Construction and evaluation of an antioxidant synergistic system containing vitamin C and vitamin E.

AIMS: The aim of this study is to develop a liposome that could exert unparalleled antioxidant effects. In the present study, a vitamin C (VC)/vitamin E (VE)-co-loaded multivesicular liposome (VCVE-MVL) was constructed. METHODS: Vitamins were encapsulated in soybean phosphatidylcholine (SPC) and cholesterol (CHO) by multi-emulsification method. The concentration of VC was determined by Fast Blue method. The concentration of VE was determined by high performance liquid chromatography (HPLC). Vitamin antioxidant capacity in vitro and in vivo was determined using ß-carotene bleaching. RESULTS: VCVE-MVL with particle diameter of 848.55 ± 0.29 nm and SPAN of 0.16 ± 0.11 were obtained. The encapsulation efficiency of VC reached 48.51% (w/w)±0.15. Compared with VC/VE solution, VCVE-MVL had a higher permeation efficiency. In addition, the in vitro and ex-vivo antioxidant tests verified the adequate antioxidant activity of VCVE-MVL. CONCLUSIONS: In conclusion, the antioxidant synergistic system we constructed and demonstrated its potential applications in the cosmetics industry.

Fingerprint combining with quantitative analysis of multi-components by single marker for quality control of Chenxiang Huaqi tablets.

BACKGROUND: Chenxiang Huaqi tablets (CXHQTs) are a traditional Chinese medicine (TCM) commonly used to treat stomach-related diseases. Currently, the ministerial standards do not provide detailed guidance and regulations on the content determination of CXHQTs, and the reported studies only use individual active components as indicators for determining effective ingredients. OBJECTIVE: The present study aimed to propose a methodology for quality control of CXHQTs based on high-performance liquid chromatography (HPLC) fingerprinting combined with the quantitative analysis of multi-components by single marker (QAMS) method. METHODS: HPLC method was used to determine seven active ingredients and performed fingerprint analysis of CXHQTs. To further process chemometric assessment, technical analysis-model including similarity analysis (SA), hierarchical clustering analysis (HCA), principal components analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA) was set up to differentiate and classify the 20 batches of samples. RESULTS: After comparing the results of QAMS method with the external standard method (ESM), we found there is no significant difference. Besides, the fingerprint of CXHQT was also established. CONCLUSION: HPLC fingerprint combined with the QAMS could be an efficient and selective analysis technique to achieve a qualitative and quantitative evaluation of executing quality processes.

[Quality evaluation of Chenxianghuaqi tablets by gas chromatographic fingerprint of volatile components].

A gas chromatographic fingerprint combined with chemical pattern recognition was successfully developed and applied to assess the quality consistency of 20 Chenxianghuaqi tablets. Volatile components from the 20 Chenxianghuaqi tablets were extracted with ethanol under ultrasonic conditions. n-Octadecane was used as the internal standard to calculate the amounts of the three main components and to confirm the relative peak areas of the other components. Gas chromatographic fingerprints of the 20 Chenxianghuaqi tablets were established. There were 11 common peaks in the fingerprints, and the similarity of each batch of samples was obtained. Ten common peaks were identified by gas chromatography-mass spectrometry, with reference comparison. The obtained fingerprints were used for the chemical pattern recognition, including hierarchical cluster analysis and the principal component analysis. Different batches of Chenxianghuaqi tablets could be differentiated effectively, and major markers that led to differences among the sample batches were identified. The method proposed in this study is comprehensive and reliable, and it can be used as a valuable reference to evaluate and control the quality of Chenxianghuaqi tablets.