Bioelectrochemical acidolysis of magnesia to induce struvite crystallization for recovering phosphorus from aqueous solution.

A novel struvite crystallization method induced by bioelectrochemical acidolysis of magnesia (MgO) was investigated to recover phosphorus (P) from aqueous solution using a dual-chamber microbial electrolysis cell (DMEC). Magnesium ion (Mg2+) in the anolyte was firstly confirmed to automatically migrate from the anode chamber to the cathode chamber, and then react with ammonium (NH4+) and phosphate (PO43-) in the catholyte to form struvite. Recovery efficiency of 17.8%-60.2% was obtained with the various N/P ratios in the catholyte. When MgO (low solubility under alkali conditions) was added into the anolyte, the bioelectrochemical acidolysis of MgO naturally took place and the released Mg2+ induced struvite crystallization in the cathode chamber for P recovery likewise. Besides, there was a strong linear positive correlation between the recovery efficiency and the MgO dosage (R2 = 0.935), applied voltage (R2 = 0.969) and N/P ratio (R2 = 0.905). Increasing the applied voltage was found to enhance the P recovery via promoting the MgO acidolysis and the released Mg2+ migration, while increasing the N/P ratio in the catholyte enhanced the P recovery via promoting the struvite crystallization. Moreover, the electrochemical performance of the system was promoted due to more stable anolyte pH and lower pH gradient between the two chambers. Current density was promoted by 10%, while the COD removal efficiency was improved from 78.2% to 91.8% in the anode chamber.

Reconstitution of the Multiple Myeloma Microenvironment Following Lymphodepletion with BCMA CAR-T Therapy.

PURPOSE: The purpose of this study was to investigate the remodeling of the multiple myeloma microenvironment after B-cell maturation antigen (BCMA)-targeted chimeric antigen receptor T (CAR-T) cell therapy. EXPERIMENTAL DESIGN: We performed single-cell RNA sequencing on paired bone marrow specimens (n = 14) from seven patients with multiple myeloma before (i.e., baseline, "day -4") and after (i.e., "day 28") lymphodepleted BCMA CAR-T cell therapy. RESULTS: Our analysis revealed heterogeneity in gene expression profiles among multiple myeloma cells, even those harboring the same cytogenetic abnormalities. The best overall responses of patients over the 15-month follow-up are positively correlated with the abundance and targeted cytotoxic activity of CD8+ effector CAR-T cells on day 28 after CAR-T cell infusion. Additionally, favorable responses are associated with attenuated immunosuppression mediated by regulatory T cells, enhanced CD8+ effector T-cell cytotoxic activity, and elevated type 1 conventional dendritic cell (DC) antigen presentation ability. DC re-clustering inferred intramedullary-originated type 3 conventional DCs with extramedullary migration. Cell-cell communication network analysis indicated that BCMA CAR-T therapy mitigates BAFF/GALECTIN/MK pathway-mediated immunosuppression and activates MIF pathway-mediated anti-multiple myeloma immunity. CONCLUSIONS: Our study sheds light on multiple myeloma microenvironment dynamics after BCMA CAR-T therapy, offering clues for predicting treatment responsivity.

Vibrational spectra analysis of amorphous lactose in structural transformation: Water/temperature plasticization, crystal formation, and molecular mobility.

Lactose is a common component found in many foods and dairy products. In this study, the vibrational signatures in the crystalline structure of α-, ß-, and α-lactose monohydrate were calculated based on quantum chemistry calculation (QCC), whilst the vibrational spectra in freeze-dried lactose equilibrated at various aw and pre-humidified amorphous lactose (0.33 aw) stored from 25 to 95 °C were determined by using Raman and FT-IR spectroscopies. The vibrational signatures of crystalline lactose were affected by the presence of water according to QCC results. Water plasticization, involving water insertion, exposure of H-bonding sites, and structure disruption, was accelerated by storage temperature based on Raman and FT-IR spectra analysis. Raman spectra indicated that the crystal formation of lactose was affected by aw and storage temperature. Moreover, the spectral changes assigned in OH group provided useful information for determining the critical aw or temperature when Tg-related molecular mobility occurred in lactose-containing products.

Exploration of sulfate reducing sludge granulation with real domestic sulfate-laden wastewater.

Extending sulfate reducing sludge granulation to real sulfate-laden wastewater was trialed in this study. Two types of reactors, namely sulfate reducing upflow sludge blanket (SRUSB) reactor and continuous stirred tank reactor (CSTR), were used for granulation. Sulfate-reducing granules were observed after 5 months' operation of stepwise hydraulic retention time (HRT) shortening, recirculation cycle adjustment, and air scouring in the SRUSB reactor. Comparatively, granular sludge was achieved in the CSTR after 6 months of cultivation with an average size of 220 µm. With the process of granulation, the HRTs of the two types of reactors were reduced to 2.2 and 2.8 h, respectively for the SRUSB and CSTR reactors. The abundance of sulfate-reducing bacteria (SRB) related genera reached 29.3 and 45% in the SRUSB and CSTR reactors, respectively. Incomplete organic oxidizing SRB were dominant in the CSTR and SRUSB is predominated by complete organic oxidizing SRB, which facilitated a higher organic loading rate. Considering the operating merits, the CSTR is preferable as a pretreatment unit (e.g., for acidification), while the SRUSB reactor can be used for maximum organics removal or sulfide production. Due to the high suspended solids in the influent, full granulation was not achieved by using real sulfate-laden wastewater, and fine particles accumulation in the reactor was also a concern particularly for the long-term operation.

Biosorption Characteristics of Hg(II) from Aqueous Solution by the Biopolymer from Waste Activated Sludge.

The divalent mercury ion (Hg(II)) is one of the most hazardous toxic heavy-metal ions, and an important industrial material as well. It is essential to remove and recover Hg(II) from wastewater before it is released into the environment. In this study, the biosorption characteristics of Hg(II) from aqueous solution by the biopolymer from waste activated sludge (WAS) are investigated. The major components of the biopolymer consisted of proteins, carbohydrates, and nucleic acids. The adsorption kinetics fit for the pseudo-second-order kinetic model, and the adsorption isotherms were well described by Langmuir equation. The adsorption capacity of the biopolymer increased along with rising temperature, and the maximal adsorption capacity was up to 477.0 mg Hg(II)/g biopolymer at 308 K. The infrared spectroscopy analyses showed that the complexation of Hg(II) by the biopolymer was achieved by the functional groups in the biopolymer, including hydroxyl (-OH), amino (-NH2), and carboxylic (-COOH). From the surface morphology, the special reticulate structure enabled the biopolymer to easily capture the metal ions. From the elemental components analyses, a part of Hg(II) ions was removed due to ion exchange with the Na+, K+, and Ca2+, in the biopolymer. Both complexation and ion exchange played key roles in the adsorption of Hg(II) by the biopolymer. These results are of major significance for removal and recovery of Hg(II) from wastewater.

Phosphorus recovery from aqueous solution via a microbial electrolysis phosphorus-recovery cell.

The coming global phosphorus (P) crisis makes P recovery from wastewater become an inevitable choice. Hydroxyapatite (HAP) crystallization is an important approach for P recovery, but its requirements for high alkali and acid are unaffordable. Thus, a microbial electrolysis phosphorus-recovery cell (MEPRC) was developed to cut down the alkali cost via raising the wastewater pH (over 11) in the cathode chamber, and the acid cost via producing acid in the acid-production chamber. HAP was confirmed to be the final recovered products, and P recovery efficiency over 80% was achieved at 24-h operation. To optimize the P recovery performance of this system, the effects of the key factors including applied voltage, P initial concentration and Ca/P ration were investigated. High voltage could promote the rate of P recovery but had slight effect on the eventual recovery efficiency (elevated from 88.5 to 91.1%). High P initial concentration (15.0 mM) could slow down the pH elevation, contributing to the low P recovery efficiency (50.1%) within 24 h. However, prolonging the operation could break the buffering and obtain a satisfactory P recovery efficiency (87.2%) at 36 h. Besides, sufficient calcium ions were favorable to the P recovery. In addition, P recovery cost analyses of the MEPRC indicated that it might be a low-cost technology for P recovery. Moreover, the simultaneously produced acid could be used to neutralize the effluent after P recovery with high pH value. These results demonstrate the feasibility of MEPRC for cost-effective P recovery from wastewater.

Electrochemical acidolysis of magnesite to induce struvite crystallization for recovering phosphorus from aqueous solution.

A novel struvite crystallization method induced by electrochemical acidolysis of cheap magnesite was investigated to recover phosphorus from aqueous solution. Magnesite was confirmed to continuously dissolve in the anolyte whose pH stabilized at about 2. Driven by the electrical field force, over 90% of the released Mg2+ migrated to the cathode chamber via passing through the cation exchange membrane. The pH of the phosphate-containing aqueous solution in the cathode chamber was elevated to the appropriate pH fit for struvite crystallization. The products were identified as struvite crystals by scanning electron microscopy and X-ray diffraction. Increasing the magnesite dosage from 0.83 to 3.33 g L-1 promoted the phosphorus recovery efficiency from 2.2% to 78.3% at 3 d, which was attributed to sufficient Mg2+ supply. Increasing the applied voltage from 3 to 6 V improved the recovery efficiency from 43.6% to 76.4% at 1 d, since the enhanced current density of the electrochemical system markedly accelerated both the magnesite acidolysis and the catholyte pH elevation. The initial catholyte pH between 3 and 5 was found to benefit the phosphorus recovery due to the final catholyte pH fit for the struvite crystallization.

A review of biological sulfate conversions in wastewater treatment.

Treatment of waters contaminated with sulfur containing compounds (S) resulting from seawater intrusion, the use of seawater (e.g. seawater flushing, cooling) and industrial processes has become a challenging issue since around two thirds of the world's population live within 150 km of the coast. In the past, research has produced a number of bioengineered systems for remediation of industrial sulfate containing sewage and sulfur contaminated groundwater utilizing sulfate reducing bacteria (SRB). The majority of these studies are specific with SRB only or focusing on the microbiology rather than the engineered application. In this review, existing sulfate based biotechnologies and new approaches for sulfate contaminated waters treatment are discussed. The sulfur cycle connects with carbon, nitrogen and phosphorus cycles, thus a new platform of sulfur based biotechnologies incorporating sulfur cycle with other cycles can be developed, for the removal of sulfate and other pollutants (e.g. carbon, nitrogen, phosphorus and metal) from wastewaters. All possible electron donors for sulfate reduction are summarized for further understanding of the S related biotechnologies including rates and benefits/drawbacks of each electron donor. A review of known SRB and their environmental preferences with regard to bioreactor operational parameters (e.g. pH, temperature, salinity etc.) shed light on the optimization of sulfur conversion-based biotechnologies. This review not only summarizes information from the current sulfur conversion-based biotechnologies for further optimization and understanding, but also offers new directions for sulfur related biotechnology development.