RESUMO
Clarification of solution structure and its modulation in proteins and protein complexes is crucially important to understand dynamical ordering in macromolecular systems. Small-angle x-ray scattering (SAXS) and small-angle neutron scattering (SANS) are among the most powerful techniques to derive structural information. Recent progress in sample preparation, instruments and software analysis is opening up a new era for small-angle scattering. In this review, recent progress and trends of SAXS and SANS are introduced from the point of view of instrumentation and analysis, touching on general features and standard methods of small-angle scattering. This article is part of a Special Issue entitled "Biophysical Exploration of Dynamical Ordering of Biomolecular Systems" edited by Dr. Koichi Kato.
Assuntos
Biologia Computacional , Modelos Biológicos , Difração de Nêutrons , Proteínas/metabolismo , Espalhamento a Baixo Ângulo , Difração de Raios X , Animais , Desenho de Equipamento , Humanos , Cinética , Simulação de Dinâmica Molecular , Difração de Nêutrons/instrumentação , Conformação Proteica , Proteínas/química , Relação Estrutura-Atividade , Difração de Raios X/instrumentaçãoRESUMO
Daptomycin, sold under the trade name CUBICIN, is the first lipopeptide antibiotic to be approved for use against Gram-positive organisms, including a number of highly resistant species. Over the last few decades, a number of studies have tried to pinpoint the mechanism of action of daptomycin. These proposed modes of action often have points in common (e.g. the requirement for Ca2+ and lipid membranes containing a high proportion of phosphatidylglycerol (PG) headgroups), but also points of divergence (e.g. oligomerization in solution and in membranes, membrane perturbation vs. inhibition of cell envelope synthesis). In this study, we investigate how concentration effects may have an impact on the interpretation of the biophysical data used to support a given mechanism of action. Results obtained from small angle neutron scattering (SANS) experiments and molecular dynamics (MD) simulations show that daptomycin oligomerizes at high concentrations (both with and without Ca2+) in solution, but that this oligomer readily falls apart. Photon correlation spectroscopy (PCS) experiments demonstrate that daptomycin causes fusion more readily in DMPC/PG membranes than in POPC/PG, suggesting that the latter may be a better model system. Finally, fluorescence and Förster resonance energy transfer (FRET) experiments reveal that daptomycin binds strongly to the lipid membrane and that oligomerization occurs in a concentration-dependent manner. The combined experiments provide an improved framework for more general and rigorous biophysical studies toward understanding the elusive mechanism of action of daptomycin. This article is part of a Special Issue entitled: Biophysics in Canada, edited by Lewis Kay, John Baenziger, Albert Berghuis and Peter Tieleman.
Assuntos
Cálcio/química , Daptomicina/química , Lipídeos de Membrana/química , Difração de Nêutrons , Espalhamento a Baixo ÂnguloRESUMO
BACKGROUND: Dynamics in haemoglobin from platypus (Ornithorhynchus anatinus), chicken (Gallus gallus domesticus) and saltwater crocodile (Crocodylus porosus) were measured to investigate response of conformational motions on the picosecond time scale to naturally occurring variations in the amino acid sequence of structurally identical proteins. METHODS: Protein dynamics was measured using incoherent quasielastic neutron scattering. The quasielastic broadening was interpreted first with a simple single Lorentzian approach and then by using the Kneller-Volino Brownian dynamics model. RESULTS: Mean square displacements of conformational motions, diffusion coefficients of internal dynamics and residence times for jump-diffusion between sites and corresponding effective force constants (resilience) and activation energies were determined from the data. CONCLUSIONS: Modifications of the physicochemical properties caused by mutations of the amino acids were found to have a significant impact on protein dynamics. Activation energies of local side chain dynamics were found to be similar between the different proteins being close to the energy, which is required for the rupture of single hydrogen bond in a protein. GENERAL SIGNIFICANCE: The measured dynamic quantities showed significant and systematic variations between the investigated species, suggesting that they are the signature of an evolutionary adaptation process stimulated by the different physiological environments of the respective protein.
Assuntos
Hemoglobinas/química , Difração de Nêutrons , Espalhamento a Baixo Ângulo , Jacarés e Crocodilos , Animais , Galinhas , Ornitorrinco , Especificidade da EspécieRESUMO
Halobacterium salinarum is an extreme halophile archaeon with an absolute requirement for a multimolar salt environment. It accumulates molar concentrations of KCl in the cytosol to counterbalance the external osmotic pressure imposed by the molar NaCl. As a consequence, cytosolic proteins are permanently exposed to low water activity and highly ionic conditions. In non-adapted systems, such conditions would promote protein aggregation, precipitation, and denaturation. In contrast, in vitro studies showed that proteins from extreme halophilic cells are themselves obligate halophiles. In this paper, adaptation via dynamics to low-salt stress in H. salinarum cells was measured by neutron scattering experiments coupled with microbiological characterization. The molecular dynamic properties of a proteome represent a good indicator for environmental adaptation and the neutron/microbiology approach has been shown to be well tailored to characterize these modifications. In their natural setting, halophilic organisms often have to face important variations in environmental salt concentration. The results showed deleterious effects already occur in the H. salinarum proteome, even when the external salt concentration is still relatively high, suggesting the onset of survival mechanisms quite early when the environmental salt concentration decreases.
Assuntos
Halobacterium salinarum/genética , Proteoma/metabolismo , Tolerância ao Sal , Estresse Fisiológico , Proteínas Arqueais/genética , Proteínas Arqueais/metabolismo , Halobacterium salinarum/metabolismo , Potássio/metabolismo , Proteoma/genéticaRESUMO
Amphipols are a class of polymeric surfactants that can stabilize membrane proteins in aqueous solutions as compared to detergents. A8-35, the best-characterized amphipol to date, is composed of a polyacrylate backbone with ~35% of the carboxylates free, ~25% grafted with octyl side-chains, and ~40% with isopropyl ones. In aqueous solutions, A8-35 self-organizes into globular particles with a molecular mass of ~40 kDa. The thermal dynamics of A8-35 particles was measured by neutron scattering in the 10-picosecond, 18-picosecond, and 1-nanosecond time-scales on natural abundance and deuterium-labeled molecules, which permitted to separate backbone and side-chain motions. A parallel analysis was performed on molecular dynamics trajectories (Perlmutter et al., Langmuir 27:10523-10537, 2011). Experimental results and simulations converge, from their respective time-scales, to show that A8-35 particles feature a more fluid hydrophobic core, predominantly containing the octyl chains, and a more rigid solvent-exposed surface, made up predominantly of the hydrophilic polymer backbone. The fluidity of the core is comparable to that of the lipid environment around proteins in the center of biological membranes, as also measured by neutron scattering. The biological activity of proteins depends sensitively on molecular dynamics, which itself is strongly dependent on the immediate macromolecular environment. In this context, the characterization of A8-35 particle dynamics constitutes a step toward understanding the effect of amphipols on membrane protein stability and function.
Assuntos
Modelos Químicos , Simulação de Dinâmica Molecular , Difração de Nêutrons/métodos , Polímeros/química , Propilaminas/química , Tensoativos/química , Simulação por Computador , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Conformação Molecular , Solubilidade , Propriedades de Superfície , Temperatura , TermodinâmicaRESUMO
The bacterial flagellar filament is a very large macromolecular assembly of a single protein, flagellin. Various supercoiled states of the filament exist, which are formed by two structurally different conformations of flagellin in different ratios. We investigated the correlation between supercoiling of the protofilaments and molecular dynamics in the flagellar filament using quasielastic and elastic incoherent neutron scattering on the picosecond and nanosecond timescales. Thermal fluctuations in the straight L- and R-type filaments were measured and compared to the resting state of the wild-type filament. Amplitudes of motion on the picosecond timescale were found to be similar in the different conformational states. Mean-square displacements and protein resilience on the 0.1 ns timescale demonstrate that the L-type state is more flexible and less resilient than the R-type, whereas the wild-type state lies in between. Our results provide strong support that supercoiling of the protofilaments in the flagellar filament is determined by the strength of molecular forces in and between the flagellin subunits.
Assuntos
Flagelos/química , Flagelos/metabolismo , Simulação de Dinâmica Molecular , Proteínas Motores Moleculares/química , Proteínas Motores Moleculares/metabolismo , Movimento , Salmonella typhimurium , Cinética , Difração de Nêutrons , Estrutura Secundária de Proteína , Salmonella typhimurium/citologia , TemperaturaRESUMO
The discovery of extreme halophile microorganisms in the Dead Sea, which are specifically dependent on a multimolar salt environment to survive, stimulated major developments in biology and physical chemistry. The minireview focuses on the molecular level. After a brief introduction to the history of halophile studies, protein and nucleic acid solvent interactions and their influence on macromolecular structure stabilization and dynamics are discussed.
Assuntos
Proteínas , Cloreto de Sódio , Água/químicaRESUMO
While the steady-state existence in the size and shape of liquid-ordered microdomains in cell membranes, the so-called "lipid rafts", still remain the subject of debate, glycosphingolipid-cholesterol rich regions in plasma membranes have been considered to have a function as platforms for signaling and sorting. In addition, recent spectroscopic studies show that the interaction between monosialoganglioside and amyloid beta (Aß protein promotes the transition of Aß from the native structure to the cross-beta fold in amyloid aggregates. However, there is few evidence on the dynamics of "lipid rafts" membranes. As the neutron spin-echo (NSE) technique is well known to detect directly slow dynamics of membrane systems in situ, by the combination of NSE and small-angle X-ray scattering we have studied the effect of the interaction between raft-model membrane and amyloid Aß proteins on the structure and dynamics of a large uni-lamellar vesicle (LUV) consisting of monosialoganglioside-cholesterol-phospholipid ternary mixtures as a model of lipid-raft membrane. We have found that the interaction between the Aß proteins and the model membrane at the liquid crystal phase significantly suppresses a bending-diffusion motion with a minor effect on the LUV structure. The present results would suggest a possibility of non-receptor-mediated disorder in signaling through a modulation of a membrane dynamics induced by the association of amyloidogenic peptides on a plasma membrane.
Assuntos
Peptídeos beta-Amiloides/química , Microdomínios da Membrana/química , Peptídeos beta-Amiloides/metabolismo , Colesterol/química , Gangliosídeo G(M1)/química , Microdomínios da Membrana/metabolismo , Ligação Proteica , Espalhamento a Baixo Ângulo , Lipossomas Unilamelares/química , Difração de Raios XRESUMO
Neutron spectroscopy provides experimental data on time-dependent trajectories, which can be directly compared to molecular dynamics simulations. Its importance in helping us to understand biological macromolecules at a molecular level is demonstrated by the results of a literature survey over the last two to three decades. Around 300 articles in refereed journals relate to neutron scattering studies of biological macromolecular dynamics, and the results of the survey are presented here. The scope of the publications ranges from the general physics of protein and solvent dynamics, to the biologically relevant dynamics-function relationships in live cells. As a result of the survey we are currently setting up a neutron Dynamics Data Bank (nDDB) with the aim to make the neutron data on biological systems widely available. This will benefit, in particular, the MD simulation community to validate and improve their force fields. The aim of the database is to expose and give easy access to a body of experimental data to the scientific community. The database will be populated with as much of the existing data as possible. In the future it will give value, as part of a bigger whole, to high throughput data, as well as more detailed studies. A range and volume of experimental data will be of interest in determining how quantitatively MD simulations can reproduce trends across a range of systems and to what extent such trends may depend on sample preparation and data reduction and analysis methods. In this context, we strongly encourage researchers in the field to deposit their data in the nDDB.
Assuntos
Bases de Dados de Compostos Químicos , Simulação de Dinâmica Molecular , Difração de Nêutrons , Biofísica/métodos , Biofísica/organização & administração , Biofísica/tendências , Carboidratos/química , Ácidos Nucleicos/química , Proteínas/químicaRESUMO
The removal of the heme group from myoglobin (Mb) results in a destabilization of the protein structure. The dynamic basis of the destabilization was followed by comparative measurements on holo- (holo-Mb) and apomyoglobin (apo-Mb). Mean-squared displacements (MSD) and protein resilience on the picosecond-to-nanosecond timescale were measured by elastic incoherent neutron scattering. Differences in thermodynamic parameters, MSD, and resilience were observed for both proteins. The resilience of holo-Mb was significantly lower than that of apo-Mb, indicating entropic stabilization by a higher degree of conformational sampling in the heme-bound folded protein. Molecular dynamics simulations provided site-specific information. Averaged over the whole structure, the molecular dynamics simulations yielded similar MSD and resilience values for the two proteins. The mobility of residues around the heme group in holo-Mb showed a smaller MSD and higher resilience compared to the same residue group in apo-Mb. It is of interest that in holo-Mb, higher MSD values are observed for the residues outside the heme pocket, indicating an entropic contribution to protein stabilization by heme binding, which is in agreement with experimental results.
Assuntos
Apoproteínas/química , Simulação de Dinâmica Molecular , Mioglobina/química , Difração de Nêutrons , Animais , Entropia , Heme/química , Cavalos , Estabilidade Proteica , Desdobramento de Proteína , Fatores de Tempo , Temperatura de TransiçãoRESUMO
Base J (ß-D-glucosyl-hydroxymethyluracil) was discovered in the nuclear DNA of some pathogenic protozoa, such as trypanosomes and Leishmania, where it replaces a fraction of base T. We have found a J-Binding Protein 1 (JBP1) in these organisms, which contains a unique J-DNA binding domain (DB-JBP1) and a thymidine hydroxylase domain involved in the first step of J biosynthesis. This hydroxylase is related to the mammalian TET enzymes that hydroxylate 5-methylcytosine in DNA. We have now studied the binding of JBP1 and DB-JBP1 to oligonucleotides containing J or glucosylated 5-hydroxymethylcytosine (glu-5-hmC) using an equilibrium fluorescence polarization assay. We find that JBP1 binds glu-5-hmC-DNA with an affinity about 40-fold lower than J-DNA (~400 nM), which is still 200 times higher than the JBP1 affinity for T-DNA. The discrimination between glu-5-hmC-DNA and T-DNA by DB-JBP1 is about 2-fold less, but enough for DB-JBP1 to be useful as a tool to isolate 5-hmC-DNA. Pre-steady state kinetic data obtained in a stopped-flow device show that the initial binding of JBP1 to glucosylated DNA is very fast with a second order rate constant of 70 µM(-1) s(-1) and that JBP1 binds to J-DNA or glu-5-hmC-DNA in a two-step reaction, in contrast to DB-JBP1, which binds in a one-step reaction. As the second (slower) step in binding is concentration independent, we infer that JBP1 undergoes a conformational change upon binding to DNA. Global analysis of pre-steady state and equilibrium binding data supports such a two-step mechanism and allowed us to determine the kinetic parameters that describe it. This notion of a conformational change is supported by small-angle neutron scattering experiments, which show that the shape of JBP1 is more elongated in complex with DNA. The conformational change upon DNA binding may allow the hydroxylase domain of JBP1 to make contact with the DNA and hydroxylate T's in spatial proximity, resulting in regional introduction of base J into the DNA.
Assuntos
Proteínas de Transporte/química , Glucosídeos/química , Uracila/análogos & derivados , Proteínas de Transporte/metabolismo , Glucosídeos/metabolismo , Cinética , Conformação Molecular , Ligação Proteica , Fatores de Tempo , Uracila/química , Uracila/metabolismoRESUMO
The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.
Assuntos
Proteínas/química , Tensoativos/química , Água/química , Animais , Estrutura Molecular , Mioglobina/química , Solventes/químicaRESUMO
Neutron and X-ray beams in scattering experiments have similar wavelengths and explore the same length scale (~1 Å or 0.1 nm). Data collection and analysis are also broadly similar for both radiation types. There are fundamental differences, however, between the interaction of X-rays and neutrons with matter, which makes them strongly complementary for structural studies in biology. The property of neutrons to distinguish natural abundance hydrogen from its deuterium isotope and the dispersion relation that leads to the energy of ~1 Å neutrons being of the order of thermal energy are well known. They form the basis, respectively, of contrast variation on the one hand and energy-resolved scattering experiments to study macromolecular dynamics-neutron-specific scattering methods on the other. Interestingly, analysis procedures for the structural and dynamics experiments display common aspects that can be expressed as straight-line relationships. These not only act as controls of good sample preparation, but also yield model-free parameters on an absolute scale that provide fundamental information on the structure and dynamics of the system under study.
Assuntos
Difração de Nêutrons/métodos , Espalhamento a Baixo Ângulo , Ácidos Nucleicos/química , Conformação Proteica , Proteínas/química , Difração de Raios X/métodosRESUMO
The recent discovery of extrasolar Earth-like planets that orbit in their habitable zone of their system, and the latest clues of the presence of liquid water in the subsurface of Mars and in the subglacial ocean of Jupiter's and Saturn's moons, has reopened debates about habitability and limits of life. Although liquid water, widely accepted as an absolute requirement for terrestrial life, may be present in other bodies of the solar system or elsewhere, physical and chemical conditions, such as temperature, pressure, and salinity, may limit this habitability. However, extremophilic microorganisms found in various extreme terrestrial environments are adapted to thrive in permanently extreme ranges of physicochemical conditions. This review first describes promising environments for life in the Solar System and the microorganisms that inhabit similar environments on the Earth. The effects of extreme temperatures, salt, and hydrostatic pressure conditions on biomolecules will be explained in some detail, and recent advances in understanding biophysical and structural adaptation strategies allowing microorganisms to cope with extreme physicochemical conditions are reviewed to discuss promising environments for life in the Solar System in terms of habitability.
Assuntos
Exobiologia , Extremófilos , Planeta Terra , Meio Ambiente Extraterreno , PlanetasRESUMO
We developed a novel, to our knowledge, technique for real-time monitoring of subunit exchange in homooligomeric proteins, using deuteration-assisted small-angle neutron scattering (SANS), and applied it to the tetradecamer of the proteasome α7 subunit. Isotopically normal and deuterated tetradecamers exhibited identical SANS profiles in 81% D(2)O solution. After mixing these solutions, the isotope sensitive SANS intensity in the low-q region gradually decreased, indicating subunit exchange, whereas the small-angle x-ray scattering profile remained unchanged confirming the structural integrity of the tetradecamer particles during the exchange. Kinetic analysis of zero-angle scattering intensity indicated that 1), only two of the 14 subunits were exchanged in each tetradecamer and 2), the exchange process involves at least two steps. This study underscores the usefulness of deuteration-assisted SANS, which can provide quantitative information not only on the molecular sizes and shapes of homooligomeric proteins, but also on their kinetic properties.
Assuntos
Deutério/química , Difração de Nêutrons , Complexo de Endopeptidases do Proteassoma/química , Multimerização Proteica , Subunidades Proteicas/química , Espalhamento a Baixo Ângulo , Humanos , Cinética , Modelos Moleculares , Complexo de Endopeptidases do Proteassoma/metabolismo , Estrutura Quaternária de Proteína , Subunidades Proteicas/metabolismoRESUMO
High density lipoprotein (HDL), the carrier of so-called "good" cholesterol, serves as the major athero-protective lipoprotein and has emerged as a key therapeutic target for cardiovascular disease. We applied small angle neutron scattering (SANS) with contrast variation and selective isotopic deuteration to the study of nascent HDL to obtain the low resolution structure in solution of the overall time-averaged conformation of apolipoprotein AI (apoA-I) versus the lipid (acyl chain) core of the particle. Remarkably, apoA-I is observed to possess an open helical shape that wraps around a central ellipsoidal lipid phase. Using the low resolution SANS shapes of the protein and lipid core as scaffolding, an all-atom computational model for the protein and lipid components of nascent HDL was developed by integrating complementary structural data from hydrogen/deuterium exchange mass spectrometry and previously published constraints from multiple biophysical techniques. Both SANS data and the new computational model, the double superhelix model, suggest an unexpected structural arrangement of protein and lipids of nascent HDL, an anti-parallel double superhelix wrapped around an ellipsoidal lipid phase. The protein and lipid organization in nascent HDL envisages a potential generalized mechanism for lipoprotein biogenesis and remodeling, biological processes critical to sterol and lipid transport, organismal energy metabolism, and innate immunity.
Assuntos
Apolipoproteína A-I/química , Lipídeos/química , Lipoproteínas HDL/química , Modelos Moleculares , Apolipoproteína A-I/metabolismo , Transporte Biológico/fisiologia , Humanos , Metabolismo dos Lipídeos/fisiologia , Lipoproteínas HDL/metabolismo , Estrutura Quaternária de Proteína/fisiologia , Estrutura Secundária de Proteína/fisiologiaRESUMO
Recalling that there are currently more than 63,000 structures deposited in the Protein Data Bank, it is time for neutron scatterers studying protein dynamics to show a similar interest in the diversity that is the basis of biology. Sound experimental data that can underpin and complement molecular-dynamics simulations for fundamental research and health applications such as drug design can and should be provided. A proposal is presented to fulfil the two conditions that are required to enable such an approach: (i) the identification of measurable dynamics parameters that are correlated to biological function and activity and (ii) the design of experiments to measure these parameters efficiently with reasonable throughput.
Assuntos
Simulação de Dinâmica Molecular , Difração de Nêutrons , Proteínas/química , Proteínas/metabolismo , Transferência de EnergiaRESUMO
Neutron diffraction from hydrated stacks of natural two-dimensional crystal patches of purple membrane from Halobacterium salinarum was studied as a function of pressure. Measurements in H(2)O and D(2)O permitted the determination of the distribution of water of hydration in the in-plane projection of the membrane. The main experimental difference observed between the samples at 300 MPa and atmospheric pressure was a major reorganization of the hydration around the lipid head groups and protein, associated with a protein conformational change and small reductions in lamellar (stacking) and in-plane lattice spacings, which was consistent with the compressibility of membrane-protein and lipid components.
Assuntos
Pressão Atmosférica , Lipídeos de Membrana/química , Proteínas de Membrana/química , Difração de Nêutrons , Nêutrons , Membrana Purpúrea/química , Deutério/química , Deutério/metabolismo , Halobacterium salinarum/crescimento & desenvolvimento , Halobacterium salinarum/metabolismo , Lipídeos de Membrana/metabolismo , Proteínas de Membrana/metabolismo , Membrana Purpúrea/metabolismo , Temperatura , Água/química , Água/metabolismoRESUMO
There is increasing interest in the contribution of methyl groups to the overall dynamics measured by neutron scattering experiments of proteins. In particular an inflection observed in atomic mean square displacements measured as a function of temperature on high resolution spectrometers (approximately 1 microeV) was explained by the onset of methyl group rotations. By specifically labeling a non-methyl-containing side-chain in a native protein system, the purple membrane, and performing neutron scattering measurements, we here provide direct experimental evidence that the observed inflection is indeed due to methyl group rotations. Molecular dynamics simulations reproduce the experimental data, and their analysis suggests that the apparent transition is due to methyl group rotation entering the finite instrumental resolution of the spectrometer. Methyl group correlation times measured by solid state NMR in the purple membrane, taken from previous work, support the interpretation.
Assuntos
Bacteriorodopsinas/química , Lipídeos/química , Simulação de Dinâmica Molecular , Temperatura , Marcação por Isótopo , Espectroscopia de Ressonância Magnética , Difração de Nêutrons , RotaçãoRESUMO
Water constitutes the intracellular matrix in which biological molecules interact. Understanding its dynamic state is a main scientific challenge, which continues to provoke controversy after more than 50 years of study. We measured water dynamics in vivo in the cytoplasm of Escherichia coli by using neutron scattering and isotope labelling. Experimental timescales covered motions from pure water to interfacial water, on an atomic length scale. In contrast to the widespread opinion that water is 'tamed' by macromolecular confinement, the measurements established that water diffusion within the bacteria is similar to that of pure water at physiological temperature.