Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros

Base de dados
País/Região como assunto
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
J Virol ; 83(6): 2765-9, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19116254

RESUMO

Herpes simplex virus (HSV) inhibits apoptosis induced by external stimuli in epithelial cells. In contrast, apoptosis is the primary outcome in HSV-infected lymphocytes. Here, we show that HSV type 2 (HSV-2) gene expression appears to be necessary for the induction of apoptosis in Jurkat cells, a T-cell leukemia line. HSV-2 ICP10 gene expression is sufficient to induce apoptosis in Jurkat cells, while its expression protects epithelial HEp-2 cells from apoptosis triggered by cycloheximide and tumor necrosis factor alpha. Thus, the effect of HSV-2 gene expression on the cellular apoptotic pathway appears to depend on the specific cell type.


Assuntos
Apoptose , Herpesvirus Humano 2/fisiologia , Proteínas Serina-Treonina Quinases/fisiologia , Ribonucleotídeo Redutases/fisiologia , Linhagem Celular , Células Epiteliais/virologia , Humanos , Linfócitos T/virologia
2.
Appl Environ Microbiol ; 76(13): 4469-75, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20472727

RESUMO

Coxiella burnetii is an obligate intracellular bacterium that causes the zoonotic disease Q fever. Because C. burnetii is highly infectious, can survive under a variety of environmental conditions, and has been weaponized in the past, it is classified as a select agent and is considered a potential bioweapon. The agent is known to be present in domestic livestock and in wild animal populations, but the background levels of C. burnetii in the environment have not been reported. To better understand the amount of C. burnetii present in the environment of the United States, more than 1,600 environmental samples were collected from six geographically diverse parts of the United States in the years 2006 to 2008. DNA was purified from these samples, and the presence of C. burnetii DNA was evaluated by quantitative PCR of the IS1111 repetitive element. Overall, 23.8% of the samples were positive for C. burnetii DNA. The prevalence in the different states ranged from 6 to 44%. C. burnetii DNA was detected in locations with livestock and also in locations with primarily human activity (post offices, stores, schools, etc.). This study demonstrates that C. burnetii is fairly common in the environment in the United States, and any analysis of C. burnetii after a suspected intentional release should be interpreted in light of these background levels. It also suggests that human exposure to C. burnetii may be more common than what is suggested by the number of reported cases of Q fever.


Assuntos
Coxiella burnetii/genética , Coxiella burnetii/isolamento & purificação , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , Microbiologia Ambiental , Reação em Cadeia da Polimerase/métodos , Animais , Elementos de DNA Transponíveis/genética , Humanos , Camundongos , Prevalência , Estados Unidos/epidemiologia
3.
J Med Entomol ; 39(1): 226-33, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11931262

RESUMO

Female Culex tarsalis Coquillett in reproductive diapause were infected per os or by intrathoracic inoculation with western equine encephalomyelitis (WEE) or St. Louis encephalitis (SLE) viruses during "fall," maintained over a simulated "winter," and then tested for virus infection and transmission in vitro and in vivo after "vernal" termination. Exposure of F1 progeny of field-collected females to cool temperatures and short daylength produced females in reproductive diapause that were reluctant to imbibe infectious virus from pledgets soaked with suspensions of virus, blood and sucrose (2.5% by volume). Those infected per os maintained virus at very low or undetectable titers. Some females that originally tested negative for WEE by plaque assay on Vero cell culture tested positive by reverse transcriptase-polymerase chain reaction (RT-PCR) and by Vero cell culture after passage in mosquito cells. Few females became infected orally with SLE, but these infected females developed elevated titers. Females inoculated with SLE retained their infection through winter and then transmitted readily in vitro and in vivo. Feeding on a vertebrate host after diapause termination significantly increased the titer of SLE in previously infected females. These experiments simulated how infections acquired either horizontally or vertically may provide mechanisms for WEE and SLE overwintering. Attempts to detect infected females during winter following a summer with enzootic WEE activity were negative by both RT-PCR and plaque assay.


Assuntos
Culex/virologia , Vírus da Encefalite de St. Louis/fisiologia , Vírus da Encefalite Equina do Oeste/fisiologia , Insetos Vetores/virologia , Animais , Culex/fisiologia , Vírus da Encefalite de St. Louis/genética , Vírus da Encefalite Equina do Oeste/genética , Feminino , Insetos Vetores/fisiologia , Estações do Ano
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA