoding of speech, with the potential to reduce age-related decline in speech perception.
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Música , Prática Psicológica , Percepção da Fala/fisiologia , Fala , Idoso , Feminino , Audição , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Ruído , Resultado do TratamentoRESUMO
One of two memory systems can be used to navigate in a new environment. Hippocampus-dependent spatial strategy consists of creating a cognitive map of an environment and caudate nucleus-dependent response strategy consists of memorizing a rigid sequence of turns. Spontaneous use of the response strategy is associated with greater activity and grey matter within the caudate nucleus while the spatial strategy is associated with greater activity and grey matter in the hippocampus. The caudate nucleus is involved in executive functions such as working memory, cognitive control and certain aspects of attention such as attentional disengaging. This study therefore aimed to investigate whether response learners would display better performance on tests of executive and attention functioning compared to spatial learners. Fifty participants completed the 4/8 virtual maze to assess navigational strategy, the forward and backward visual digit span and the Attention Network Test - Revised to assess both attention disengagement and cognitive control. Results revealed that response learners showed significantly higher working memory capacity, more efficient attention disengagement and better cognitive control. Results suggest that response learners, who putatively display more grey matter and activity in the caudate nucleus, are associated with better working memory span, cognitive control and attentional disengagement.
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Atenção/fisiologia , Núcleo Caudado/fisiologia , Memória de Curto Prazo/fisiologia , Aprendizagem Espacial/fisiologia , Navegação Espacial/fisiologia , Adolescente , Adulto , Cognição/fisiologia , Função Executiva/fisiologia , Feminino , Humanos , Masculino , Testes Neuropsicológicos , Adulto JovemRESUMO
Visual spatial attention is important during navigation processes that rely on a cognitive map, because spatial relationships between environmental landmarks need to be selected, encoded, and learned. People who navigate using this strategy are spatial learners, and this process relies on the hippocampus. Conversely, response learners memorize a series of actions to navigate, which relies on the caudate nucleus. Response learning, which is more efficient, is thought to involve less demanding cognitive operations, and is related to reduced grey matter in the hippocampus. To test if navigational strategy can impact visual attention performance, we investigated if spatial and response learners showed differences in attentional engagement used during a visual spatial task. We tested 40 response learners and 39 spatial learners, as determined by the 4-on-8 Virtual Maze (4/8 VM), on a target detection task designed to elicit an N2pc component (an index visual spatial attention). Spatial learners produced a larger N2pc amplitude during target detection compared to response learners. This relationship might represent an increase in goal-directed attention towards target stimuli or a more global increase in cognitive function that has been previously observed in spatial learners.
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Atenção/fisiologia , Potenciais Evocados/fisiologia , Percepção Espacial/fisiologia , Aprendizagem Espacial/fisiologia , Adulto , Mapeamento Encefálico , Eletroencefalografia , Feminino , Objetivos , Humanos , Masculino , Aprendizagem em Labirinto , Estimulação Luminosa , Interface Usuário-Computador , Adulto JovemRESUMO
The habitual playing of video games is associated with increased grey matter and activity in the striatum. Studies in humans and rodents have shown an inverse relationship between grey matter in the striatum and hippocampus. We investigated whether action video game playing is also associated with increased use of response learning strategies during navigation, known to be dependent on the caudate nucleus of the striatum, when presented in a dual solution task. We tested 26 action video game players (actionVGPs) and 33 non-action video game players (nonVGPs) on the 4-on-8 virtual maze and a visual attention event-related potential (ERP) task, which elicits a robust N-2-posterior-controlateral (N2pc) component. We found that actionVGPs had a significantly higher likelihood of using a response learning strategy (80.76%) compared to nonVGPs (42.42%). Consistent with previous evidence, actionVGPs and nonVGPs differed in the way they deployed visual attention to central and peripheral targets as observed in the elicited N2pc component during an ERP visual attention task. Increased use of the response strategy in actionVGPs is consistent with previously observed increases in striatal volume in video game players (VGPs). Using response strategies is associated with decreased grey matter in the hippocampus. Previous studies have shown that decreased volume in the hippocampus precedes the onset of many neurological and psychiatric disorders. If actionVGPs have lower grey matter in the hippocampus, as response learners normally do, then these individuals could be at increased risk of developing neurological and psychiatric disorders during their lifetime.
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Núcleo Caudado/fisiologia , Desempenho Psicomotor , Navegação Espacial , Jogos de Vídeo , Adulto , Feminino , Humanos , Masculino , Jogos de Vídeo/estatística & dados numéricos , Percepção Visual , Adulto JovemRESUMO
Coastal ecosystems are becoming increasingly threatened by human activities and there is growing appreciation that management must consider the impacts of multiple stressors. Cumulative effects assessments (CEAs) have become a popular tool for identifying the distribution and intensity of multiple human stressors in coastal ecosystems. Few studies, however, have demonstrated strong correlations between CEAs and change in ecosystem condition, questioning its management use. Here, we apply a CEA to the endangered seagrass Posidonia australis in Pittwater, NSW, Australia, using spatial data on known stressors to seagrass related to foreshore development, water quality, vessel traffic and fishing. We tested how well cumulative effects scores explained changes in P. australis extent measured between 2005 and 2019 using high-resolution aerial imagery. A negative correlation between P. australis and estimated cumulative effects scores was observed (R2 = 22 %), and we identified a threshold of cumulative effects above which losses of P. australis became more likely. Using baited remote underwater video, we surveyed fishes over P. australis and non-vegetated sediments to infer and quantify how impacts of cumulative effects to P. australis extent would flow on to fish assemblages. P. australis contained a distinct assemblage of fish, and on non-vegetated sediments the abundance of sparids, which are of importance to fisheries, increased with closer proximity to P. australis. Our results demonstrate the negative impact of multiple stressors on P. australis and the consequences for fish biodiversity and fisheries production across much of the estuary. Management actions aimed at reducing or limiting cumulative effects to low and moderate levels will help conserve P. australis and its associated fish biodiversity and productivity.
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Biodiversidade , Ecossistema , Animais , Humanos , Austrália , Estuários , Qualidade da Água , PeixesRESUMO
In late June 2021 a heatwave of unprecedented magnitude impacted the Pacific Northwest region of Canada and the United States. Many locations broke all-time maximum temperature records by more than 5 °C, and the Canadian national temperature record was broken by 4.6 °C, with a new record temperature of 49.6 °C. Here, we provide a comprehensive summary of this event and its impacts. Upstream diabatic heating played a key role in the magnitude of this anomaly. Weather forecasts provided advanced notice of the event, while sub-seasonal forecasts showed an increased likelihood of a heat extreme with lead times of 10-20 days. The impacts of this event were catastrophic, including hundreds of attributable deaths across the Pacific Northwest, mass-mortalities of marine life, reduced crop and fruit yields, river flooding from rapid snow and glacier melt, and a substantial increase in wildfires-the latter contributing to landslides in the months following. These impacts provide examples we can learn from and a vivid depiction of how climate change can be so devastating.
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OBJECTIVE: We aim to investigate the direction of causality of the association between adolescent video game playing and later development of ADHD symptoms using a population-based sample of Canadian Youth. METHOD: The present study is based on longitudinal cohort data (N = 1,467). Youth self-reported weekly hours of video game playing as well as ADHD symptoms at both 12 and 13 years of age. RESULTS: Cross-lagged panel model were estimated to examine how adolescent video game playing prospectively contributes to ADHD symptoms while simultaneously considering how adolescent ADHD symptoms may prospectively contribute to videogame playing. Analyses revealed a significant positive association between adolescent video games playing at age 12 and ADHD symptoms at age 13. Youth ADHD symptoms at age 12 did not predict video game use at age 13. CONCLUSION: Our results help clarify the direction of causality of the association between video game playing and ADHD symptoms and provide evidence that video game playing can represent a risk factor for the development of attention problems in early adolescence.
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Transtorno do Deficit de Atenção com Hiperatividade , Jogos de Vídeo , Adolescente , Transtorno do Deficit de Atenção com Hiperatividade/diagnóstico , Transtorno do Deficit de Atenção com Hiperatividade/epidemiologia , Canadá/epidemiologia , Criança , Humanos , Fatores de Risco , AutorrelatoRESUMO
When multiple stimuli are concurrently displayed in the visual field, they must compete for neural representation at the processing expense of their contemporaries. This biased competition is thought to begin as early as primary visual cortex, and can be driven by salient low-level stimulus features. Stimuli important for an organism's survival, such as facial expressions signaling environmental threat, might be similarly prioritized at this early stage of visual processing. In the present study, we used ERP recordings from striate cortex to examine whether fear expressions can bias the competition for neural representation at the earliest stage of retinotopic visuo-cortical processing when in direct competition with concurrently presented visual information of neutral valence. We found that within 50 msec after stimulus onset, information processing in primary visual cortex is biased in favor of perceptual representations of fear at the expense of competing visual information (Experiment 1). Additional experiments confirmed that the facial display's emotional content rather than low-level features is responsible for this prioritization in V1 (Experiment 2), and that this competition is reliant on a face's upright canonical orientation (Experiment 3). These results suggest that complex stimuli important for an organism's survival can indeed be prioritized at the earliest stage of cortical processing at the expense of competing information, with competition possibly beginning before encoding in V1.
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Atenção/fisiologia , Viés , Potenciais Evocados Visuais/fisiologia , Expressão Facial , Medo , Córtex Visual/fisiologia , Mapeamento Encefálico , Eletroencefalografia , Feminino , Humanos , Masculino , Estimulação Luminosa , Estudantes , UniversidadesRESUMO
According to evolutionary accounts, emotions originated to prepare an organism for action (Darwin 1872; Frijda 1986). To investigate this putative relationship between emotion and action, we examined the effect of an emotional stimulus on oculomotor actions controlled by the superior colliculus (SC), which has connections with subcortical structures involved in the perceptual prioritization of emotion, such as the amygdala through the pulvinar. The pulvinar connects the amygdala to cells in the SC responsible for the speed of saccade execution, while not affecting the spatial component of the saccade. We tested the effect of emotion on both temporal and spatial signatures of oculomotor functioning using a gap-distractor paradigm. Changes in spatial programming were examined through saccadic curvature in response to a remote distractor stimulus, while changes in temporal execution were examined using a fixation gap manipulation. We show that following the presentation of a task-irrelevant fearful face, the temporal but not the spatial component of the saccade generation system was affected.
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Emoções/fisiologia , Medo/fisiologia , Desempenho Psicomotor/fisiologia , Movimentos Sacádicos/fisiologia , Colículos Superiores/fisiologia , Adolescente , Tonsila do Cerebelo/fisiologia , Feminino , Fixação Ocular/fisiologia , Humanos , Masculino , Vias Neurais/fisiologia , Testes Neuropsicológicos/normas , Estimulação Luminosa/métodos , Pulvinar/fisiologia , Tempo de Reação/fisiologia , Análise e Desempenho de Tarefas , Fatores de Tempo , Adulto JovemRESUMO
Prior expectations influence how we perceive and recognize objects. However, how they do so remains unclear, especially in the case of real-world complex objects. Expectations of objects may affect which features are used to recognize them subsequently. In this study, we used reverse correlation to reveal with high precision how the use of information across time is modulated by real-world object expectations in a visual recognition task. We show that coarse information leads to accurate responses earlier when an object is expected, indicating that observers use diagnostic features earlier in this situation. We also demonstrate an increased variability in the use of coarse information depending on the expected object, indicating that observers adopt a more specialized recognition strategy when they expect a specific object. In summary, our results reveal potential mechanisms underlying the effect of expectations on the recognition of complex objects.
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Motivação , Reconhecimento Visual de Modelos , Humanos , Reconhecimento PsicológicoRESUMO
The carcass of a critically endangered, juvenile female grey nurse shark (Carcharias taurus, Rafinesque 1810) was recovered from a south-eastern Australian beach and subjected to necropsy. The 1.98-m-long shark exhibited advanced cachexia with its total weight (19.0 kg) and liver weight (0.37 kg) reduced by 60% and 89%, respectively, compared with a healthy individual of the same length. Marked tissue decomposition was evident preventing histopathology and identification of a definitive cause of death. At necropsy, the abdominal organs were abnormally displaced and showed marked reductions in size compared with a healthy individual of the same size. Importantly, a hook-shaped enterolith (HSE), with a rough surface and cream in colour, was found within the spiral valve of the intestine and is to the authors' knowledge, the first description of such in any marine animal. X-ray diffractometry showed that the HSE comprised the minerals monohydrocalcite (Ca[CO3].H2O; ~70 wt%) and struvite (Mg [NH4 ] [PO4 ]. [H2 O]6 ; ~30 wt%). A CT scan showed concentric lamellate concretions around a 7/o offset J-hook that formed the nidus of the HSE. Nylon fishing line attached to the hook exited the HSE and was evident in the abdominal cavity through a perforation in the intestinal wall where the posterior intestinal artery merges. The most parsimonious reconstruction of events leading to enterolithiasis and secondary cachexia in this shark was the consumption of a hooked fish and subsequent hook migration causing perforations of the cardiac stomach wall followed by the thin, muscular wall of the apposed, sub-adjacent intestine.