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The study of exciton polarons has offered profound insights into the many-body interactions between bosonic excitations and their immersed Fermi sea within layered heterostructures. However, little is known about the properties of exciton polarons with interlayer interactions. Here, through magneto-optical reflectance contrast measurements, we experimentally investigate interlayer Fermi polarons for 2s excitons in WSe2/graphene heterostructures, where the excited exciton states (2s) in the WSe2 layer are dressed by free charge carriers of the adjacent graphene layer in the Landau quantization regime. First, such a system enables an optical detection of integer and fractional quantum Hall states (e.g., ν = ±1/3, ±2/3) of monolayer graphene. Furthermore, we observe that the 2s state evolves into two distinct branches, denoted as attractive and repulsive polarons, when graphene is doped out of the incompressible quantum Hall gaps. Our work paves the way for the understanding of the excited composite quasiparticles and Bose-Fermi mixtures.
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The stacking degree of freedom is a crucial factor in tuning material properties and has been extensively investigated in layered materials. The kagome superconductor CsV_{3}Sb_{5} was recently discovered to exhibit a three-dimensional CDW phase below T_{CDW}â¼94 K. Despite the thorough investigation of in-plane modulation, the out-of-plane modulation has remained ambiguous. Here, our polarization- and temperature-dependent Raman measurements reveal the breaking of C_{6} rotational symmetry and the presence of three distinct domains oriented at approximately 120° to each other. The observations demonstrate that the CDW phase can be naturally explained as a 2c staggered order phase with adjacent layers exhibiting a relative π phase shift. Further, we discover a first-order structural phase transition at approximately 65 K and suggest that it is a stacking order-disorder phase transition due to stacking fault, supported by the thermal hysteresis behavior of a Cs-related phonon mode. Our findings highlight the significance of the stacking degree of freedom in CsV_{3}Sb_{5} and offer structural insights to comprehend the entanglement between superconductivity and CDW.
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Luminescent metal-radicals have recently received increasing attention due to their unique properties and promising applications in materials science. However, the luminescence of metal-radicals tends to be quenched after formation of metallo-complexes. It is challenging to construct metal-radicals with highly luminescent properties. Herein, we report a highly luminescent metallo-supramolecular radical cage (LMRC) constructed by the assembly of a tritopic terpyridinyl ligand RL with tris(2,4,6-trichlorophenyl)methyl (TTM) radical and Zn2+. Electrospray ionization-mass spectrometry (ESI-MS), traveling-wave ion mobility-mass spectrometry (TWIM-MS), X-ray crystallography, electron paramagnetic resonance (EPR) spectroscopy, and superconducting quantum interference device (SQUID) confirm the formation of a prism-like supramolecular radical cage. LMRC exhibits a remarkable photoluminescence quantum yield (PLQY) of 65%, which is 5 times that of RL; meanwhile, LMRC also shows high photostability. Notably, significant magnetoluminescence can be observed for the high-concentration LMRC (15 wt % doped in PMMA film); however, the magnetoluminescence of 0.1 wt % doped LMRC film vanishes, revealing negligible spin-spin interactions between two radical centers in LMRC.
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OBJECTIVE: There is an unmet need to improve clinical outcomes for patients with recurrent/metastatic cervical cancer. Checkpoint inhibitors represent a promising treatment strategy. We evaluated the safety and anti-tumor activity of zimberelimab, an anti-programmed cell death protein-1 antibody, in patients with previously treated, recurrent, metastatic cervical cancer. METHODS: This phase II, single-arm, open-label study used a Simon two-stage minimax design. Eligible patients were women aged 18-75 years with programmed death ligand-1-positive recurrent or metastatic cervical cancer that had progressed after first- or subsequent-line chemotherapy (Eastern Cooperative Oncology Group (ECOG) performance status 0-1). Patients received intravenous zimberelimab (240 mg every 2 weeks) for 2 years until disease progression, intolerable adverse effects, or withdrawal from the study. The primary endpoint was objective response rate assessed per Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1, by an independent review committee. RESULTS: A total of 105 patients were enrolled. Median age was 51 (range, 31-75) years; 63.8% had an ECOG performance status of 1. The median number of previous treatment lines was 1 (range, 1-4). Median follow-up was 16.9 (range, 16.3-18.4) months. The objective response rate was 27.6%, and the disease control rate was 55.2%. Median duration of response was not reached. Median overall survival was 16.8 months, and median progression-free survival was 3.7 months. The incidence of treatment-related adverse events of any grade was 78.1%, of which the most common were hypothyroidism (26.7%) and anemia (19.0%). CONCLUSION: Zimberelimab monotherapy demonstrated durable anti-tumor activity and an acceptable safety profile in patients with cervical cancer. CLINICAL TRIAL REGISTRATION: NCT03972722.
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Neoplasias do Colo do Útero , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Anticorpos Monoclonais/uso terapêutico , Anticorpos Monoclonais Humanizados/efeitos adversos , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Inibidores de Checkpoint Imunológico/uso terapêutico , Recidiva Local de Neoplasia/tratamento farmacológico , Neoplasias do Colo do Útero/tratamento farmacológicoRESUMO
A quantum spin liquid is an exotic quantum state of matter in which spins are highly entangled and remain disordered down to zero temperature. Such a state of matter is potentially relevant to high-temperature superconductivity and quantum-information applications, and experimental identification of a quantum spin liquid state is of fundamental importance for our understanding of quantum matter. Theoretical studies have proposed various quantum-spin-liquid ground states, most of which are characterized by exotic spin excitations with fractional quantum numbers (termed 'spinons'). Here we report neutron scattering measurements of the triangular-lattice antiferromagnet YbMgGaO4 that reveal broad spin excitations covering a wide region of the Brillouin zone. The observed diffusive spin excitation persists at the lowest measured energy and shows a clear upper excitation edge, consistent with the particle-hole excitation of a spinon Fermi surface. Our results therefore point to the existence of a quantum spin liquid state with a spinon Fermi surface in YbMgGaO4, which has a perfect spin-1/2 triangular lattice as in the original proposal of quantum spin liquids.
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Ferroelectric (FE) distortions in a metallic material were believed to be experimentally inaccessible because itinerant electrons would screen the long-range Coulomb interactions that favor a polar structure. It has been suggested by Anderson and Blount [P. W. Anderson, E. I. Blount, Phys. Rev. Lett. 14, 217-219 (1965)] that a transition from paraelectric phase to FE phase is possible for a metal if, in the paraelectric phase, the electrons at the Fermi level are decoupled from the soft transverse optical phonons, which lead to ferroelectricity. Here, using Raman spectroscopy combined with magnetotransport measurements on a recently discovered FE metal LiOsO3, we demonstrate active interplay of itinerant electrons and the FE order: Itinerant electrons cause strong renormalization of the FE order parameter, leading to a more gradual transition in LiOsO3 than typical insulating FEs. In return, the FE order enhances the anisotropy of charge transport between parallel and perpendicular to the polarization direction. The temperature-dependent evolution of Raman active in-plane 3Eg phonon, which strongly couples to the polar-active out-of-the-plane A2u phonon mode in the high-temperature paraelectric state, exhibits a deviation in Raman shift from the expectation of the pseudospin-phonon model that is widely used to model many insulating FEs. The Curie-Weiss temperature (θ ≈ 97 K) obtained from the optical susceptibility is substantially lower than T s, suggesting a strong suppression of FE fluctuations. Both line width and Fano line shape of 3Eg Raman mode exhibit a strong electron-phonon coupling in the high-temperature paraelectric phase, which disappears in the FE phase, challenging Anderson/Blount's proposal for the formation of FE metals.
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Underwater noise produced by rainfall is an important component of underwater ambient noise. For example, the existence of rainfall noise causes strong disturbances to sonar performance. The underwater noise produced by a single raindrop is the basis of rainfall noise. Therefore, it is necessary to study the associated underwater noise when drops strike the water surface. Previous research focused primarily on the sound pressure and frequency spectrum of underwater noise from single raindrops, but the study on its sound energy is insufficient. The purpose of this paper is to propose a method for predicting the acoustic energy generated by raindrops of any diameter. Here, a formula was derived to calculate the underwater sound energy radiated by single raindrops based on a dipole radiation pattern. A series of experiments were conducted to measure the underwater sound energy in a 15 m × 9 m × 6 m reverberation tank filled with tap water. The analysis of the acoustic energy characteristics and conversion efficiency from kinetic to acoustic energy helped develop the model to predict the average underwater sound energy radiated by single raindrops. Using this model, the total underwater sound energy of all raindrops during a rainfall event can be predicted based on the drop size distribution.
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The fundamental principles of electrodynamics allow an electron carrying both electric monopole (charge) and magnetic dipole (spin) but prohibit its magnetic counterpart. Recently, it was predicted that the magnetic "monopoles" carrying emergent magnetic charges in spin ice systems can induce electric dipoles. The inspiring prediction offers a novel way to study magnetic monopole excitations and magnetoelectric coupling. However, no clear example has been identified up to now. Here, we report the experimental evidence for electric dipoles induced by magnetic monopoles in spin frustrated Tb_{2}Ti_{2}O_{7}. The magnetic field applied to pyrochlore Tb_{2}Ti_{2}O_{7} along the [111] direction, brings out a "3-in-1-out" magnetic monopole configuration, and then induces a subtle structural phase transition at H_{c}â¼2.3 T. The transition is made evident by the nonlinear phonon splitting under magnetic fields and the anomalous crystal-field excitations of Tb^{3+} ions. The observations consistently point to the displacement of the oxygen O^{''} anions along the [111] axis which gives rise to the formation of electric dipoles. The finding demonstrates that the scenario of magnetic monopole having both magnetic charge and electric dipole is realized in Tb_{2}Ti_{2}O_{7} and sheds light into the coupling between electricity and magnetism of magnetic monopoles in spin frustrated systems.
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Fluopyram, as a reasonably good fungicide and nematicide, is widely used to control agricultural pests worldwide. However, its effects on soil microbial communities and plant growth remain controversial. Therefore, in this study, we investigated the effects of three concentrations (0.5, 1.5, and 5.0â¯mg/kg) of the fluopyram (Lufuda 41.7% a.i., suspension concentrate, SC) on the pepper rhizosphere microorganisms and pepper seedlings growth in a plant growth room. Moreover, we also investigated the dissipation of fluopyram in the soil, pepper roots, and leaves across a time interval of 45 days. The results showed that fluopyram application increased the number of pepper rhizosphere phosphate (P)-solubilizing bacteria, the abundance of nitrogen (N)-fixing nifH genes, and the pepper seedling growth. The results of terminal restriction fragment length polymorphism (T-RFLP) analysis demonstrated that fluopyram did not alter rhizosphere bacterial community structure and diversity. However, fluopyram did increase the relative abundances of 138 bp and 400 bp T-RFs closely representing Bacillus and Rhizobium genera that were known as efficient plant growth promoting bacteria with P-solubilization and N-fixation properties. Corresponding to the increase of plant growth and beneficial microbes, the half-lives of fluopyram in soil and plant tissues also decreased that nevertheless suggested the role of plant-microbe interactions in the faster removal of fluopyram after application. Our results suggest that short-lived and easily degradable pesticides may have less toxicological effects on soil health while their judicious use may reshape plant-microbe interactions in favor of the plant growth.
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Benzamidas/farmacologia , Capsicum/efeitos dos fármacos , Fungicidas Industriais/farmacologia , Bactérias Fixadoras de Nitrogênio/crescimento & desenvolvimento , Fosfatos/química , Piridinas/farmacologia , Microbiologia do Solo , Agricultura , Capsicum/crescimento & desenvolvimento , Fixação de Nitrogênio , Raízes de Plantas/química , Rizosfera , Solo/químicaRESUMO
We report a dramatic change in the intensity of a Raman mode with applied magnetic field, displaying a gigantic magneto-optical effect. Using the nonmagnetic layered material MoS2 as a prototype system, we demonstrate that the application of a magnetic field perpendicular to the layers produces a dramatic change in intensity for the out-of-plane vibrations of S atoms, but no change for the in-plane breathing mode. The distinct intensity variation between these two modes results from the effect of field-induced broken symmetry on Raman scattering cross-section. A quantitative analysis on the field-dependent integrated Raman intensity provides a unique method to precisely determine optical mobility. Our analysis is symmetry-based and material-independent, and thus the observations should be general and inspire a new branch of inelastic light scattering and magneto-optical applications.
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The herbicide "fomesafen" causes phytotoxicity to the rotational wheat crop and may reduce its yield. Considering that biochar may improve remediation and biophysical conditions of the contaminated soil environments to benefit plant growth. Here, we investigated the impacts of three levels of the wheat straw-derived biochar (1%, 2%, and 4% (w/w)) on growth, physiological properties, and rhizosphere microbial communities of the wheat (Triticum aestivum) seedlings under the fomesafen stress using high-throughput sequencing. The results showed that biochar amended into soil significantly reduced the uptake of wheat to fomesafen and thereby eliminate its toxicity to wheat seedlings. Moreover, biochar increased the abundance and diversity of plant beneficial bacterial and fungal taxa in the rhizosphere of wheat seedlings. Compared with the three addition amounts, amendment with 2% of biochar has the best effects to reduce the toxicity of fomesafen on wheat seedlings and maintain the balance of soil microbial community structure in soil contaminated with fomesafen (1.0â¯mgâ¯kg-1). Overall, our results suggest that the level of biochar application influences the structure and diversity of soil microbiome (and mycobiome) and plant performance under abiotic stress conditions.
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Benzamidas/toxicidade , Carvão Vegetal , Herbicidas/toxicidade , Desenvolvimento Vegetal , Microbiologia do Solo , Triticum/crescimento & desenvolvimento , Bactérias , Fungos , Microbiota , Rizosfera , Poluentes do Solo/toxicidade , Triticum/efeitos dos fármacosRESUMO
BACKGROUND: The present study evaluated the clinical outcomes and safety of expansive open-door laminoplasty, when securing with C4 - C6 lateral mass screw and fusion. METHODS: A total of 110 patients with cervical spondylotic myelopathy (CSM) were enrolled. There were 88 male and 22 female, with mean age at 60.55 ± 10.95 years. All of the patients underwent expansive open-door laminoplasty with unilateral or bilateral C4-6 lateral mass screws fixation and fusion. Clinical data, including age, gender, operation-related information, pre- and post-operation Japanese Orthopedic Association (JOA) scores, and cervical curvatures were collected. RESULTS: The mean follow-up time of the cohort was 13.61 ± 9.53 months. Among the 110 patients, 33 of them were allocated to Unilateral group, and 77 of them were in Bilateral group. The mean JOA score of the 110 patients before surgery was 10.07 ± 2.39, and the score was improved significantly to 12.85 ± 2.45 after surgery. There were no reported cases of neurological deterioration or symptom worsening. Patients in both the Unilateral group and Bilateral groups had significant improvement of JOA scores. Among all patients, the most frequently observed complications were axial symptoms (n = 7). The average preoperative cervical curvature among all patients was 15.17 ± 5.26, and the post-surgery curvature was 14.41 ± 4.29. Similar observations were found between Unilateral and Bilateral groups. CONCLUSION: The modified surgical approach provided satisfactory clinical outcome in patients with CSM. The unilateral and bilateral fixation appeared to provide similar outcomes, in terms of cervical curvature maintenance and improvement of clinical symptoms. However, the examination of the exact differences between the two fixation methods await further biomechanical studies.
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Vértebras Cervicais/cirurgia , Laminoplastia/métodos , Fusão Vertebral/métodos , Espondilose/cirurgia , Idoso , Parafusos Ósseos , Feminino , Humanos , Laminoplastia/efeitos adversos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Estudos Prospectivos , Fusão Vertebral/efeitos adversos , Resultado do TratamentoRESUMO
We apply moderate-high-energy inelastic neutron scattering (INS) measurements to investigate Yb^{3+} crystalline electric field (CEF) levels in the triangular spin-liquid candidate YbMgGaO_{4}. Three CEF excitations from the ground-state Kramers doublet are centered at the energies âω=39, 61, and 97 meV in agreement with the effective spin-1/2 g factors and experimental heat capacity, but reveal sizable broadening. We argue that this broadening originates from the site mixing between Mg^{2+} and Ga^{3+} giving rise to a distribution of Yb-O distances and orientations and, thus, of CEF parameters that account for the peculiar energy profile of the CEF excitations. The CEF randomness gives rise to a distribution of the effective spin-1/2 g factors and explains the unprecedented broadening of low-energy magnetic excitations in the fully polarized ferromagnetic phase of YbMgGaO_{4}, although a distribution of magnetic couplings due to the Mg/Ga disorder may be important as well.
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The role of exogenous salicylic acid (SA) in protecting wheat plants (Triticum aestivum) from contamination by the insecticide chlorpyrifos was investigated in this study. The wheat plants were grown in soils with different concentrations (5, 10, 20, and 40mgkg-1) of chlorpyrifos. When the third leaf emerged, the wheat leaves were sprayed with 1, 2, 4, 8, and 16mgL-1 of SA once a day for 6 days. The results showed that wheat exposed to higher concentrations of chlorpyrifos (≥20mgkg-1) caused declines in growth and chlorophyll content and altered the activities of a series of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). Interestingly, treatments with different concentrations of SA mitigated the stress generated by chlorpyrifos and improved the measured parameters to varying degrees. Furthermore, a reverse transcription and quantitative PCR experiment revealed that the activities of SOD and CAT can be regulated by their target gene in wheat when treated with SA. We also found that SA is able to block the accumulation of chlorpyrifos in wheat. However, the effect of SA was related to its concentration. In this study, the application of 2mgL-1 of SA had the greatest ameliorating effect on chlorpyrifos toxicity in wheat plants.
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Clorpirifos/toxicidade , Inseticidas/toxicidade , Ácido Salicílico/farmacologia , Triticum/efeitos dos fármacos , Antioxidantes/farmacologia , Ascorbato Peroxidases/metabolismo , Catalase/metabolismo , Clorofila/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Peroxidase/metabolismo , Peroxidases/metabolismo , Folhas de Planta/efeitos dos fármacos , Solo/química , Poluentes do Solo/toxicidade , Superóxido Dismutase/metabolismoRESUMO
The recent renaissance of black phosphorus (BP) as a two-dimensional (2D) layered material has generated tremendous interest, but its unique structural characters underlying many of its outstanding properties still need elucidation. Here we report Raman measurements that reveal an ultralow-frequency collective compression mode (CCM) in BP, which is unprecedented among similar 2D layered materials. This novel CCM indicates an unusually strong interlayer coupling, and this result is quantitatively supported by a phonon frequency analysis and first-principles calculations. Moreover, the CCM and another branch of low-frequency Raman modes shift sensitively with changing number of layers, allowing an accurate determination of the thickness up to tens of atomic layers, which is considerably higher than previously achieved by using high-frequency Raman modes. These findings offer fundamental insights and practical tools for further exploration of BP as a highly promising new 2D semiconductor.
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Muon spin relaxation (µSR) experiments on single crystals of the structurally perfect triangular antiferromagnet YbMgGaO_{4} indicate the absence of both static long-range magnetic order and spin freezing down to 0.048 K in a zero field. Below 0.4 K, the µ^{+} spin relaxation rates, which are proportional to the dynamic correlation function of the Yb^{3+} spins, exhibit temperature-independent plateaus. All these µSR results unequivocally support the formation of a gapless U(1) quantum spin liquid ground state in the triangular antiferromagnet YbMgGaO_{4}.
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Nonylphenol polyethoxylates (NPEOs) are a group of surfactants that are widely used in industrial and household products and often detected in the environment. The metabolite of NPEOs, named nonylphenol (NP), has proven to be an endocrine disruptor, and its environmental behavior and eco-toxicity have been widely investigated in previous studies. However, to the best of our knowledge, insight into the toxicity differences of NP and NPEOs on important crops remains limited. Therefore, this study investigated the comparative toxicity of NP, nonylphenol-4-ethoxylate (NP4EO), and nonylphenol-10-ethoxylate (NP10EO) on wheat seedlings using hydroponic experiments. The results indicated that NP is most toxic to wheat followed by NP4EO, and NP10EO is the least toxic to wheat. The adverse effects of NP on wheat were observed for all the tested parameters including germination, shoot length, root length, chlorophyll, lipid peroxidation, and enzymatic activities. To gain insight into the molecular response, we analyzed the transcript abundance of SOD-Cu/Zn and CAT with NP, NP4EO, and NP10EO exposure using quantitative real-time PCR. The data revealed that both genes exhibited up- or down-regulated expression patterns that were consistent with the activities of the two enzymes. This result further conformed that NP is most toxic to wheat plants.
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Fenóis/toxicidade , Éteres Fenílicos/toxicidade , Polietilenoglicóis/toxicidade , Plântula/efeitos dos fármacos , Tensoativos/toxicidade , Triticum/efeitos dos fármacos , Catalase/genética , Catalase/metabolismo , Clorofila/metabolismo , Disruptores Endócrinos/toxicidade , Germinação/efeitos dos fármacos , Hidroponia , Peroxidação de Lipídeos/efeitos dos fármacos , Raízes de Plantas/efeitos dos fármacos , Brotos de Planta/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Triticum/crescimento & desenvolvimento , Triticum/metabolismoRESUMO
YbMgGaO4, a structurally perfect two-dimensional triangular lattice with an odd number of electrons per unit cell and spin-orbit entangled effective spin-1/2 local moments for the Yb(3+) ions, is likely to experimentally realize the quantum spin liquid ground state. We report the first experimental characterization of single-crystal YbMgGaO4 samples. Because of the spin-orbit entanglement, the interaction between the neighboring Yb(3+) moments depends on the bond orientations and is highly anisotropic in the spin space. We carry out thermodynamic and the electron spin resonance measurements to confirm the anisotropic nature of the spin interaction as well as to quantitatively determine the couplings. Our result is a first step towards the theoretical understanding of the possible quantum spin liquid ground state in this system and sheds new light on the search for quantum spin liquids in strong spin-orbit coupled insulators.
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PURPOSE: Postoperative cognitive dysfunction (POCD) characterized as the decline of memory and executive function after major surgery is not well illustrated. The aim of this study is to discover whether inflammatory cytokines and urinary trypsin inhibitor (uTi) contribute to the development of POCD. METHOD: Sixty-three patients undergoing lumber discectomy and 47 age-matched control volunteers were involved in this study. The level of C-reaction protein (CRP) and uTi/urine creatinine (Ucr) was measured by immunoturbidimetry and enzyme-inhibition assay, respectively. Meanwhile, ELISA was involved to detect the level of IL-6, IL-10, MMP-9 in serum. Montreal Cognitive Assessment (MoCA) test was used to determine the cognitive decline of the patients and age-matched controls. RESULT: In POCD group, the level of IL-6, IL-10, CRP, MMP-9 in serum and uTi /Ucr in urine was significantly higher than that in the group without POCD. The POCD was more frequently observed in elderly group than in the middle-aged group (43.75% versus 19.35%, p = 0.038). After logistic regression analysis adjusted by the age, only serum CRP at 72 h postoperation and urinary uTi /Ucr at 24 h postoperation were the independent risk factors of POCD. CONCLUSION: Age-related increasing proinflammatory postoperation may result in higher occurrence of POCD in the elderly. Additionally, patients with extremely high concentrations of CRP in serum at 72 h postoperation and uTi /Ucr in urine at 24 h postoperation are more likely to experience POCD, especially in the elderly.
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Envelhecimento/sangue , Proteína C-Reativa/metabolismo , Transtornos Cognitivos/sangue , Transtornos Cognitivos/etiologia , Glicoproteínas/sangue , Complicações Pós-Operatórias/fisiopatologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Feminino , Humanos , Interleucina-10/sangue , Interleucina-6/sangue , Masculino , Metaloproteinase 9 da Matriz/sangue , Entrevista Psiquiátrica Padronizada , Pessoa de Meia-Idade , Testes NeuropsicológicosRESUMO
To study the induced resistance in apple against Valsa mali var. mali (Vmm), a Vmm-apple callus interaction system was developed to evaluate the induced resistance of an attenuated Vmm strain LXS081501 against further infection by a virulent Vmm strain LXS080601. The infection index was up to 97.32 for apple calli inoculated with LXS080601 alone at 15 days after inoculation whereas it was only 41.84 for calli pretreated with LXS081501 followed by LXS080601 inoculation. In addition, the maximum levels of free proline, soluble sugar, and protein in calli treated with LXS081501 plus LXS080601 were 2.14 to 3.47 times higher than controls and 1.42 to 1.75 times higher than LXS080601 treatment. The activities of defense-related enzymes such as phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), and catalase (CAT) as well as ß-1,3-glucanase and chitinase in apple calli inoculated with LXS080601 alone or LXS081501 plus LXS080601 increased significantly 24 hai and peaked from 48 to 120 hpi. However, in the latter treatment, the maximum enzyme activities were much higher and the activities always maintained much higher levels than control during the experimental period. These results suggested the roles of osmotic adjustment substances and defense-related enzymes in induced resistance.