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J Gen Virol ; 96(11): 3444-3455, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26358706

RESUMO

The presence of disease-associated prions in tissues and bodily fluids of chronic wasting disease (CWD)-infected cervids has received much investigation, yet little is known about mother-to-offspring transmission of CWD. Our previous work demonstrated that mother-to-offspring transmission is efficient in an experimental setting. To address the question of relevance in a naturally exposed free-ranging population, we assessed maternal and fetal tissues derived from 19 elk dam-calf pairs collected from free-ranging Rocky Mountain elk from north-central Colorado, a known CWD endemic region. Conventional immunohistochemistry identified three of 19 CWD-positive dams, whereas a more sensitive assay [serial protein misfolding cyclic amplification (sPMCA)] detected CWD prion seeding activity (PrPCWD) in 15 of 19 dams. PrPCWD distribution in tissues was widespread, and included the central nervous system (CNS), lymphoreticular system, and reproductive, secretory, excretory and adipose tissues. Interestingly, five of 15 sPMCA-positive dams showed no evidence of PrPCWD in either CNS or lymphoreticular system, sites typically assessed in diagnosing CWD. Analysis of fetal tissues harvested from the 15 sPMCA-positive dams revealed PrPCWD in 80 % of fetuses (12 of 15), regardless of gestational stage. These findings demonstrated that PrPCWD is more abundant in peripheral tissues of CWD-exposed elk than current diagnostic methods suggest, and that transmission of prions from mother to offspring may contribute to the efficient transmission of CWD in naturally exposed cervid populations.


Assuntos
Animais Selvagens/metabolismo , Cervos/metabolismo , Transmissão Vertical de Doenças Infecciosas , Doenças Priônicas/transmissão , Príons/metabolismo , Doença de Emaciação Crônica/transmissão , Animais , Sistema Nervoso Central/metabolismo , Colorado , Cervos/embriologia , Feminino , Masculino , Doenças Priônicas/embriologia , Doenças Priônicas/metabolismo , Príons/genética , Distribuição Tecidual , Doença de Emaciação Crônica/embriologia , Doença de Emaciação Crônica/metabolismo
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