Ultrathin endoscope equipped with ultrasonic miniprobe for upper GI US in a porcine model.

BACKGROUND AND AIMS: Ultrathin EGD (UT-EGD) is an ideal tool for unsedated upper GI examination and pediatric gastroenterology but is rarely competent for EUS miniprobe (EUS-MP). We developed a UT-EGD US method (UT-EUS) and verified its clinical application value through animal experiments. METHODS: Five Bama miniature pigs were selected. Using an acoustic medium, we performed US on the duodenum, stomach, and esophagus, respectively, with conventional 20-MHz EUS miniprobe (EUS-MP-20), 20-MHz UT-EUS (UT-EUS-20), and 30-MHz UT-EUS (UT-EUS-30). The times to acquire 5 consecutive stable US images, number of identifiable wall layers, and quality and penetration depth of the images were recorded. RESULTS: No significant differences were found in the time required to obtain images between EUS-MP-20 and UT-EUS-20 at each site (P > .05). UT-EUS-30 showed more wall levels than UT-EUS-20 (P < .05). No significant differences were noted between EUS-MP-20 and UT-EUS-20 in imaging quality and penetration depth (P > .05). CONCLUSIONS: The UT-EUS is easy to use with a satisfactory image quality and has potential clinical application value.

Deciphering the multifaceted roles and clinical implications of 2-hydroxyglutarate in cancer.

Increasing evidence indicates that 2-hydroxyglutarate (2HG) is an oncometabolite that drives tumour formation and progression. Due to mutations in isocitrate dehydrogenase (IDH) and the dysregulation of other enzymes, 2HG accumulates significantly in tumour cells. Due to its structural similarity to α-ketoglutarate (αKG), accumulated 2HG leads to the competitive inhibition of αKG-dependent dioxygenases (αKGDs), such as KDMs, TETs, and EGLNs. This inhibition results in epigenetic alterations in both tumour cells and the tumour microenvironment. This review comprehensively discusses the metabolic pathways of 2HG and the subsequent pathways influenced by elevated 2HG levels. We will delve into the molecular mechanisms by which 2HG exerts its oncogenic effects, particularly focusing on epigenetic modifications. This review will also explore the various methods available for the detection of 2HG, emphasising both current techniques and emerging technologies. Furthermore, 2HG shows promise as a biomarker for clinical diagnosis and treatment. By integrating these perspectives, this review aims to provide a comprehensive overview of the current understanding of 2HG in cancer biology, highlight the importance of ongoing research, and discuss future directions for translating these findings into clinical applications.

The Effect of Beautification Strategies on Mating Popularity Judged by Men from the People's Republic of China.

Contemporary women frequently employ beautification strategies. The impact of such strategies, such as plastic surgery, on mating popularity in different mate contexts remains unclear. To investigate this issue, the current study conducted two experiments. In Experiment 1, beautification strategies were manipulated using three images of the same female with different conditions (natural, makeup, and plastic surgery). The results indicated that when the beautification strategies were not informed, surgical-enhanced and makeup targets were perceived as significantly more attractive, loyal, and popular among potential mates than natural targets. However, when participants were informed of the beautification strategies, both natural and makeup targets showed a significant increase in perceived loyalty and mating popularity. In contrast, surgically enhanced targets saw a reduction in these dimensions. Experiment 2 aimed to reduce the confounding effect of facial attractiveness by using vignettes. The results indicated that the mating popularity of natural targets was significantly higher than that of makeup or surgically enhanced targets, with surgically enhanced targets being the least popular. Moreover, the results revealed the mediating role of perceived loyalty in the impact of beautification strategies on long-term mating popularity. This study sheds light on the potential stigmatization and negative bias toward beautification strategies in the mating market. Additionally, it provides guidance for women who intend to enhance their mate popularity through plastic surgery.

Unperceived, the impact of information gap design plateware on food waste.

Curiosity is a powerful motivator of behaviour. Although there have been some studies pertaining to the application of curiosity in the realm of food, research examining the potential to influence consumer food waste behaviour through the induction of curiosity is lacking. This study conducted two onsite dining experiments to explore the role and mechanism of curiosity in reducing food waste in a real dining environment by utilising an information gap design in tableware to induce participants' curiosity. Experiment 1 investigated the differences in food waste between participants using bowls with an information gap design and those using bowls with no information gap (blank bowls). Experiment 2 further controlled for other variables that could potentially influence the outcomes between bowls with and without information gaps; the latter displayed complete text externally. The results of both experiments consistently demonstrated a significant reduction in participants' food waste when utilising utensils with an information gap design. Moreover, we conducted an exploratory analysis combining these two experiments to examine the mediating mechanisms involved. Furthermore, the exploratory analysis suggested the mediating mechanism of curiosity elicited by the information gap design, ultimately leading to a decrease in food waste. This study presents a potential avenue for a simple and innovative approach for mitigating food waste.

Dual-functional significance of ATM-mediated phosphorylation of spindle assembly checkpoint component Bub3 in mitosis and the DNA damage response.

Both the DNA damage response (DDR) and the mitotic checkpoint are critical for the maintenance of genomic stability. Among proteins involved in these processes, the ataxia-telangiectasia mutated (ATM) kinase is required for both activation of the DDR and the spindle assembly checkpoint (SAC). In mitosis without DNA damage, the enzymatic activity of ATM is enhanced; however, substrates of ATM in mitosis are unknown. Using stable isotope labeling of amino acids in cell culture mass spectrometry analysis, we identified a number of proteins that can potentially be phosphorylated by ATM during mitosis. This list is highly enriched in proteins involved in cell cycle regulation and the DDR. Among them, we further validated that ATM phosphorylated budding uninhibited by benzimidazoles 3 (Bub3), a major component of the SAC, on serine 135 (Ser135) both in vitro and in vivo. During mitosis, this phosphorylation promoted activation of another SAC component, benzimidazoles 1. Mutation of Bub3 Ser135 to alanine led to a defect in SAC activation. Furthermore, we found that ATM-mediated phosphorylation of Bub3 on Ser135 was also induced by ionizing radiation-induced DNA damage. However, this event resulted in independent signaling involving interaction with the Ku70-Ku80-DNA-PKcs sensor/kinase complex, leading to efficient nonhomologous end-joining repair. Taken together, we highlight the functional significance of the crosstalk between the kinetochore-oriented signal and double-strand break repair pathways via ATM phosphorylation of Bub3 on Ser135.

Investigation of seawater electrolyte on hydrogen evolution reaction from the perspective of kinetics and energy consumption using an Ni-based electrocatalyst supported on carbon nanotubes.

In this study, a Ni-based composite incorporating Ni4N and La2O3 supported on carbon nanotubes (Ni-La-Ni4N/CNT) was synthesized as an efficiency electrocatalyst towards the hydrogen evolution reaction in different electrolytes with the kinetics and energy consumption investigated in detail. The Ni-La-Ni4N/CNT exhibits overpotentials of 124 mV and 200 mV at the current density of 10 mA cm-2 in 1.0 M KOH and alkaline seawater, respectively. As quantitative comparison, the exchange current density (j°) based on Volmer-Heyrovsky-Tafel mechanism was calculated from various polarization curves, which indicated that the addition of NaCl in alkaline medium or using seawater alone reduced the reactivity of the catalyst. The activity of Ni-La-Ni4N/CNT in alkaline seawater was equal to 91% of that in 1.0 M KOH. Furthermore, dynamic polarization resistance and corresponding current were obtained by the analysis of the equivalent circuit model with the extended Kalman filter algorithm. The analysis of the resistance power at 1 mW also shows that the current between the conditions in KOH and in seawater is 2.76 times. Adding alkaline substances to seawater can narrow it to 1.19 times. These strategies provide novel approaches for inspecting the activity changes of materials in different electrochemical environments.

Electrochemical Biosensor Based on Horseradish Peroxidase and Black Phosphorene Quantum Dot Modified Electrode.

Black phosphorene quantum dots (BPQDs) were prepared by ultrasonic-assisted liquid-phase exfoliation and centrifugation with morphologies proved by TEM results. Furthermore, an electrochemical enzyme sensor was prepared by co-modification of BPQDs with horseradish peroxidase (HRP) on the surface of a carbon ionic liquid electrode (CILE) for the first time. The direct electrochemical behavior of HRP was studied with a pair of well-shaped voltammetric peaks that appeared, indicating that the existence of BPQDs was beneficial to accelerate the electron transfer rate between HRP and the electrode surface. This was due to the excellent properties of BPQDs, such as small particle size, high interfacial reaction activity, fast conductivity, and good biocompatibility. The presence of BPQDs on the electrode surface provided a fast channel for direct electron transfer of HRP. Therefore, the constructed electrochemical HRP biosensor was firstly used to investigate the electrocatalytic behavior of trichloroacetic acid (TCA) and potassium bromate (KBrO3), and the wide linear detection ranges of TCA and KBrO3 were 4.0-600.0 mmol/L and 2.0-57.0 mmol/L, respectively. The modified electrode was applied to the actual samples detection with satisfactory results.

Sutcliffiella deserti sp. nov., isolated from desert soil.

A Gram-stain-positive, facultative anaerobic, motile and rod-shaped bacterial strain, DG-18T, was isolated from desert soil sampled at the Kubuqi Desert in Inner Mongolia, China. Strain DG-18T grew at 4-40 °C (optimum, 25-30 °C), at pH 8.0-10.0 (optimum, pH 9.0) and with 0-8.0 % (w/v) NaCl (optimum 2.0%). 16S rRNA gene sequence analysis placed strain DG-18T within the genus Sutcliffiella of the family Bacillaceae with Sutcliffiella halmapala DSM 8723T (98.2%), Sutcliffiella zhanjiangensis JSM 099021T (97.6%), Sutcliffiella horikoshii DSM 8719T (97.4%), Sutcliffiella catenulata 18CT (96.6 %) and Sutcliffiella cohnii NBRC 15565T (96.5%) as its closest relatives. The major respiratory quinone of strain DG-18T was MK-7 and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Its major fatty acids were iso-C15 : 0 and iso-C17 : 1 ω10c. The genomic DNA G+C content of strain DG-18T was 38.7 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain DG-18T and that of S. halmapala DSM 8723T was 76.7 %. The Genome-to-Genome Distance Calculator showed that the DNA-DNA hybridization value for strain DG-18T and S. halmapala DSM 8723T was 21.8%. Based on the polyphasic taxonomic data, strain DG-18T represents a novel species of the genus Sutcliffiella, for which the name Sutcliffiella deserti sp. nov. is proposed. The type strain is DG-18T (=GDMCC 1.17773T=KCTC 43170T).

Does a beautiful environment make food better - The effect of environmental aesthetics on food perception and eating intention.

Generally, people prefer to dine in beautiful environments. Previous studies have reported that environmental factors affect an individual's perception of food; however, little is known about the effect of environmental aesthetics on food perception. In Experiment 1, we used photographs of restaurant (1a) or non-restaurant (1b) environments with high or low aesthetic value, paired with images of foods, and participants were asked to rate the visual, olfactory, and gustatory aesthetic value of the food. Results showed significantly higher ratings for food perception in all three sensory modalities in the high aesthetic value environment, together with positive emotion and the desire to eat, compared with the low aesthetic environment. Experiment 2 extended the study to two real-world environments (one high and one low aesthetic value) and actual food consumption. The results also found higher aesthetic ratings in the olfactory and gustatory systems and greater desire to eat again in an environment with high aesthetic value than in an environment with low aesthetic value. This research also explored the mediating role of emotion in the relationship between environmental aesthetics and food perception and found a significant mediating relationship. In conclusion, environmental aesthetics play an important role in food perception, and these findings provide insights into increasing positive food perception in daily life.

Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite.

To design a new electrochemical horseradish peroxidase (HRP) biosensor with excellent analytical performance, black phosphorene (BP) nanosheets and single-walled carbon nanotubes (SWCNTs) nanocomposites were used as the modifier, with a carbon ionic liquid electrode (CILE) as the substrate electrode. The SWCNTs-BP nanocomposite was synthesized by a simple in situ mixing procedure and modified on the CILE surface by the direct casting method. Then HRP was immobilized on the modified electrode with Nafion film. The electrocatalysis of this electrochemical HRP biosensor to various targets was further explored. Experimental results indicated that the direct electrochemistry of HRP was realized with a pair of symmetric and quasi-reversible redox peaks appeared, which was due to the presence of SWCNTs-BP on the surface of CILE, exhibiting synergistic effects with high electrical conductivity and good biocompatibility. Excellent electrocatalytic activity to trichloroacetic acid (TCA), sodium nitrite (NaNO2), and hydrogen peroxide (H2O2) were realized, with a wide linear range and a low detection limit. Different real samples, such as a medical facial peel solution, the soak water of pickled vegetables, and a 3% H2O2 disinfectant, were further analyzed, with satisfactory results, further proving the potential practical applications for the electrochemical biosensor.

Ugly food, not eat: the eating intention of emotional eater for different esthetic food.

BACKGROUND: Emotional eaters eat to relieve their emotions. However, food also contains esthetic information. People generally perceive ugly food as unhealthy and unpalatable. Does the esthetic information of food influence an emotional eater's desire for food in a negative emotional state? In particular, do they have the same lower eating intentions for low esthetic food as non-emotional eaters? OBJECTIVE/DESIGN/MEASURES: Based on these questions, the present study examined whether the esthetic value of food influences emotional eaters' desires for food. The experiment used a 2 (eating type: emotional eating vs. non-emotional eating) × 2 (food style: high esthetic vs. low esthetic) mixed experimental design. We measured the emotional and non-emotional eaters' eating intentions for different esthetic foods when experiencing negative emotions. RESULTS: The results showed that emotional eaters have higher intention to eat high esthetic foods. However, they did not have a high eating intention for all foods, and their eating intention did not differ from that of non-emotional eaters when faced with low esthetic food. CONCLUSION: In conclusion, food esthetic value can affect individual eating intentions. Even for emotional eaters who are in a negative mood, they also did not have a higher eating intention for low esthetic food compared with no-emotional eater. LEVEL OF EVIDENCE: Level II: controlled trial without randomization.

Planococcus soli sp. nov., isolated from antarctic soil.

A novel bacterial strain, designated C23T, was isolated from a soil sample obtained from King George Island, Antarctica. Phenotypic, phylogenetic, chemotaxonomic and molecular analyses were performed on the new isolate. Strain C23T formed orange colonies on agar plates and was Gram-stain-positive. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain C23T was a member of the genus Planococcus and was closely related to Planococcus salinarum DSM 23802T (98.5% sequence similarity), Planococcus halotolerans SCU63T (98.3%), Planomicrobium okeanokoites IFO 12526T (98.3%), Planococcus donghaensis DSM 22276T (98.3%) and Planococcus maitriensisi S1T (98.2%). This organism grew at 2-38 °C (optimum, 25-30 °C), pH 6.0-12.0 (optimum, pH 7.5) and 0-14% (w/v) NaCl (optimum, 2%). The predominant menaquinone was MK-8. The major cellular fatty acids were anteiso-C15:0, iso-C14:0 and C16:1 ω7c alcohol. The whole genome DNA of C23T was deposited in the GenBank database under accession number WXYN00000000. According to the whole genome, the DNA G + C content of strain C23T was determined to be 46.8 mol%; the average nucleotide identity (ANI) of strain C23T and P. salinarum DSM 23802T, P. halotolerans SCU63T, P. okeanokoites IFO 12526T, P. donghaensis DSM 22276T and P. maitriensis S1T were 74.1%, 74.3%, 74.1%, 78.8 and 73.6%; the digital DNA-DNA hybridization (dDDH) values between strain C23T and the five closely related species were 19.7%, 19.6%, 19.5%, 22.4 and 18.6%; the average amino acid identity (AAI) values between strain C23T and the five closely related species were 73.9%, 74.5%, 74.4%, 84.6 and 74.5%. All data were below the threshold range for species determination. Based on the polyphasic taxonomic study, we considered that strain C23T represented a novel species of the genus Planococcus for which the name Planococcus soli sp. nov. is proposed. The type strain is C23T (= KCTC 33644T = CGMCC 1.15115T).

Description and genome analysis of Microvirga antarctica sp. nov., a novel pink-pigmented psychrotolerant bacterium isolated from Antarctic soil.

A novel pink-pigmented bacterium, designated strain 3D7T, was isolated during an investigation of potential psychrotolerant species from Antarctic soil. Cells of the isolate were observed to be rod-shaped (0.7-0.9 × 1.0-2.2 µm), Gram-stain negative and non-motile. It was able to grow at 4-32  °C, pH 7.0-10.0 and in the presence of 0-3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 3D7T belongs to the genus Microvirga and was most closely related to 'Microvirga brassicacearum' CDVBN77T (98.3%), Microvirga subterranea DSM 14364 T (96.8%), Microvirga guangxiensis 25BT (96.5%) and Microvirga aerophila DSM 21344 T (96.5%). The predominant quinone was ubiquinone 10 (Q-10), and the major fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. The predominant polar lipids were phosphatidylcholine and phosphatidylethanolamine. The genomic DNA G + C content of strain 3D7T was 63.5 mol%. Its genome sequence showed genes encoding phosphatases and lipases. Genetic machinery related to carbohydrate-active enzymes and secondary metabolites were also observed. The average nucleotide identity and digital DNA-DNA hybridization values based on whole genome sequences of strain 3D7T and its closely related species were below the threshold range for species determination. Phenotypic, chemotaxonomic, phylogenetic and genomic analyses suggested that strain 3D7T represents a novel species of the genus Microvirga, for which the name Microvirga antarctica sp. nov. is proposed. The type strain is 3D7T (= CGMCC 1.13821T = KCTC 72465T).

Modeling the Capacitated Multi-Level Lot-Sizing Problem under Time-Varying Environments and a Fix-and-Optimize Solution Approach.

In this study, we investigated the time-varying capacitated lot-sizing problem under a fast-changing production environment, where production factors such as the setup costs, inventory-holding costs, production capacities, or even material prices may be subject to continuous changes during the entire planning horizon. Traditional lot-sizing theorems and algorithms, which often assume a constant production environment, are no longer fit for this situation. We analyzed the time-varying environment of today's agile enterprises and modeled the time-varying setup costs and the time-varying production capacities. Based on these, we presented two mixed-integer linear programming models for the time-varying capacitated single-level lot-sizing problem and the time-varying capacitated multi-level lot-sizing problem, respectively, with considerations on the impact of time-varying environments and dynamic capacity constraints. New properties of these models were analyzed on the solution's feasibility and optimality. The solution quality was evaluated in terms of the entropy which indicated that the optimized production system had a lower value than that of the unoptimized one. A number of computational experiments were conducted on well-known benchmark problem instances using the AMPL/CPLEX to verify the proposed models and to test the computational effectiveness and efficiency, which showed that the new models are applicable to the time-varying environment. Two of the benchmark problems were updated with new best-known solutions in the experiments.

Analysis of a complex produced in the Fenton oxidation process.

The absorbances of different concentrations of Fe(2+), Fe(3+) and H2O2 were investigated by UV-visible spectrophotometry without separating the substances. The law of complex formation was studied by considering changes in the UV-vis spectra of mixtures of these three substances. The results show that upon eliminating the influence of the substrate, an iron-based complex was present in the Fenton reaction, which exhibited substantial absorbance from 190 to 500 nm. Therefore, the presence of an unknown complex in the Fenton oxidation process was verified and its concentration varied with a change in the concentration of hydrogen peroxide. This study provides a strong foundation for further studies into the mechanism of traditional hydroxyl radical theory of the Fenton reaction.

The border tourism hotspots network based on travelogues.

This study applies the Apriori algorithm and social network analysis to analyze travelogue data from the Qinghai-Tibet Plateau, effectively mapping significant border tourism hotspots and their interconnections within defined networks. Our findings distinctly partition the region into two principal sub-networks: Xinjiang and Tibet, highlighting the geographical segmentation that potentially impedes comprehensive regional tourism integration. The analysis underscores a pronounced reliance on transportation, reflecting the inherently multi-destination nature of border tourism in the area. Key nodes within these networks include Kashgar City and Lhasa City, serving as central hubs in their respective sub-networks, while Gar County and Hotan County act as pivotal connectors bridging the two distinct areas. Additionally, Gyirong County, Nyalam County, and Tashkurgan Tajik Autonomous County are identified as well-developed border tourism destinations, with Gyirong County and Tashkurgan Tajik Autonomous County possessing substantial potential to evolve into core border tourism hubs. Drawing on these insights, the study proposes targeted development strategies to enhance the structure and efficacy of border tourism on the Qinghai-Tibet Plateau.

Diagnostic Efficacy of Type B Vessels in the Japan Esophageal Society Classification for the Depth of Invasion of Superficial Esophageal Squamous Cell Carcinoma.

AIM: The preoperative diagnostic method for superficial esophageal squamous cell carcinoma (SESCC) invasion depth based on the Japan Esophageal Society (JES) classification has been promoted. However, there have been a few investigations into its diagnostic performance in clinical settings. Therefore, we aimed to elucidate the actual diagnostic performance of the JES classification using a single-center retrospective study design. METHODS: We retrospectively analyzed the clinical data of 315 newly diagnosed SESCC patients who underwent narrow-band imaging magnifying endoscopy (NBI-ME) examination and received endoscopic submucosal dissection (ESD) or esophagectomy in our center during the past 5 years. To evaluate the diagnostic performance of JES classification in assessing the depth of invasion of SESCC, clinical data of these patients were collected, and the concordance between NBI-ME findings and postoperative pathology reports was analyzed. RESULTS: This study included a total of 338 lesions. The diagnostic accuracy of vascular morphology was 76.0%. The sensitivity (87.0%) and positive predictive value (PPV, 85.4%) of B1 vessels were high, but the specificity (42.0%) and negative predictive value (NPV, 45.3%) were low. The specificity (86.9% and 98.8%) and NPVs (87.5% and 96.8%) of B2 and B3 vessels were high, but the sensitivity (36.4% and 21.4%) and PPVs (35.1% and 42.9%) ware low. Furthermore, only a few lesions (n = 57) described avascular area, but the overall diagnostic accuracy was not ideal (21.1%). However, if lesions invading the superficial submucosa or shallower were included in the category of "suitable for ESD", the overall accuracy of the JES classification was found to be 95.6%. CONCLUSIONS: In actual clinical settings, the overall accuracy of the JES classification system decreases, but the diagnostic performance of each subtype retains its original characteristics. Additionally, this classification is appropriate for determining whether type 0-II SESCC lesions are suitable for ESD.

Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.).

The ALOG (Arabidopsis LSH1 and Oryza G1) family proteins, namely, DUF640 domain-containing proteins, have been reported to function as transcription factors in various plants. However, the understanding of the response and function of ALOG family genes during reproductive development and under abiotic stress is still largely limited. In this study, we comprehensively analyzed the structural characteristics of ALOG family proteins and their expression profiles during inflorescence development and under abiotic stress in rice. The results showed that OsG1/OsG1L1/2/3/4/5/6/7/8/9 all had four conserved helical structures and an inserted Zinc-Ribbon (ZnR), the other four proteins OsG1L10/11/12/13 lacked complete Helix-1 and Helix-2. In the ALOG gene promoters, there were abundant cis-acting elements, including ABA, MeJA, and drought-responsive elements. Most ALOG genes show a decrease in expression levels within 24 h under ABA and drought treatments, while OsG1L2 expression levels show an upregulated trend under ABA and drought treatments. The expression analysis at different stages of inflorescence development indicated that OsG1L1/2/3/8/11 were mainly expressed in the P1 stage; in the P4 stage, OsG1/OsG1L4/5/9/12 had a higher expression level. These results lay a good foundation for further studying the expression of rice ALOG family genes under abiotic stresses, and provide important experimental support for their functional research.

Corrigendum: Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.).

[This corrects the article DOI: 10.3389/fgene.2024.1381690.].

In Situ Polymerization Synthesis of Graphdiyne Nanosheets as Electrode Material and Its Application in NMR Spectroelectrochemistry.

In situ NMR spectroelectrochemistry is extremely powerful in studying redox reactions in real time and identifying unstable reaction intermediates. In this paper, in situ polymerization synthesis of ultrathin graphdiyne (GDY) nanosheets was realized on the surface of copper nanoflower/copper foam (nano-Cu/Cuf)-based electrode with hexakisbenzene monomers and pyridine. Palladium (Pd) nanoparticles were further deposited onto the GDY nanosheets by the constant potential method. By using this GDY composite as electrode material, a new NMR-electrochemical cell was designed for in situ NMR spectroelectrochemistry measurement. The three-electrode electrochemical system consists of a Pd/GDY/nano-Cu/Cuf electrode as the working electrode, a platinum wire as the counter electrode, and a silver/silver chloride (Ag/AgCl) wire as a quasi-reference electrode, which can be dipped into a specially constructed sample tube and adapted for convenient operation in any commercial high-field, variable-temperature FT NMR spectrometer. The application of this NMR-electrochemical cell is illustrated by monitoring the progressive oxidation of hydroquinone to benzoquinone by controlled-potential electrolysis in aqueous solution.