RESUMO
Turbot (Scophthalmus maximus) is an economically important marine fish cultured in China. In this study, fish in the experimental group were exposed to four temperatures: 15, 20, 25 and 28 °C. Metabolomics analysis and quantitative real-time PCR were used to assess changes in metabolic profiling and gene expression associated with thermal stress. The results showed the levels of heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), blood creatinine and cortisol in S. maximus were all significantly upregulated (P < 0.05), indicating a stress response at 25 °C or higher. Challenge with thermal stress significantly increased expression levels of succinate dehydrogenase (SDH), fructose-1, 6-bisphosphatase (FBPase), malate dehydrogenase (MDH), cytosolic phosphoenolpyruvate carboxykinase (cPEPCK), glucose-6-phosphatase (G6Pase) and aspartate aminotransferase (AST) (P < 0.05). However, there was no effect on the expression levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and mitochondrial phosphoenolpyruvate carboxykinase (mPEPCK). Moreover, high temperature decreased levels of glycogenic amino acids, including histidine, threonine, glutamine, phenylalanine, arginine, serine, tyrosine, methionine and isoleucine. These findings suggest a significant correlation between gene expression and regulation of carbohydrate and amino acid metabolism in heat-stressed S. maximus kidney. In addition, the maintenance of aerobic metabolism and activation of gluconeogenesis appeared to be a critical metabolic strategy in combating heat stress in turbot kidney.
Assuntos
Linguados/fisiologia , Proteínas de Choque Térmico/metabolismo , Resposta ao Choque Térmico/fisiologia , Rim/metabolismo , Metaboloma , Animais , Testes Hematológicos/veterinária , Metabolômica , Reação em Cadeia da Polimerase em Tempo Real/veterináriaRESUMO
The rapid development of modern society has resulted in discharge of large, heavy metal quantities into wetlands that have been continuously accumulating, causing severe pollution. Dajiuhu, located in the Shennongjia Forest District of Hubei Province in China, is a wetland of significant value internationally, serving as a model wetland ecosystem with heightened scientific research value. In this study, 27 surface sediment samples from nine sub-lakes in Dajiuhu were collected in August 2020. The concentrations of Cd, Cr, Cu, Ni, Pb, and Zn in the sediments were determined. The heavy metal occurrence and speciation characteristics were analyzed by an improved BCR (European Community Bureau of Reference) extraction method. Four methods were used to evaluate heavy metals' pollution degree and ecological risk. The possible source of heavy metals was inferred using correlation analysis and principal component analysis. The heavy metal content in the lake sediments of Dajiuhu wetland was from the highest to the lowest concentration as follows: Zn [Formula: see text] Cr [Formula: see text] Ni [Formula: see text] Pb [Formula: see text] Cu [Formula: see text] Cd. The average Cd content exceeded the national nature reserve threshold values, while the other heavy metals measured were below their respective threshold values. However, due to the occurrence of Pb and Cd in different forms, they still pose certain pollution and ecological risk to the lake wetlands. On the other hand, Zn, Cr, Ni, and Cu do not pose an ecological risk in the lakes of the Dajiuhu wetland. The spatial distribution of heavy metal content in the nine sub-lakes did vary significantly. Regarding the heavy metal sources in the lake sediments, Ni, Cr, and Cu originate from natural factors, and Cd and Pb have mainly anthropogenic origins. In contrast, Zn has both natural and anthropogenic origins. This study provides further insights into the study of heavy metal pollution in lake wetlands. It provides a framework and a direction for managing heavy metal pollution in the Dajiuhu wetland.
Assuntos
Metais Pesados , Poluentes Químicos da Água , Áreas Alagadas , Ecossistema , Lagos/análise , Cádmio/análise , Chumbo/análise , Sedimentos Geológicos/análise , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Metais Pesados/análise , Medição de Risco , ChinaRESUMO
Mitogen-activated protein kinases (MAPKs) and heat shock proteins (HSPs) are ubiquitous proteins that are functional mediators in both normal and stressed states of the cell. In this study, we performed heat stress (37 °C) experiments on turbot kidney (TK) cells. Heat stress expression patterns of HSP90, as well as the expression and phosphorylation levels of extracellular-regulated signal kinases (ERKs) and the transcription factor HSF1 and c-Fos, were examined. The results show that heat stress activates ERK1/2 and HSF1, and induces HSP90 gene expression in TK cells. Inhibition of ERK activation attenuates heat stress-induced HSP90 gene expression. The double luciferase reporter gene experiment showed that HSF1 is an important transcription factor for heat-induced HSP90 gene expression. Likewise, c-Fos does not directly regulate the heat-induced expression of HSP90 in turbot kidney cells. To our knowledge, this is the first study to report a signaling pathway that regulates the heat shock response in turbot cells. Our results may facilitate an understanding of the underlying molecular mechanisms of the cellular stress response in marine fish.
Assuntos
Proteínas de Peixes/metabolismo , Linguados/fisiologia , Proteínas de Choque Térmico HSP90/metabolismo , Fatores de Transcrição de Choque Térmico/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Animais , Proteínas de Peixes/genética , Linguados/genética , Proteínas de Choque Térmico HSP90/genética , Resposta ao Choque Térmico , Rim/citologia , Rim/metabolismo , Regulação para CimaRESUMO
We collected evapotranspiration data of Dajiuhu peatland in Shennongjia from 2016 to 2017 with eddy covariance method and estimated the value of crop coefficient (Kc) using FAO56 Penman-Monteith equation and the linear relationship between actual evapotranspiration (ETa) and referenced evapotranspiration (ET0). We analyzed the characteristics of referenced evapotranspiration and its main influencing factors and calculated the crop coefficient of the wetland dominated by Sphagnum. The results showed that the daily averaged ETa were 1.63 and 1.38 mm·d-1 in 2016 and 2017, the daily averaged ET0 were 1.61 and 1.23 mm·d-1 in 2016 and 2017. Environmental factors influencing ET0 included net radiation, air temperature, vapor pressure deficit, wind speed, and relative humidity. The Kc values for the growing seasons of 2016, 2017, and 2016-2017 were 0.95 (R2 of linear regression between ETa and ET0 was 0.96), 1.03 (R2=0.95), and 0.98 (R2=0.95). The Kc values in 2016, 2017, and 2016-2017 were 0.92 (R2=0.94), 0.95 (R2=0.89), and 0.93 (R2=0.92). Kc was effective in the range of 0.92-1.03 for the wetland dominated by Sphagnum. The identified parameters could be widely used in studies on climate change, ecosystem services, and water management in peatlands.
Assuntos
Ecossistema , Transpiração Vegetal , Produtos Agrícolas , Temperatura , Água , VentoRESUMO
To understand the role of 14-3-3 beta/alpha in hypoosmotic regulation of turbot (Scophthalmus maximus), we characterized the 14-3-3 beta/alpha gene and analyzed the tissue distribution and its gene transcriptional patterns in the main expressed tissues under low salt stress. The 14-3-3 beta/alpha cDNA is 892 bp in length, incorporating an ORF of 774 bp with a putative primary structure of 257 residues. The deduced amino acid sequences shared highly conserved structures with other eukaryotes. Quantitative real-time PCR results showed that the 14-3-3 beta/alpha transcripts were widely expressed in various tissues of turbot, with most abundant in the gill (P < .05), to a lesser extent in the kidney, intestine, brain and spleen, and at low levels in the pituitary and other tissues examined. And the expression of turbot 14-3-3 beta/alpha exhibited a trend of increasing first and then decreasing with the time of stress under low salt stress, and the highest value appeared in 12 h (P < .05). After injecting different concentrations of dsRNA, the mRNA expression of 14-3-3 gene decreased significantly during the monitoring period, and the best interference effect was achieved 12 h after injecting 4 µg/g dsRNA. For the first time, the gene was silenced in fish by intramuscular injection of dsRNA. It also provides a new and effective way to study gene function at the individual level. Moreover, the mRNA interference of 14-3-3 beta/alpha would cause changes in the expression of several ion channel proteins, for example, the decrease of Na+-K+-ATPase and Na+-H+-exchanger and the increase of CFTR. As a result, 14-3-3 beta/alpha appears to be an important molecular regulator for osmosensory signal transduction in gill of turbot.