[Research on therapeutic effect of yangganjiejiu prescription on alcoholic fatty liver and its mechanism].

OBJECTIVE: To research pharmacodynamic effect of Yangganjiejiu prescription on alcoholic fatty liver and its mechanism. METHODS: The alcoholic fatty liver models of rats was set up by feeding high fat diet and alcohol. Sixty male SD rats were randomly divided into 6 groups: normal group, model group, low-dose Yangganjiejiu prescription group (0.7 g/kg), medial-dose group (1.4 g/kg), high-dose group (2.8 g/kg) and positive control drug Dongbao Gantai group, 10 rats were in each group. RESULTS: Yangganjiejiu prescription (1.4 and 2.8 g/kg) could significantly reduce alcoholic fatty liver rats' serum and liver tissues of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol( LDL-C) levels (compared with the model group, P < 0.05) and lower serum free fatty acids (FFA) content (compared with the model group, P < 0.05), and to some extent increase the serum and liver tissues of high-density lipoprotein (HDL-C) levels (but compared with the model group, there was no statistical significance, P > 0.05); It could improve liver function, lower serum AST, ALP and GGT content (compared with the model group, P < 0.05 or P < 0.01), significantly decrease serum and liver tissue MDA (P < 0.01), increase SOD in serum and liver tissue (P < 0.01), alleviate alcoholic fatty on the liver secondary liver pathology, and reduce fatty liver. CONCLUSION: Yangganjiejiu prescription has preventive and therapeutic effect on alcoholic fatty liver. Its mechanism is closely related to resistance to oxidative damage, reducing blood lipid, protecting liver and lowering transaminase.

[Effect of jianpihuashi decoction on rats with hyperuricemia].

OBJECTIVE: To investigate the effect of Jianpihuashi Decoction on rats with hyperuricemia. METHODS: Forty male Sprague-Dawley (SD) rats were randomly divided into four groups: normal, hyperuricemia, Jianpihuashi Decoction and Allopurinol group. After the administration for 0 day, 10 days, 20 days and 30 days, the serum uric acid, creatinine, urea nitrogen and xanthine oxidase (XOD) activity levels were separately detected using the orbital blood. 30 days after the experiment, the rats were anaesthetized by 3% pentobarbital sodium, liver tissue homogenate extracts were used to detect the XOD activity, and histopathological changes in kidney were observed by HE staining. RESULTS: Treatment with Jianpihuashi Decoction for 30 days, the serum uric acid level of rats with hyperuricemia were significantly decreased (P < 0.05). Simultaneously, the XOD activity in the serum and liver tissue homogenate extracts were obviously decreased by the decoction, which had seldom toxic or side effects on kidney. Allopurinol group could significantly decrease the serum uric acid level, but it had seldom pathological injury to kidney at the same time. CONCLUSION: Jianpihuashi Decoction which has seldom pathological injury to kidney can significantly decrease the effect of uric acid by suppressing XOD activity.