Injectable hydrogel made from antler mesenchyme matrix for regenerative wound healing via creating a fetal-like niche.

BACKGROUND: Scar formation and loss of cutaneous appendages are the greatest challenges in cutaneous wound healing. Previous studies have indicated that antler reserve mesenchyme (RM) cells and their conditioned medium improved regenerative wound healing with partial recovery of cutaneous appendages. AIM: To develop hydrogels from the antler RM matrix (HARM) and evaluate the effect on wound healing. METHODS: We prepared the hydrogels from the HARM via enzymatic solubilization with pepsin. Then we investigated the therapeutic effects of HARM on a full-thickness cutaneous wound healing rat model using both local injections surrounding the wound and topical wound application. RESULTS: The results showed that HARM accelerated wound healing rate and reduced scar formation. Also, HARM stimulated the regeneration of cutaneous appendages and blood vessels, and reduced collagen fiber aggregation. Further study showed that these functions might be achieved via creating a fetal-like niche at the wound site. The levels of fetal wound healing-related genes, including Collagen III and TGFß3 treated with HARM were all increased, while the expression levels of Collagen I, TGFß1, and Engrailed 1 were decreased in the healing. Moreover, the number of stem cells was increased in the fetal-like niche created by HARM, which may contribute to the regeneration of cutaneous appendages. CONCLUSION: Overall, we successfully developed an injectable hydrogel made from antler RM matrix for the regenerative repair of full-thickness cutaneous wounds. We uncovered the molecular mechanism of the hydrogels in promoting regenerative wound healing, and thus pave the way for HARM to be developed for the clinic use.

[Spectral radiometric calibration research of Quick Bird digital image].

The present article uses the basic operation of the digital remote image radiometric calibration of the Quickbird with high distinguishing rate, including the physical attribute and the mathematical basement of digital images, the annotation as well as the format of image data. The study makes use of information of spectral radiance from the ground-atmosphere system, which is recorded by the digital remote image of Quick Bird in Honghe area. This dissertation offered the calculation means of radiometric calibration, and changed the pixel digital number into band-integrated radiance. Then, the spectral radiance was calculated. After the radiometric calibration, the Quick Bird image showed the quantitative information of spectral feature from various ground items. Only through the calibration can the Quick Bird image be quantitatively compared and analyzed with other remote sensor images. Thus, the inversion image has the value of application. The significance consists in offering important basic condition for the image amalgamation and better disposal of the special inforation pick-up. This effort also offered spectral information of the ground items for the inversion of the remote image. Therefore, the authors can combine the research of the spectral character of ground items with the establishment of the remote application model in order to quantitatively analyze the ground items.