[UPLC-Q-TOF-MS analysis of chemical constituents of classical prescription Yiguanjian standard decoction].

An UPLC-Q-TOF-MS method was employed to characterize and classify the chemical components of the standard decoction of Yiguanjian, a classical famous recipe. Chromatographic separation was performed on an Acquity HSS T3(2.1 mm ×100 mm, 1.8 µm) column with a mobile phase of 0.1% formic acid water-0.1% formic acid acetonitrile using gradient elution. The flow rate was 0.4 mL·min~(-1) and the column temperature was 40 ℃. Mass spectrometry was performed on electrospray ionization source(ESI) with positive and negative ion scanning modes. The potential compounds were identified by comparing the reference compounds, analyzing the mass spectrometry data and matching the published articles on Masslynx 4.1 software and SciFinder database. Finally, a total of 113 compounds, including 11 amino acids, 19 terpenoids, 13 phthalides, 11 steroidal saponins, 10 coumarins, 9 alkaloids, 7 flavonoids, 8 phenylethanoid glycosides, 8 organic acids and 17 other categories were identified. The established method systematically and accurately characterized the chemical components in Yiguanjian, which could provide experimental evidences for the subsequent studies on the pharmacodynamical material basis and quality control of Yiguanjian.

The changes of Lp-PLA2 in patients with gestational diabetes and its clinical significance.

ABSTRACT: Gestational diabetes mellitus (GDM) is one of the most common complications of pregnancy and associated with adverse pregnancy outcomes. The aim of this study was to investigate the changes of lipoprotein-associated phospholipaseA2 (Lp-PLA2) level and its correlation with biochemical indexes in patients with GDM.This observational cross-sectional study was performed among 52 GDM and 48 healthy pregnant women. Automatic biochemical analyzer was employed to test the biochemical indexes, including fasting plasma glucose (FPG), Hemoglobin A1c (HbA1c), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). The lipoprotein-associated phospholipaseA2 (Lp-PLA2) level was evaluated by enzyme-linked immunosorbent assay, and homeostatic model assessment for insulin resistance (HOMA-IR) was calculated.The levels of FPG, HbA1c, HOMA-IR, TG, TC and LDL-C were significantly increased while high-density lipoprotein cholesterol (HDL-C) level was significantly decreased in the GDM group when compared with those in the control group. Lp-PLA2 level in maternal blood in the GDM group was significantly higher than that in the control group (199.125 ±â€Š23.494 vs165.825 ±â€Š15.576 ng/mL, P < .05) and logistic regression analysis further confirmed the association of Lp-PLA2 levels with GDM. Furthermore, Lp-PLA2 positively correlated with HOMA-IR, TC, and LDL-C.Our results confirmed the association of Lp-PLA2 with GDM. This broadens our knowledge on the pathophysiology of GDM and provides insights into the development of new targets for the prevention and treatment of GDM.

[Trend of Syphilis Infection among Voluntary Blood Donors in Foshan City from 2008 to 2017].

OBJECTIVE: To investigate the prevalence of syphilis infection in voluntary blood donors in Foshan City, so as to provide reference for improving the infusion blood safety strategy of city. METHODS: A total of 657 382 voluntary blood donors in Foshan City from January 2008 to December 2017 were selected. The blood samples were tested for treponema pallidum. The prevalence trends, the detection rate of treponema pallidum infection and epidemiological data were statistically analyzed. Enzyme-linked immunosorbent assay (ELISA) was performed for antibody screening in blood samples. The two-hole retest was then performed for the samples which were positive in initial test, meanwhile, they were also confirmed by the treponema hemagglutination blood coagulation test (TPHA). RESULTS: First, the annual infection rate of treponema pallidum in Foshan City from January 2008 to December 2017 was 0.43%, 0.40%, 0.41%, 0.43%, 0.50%, 0.32%, 0.36%, 0.32%, 0.37% and 0.32%, respectively. A total of 2502 cases of treponema pallidum infection were detected, the overall infection rate was 0.38%, and the infection rate decreased year by year (x2=23.826, P＜0.05). Second, among the 657 382 samples of voluntary blood donors, 2 502 were showed the positive treponema pallidum antibody (0.38%). In 2015-2017, 791 donors were showed positive treponema pallidum antibody, 676 case out of which were confirmed positive, 0.34% with the positive coincidence rate of 85.46%. Third, this group of 676 syphilis infection cases were male-dominated (0.35%, 577/164 484). The age distribution was 18-53 years old, and the average age was 32 years, among which 35-44 years old accounted for 0.33% (138/41 858). The education level of in donors with treponema pallidom positive mainly ranked at low or lower levels under janior college (0.32%, 571/180 917). The occupation is mainly outside work and company staff (0.39%, 452/115 101). The marital status is mainly unmarried (0.29%, 377/130 905). The place of origin is mainly non-local (0.29%,570/198 063). CONCLUSION: The positive rate of treponema pallidum shows a downward trend in 2008-2017. Among the individuals with comfirmed TP antibody positive in 2015-2017, the non-local, unmarried and low educated males are main population, moreover more than half of them are migrant worker or company employeers.

The value of transvaginal ultrasound in clinical surgical treatment of cesarean scar pregnancy.

The clinical value of transvaginal ultrasound in clinical surgical treatment of cesarean scar pregnancy (CSP) was evaluated. The clinical data of 104 patients with CSP admitted at Tongji Hospital from 2013 to 2016 were collected and analyzed retrospectively, including the patients' age, gestational age, the size of gestational sac or uneven mass, the uterine scar thickness, ß human chorionic gonadotropin (hCG) levels and so on. Of these 104 cases, 30 cases were subjected to laparotomy, 29 cases to laparoscopy, 27 cases to hysteroscopy, 16 cases to ultrasound-guided uterine curettage, and 2 cases to conservative treatment. The transvaginal ultrasound showed that uterine scar thickness and gestational sac or uneven mass size had significant difference (P<0.05) among different surgical methods by comparatively analyzing the patients' data. It was suggested that transvaginal ultrasound may provide the valuable reference for choosing clinical surgical procedures for CSP.