RESUMO
BACKGROUND: Plant height (PH) is an important agronomic trait influenced by a complex genetic network. However, the genetic basis for the variation in PH in Medicago sativa remains largely unknown. In this study, a comprehensive genome-wide association analysis was performed to identify genomic regions associated with PH using a diverse panel of 220 accessions of M. sativa worldwide. RESULTS: Our study identified eight novel single nucleotide polymorphisms (SNPs) significantly associated with PH evaluated in five environments, explaining 8.59-12.27% of the phenotypic variance. Among these SNPs, the favorable genotype of chr6__31716285 had a low frequency of 16.4%. Msa0882400, located proximal to this SNP, was annotated as phosphate transporter 3;1, and its role in regulating alfalfa PH was supported by transcriptome and candidate gene association analysis. In addition, 21 candidate genes were annotated within the associated regions that are involved in various biological processes related to plant growth and development. CONCLUSIONS: Our findings provide new molecular markers for marker-assisted selection in M. sativa breeding programs. Furthermore, this study enhances our understanding of the underlying genetic and molecular mechanisms governing PH variations in M. sativa.
Assuntos
Estudo de Associação Genômica Ampla , Medicago sativa , Polimorfismo de Nucleotídeo Único , Medicago sativa/genética , Fenótipo , Genes de Plantas , Locos de Características Quantitativas/genética , GenótipoRESUMO
RATIONALE: Endovascular embolization has been widely applied in carotid artery aneurysm due to less trauma and simpler procedures than open surgery. Sudden cardiac arrest is a rare event that may cause severe consequences during endovascular embolization. Risk factors of perioperative cardiac arrest include cardiac surgery, younger age, comorbid conditions, and emergency surgery. PATIENT CONCERNS: A 62-year-old male patient had hypertension for 15 years and experienced sudden cardiac arrest of pulseless electrical activity during endovascular embolization. DIAGNOSES: He was diagnosed with a 3.5 × 2.5 mm aneurysm. INTERVENTIONS: Chest compression and effective interventions were given. OUTCOMES: He was resuscitated by cardiopulmonary resuscitation and systematic therapy. LESSONS: This case may provide experience in the management of sudden cardiac arrest during endovascular embolization of a carotid artery aneurysm.
Assuntos
Aneurisma , Doenças das Artérias Carótidas , Embolização Terapêutica , Procedimentos Endovasculares , Masculino , Humanos , Pessoa de Meia-Idade , Aneurisma/cirurgia , Embolização Terapêutica/efeitos adversos , Embolização Terapêutica/métodos , Doenças das Artérias Carótidas/complicações , Doenças das Artérias Carótidas/terapia , Morte Súbita Cardíaca/etiologia , Procedimentos Endovasculares/efeitos adversos , Procedimentos Endovasculares/métodos , Artéria Carótida Interna/cirurgiaRESUMO
Plant filamentation temperature-sensitive H (FtsH) proteins are ATP-dependent zinc proteases that play an important role in regulating abiotic stress adaptions. Here we explore their potential role in abiotic stress tolerance in alfalfa, an important legume crop. Genomic analysis revealed seventeen MsFtsH genes in five clusters, which generally featured conserved domains and gene structures. Furthermore, the expression of MsFtsHs was found to be tightly associated with abiotic stresses, including osmotic, salt and oxidative stress. In addition, numerous stress responsive cis-elements, including those related to ABA, auxin, and salicylic acid, were identified in their promoter regions. Moreover, MsFtsH8 overexpression was shown to confer tolerance to salt and oxidative stress which was associated with reduced levels of reactive oxygen species, and enhanced expression and activity of antioxidant enzymes. Our results highlight MsFtsHs as key factors in abiotic stress tolerance, and show their potential usefulness for breeding alfalfa and other crops with improved yield and stress tolerance.