RESUMO
Pseudomonas aeruginosa (PA) biofilm-associated infections are a common cause of morbidity in chronic respiratory disease and represent a therapeutic challenge. Recently, the ability of a novel alginate oligomer (OligoG) to potentiate the effect of antibiotics against gram-negative, multi-drug-resistant bacteria and inhibit biofilm formation in vitro has been described. Interaction of OligoG with the cell surface of PA was characterized at the nanoscale using atomic force microscopy (AFM), zeta potential measurement (surface charge), and sizing measurements (dynamic light scattering). The ability of OligoG to modify motility was studied in motility assays. AFM demonstrated binding of OligoG to the bacterial cell surface, which was irreversible after exposure to hydrodynamic shear (5,500 × g). Zeta potential analysis (pH 5-9; 0.1-0.001 M NaCl) demonstrated that binding was associated with marked changes in the bacterial surface charge (-30.9 ± 0.8 to -47.0 ± 2.3 mV; 0.01 M NaCl [pH 5]; P < 0.001). Sizing analysis demonstrated that alteration of surface charge was associated with cell aggregation with a 2- to 3-fold increase in mean particle size at OligoG concentrations greater than 2% (914 ± 284 to 2599 ± 472 nm; 0.01 M NaCl [pH 5]; P < 0.001). These changes were associated with marked dose-dependent inhibition in bacterial swarming motility in PA and Burkholderia spp. The ability of OligoG to bind to a bacterial surface, modulate surface charge, induce microbial aggregation, and inhibit motility represents important direct mechanisms by which antibiotic potentiation and biofilm disruption is affected. These results highlight the value of combining multiple nanoscale technologies to further our understanding of the mechanisms of action of novel antibacterial therapies.
Assuntos
Alginatos/farmacologia , Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Nanomedicina , Pseudomonas aeruginosa/efeitos dos fármacos , Alginatos/química , Antibacterianos/química , Burkholderia/efeitos dos fármacos , Burkholderia/crescimento & desenvolvimento , Química Farmacêutica , Relação Dose-Resposta a Droga , Ácido Glucurônico/química , Ácido Glucurônico/farmacologia , Ácidos Hexurônicos/química , Ácidos Hexurônicos/farmacologia , Luz , Microscopia de Força Atômica , Nanomedicina/métodos , Nanopartículas , Pseudomonas aeruginosa/fisiologia , Espalhamento de Radiação , Propriedades de SuperfícieRESUMO
The influence of a novel, safe antibiofilm therapy on the mechanical properties of Pseudomonas aeruginosa and Acinetobacter baumannii biofilms in vitro was characterized. A multiscale approach employing atomic force microscopy (AFM) and rheometry was used to quantify the mechanical disruption of the biofilms by a therapeutic polymer based on a low-molecular weight alginate oligosaccharide (OligoG). AFM demonstrated structural alterations in the biofilms exposed to OligoG, with significantly lower Young's moduli than the untreated biofilms, (149 MPa vs 242 MPa; p < 0.05), a decreased resistance to hydrodynamic shear and an increased surface irregularity (Ra) in the untreated controls (35.2 nm ± 7.6 vs 12.1 nm ± 5.4; p < 0.05). Rheology demonstrated that increasing clinically relevant concentrations of OligoG (<10%) were associated with an increasing phase angle (δ) over a wide range of frequencies (0.1-10 Hz). These results highlight the utility of these techniques for the study of three-dimensional biofilms and for quantifying novel disruption therapies in vitro.
Assuntos
Acinetobacter baumannii/efeitos dos fármacos , Alginatos/farmacologia , Biofilmes/efeitos dos fármacos , Oligossacarídeos/farmacologia , Pseudomonas aeruginosa/efeitos dos fármacos , Acinetobacter baumannii/fisiologia , Alginatos/química , Alginatos/isolamento & purificação , Aderência Bacteriana/efeitos dos fármacos , Fenômenos Biomecânicos , Módulo de Elasticidade , Hidrodinâmica , Laminaria/química , Testes de Sensibilidade Microbiana , Oligossacarídeos/química , Pseudomonas aeruginosa/fisiologia , Reologia/métodos , Resistência ao Cisalhamento/efeitos dos fármacos , Estresse MecânicoRESUMO
Mechanobiological study of chondrogenic cells and multipotent stem cells for articular cartilage tissue engineering (CTE) has been widely explored. The mechanical stimulation in terms of wall shear stress, hydrostatic pressure and mechanical strain has been applied in CTE in vitro. It has been found that the mechanical stimulation at a certain range can accelerate the chondrogenesis and articular cartilage tissue regeneration. This review explicitly focuses on the study of the influence of the mechanical environment on proliferation and extracellular matrix production of chondrocytes in vitro for CTE. The multidisciplinary approaches used in previous studies and the need for in silico methods to be used in parallel with in vitro methods are also discussed. The information from this review is expected to direct facial CTE research, in which mechanobiology has not been widely explored yet.
RESUMO
Due to the unique physicochemical properties of nanomaterials (NM) and their unknown reactivity, the possibility of NM altering the optical properties of fluorometric/colorimetric probes that are used to measure their cyto- and genotoxicity may lead to inaccurate readings. This could have potential implications given that NM, such as ultrafine superparamagnetic iron oxide nanoparticles (USPION), are increasingly finding their use in nanomedicine and the absorbance/fluorescence based assays are used to assess their toxicity. This study looks at the potential of dextran-coated USPION (dUSPION) (maghemite and magnetite) to alter the background signal of common probes used for evaluating cytotoxicity (MTS, CyQUANT, Calcein, and EthD-1) and oxidative stress (DCFH-DA and APF). In the present study, both forms of dUSPION caused an increase in MTS signal but a decrease in background signal from calcein and 3'-(p-aminophenyl) fluorescein (APF) and no effect on CyQUANT and EthD-1 fluorescence responses. Magnetite caused a decrease in fluorescence signal of DCFH, but it did not decrease fluorescence signal in the presence of the reactive oxygen species-inducer tert-butyl hydroperoxide (TBHP). In contrast, maghemite caused an increase in fluorescence, which was substantially reduced in the presence of the antioxidant N-acetyl cysteine. This study emphasizes the importance of considering and controlling for possible interactions between NM and fluorometric/colorimetric dyes and, most importantly, the oxidation state of dUSPION that may confound their sensitivity and specificity.
Assuntos
Colorimetria/métodos , Corantes/química , Dextranos/química , Compostos Férricos/química , Corantes Fluorescentes/química , Fluorometria/métodos , Nanopartículas de Magnetita/química , Etídio/análogos & derivados , Etídio/toxicidade , Fluoresceínas/toxicidade , Espécies Reativas de Oxigênio/metabolismo , terc-Butil Hidroperóxido/químicaRESUMO
Marine goniomonads have a worldwide distribution but ultrastructural information has not been available so far. An isolate of the heterotrophic marine nanoflagellate Goniomonas (G. aff. amphinema) from North Wales (UK) has been studied, providing information on its morphology and cellular structure using video, electron, laser scanning confocal microscopy (LSCM), and atomic force microscopy. Here, we describe a new feature, a granular area, potentially involved in particle capture and feeding. The binding of the lectin wheat germ agglutinin to the granular area of cells with discharged ejectisomes indicates the adhesive nature of this novel feature. The presence of a microtubular intracellular cytopharynx, apparently also used for feeding, has been revealed by LSCM. The small subunit rRNA gene of the isolate has been sequenced (1,788 bp). Phylogenetic results corroborate significant genetic divergence within the marine members of Goniomonas. This work highlights the need for integrated morphological, ultrastructural, and molecular investigation when describing and studying heterotrophic nanoflagellates.
Assuntos
Criptófitas/classificação , Criptófitas/citologia , Água do Mar/parasitologia , Análise por Conglomerados , Criptófitas/genética , Criptófitas/isolamento & purificação , DNA de Algas/química , DNA de Algas/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Comportamento Alimentar , Genes de RNAr , Lectinas/metabolismo , Microscopia de Força Atômica , Microscopia Confocal , Microscopia Eletrônica , Microscopia de Vídeo , Filogenia , Ligação Proteica , RNA de Algas/genética , RNA Ribossômico 18S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , País de Gales , Aglutininas do Germe de Trigo/metabolismoRESUMO
AFM (atomic force microscopy) analysis, both of fixed cells, and live cells in physiological environments, is set to offer a step change in the research of cellular function. With the ability to map cell topography and morphology, provide structural details of surface proteins and their expression patterns and to detect pico-Newton force interactions, AFM represents an exciting addition to the arsenal of the cell biologist. With the explosion of new applications, and the advent of combined instrumentation such as AFM-confocal systems, the biological application of AFM has come of age. The use of AFM in the area of biomedical research has been proposed for some time, and is one where a significant impact could be made. Fixed cell analysis provides qualitative and quantitative subcellular and surface data capable of revealing new biomarkers in medical pathologies. Image height and contrast, surface roughness, fractal, volume and force analysis provide a platform for the multiparameter analysis of cell and protein functions. Here, we review the current status of AFM in the field and discuss the important contribution AFM is poised to make in the understanding of biological systems.
Assuntos
Técnicas Citológicas/métodos , Microscopia de Força Atômica/métodos , Animais , Tecnologia Biomédica , Sobrevivência Celular , Humanos , Microscopia de Força Atômica/instrumentação , Microscopia Eletrônica , Fixação de TecidosRESUMO
BACKGROUND INFORMATION: The endometrial epithelial cell membrane is a key interface in female reproductive biology. Steroid hormones play a predominant role in cyclic changes which occur at this interface during the female menstrual cycle. Specific changes in the morphology of the endometrial epithelial cell surface become apparent with the epithelial transition that drives the switch from a non-receptive to receptive surface due to the action of progesterone on an oestrogen primed tissue. AFM (atomic force microscopy) allows the high-resolution characterization of the endometrial epithelial cell surface. Its contact probe mechanism enables a unique imaging method that requires little sample preparation, yielding topographical and morphological characterization. By stiffening the cell membrane, low concentrations of fixatives allow the surface detail of the cell to be resolved while preserving fine ultra-structural details for analysis. RESULTS: In the present study we use high resolution AFM analysis of endometrial epithelial cells to monitor the effect of progesterone on the nanoscale structure of the endometrial cell surface. High-resolution imaging reveals similar topographical nanoscale changes in both the Hec-1-A and Ishikawa model cell lines. Hec-1-B cells, used in the present study as a progesterone receptor negative control, however, exhibit a flattened cell surface morphology following progesterone treatment. Changes in average cell height and surface convolution correlate with increased surface roughness measurements, demonstrating alterations in molecular structure on the cell surface due to hormonal stimulation. CONCLUSIONS: Progesterone treatment induces changes to the cell surface as a result of nanoscale molecular modifications in response to external hormonal treatments. AFM provides the basis for the identification, visualization and quantification of these cell surface nanoscale changes. Together these findings demonstrate the utility of AFM for use in reproductive science and cancer biology where it could be applied in both in vitro analysis of protein structure-function relationships and clinical diagnosis.
Assuntos
Endométrio/química , Endométrio/metabolismo , Células Epiteliais/química , Células Epiteliais/metabolismo , Progesterona/metabolismo , Linhagem Celular Tumoral , Células Cultivadas , Endométrio/citologia , Células Epiteliais/citologia , Feminino , Humanos , Microscopia de Força AtômicaRESUMO
Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges. The scaffolds were prepared and examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and scanning electron microscopy (SEM). The samples were implanted in adult male Wistar rats for in vivo experimentation. Both crosslinked and uncrosslinked jellyfish collagen sponges showed a significant reduction in histopathology scores over the course of the study, whereas the bovine collagen sponge scores were not significantly reduced. Both jellyfish collagen sponges and the bovine sponge were tolerated well by the hosts, and a recovery was visible in all samples, suggesting that R. pulmo jellyfish-derived collagen could offer compelling biocompatibility with wound healing applications. We also demonstrate that noncrosslinked samples could be safer with better resorption times than crosslinked samples. © 2017 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1524-1533, 2018.
Assuntos
Bandagens , Materiais Biocompatíveis , Colágeno , Teste de Materiais , Cifozoários/química , Cicatrização/efeitos dos fármacos , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Bovinos , Colágeno/química , Colágeno/farmacologia , Masculino , Ratos , Ratos WistarRESUMO
Repeated subculturing caused rapid changes in the spore surface properties and virulence of Metarhizium anisopliae. Of the two strains evaluated, M. anisopliae V245 attenuated more rapidly than V275. Electrophoretic mobility and Radial Flow Chamber assays were used for the first time to generate qualitative and quantitative information on the adhesive forces of M. anisopliae conidia. Independent of strain, adhesion, hydrophobicity and spore-bound Pr1 declined after the first subculture; however, spore surface charge decline was erratic. Adhesion and hydrophobicity stabilized after the third subculture, whereas spore-bound Pr1 continues to decline following repeated subculturing. Decline in spore bound Pr1 was directly correlated with decline in virulence, however, such correlation with adhesion, hydrophobicity or surface charge could not be established. Because spore-bound Pr1 activities were directly correlated with M. anisopliae virulence; it could be used as a quality-control marker to monitor changes in virulence.
Assuntos
Metarhizium/química , Metarhizium/patogenicidade , Esporos Fúngicos/química , Animais , Adesão Celular/fisiologia , Proteínas Fúngicas/análise , Interações Hidrofóbicas e Hidrofílicas , Larva/microbiologia , Dados de Sequência Molecular , Serina Endopeptidases/análise , Propriedades de Superfície , Análise de Sobrevida , Tenebrio/microbiologia , VirulênciaRESUMO
This paper reviews the use of iron oxide nanoparticle-nanofiber composites in tissue engineering with a focus on the electrospinning technique. Electrospinning is an established method of scaffold fabrication offering a number of key advantages which include its facile nature, with electrospun materials offering a high surface area to volume ratio, potential for the release of drugs and antimicrobials, controllable fiber diameters and high porosity and permeability. A number of different techniques for the preparation of iron oxide nanoparticles including their functionalization are discussed along with their applications in the biomedical field. The review then focusses on the fabrication of nanoparticle-nanofiber composite scaffolds formed using electrospinning. The advantages and disadvantages of current fabrication techniques are discussed including the fabrication of nanofibers using pre-synthesized nanoparticles and post-treatment synthesized nanoparticles. We demonstrate that emerging in-situ synthesis techniques show promise by offering a reduced number of steps and simpler procedures for the production of magnetic scaffolds. These scaffolds have a number of applications in tissue engineering, allowing for improved bone and tissue repair.
Assuntos
Técnicas Eletroquímicas/instrumentação , Compostos Férricos/química , Nanofibras/química , Nanopartículas/química , Materiais Biocompatíveis , Tamanho da Partícula , Engenharia Tecidual , Alicerces TeciduaisRESUMO
The detrimental effect of bacterial biofilms on process engineering surfaces is well documented. Thus, interest in the early stages of bacterial biofilm formation; in particular bacterial adhesion and the production of anti-fouling coatings has grown exponentially as a field. During this time, Atomic force microscopy (AFM) has emerged as a critical tool for the evaluation of bacterial adhesion. Due to its versatility AFM offers not only insight into the topographical landscape and mechanical properties of the engineering surfaces, but elucidates, through direct quantification the topographical and biomechnical properties of the foulants The aim of this review is to collate the current research on bacterial adhesion, both theoretical and practical, and outline how AFM as a technique is uniquely equipped to provide further insight into the nanoscale world at the bioprocess engineering surface.
Assuntos
Fenômenos Fisiológicos Bacterianos , Biofilmes/crescimento & desenvolvimento , Microscopia de Força Atômica/métodos , Aderência Bacteriana , Carga Bacteriana , Fenômenos Biomecânicos , Propriedades de SuperfícieRESUMO
We demonstrate a facile, one-step process to form polymer scaffolds composed of magnetic iron oxide nanoparticles (MNPs) contained within electrospun nano- and micro-fibres of two biocompatible polymers, Poly(ethylene oxide) (PEO) and Poly(vinyl pyrrolidone) (PVP). This was achieved with both needle and free-surface electrospinning systems demonstrating the scalability of the composite fibre manufacture; a 228 fold increase in fibre fabrication was observed for the free-surface system. In all cases the nanoparticle-nanofibre composite scaffolds displayed morphological properties as good as or better than those previously described and fabricated using complex multi-stage techniques. Fibres produced had an average diameter (Needle-spun: 125±18nm (PEO) and 1.58±0.28µm (PVP); Free-surface electrospun: 155±31nm (PEO)) similar to that reported previously, were smooth with no bead defects. Nanoparticle-nanofibre composites were characterised using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS) (Nanoparticle average diameter ranging from 8±3nm to 27±5nm), XRD (Phase of iron oxide nanoparticles identified as magnetite) and nuclear magnetic resonance relaxation measurements (NMR) (T1/T2: 32.44 for PEO fibres containing MNPs) were used to verify the magnetic behaviour of MNPs. This study represents a significant step forward for production rates of magnetic nanoparticle-nanofibre composite scaffolds by the electrospinning technique.
Assuntos
Compostos Férricos/química , Nanofibras/química , Nanopartículas/química , Engenharia Tecidual/métodos , Difusão Dinâmica da Luz , Espectroscopia de Ressonância Magnética , Nanofibras/ultraestrutura , Nanopartículas/ultraestrutura , Tamanho da Partícula , Polietilenoglicóis/química , Povidona/química , Espectrometria por Raios X , Espectrofotometria Atômica , Difração de Raios XRESUMO
Concerns about acquisition of antibiotic resistance have led to increasing demand for new antimicrobial therapies. OligoG CF-5/20 is an alginate oligosaccharide previously shown to have antimicrobial and antibiotic potentiating activity. We investigated the structural modification of the bacterial cell wall by OligoG CF-5/20 and its effect on membrane permeability. Binding of OligoG CF-5/20 to the bacterial cell surface was demonstrated in Gram-negative bacteria. Permeability assays revealed that OligoG CF-5/20 had virtually no membrane-perturbing effects. Lipopolysaccharide (LPS) surface charge and aggregation were unaltered in the presence of OligoG CF-5/20. Small angle neutron scattering and circular dichroism spectroscopy showed no substantial change to the structure of LPS in the presence of OligoG CF-5/20, however, isothermal titration calorimetry demonstrated a weak calcium-mediated interaction. Metabolomic analysis confirmed no change in cellular metabolic response to a range of osmolytes when treated with OligoG CF-5/20. This data shows that, although weak interactions occur between LPS and OligoG CF-5/20 in the presence of calcium, the antimicrobial effects of OligoG CF-5/20 are not related to the induction of structural alterations in the LPS or cell permeability. These results suggest a novel mechanism of action that may avoid the common route in acquisition of resistance via LPS structural modification.
Assuntos
Alginatos/farmacologia , Anti-Infecciosos/farmacologia , Membrana Celular/metabolismo , Pseudomonas aeruginosa/citologia , Streptococcus mutans/citologia , Alginatos/química , Cátions Bivalentes/farmacologia , Membrana Celular/efeitos dos fármacos , Permeabilidade da Membrana Celular/efeitos dos fármacos , Parede Celular/efeitos dos fármacos , Parede Celular/metabolismo , Ácido Glucurônico/química , Ácido Glucurônico/farmacologia , Ácidos Hexurônicos/química , Ácidos Hexurônicos/farmacologia , Lipopolissacarídeos/química , Lipopolissacarídeos/farmacologia , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Streptococcus mutans/efeitos dos fármacosRESUMO
Nanocellulose from wood is a novel biomaterial, which is highly fibrillated at the nanoscale. This affords the material a number of advantages, including self-assembly, biodegradability and the ability to absorb and retain moisture, which highlights its potential usefulness in clinical wound-dressing applications. In these in vitro studies, the wound pathogen Pseudomonas aeruginosa PAO1 was used to assess the ability of two nanocellulose materials to impair bacterial growth (<48 h). The two nanocelluloses had a relatively small fraction of residual fibres (<4%) and thus a large fraction of nanofibrils (widths <20 nm). Scanning electron microscopy and confocal laser scanning microscopy imaging demonstrated impaired biofilm growth on the nanocellulose films and increased cell death when compared to a commercial control wound dressing, Aquacel(®). Nanocellulose suspensions inhibited bacterial growth, whilst UV-vis spectrophotometry and laser profilometry also revealed the ability of nanocellulose to form smooth, translucent films. Atomic force microscopy studies of the surface properties of nanocellulose demonstrated that PAO1 exhibited markedly contrasting morphology when grown on the nanocellulose film surfaces compared to an Aquacel(®) control dressing (p<0.05). This study highlights the potential utility of these biodegradable materials, from a renewable source, for wound dressing applications in the prevention and treatment of biofilm development.
Assuntos
Biofilmes/crescimento & desenvolvimento , Celulose/química , Nanoestruturas/química , Pseudomonas aeruginosa/crescimento & desenvolvimento , Microscopia de Força AtômicaRESUMO
The present study demonstrates the high potential for the application of a novel self assembled positively charged nanofiltration membrane, PA6DT-C, in processes such as the recovery of valuable cationic macromolecules in the bioprocess and pharmaceutical industries or removal of multi-valent cations such as dyes and heavy metals in the paper and pulp, textiles, nuclear, and automotive industries. The nanofiltration membrane, prepared in this laboratory, is further characterised and then tested for the removal and recovery of Methylene Blue from a synthetic dye house wastewater. The characterisation process involved the construction of a rejection profile for NaCl over a wide range of pH and concentration, which illustrates that the optimal process conditions for the removal of small cations using this membrane is in the region pH <8.0 and concentration less than 15 mol m(-3). The salt rejection data was used to calculate the magnitude of the effective membrane charge density and this was found to be significantly higher for the PA6DT-C membrane than two commercially available membranes (Desal-DK and Nanomax-50). The membrane flux for this new membrane is also superior to the commercial membranes with an approximate increase of 3-4 fold. The PA6DT-C membrane was successful in removal of Methylene Blue dye from synthetic dye house wastewaters achieving 98% rejection and a membrane flux of ≈ 17 LMH bar(-1). Thus, this new membrane both adds to and complements the existing short supply of positively charged NF membranes.
Assuntos
Filtração/métodos , Resíduos Industriais/análise , Membranas Artificiais , Nanotecnologia/métodos , Indústria Têxtil , Eliminação de Resíduos Líquidos , Purificação da Água/métodos , Cor , Eletricidade , Estudos de Viabilidade , Concentração de Íons de Hidrogênio , Azul de Metileno/isolamento & purificação , Porosidade , Reciclagem , Cloreto de Sódio/químicaRESUMO
Ultrafine superparamagnetic iron oxide nanoparticles (USPION) hold great potential for revolutionising biomedical applications such as MRI, localised hyperthermia, and targeted drug delivery. Though evidence is increasing regarding the influence of nanoparticle physico-chemical features on toxicity, data however, is lacking that assesses a range of such characteristics in parallel. We show that iron redox state, a subtle though important physico-chemical feature of USPION, dramatically modifies the cellular uptake of these nanoparticles and influences their induction of DNA damage. Surface chemistry was also found to have an impact and evidence to support a potential mechanism of oxidative DNA damage behind the observed responses has been demonstrated. As human exposure to ferrofluids is predicted to increase through nanomedicine based therapeutics, these findings are important in guiding the fabrication of USPION to ensure they have characteristics that support biocompatibility.
Assuntos
Compostos Férricos/química , Nanopartículas de Magnetita/química , Linhagem Celular , Dano ao DNA/efeitos dos fármacos , Compostos Férricos/efeitos adversos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Nanopartículas de Magnetita/efeitos adversos , Nanopartículas de Magnetita/ultraestrutura , Microscopia Eletrônica de Transmissão , Oxirredução , Espectroscopia FotoeletrônicaRESUMO
A review of the fabrication processes currently available to produce positively charged nanofiltration membranes has been conducted. The review highlights that there are few membranes and studies currently available. The preparation of a novel positively charged nanofiltration membrane is also described. This membrane was fabricated by surface modification of a prepared base membrane using polyethyleneimine followed by cross linking with butanedioldiglycidylether. The fabrication process uses standard organic solvents and avoids the need for hazardous materials, such as concentrated sulphuric acid, which significantly benefits the scale up potential of any future commercial manufacturing process. The new membrane was characterised using a number of state-of-the-art techniques, including a novel use of atomic force microscopy to determine pore size. Streaming potential measurements confirmed that this new membrane is indeed positively charged in the pH range below pH 9, which covers the majority of normal operating conditions. The performance characteristics for the new membrane were very favourable, with a pure water flux determined to be 20 LMH bar(-1) and a rejection of MgCl of 96%. Thus, this new membrane both adds to and complements the existing short supply of positively charged NF membranes and is suitable for applications such as the recovery of valuable cationic macromolecules in the bioprocess and pharmaceutical industries or removal of multi-valent cations such as dyes and heavy metals in the paper and pulp, textiles, nuclear, and automotive industries.
RESUMO
Atomic Force Microscopy (AFM) has proven itself over recent years as an essential tool for the analysis of microbial systems. This article will review how AFM has been used to study microbial systems to provide unique insight into their behavior and relationship with their environment. Immobilization of live cells has enabled AFM imaging and force measurement to provide understanding of the structure and function of numerous microbial cells. At the macromolecular level AFM investigation into the properties of surface macromolecules and the energies associated with their mechanical conformation and functionality has helped unravel the complex interactions of microbial cells. At the level of the whole cell AFM has provided an integrated analysis of how the microbial cell exploits its environment through its selective, adaptable interface, the cell surface. In addition to these areas of study the AFM investigation of microbial biofilms has been vital for industrial and medical process analysis. There exists a tremendous potential for the future application of AFM to microbial systems and this has been strengthened by the trend to use AFM in combination with other characterization methods, such as confocal microscopy and Raman spectroscopy, to elucidate dynamic cellular processes.
Assuntos
Bactérias/ultraestrutura , Fenômenos Fisiológicos Bacterianos , Biofilmes , Microscopia de Força Atômica/métodosRESUMO
With the rapid expansion in the nanotechnology industry, it is essential that the safety of engineered nanomaterials and the factors that influence their associated hazards are understood. A vital area governing regulatory health risk assessment is genotoxicology (the study of genetic aberrations following exposure to test agents), as DNA damage may initiate and promote carcinogenesis, or impact fertility. Of late, considerable attention has been given to the toxicity of engineered nanomaterials, but the importance of their genotoxic potential on human health has been largely overlooked. This comprehensive review focuses on the reported abilities of metal nanoparticles, metal-oxide nanoparticles, quantum dots, fullerenes, and fibrous nanomaterials, to damage or interact with DNA, and their ecogenotoxicity is also considered. Many of the engineered nanomaterials assessed were found to cause genotoxic responses, such as chromosomal fragmentation, DNA strand breakages, point mutations, oxidative DNA adducts and alterations in gene expression profiles. However, there are clear inconsistencies in the literature and it is difficult to draw conclusions on the physico-chemical features of nanomaterials that promote genotoxicity, largely due to study design. Hence, areas that require that further attention are highlighted and recommendations to improve our understanding of the genotoxic potential of engineered nanomaterials are addressed.
Assuntos
Dano ao DNA/efeitos dos fármacos , Nanoestruturas/toxicidade , Animais , Humanos , Nanopartículas Metálicas/toxicidade , Nanotecnologia , Nanotubos/toxicidade , Pontos QuânticosRESUMO
An atomic force microscope (AFM) has been used to quantify directly the adhesion of metabolically active Saccharomyces cerevisiae cells at a hydrophilic mica surface, a mica surface with a hydrophobic coating, and a protein-coated mica surface in an aqueous environment. The measurements used "cell probes" constructed by immobilizing a single cell at the apex of a tipless AFM cantilever. Adhesion was quantified from force-distance data for the retraction of the cell from the surface. The data indicated stretching and sequential bond-breaking as the cell probe was retracted from all of the surfaces. Detailed studies were made for physiologically active cells, which were shown to have different adhesion properties to glutaraldehyde-treated cells. Greatest cell adhesion was measured at the hydrophobic surface. Prior adsorption of a bovine serum albumin protein layer at the hydrophilic surface did not significantly affect cell adhesion. Changes in yeast surface hydrophobicity and zeta-potential with yeast cell age were correlated with differences in adhesion. Cells from the stationary phase adhered most strongly to a mica surface. Time of surface contact was demonstrated to be important. Both the force needed to detach a cell from a hydrophilic mica surface and the length of the adhesive interaction increased after 5 min contact. The AFM cell probe technique gives unique insights into primary colonization events in biofilm formation. It will continue to aid both fundamental studies and the assessment of new procedures that are designed to lower cell adhesion at surfaces relevant to biotechnology, medicine, and dentistry Copyright 2001 Academic Press.