Submegahertz Nucleation of Plasmonic Vapor Microbubbles near a Solid Vertical Boundary.

Laser triggered and photothermally induced vapor bubbles have emerged as promising approaches to facilitate optomechanical energy conversion for numerous applications in microfluidics and nanofluidics. Here, we report an observation of spontaneously triggered periodic nucleation of plasmonic vapor bubbles near a rigid sidewall with readily tuned nucleation frequency from 0.8 kHz to over 200 kHz. The detailed collapsing process of the vapor bubbles was experimentally and numerically investigated. We find that the lateral migration of residual bubbles toward the sidewall refreshes the laser spot area, terminates the subsequent steady bubble growth, and leads to the repeatable bubble nucleation. A mathematic model regarding the Kelvin impulses was derived. It shows that the competition between the rigid boundary induced Bjerknes force and laser irradiation caused thermal Marangoni force on collapsing bubbles governs the process. The model also leads to a criterion of γζ<0.34 for repeatable bubble nucleation, where γ is the normalized distance and ζ thermal Marangoni coefficient. This study demonstrates nucleation of violent vapor bubbles at extreme high frequencies, providing an approach to remotely realize strong localized flows in microfluidics and nanofluidics.

Laser Controlled Manipulation of Microbubbles on a Surface with Silica-Coated Gold Nanoparticle Array.

Microbubble generation and manipulation play critical roles in diverse applications such as microfluidic mixing, pumping, and microrobot propulsion. However, existing methods are typically limited to lateral movements on customized substrates or rely on specific liquids with particular properties or designed concentration gradients, thereby hindering their practical applications. To address this challenge, this paper presents a method that enables robust vertical manipulation of microbubbles. By focusing a resonant laser on hydrophilic silica-coated gold nanoparticle arrays immersed in water, plasmonic microbubbles are generated and detach from the substrates immediately upon cessation of laser irradiation. Using simple laser pulse control, it can achieve an adjustable size and frequency of bubble bouncing, which is governed by the movement of the three-phase contact line during surface wetting. Furthermore, it demonstrates that rising bubbles can be pulled back by laser irradiation induced thermal Marangoni flow, which is verified by particle image velocimetry measurements and numerical simulations. This study provides novel insights into flexible bubble manipulation and integration in microfluidics, with significant implications for various applications including mixing, drug delivery, and the development of soft actuators.

Long Noncoding RNA-1604 Orchestrates Neural Differentiation through the miR-200c/ZEB Axis.

Clarifying the regulatory mechanisms of embryonic stem cell (ESC) neural differentiation is helpful not only for understanding neural development but also for obtaining high-quality neural progenitor cells required by stem cell therapy of neurodegenerative diseases. Here, we found that long noncoding RNA 1604 (lncRNA-1604) was highly expressed in cytoplasm during neural differentiation, and knockdown of lncRNA-1604 significantly repressed neural differentiation of mouse ESCs both in vitro and in vivo. Bioinformatics prediction and mechanistic analysis revealed that lncRNA-1604 functioned as a novel competing endogenous RNA of miR-200c and regulated the core transcription factors ZEB1 and ZEB2 during neural differentiation. Furthermore, we also demonstrated the critical role of miR-200c and ZEB1/2 in mouse neural differentiation. Either introduction of miR-200c sponge or overexpression of ZEB1/2 significantly reversed the lncRNA-1604 knockdown-induced repression of mouse ESC neural differentiation. Collectively, these findings not only identified a previously unknown role of lncRNA-1604 and ZEB1/2 but also elucidated a new regulatory lncRNA-1604/miR-200c/ZEB axis in neural differentiation. Stem Cells 2018;36:325-336.

Temperature Dependence of Internal Friction of Peptides.

Internal friction is a valuable concept to describe the kinetics of proteins. As is well known, internal friction can be modulated by solvent features (such as viscosity). How can internal friction be affected by environmental temperature? The answer to this question is not evident. In the present work, we approach this problem with simulations on two model peptides. The thermodynamics and relaxation kinetics are characterized through long molecular dynamics simulations, with the viscosity modulated by varying the mass of solvent molecules. Based on the extrapolation to zero viscosity together with scaling of the relaxation time scales, we discover that internal friction is almost invariant at various temperatures. Controlled simulations further support the idea that internal friction is independent of environmental temperature. Comparisons between the two model peptides help us to understand the diverse phenomena in experiments.

Origin of subdiffusions in proteins: Insight from peptide systems.

Subdiffusive kinetics are popular in proteins and peptides as observed in experiments and simulations. For protein systems with diverse interactions, are there multiple mechanisms to produce the common subdiffusion behavior? To approach this problem, long trajectories of two model peptides are simulated to study the mechanism of subdiffusion and the relations with their interactions. The free-energy profiles and the subdiffusive kinetics are observed for these two peptides. A hierarchical plateau analysis is employed to extract the features of the landscape from the mean square of displacement. The mechanism of subdiffusions can be postulated by comparing the exponents by simulations with those based on various models. The results indicate that the mechanisms of these two peptides are different and are related to the characteristics of their energy landscapes. The subdiffusion of the flexible peptide is mainly caused by depth distribution of traps on the energy landscape, while the subdiffusion of the helical peptide is attributed to the fractal topology of local minima on the landscape. The emergence of these different mechanisms reflects different kinetic scenarios in peptide systems though the peptides behave in a similar way of diffusion. To confirm these ideas, the transition networks between various conformations of these peptides are generated. Based on the network description, the controlled kinetics based only on the topology of the networks are calculated and compared with the results based on simulations. For the flexible peptide, the feature of controlled diffusion is distinct from that of simulation, and for the helical peptide, two kinds of kinetics have a similar exponent of subdiffusion. These results further exemplify the importance of the landscape topology in the kinetics of structural proteins and the effect of depth distribution of traps for the subdiffusion of disordered peptides.

Giant plasmonic bubbles nucleation under different ambient pressures.

Water-immersed gold nanoparticles irradiated by a laser can trigger the nucleation of plasmonic bubbles after a delay time of a few microseconds [Wang et al., Proc. Natl. Acad. Sci. USA 122, 9253 (2018)]. Here we systematically investigated the light-vapor conversion efficiency, η, of these plasmonic bubbles as a function of the ambient pressure. The efficiency of the formation of these initial-phase and mainly water-vapor containing bubbles, which is defined as the ratio of the energy that is required to form the vapor bubbles and the total energy dumped in the gold nanoparticles before nucleation of the bubble by the laser, can be as high as 25%. The amount of vaporized water first scales linearly with the total laser energy dumped in the gold nanoparticles before nucleation, but for larger energies the amount of vaporized water levels off. The efficiency η decreases with increasing ambient pressure. The experimental observations can be quantitatively understood within a theoretical framework based on the thermal diffusion equation and the thermal dynamics of the phase transition.