Numerical studies of manipulation and separation of microparticles in ODEP-based microfluidic chips.

A novel optical-induced dielectrophoresis (ODEP) method employing a pressure-driven flow for the continuous separation of microparticles is presented in this study. By applying alternate current electric field on conductive indium tin oxide substrate and projecting the light geometry into the photoconductive layer, an inhomogeneous electric field is locally induced. The particles experience the dielectrophoretic force when passing through the lighting area, where the strongest electrical field gradient exists. By optimizing the structure of the lighting pattern, a stronger nonuniform electric field gradient is generated which predicts the separation of 1 and 3 µm polystyrene particles. Moreover, the effects of key parameters, including the light pattern geometry, applied voltage, and flow rate, were investigated in this study, leading to the successful sorting of 700 nm and 1 µm particles. To further examine the separation sensitivity and practicability of the proposed ODEP microfluidic method, the isolation of two different types of circulating tumor cells from T-cells and red blood cells are demonstrated, providing a novel method for the manipulation and separation of microparticles and nanoparticles.

Identification and detection of microplastic particles in marine environment by using improved faster R-CNN model.

Microplastics refer to plastic particles measuring less than 5 mm, which has led to serious environmental problem and the detection of these tiny particles is crucial for understanding the corresponding distribution and impact on the marine environment. In this paper, an improved faster region-based convolutional neural network (R-CNN) model was developed for the identification and detection of microplastic particles. In the proposed model, the residual network-50 (ResNet-50) is employed as the backbone with the replacement of the traditional one to enhance the feature extraction capability and the feature pyramid networks (FPN) module is introduced together for solving the multi-scale target detection. By using the improved Faster R-CNN model, the network model performance is enhanced where the average confidence of detecting unique microplastic particles in the marine environment reaches as high as 99%. Moreover, the microparticles mixture was bounded precisely via the predicted bounding boxes without missing detection and wrong detection. In this way, the successful identification of polystyrene microplastic particles from the particles suspension with similar shapes but various conditions of backgrounds, brightness, distributions and object sizes, was achieved by employing the proposed improved Faster R-CNN model, enabling the accurate detection of microplastic particles in marine environment.

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression.

Dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern apple (Malus domestica) production. M26 is a semi-dwarfing rootstock that is used worldwide, but identifying intensive dwarfing rootstock is a major goal of apple breeding programs. Herein, we show that MdWRKY9 mediates dwarfing by directly inhibiting the transcription of the brassinosteroid (BR) rate-limiting synthetase MdDWF4 and reducing BR production. We found that the transcriptional factor MdWRKY9 is highly expressed in all tested dwarfing rootstocks. Transgenic lines of M26 rootstock overexpressing MdWRKY9 exhibit further dwarfing, which resulted from the reduced BR levels and was reversed via exogenous brassinolide treatment. Both an in vivo chromatin immunoprecipitation (ChIP) analysis and an in vitro electrophoretic mobility shift assay (EMSA) indicated that MdWRKY9 binds to the promoter of MdDWF4. Furthermore, MdWRKY9 repressed MdDWF4 expression in stable transgenic apple plants as determined by quantitative PCR. In addition, RNA-interfered expression of MdWRKY9 in transiently transformed apple calli led to a significant increase of MdDWF4, suggesting MdWRKY9 plays a critical role in regulating the expression of MdDWF4. We report a novel dwarfing mechanism in perennial woody plants that involves WRKY-controlled BR production, and present a new dwarfing M26 rootstock for potential applications in apple production.