RESUMO
Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F.tataricum exhibited good functions for free radicals scavengingï¼ anti-oxidationï¼ anti-aging activities. Although much genetic knowledge of the synthesisï¼ regulationï¼ accumulation of rutinï¼ the genetic basis of proanthocyanidins(PAs) in tartary buckwheat and their related gene expression changes under different lights(blueï¼ redï¼ far redï¼ ultraviolet light) remain largely unexplored. In this studyï¼ we cloned one anthocyanidin reductase gene(ANR) and two leucocyanidin reductase gene(LAR) named FtANRï¼FtLAR1ï¼FtLAR3 involved in formation of(+)-catechin and(-)-epicatechin precusor proanthocyanidin by digging out F. tataricum seed transcriptome data. The expression data showed that the opposite influence of red light on these gene transcript level compared to others lights. The expression levels of FtANR and FtLAR1 decreased and FtLAR3 appeared increment after exposed in the red lightï¼ while the expression levels of those genes appeared opposite result after exposed in the blue and far red light.
Assuntos
Fagopyrum/enzimologia , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Luz , Proantocianidinas/biossíntese , Fagopyrum/efeitos da radiação , NADH NADPH Oxirredutases/genética , Proteínas de Plantas/genética , Sementes/enzimologia , Sementes/efeitos da radiaçãoRESUMO
Chimeric antigen receptor (CAR)-T cells have shown great promise in cancer therapy. However, the anti-tumor efficiency is limited due to the CAR-induced T cell apoptosis or exhaustion. The intracellular domain of CAR comprised of various signaling modules orchestrates CAR-T cell behaviors. The modularity of CAR signaling domain functions as the "mainboard" to assemble diversified downstream signaling components. Here, we implemented the modular recombination strategy to construct a library of CARs with synthetic co-signaling modules adopted from immunoglobin-like superfamily (IgSF) and tumor necrosis factor receptor superfamily (TNFRSF). We quantitatively characterized the signaling behaviors of these recombinants by both NFAT and NF-κB reporter, and identified a set of new CARs with diverse signaling behaviors. Specifically, the 28(NM)-BB(MC) CAR-T cells exhibited improved cytotoxicity and T cell persistence. The synthetic approach can promote our understanding of the signaling principles of CAR molecule, and provide a powerful tool box for CAR-T cell engineering.