Characterization of the genome, proteome, and structure of yersiniophage ϕR1-37.

The bacteriophage vB_YecM-ϕR1-37 (ϕR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage ϕR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of ϕR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes scattered throughout the genome. A total of 269 of the ORFs (73%) lack homologues in sequence databases. Based on terminator and promoter sequences identified from the intergenic regions, the phage genome was predicted to consist of 40 to 60 transcriptional units. Image reconstruction revealed that the ϕR1-37 capsid consists of hexameric capsomers arranged on a T=27 lattice similar to the bacteriophage ϕKZ. The tail of ϕR1-37 has a contractile sheath. We conclude that phage ϕR1-37 is a representative of a novel phage type that carries the dU-containing genome in a ϕKZ-like head.

Where one size does not fit all: understanding the needs of potential users of a portal to breast cancer knowledge online.

The article argues that, although the Internet has great potential for assisting people to find information on breast cancer, at present that potential is not being realised. The literature shows considerable dissatisfaction with information provision for breast cancer, including on the Internet where appropriate information suited to particular needs often cannot be found. An Australian project (Breast Cancer Knowledge Online [BCKOnline]), in its first stage, set out to explore the needs for breast cancer information using an ethnographic method and a purposive sample of 77 participants, most of them women with breast cancer. A portal, which will enable users to tailor information to their particular needs, is at present being developed based on the results of the needs analysis. The process includes user-selected profiles, enabled through "user-centric" resource descriptions, and a metadata repository that links the profiles with specific information resources. The article presents limited results from the needs analysis-those highlighting the differences between younger and older women and the problems with present Internet information provision as seen by the sample. The final section discusses how the portal will both tailor information to needs and assist with the problems with the Internet revealed in the literature.