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Developing a low-protein feed is important for the sustainable advancement of aquaculture. The aim of this study was to investigate the effects of essential amino acid (EAA) supplementation in a low-protein diet on the growth, intestinal health, and microbiota of the juvenile blotched snakehead, Channa maculata in an 8-week trial conducted in a recirculating aquaculture system. Three isoenergetic diets were formulated to include a control group (48.66 % crude protein (CP), HP), a low protein group (42.54 % CP, LP), and a low protein supplementation EAA group (44.44 % CP, LP-AA). The results showed that significantly lower weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER), and feed efficiency ratio (FER) were observed in fish that were fed LP than in the HP and LP-AA groups (P < 0.05). The HP and LP-AA groups exhibited a significant increase in intestinal villus length, villus width, and muscular thickness compared to the LP group (P < 0.05). Additionally, the HP and LP-AA groups demonstrated significantly higher levels of intestinal total antioxidant capacity (T-AOC), catalase (CAT), and superoxide dismutase (SOD) and lower levels of malondialdehyde (MDA) compared to the LP group (P < 0.05). The apoptosis rate of intestinal cells in the LP group was significantly higher than those in the LP and HP groups (P < 0.05). The mRNA expression levels of superoxide dismutase (sod), nuclear factor kappa B p65 subunit (nfκb-p65), heat shock protein 70 (hsp70), and inhibitor of NF-κBα (iκba) in the intestine were significantly higher in the LP group than those in the HP and LP-AA groups (P < 0.05). The 16s RNA analysis indicated that EAA supplementation significantly increased the growth of Desulfovibrio and altered the intestinal microflora. The relative abundances of Firmicutes and Cyanobacteria were positively correlated with antioxidant parameters (CAT and T-AOC), whereas Desulfobacterota was negatively correlated with sod and T-AOC. The genera Bacillus, Bacteroides, and Rothia were associated with the favorable maintenance of gut health. In conclusion, dietary supplementation with EAAs to achieve a balanced amino acid profile could potentially reduce the dietary protein levels from 48.66 % to 44.44 % without adversely affecting the growth and intestinal health of juvenile blotched snakeheads.
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Aminoácidos Essenciais , Ração Animal , Suplementos Nutricionais , Microbioma Gastrointestinal , Intestinos , Animais , Ração Animal/análise , Suplementos Nutricionais/análise , Microbioma Gastrointestinal/efeitos dos fármacos , Aminoácidos Essenciais/administração & dosagem , Perciformes/crescimento & desenvolvimento , Perciformes/imunologia , Dieta com Restrição de Proteínas/veterinária , Dieta/veterinária , Distribuição Aleatória , Peixes/crescimento & desenvolvimento , Aquicultura , Channa punctatusRESUMO
Astrocyte-microglial interaction plays a crucial role in brain injury-associated neuroinflammation. Our previous data illustrated that astrocytes secrete microRNA, leading to anti-inflammatory effects on microglia. Long non-coding RNAs participate in neuroinflammation regulation after traumatic brain injury. However, the effect of astrocytes on microglial phenotype via long non-coding RNAs and the underlying molecular mechanisms remain elusive. We used long non-coding RNA sequencing on murine astrocytes and found that exosomal long non-coding RNA 4933431K23Rik attenuated traumatic brain injury-induced microglial activation in vitro and in vivo and ameliorated cognitive function deficiency. Furthermore, microRNA and messenger RNA sequencing together with binding prediction illustrated that exosomal long non-coding RNA 4933431K23Rik up-regulates E2F7 and TFAP2C expression by sponging miR-10a-5p. Additionally, E2F7 and TFAP2C, as transcription factors, regulated microglial Smad7 expression. Using Cx3cr1-Smad7 overexpression of adeno-associated virus, microglia specifically overexpressed Smad7 in the attenuation of neuroinflammation, resulting in less cognitive deficiency after traumatic brain injury. Mechanically, overexpressed Smad7 physically binds to IκBα and inhibits its ubiquitination, preventing NF-κB signaling activation. The Smad7 activator asiaticoside alleviates neuroinflammation and protects neuronal function in traumatic brain injury mice. This study revealed that an exosomal long non-coding RNA from astrocytes attenuates microglial activation after traumatic brain injury by up-regulating Smad7, providing a potential therapeutic target.
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Lesões Encefálicas Traumáticas , MicroRNAs , RNA Longo não Codificante , Camundongos , Animais , Microglia/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Astrócitos/metabolismo , Doenças Neuroinflamatórias , MicroRNAs/metabolismo , Lesões Encefálicas Traumáticas/genética , Lesões Encefálicas Traumáticas/metabolismo , Fenótipo , Camundongos Endogâmicos C57BLRESUMO
BACKGROUND: Water content and chlorophyll content are important indicators for monitoring rice growth status. Simultaneous detection of water content and chlorophyll content is of significance. Different varieties of rice show differences in phenotype, resulting in the difficulties of establishing a universal model. In this study, hyperspectral imaging was used to detect the Soil and Plant Analyzer Development (SPAD) values and water content of fresh rice leaves of three rice varieties (Jiahua 1, Xiushui 121 and Xiushui 134). RESULTS: Both partial least squares regression and convolutional neural networks were used to establish single-task and multi-task models. Transfer component analysis (TCA) was used as transfer learning to learn the common features to achieve an approximate identical distribution between any two varieties. Single-task and multi-task models were also built using the features of the source domain, and these models were applied to the target domain. These results indicated that for models of each rice variety the prediction accuracy of most multi-task models was close to that of single-task models. As for TCA, the results showed that the single-task model achieved good performance for all transfer learning tasks. CONCLUSION: Compared with the original model, good and differentiated results were obtained for the models using features learned by TCA for both the source domain and target domain. The multi-task models could be constructed to predict SPAD values and water content simultaneously and then transferred to another rice variety, which could improve the efficiency of model construction and realize rapid detection of rice growth indicators. © 2024 Society of Chemical Industry.
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N6-methyladenosine (m6A), an epigenetic modification on RNAs, plays an important role in many physiological and pathological processes. However, the involvement of m6A in goat uterus during early pregnancy remains largely unknown. In this study, we found that the total m6A level was increasing in goat uterus as early pregnancy progressed. Methyltransferase-like 3 (METTL3) is a core catalytic subunit of the m6A methyltransferase. We thus determined the expression and regulation of METTL3 in goat uterus. METTL3 was highly expressed in the luminal and glandular epithelia from day 16 (D16) to D25 of pregnancy, and it could be up-regulated by estrogen and progesterone in goat uterus and primary endometrial epithelial cells (EECs). In EECs, knockdown or overexpression of METTL3 resulted in a significant decrease or increase of cell proliferation, respectively. METTL3 knockdown reduced the m6A level of not only total RNA but also connective tissue growth factor (CTGF) mRNA. Luciferase assay suggested that METTL3 might target the potential m6A sites in the 3'untranslated region (3'UTR) of CTGF mRNA. Moreover, METTL3 positively regulated CTGF expression, and CTGF knockdown significantly counteracted the promoting effect of METTL3 overexpression on EEC proliferation. Collectively, METTL3 is dynamically expressed in goat uterus and can affect EEC proliferation by regulating CTGF in an m6A-dependent manner. Our results will lay a foundation for further studying the crucial mechanism of METTL3-mediated m6A modification in goat uterus during early pregnancy.
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Fator de Crescimento do Tecido Conjuntivo , Cabras , Animais , Feminino , Fator de Crescimento do Tecido Conjuntivo/genética , Cabras/genética , Metiltransferases/genética , Metiltransferases/metabolismo , Células Epiteliais/metabolismo , RNA Mensageiro/metabolismo , Proliferação de Células/genéticaRESUMO
Vacuolar H+-pumping pyrophosphatases (VPs) provide a proton gradient for Na+ sequestration in the tonoplast; however, the regulatory mechanisms of VPs in developing salt tolerance have not been fully elucidated. Here, we cloned a barley (Hordeum vulgare) VP gene (HVP10) that was identified previously as the HvNax3 gene. Homology analysis showed VP10 in plants had conserved structure and sequence and likely originated from the ancestors of the Ceramiales order of Rhodophyta (Cyanidioschyzon merolae). HVP10 was mainly expressed in roots and upregulated in response to salt stress. After salt treatment for 3 weeks, HVP10 knockdown (RNA interference) and knockout (CRISPR/Cas9 gene editing) barley plants showed greatly inhibited growth and higher shoot Na+ concentration, Na+ transportation rate and xylem Na+ loading relative to wild-type (WT) plants. Reverse transcription quantitative polymerase chain reaction and microelectronic Ion Flux Estimation results indicated that HVP10 likely modulates Na+ sequestration into the root vacuole by acting synergistically with Na+/H+ antiporters (HvNHX1 and HvNHX4) to enhance H+ efflux and K+ maintenance in roots. Moreover, transgenic rice (Oryza sativa) lines overexpressing HVP10 also showed higher salt tolerance than the WT at both seedling and adult stages with less Na+ translocation to shoots and higher grain yields under salt stress. This study reveals the molecular mechanism of HVP10 underlying salt tolerance and highlights its potential in improving crop salt tolerance.
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Hordeum/genética , Hordeum/metabolismo , Pirofosfatase Inorgânica/metabolismo , Raízes de Plantas/metabolismo , Tolerância ao Sal/genética , Tolerância ao Sal/fisiologia , Sódio/metabolismo , Evolução Biológica , Transporte Biológico/genética , Produtos Agrícolas/genética , Produtos Agrícolas/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genes de Plantas , Variação Genética , Genótipo , Raízes de Plantas/genética , Plantas Geneticamente Modificadas , Vacúolos/metabolismoRESUMO
Herein, a novel tough luminescent hydrogel with Europium is fabricated using a facile copolymerization process by introducing 2,2':6',2-terpyridine (TPy) into a dual physical cross-linked hydrogel. The obtained P(NAGA-co-MAAc)/Eu/TPy (x) (x refers to the feed ratio of NAGA to MAAc) hydrogels not only show outstanding mechanical performances (fracture strength, ≈2.5 MPa), but also give a special ability of rapid detection to low concentrations of zinc ions. Attractively, the theoretical limits of detection (LOD) of the hydrogel sensors are calculated as 1.6 µm, which is acceptable within the WHO limit. Furthermore, the continuous change in fluorescence of P(NAGA-co-MAAc)/Eu/TPy (10) strips upon contact with Zn2+ can be clearly observed by the naked eyes with the aid of a portable UV lamp, resulting in semi-quantitative naked-eyes detection through a standard colorimetric card. Moreover, by identifying the RGB value of the hydrogel sensor, it can also realize quantitative analysis. Therefore, excellence in sensing, simplicity in structure, and convenience in using make P(NAGA-co-MAAc)/Eu/TPy (10) hydrogel as a superior fluorescent chemosensor of Zn2+ ions.
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Hidrogéis , Luminescência , Hidrogéis/química , Íons/química , Fluorescência , Zinco/químicaRESUMO
The real-time monitoring of neurochemical release in vivo plays a critical role in understanding the biochemical process of the complex nervous system. Current technologies for such applications, including microdialysis and fast-scan cyclic voltammetry, suffer from limited spatiotemporal resolution or poor selectivity. Here, we report a soft implantable aptamer-graphene microtransistor probe for real-time monitoring of neurochemical release. As a demonstration, we show the monitoring of dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), and picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex vivo experiments, and in vivo studies in mice models highlight the key features and demonstrate the capability of capturing the dopamine release dynamics evoked by pharmacological stimulation, suggesting the potential applications in basic neuroscience studies and studying neurological disease-related processes. The developed system can be easily adapted for monitoring other neurochemicals and drugs by simply replacing the aptamers functionalized on the graphene microtransistors.
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Dopamina , Grafite , Animais , Camundongos , Norepinefrina , OligonucleotídeosRESUMO
Many fish species exhibit remarkable sexual dimorphism, with males possessing numerous advantageous traits for commercial production by aquaculture such as faster growth rate, more efficient food energy utilization for muscle development, and better breeding performance. Several studies have shown that a decrease in the number of primordial germ cells (PGCs) during early development leads predominantly to male progeny. In this study, we developed a method to obtain all-male zebrafish (Danio rerio) by targeted PGC ablation using the nitroreductase/metronidazole (NTR/Mtz) system. Embryos generated by female heterozygous Tg(nanos3:nfsB-mCherry-nanos3 3'UTR) and male wild-types (WTs) were treated with vehicle or Mtz. Compared to vehicle-treated controls, 5.0 and 10.0 mM Mtz treatment for 24 h significantly reduced the number of PGCs and yielded an exclusively male phenotype in adulthood. The gonads of offspring treated with 5.0 mM Mtz exhibited relatively normal morphology and histological characteristics. Furthermore, these males were able to chase females, spawn, and produce viable offspring, while about 20.0% of males treated with 10.0 mM Mtz were unable to produce viable offspring. The 5.0 mM Mtz treatment protocol may thus be suitable for large-scale production of fertile male offspring. Moreover, about half of these males were WT as evidenced by the absence of nfsB gene expression. It may thus be possible to breed an all-male WT fish population by Mtz-mediated PGC ablation.
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Perciformes , Peixe-Zebra , Animais , Masculino , Feminino , Peixe-Zebra/fisiologia , Proteínas de Peixe-Zebra/genética , Células Germinativas , Fertilidade , Perciformes/metabolismoRESUMO
A series of MnM/palygorskite (PG) (M = La, W, Mo, Sb, Mg) catalysts was prepared by the wetness co-impregnation method for low-temperature selective catalytic reduction (SCR) of NO with NH3. Conversion efficiency followed the order Sb > Mo > La > W > Mg. A combination of various physico-chemical techniques was used to investigate the influence of Sb-modified Mn/PG catalysts. MnSb0.156/PG catalyst showed highest NO conversion at low temperatures in the presence of SO2 which reveals that addition of Sb oxides effectively enhances the SCR activity of catalysts. A SO2 step-wise study showed that MnSb0.156/PG catalyst displays higher durable resistance to SO2 than Mn/PG catalyst, where the sulfating of active phase is greatly inhibited after Sb doping. Scanning electron microscopy and X-ray diffraction results showed that Sb loading enhances the dispersion of Mn oxides on the carrier surface. According to the results of characterization analyses, it is suggested that the main reason for the deactivation of Mn/PG is the formation of manganese sulfates which cause the permanent deactivation of Mn-based catalysts. For Sb-doped Mn/PG catalyst, SOx ad-species formed were mainly combined with SbOx rather than MnOx. This preferential interaction between SbOx and SO2 effectively shields the MnOx as active species from being sulfated by SO2 resulting in the improvement of SO2 tolerance on Sb-added catalyst. Multiple information support that, owing to the addition of Sb, original formed MnOx crystallite has been completely transformed into highly dispersed amorphous phase accounting for higher SCR activity.
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Amônia , Catálise , Compostos de Magnésio , Oxirredução , Compostos de Silício , TemperaturaRESUMO
Willows (Salix spp.) are characterized by having large biomass, high tolerance to flooding, and strong metal accumulation ability, exhibiting great promise in the phytoremediation of iron (Fe) from contaminated sites. In this study, the variation of Fe tolerance and accumulation in 24 willow clones was investigated with two levels of Fe(II)-EDTA, 0.025 mM (control) and 2.0 mM (treatment) by hydroponic system for 21 days in a greenhouse. Visual symptoms of Fe toxicity were observed in the leaves and roots of Fe sensitive clones. Clonal comparisons showed a great variation in Fe tolerance, and the high levels of Fe reduced biomass productions of most clones. Tolerance indexes (TIs) varied about five-fold based on shoot dry biomass and about six-fold based on root dry biomass among clones. Clones also exhibited a wide variation in Fe concentrations (mg g-1 DW), ranged from 0.80 to 3.41 in leaves, from 5.40 to 10.51 in stems, and from 3.25 to 17.10 in roots under Fe treatments among clones. Large differences varied in the transport of Fe from roots to aerial parts among clones. The results highlighted the selection of Salix clones with high resistance to Fe toxicity and high Fe accumulation to improve phytoremediation efficacy.
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Salix , Poluentes do Solo/análise , Biodegradação Ambiental , Hidroponia , Ferro , Folhas de Planta/química , Raízes de Plantas/químicaRESUMO
Willows (Salix spp.) have been regarded as one of interesting plants for phytofiltration of water contaminated with trace metals. In this paper, the clonal differences in tolerance and phytofiltration capacity of mixed metals (Mn, Zn, and Cu) were evaluated in greenhouse pilot-scale wetlands with a floating-support culture system. The results showed that broad clonal variations of biomass accumulation in response to mixed metals ranging from growth inhibition to stimulation. Clones differed in tolerance to multi-metals by tolerance index (TI) based on shoot and root biomass. We also found that wide variations in uptake and accumulation of three metals, which was related to species/clones and metal species. The willows showed high Mn and Zn translocation capacity from roots to leaves suitable for phytoextraction. In contrast, all clones had poor Cu translocation capacity, and Cu mainly retained in roots suitable for rhizofiltration. Among all Salix clones, clones SM30 and J903 had large phytofiltration potential for three metals with their high tolerance.
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Salix , Poluentes do Solo/análise , Biodegradação Ambiental , Biomassa , Cobre/análise , Manganês , Raízes de Plantas/química , Áreas Alagadas , Zinco/análiseRESUMO
Cadmium (Cd) accumulation and tolerance in seven ornamental willow genotypes were investigated at different Cd levels (0, 5, 25, 50, and 100 µM) through hydroponic culture in a greenhouse. Severe phytotoxic symptoms in leaves and roots including leaf chlorosis and root browning were noticed when grown at ≥ 50 µM Cd. All genotypes showed high tolerance to low Cd levels (5 µM and 25 µM Cd), in contrast to high Cd levels (i.e. 50 µM and 100 µM) that severely disturbed plant growth of all genotypes. Cadmium concentrations reached 165.0-1251.0 µg g-1 DW in leaves, 22.9-331.2 µg g-1 in stems among the genotypes at 5-25 µM Cd, and Cd mainly accumulated in the roots. Although willow tissues can accumulate relatively high Cd concentrations under high Cd levels (≥ 50 µM), they are not suitable for phytoextraction of Cd due to poor growth.
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Cádmio/análise , Salix/metabolismo , Poluentes do Solo/análise , Biodegradação Ambiental , Genótipo , Hidroponia , Folhas de Planta/química , Raízes de Plantas/químicaRESUMO
Rice grain chalkiness is a highly complex trait involved in multiple metabolic pathways and controlled by polygenes and growth conditions. To uncover novel aspects of chalkiness formation, we performed an integrated profiling of gene activity in the developing grains of a notched-belly rice mutant. Using exhaustive tandem mass spectrometry-based shotgun proteomics and whole-genome RNA sequencing to generate a nearly complete catalog of expressed mRNAs and proteins, we reliably identified 38,476 transcripts and 3,840 proteins. Comparison between the translucent part and chalky part of the notched-belly grains resulted in only a few differently express genes (240) and differently express proteins (363), thus making it possible to focus on 'core' genes or common pathways. Several novel key pathways were identified as of relevance to chalkiness formation, in particular the shift of C and N metabolism, the down-regulation of ribosomal proteins and the resulting low abundance of storage proteins especially the 13 kDa prolamin subunit, and the suppressed photosynthetic capacity in the pericarp of the chalky part. Further, genes and proteins as transporters for carbohydrates, amino acid/peptides, proteins, lipids and inorganic ions showed an increasing expression pattern in the chalky part of the notched-belly grains. Similarly, transcripts and proteins of receptors for auxin, ABA, ethylene and brassinosteroid were also up-regulated. In summary, this joint analysis of transcript and protein profiles provides a comprehensive reference map of gene activity regarding the physiological state in the chalky endosperm.
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Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas/fisiologia , Oryza/metabolismo , Proteoma/metabolismo , Sementes/metabolismo , Regulação para Baixo/genética , Grão Comestível/genética , Grão Comestível/metabolismo , Endosperma/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Mutação , Oryza/embriologia , Oryza/genética , Fenótipo , Fotossíntese , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Prolaminas/metabolismo , Proteômica/métodos , RNA Mensageiro/análise , Proteínas Ribossômicas , Sementes/crescimento & desenvolvimento , Análise de Sequência de RNA , Amido/metabolismo , Transcriptoma , Regulação para Cima/genéticaRESUMO
BACKGROUND: Grain chalkiness is a highly undesirable trait deleterious to rice appearance and milling quality. The physiological and molecular foundation of chalkiness formation is still partially understood, because of the complex interactions between multiple genes and growing environments. RESULTS: We report the untargeted metabolomic analysis of grains from a notched-belly mutant (DY1102) with high percentage of white-belly, which predominantly occurs in the bottom part proximal to the embryo. Metabolites in developing grains were profiled on the composite platforms of UPLC/MS/MS and GC/MS. Sampling times were 5, 10, 15, and 20 days after anthesis, the critical time points for chalkiness formation. A total of 214 metabolites were identified, covering most of the central metabolic pathways and partial secondary pathways including amino acids, carbohydrates, lipids, cofactors, peptides, nucleotides, phytohormones, and secondary metabolites. A comparison of the bottom chalky part and the upper translucent part of developing grains of DY1102 resulted in 180 metabolites related to chalkiness formation. CONCLUSIONS: Generally, in comparison to the translucent upper part, the chalky endosperm had lower levels of metabolites regarding carbon and nitrogen metabolism for synthesis of storage starch and protein, which was accompanied by perturbation of pathways participating in scavenging of reactive oxygen species, osmorugulation, cell wall synthesis, and mineral ion homeostasis. Based on these results, metabolic mechanism of chalkiness formation is discussed, with the role of embryo highlighted.
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Grão Comestível/genética , Regulação da Expressão Gênica de Plantas/genética , Oryza/genética , Oryza/metabolismo , Proteínas de Plantas/genética , Grão Comestível/anatomia & histologia , Grão Comestível/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Regulação da Expressão Gênica de Plantas/fisiologia , Metabolômica , Oryza/anatomia & histologia , Proteínas de Plantas/metabolismo , Espectrometria de Massas em TandemRESUMO
BACKGROUND: Chalkiness has a deleterious influence on rice appearance and milling quality. We identified a notched-belly mutant with a high percentage of white-belly, and thereby developed a novel comparison system that can minimize the influence of genetic background and growing conditions. Using this mutant, we examined the differences in chemical composition between chalky and translucent endosperm, with the aim of exploring relations between occurrence of chalkiness and accumulation of starch, protein and minerals. RESULTS: Comparisons showed a significant effect of chalkiness on chemical components in the endosperm. In general, occurrence of chalkiness resulted in higher total starch concentration and lower concentrations of the majority of the amino acids measured. Chalkiness also had a positive effect on the concentrations of As, Ba, Cd, Cr, Mn, Na, Sr and V, but was negatively correlated with those of B, Ca, Cu, Fe and Ni. By contrast, no significant chalkiness effect on P, phytic acid-P, K, Mg or Zn was observed. In addition, substantial influence of the embryo on endosperm composition was detected, with the embryo showing a negative effect on total protein, amino acids such as Arg, His, Leu, Lys, Phe and Tyr, and all the 17 minerals measured, excluding Ca, Cu, P and Sr. CONCLUSION: An inverse relation between starch and protein as well as amino acids was found with respect to chalkiness occurrence. Phytic acid and its colocalized elements K and Mg were not affected by chalkiness. The embryo exerted a marked influence on chemical components of the endosperm, in particular minerals, suggesting the necessity of examining the role of the embryo in chalkiness formation. © 2016 The Authors. Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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Aminoácidos/análise , Carboidratos da Dieta/análise , Mutação , Oryza/química , Proteínas de Vegetais Comestíveis/análise , Sementes/química , Oligoelementos/análise , Aminoácidos/metabolismo , China , Carboidratos da Dieta/metabolismo , Endosperma/química , Endosperma/genética , Endosperma/crescimento & desenvolvimento , Endosperma/metabolismo , Humanos , Magnésio/análise , Magnésio/metabolismo , Microscopia Eletrônica de Varredura , Valor Nutritivo , Oryza/genética , Oryza/crescimento & desenvolvimento , Oryza/metabolismo , Ácido Fítico/análise , Ácido Fítico/biossíntese , Proteínas de Vegetais Comestíveis/biossíntese , Potássio/análise , Potássio/metabolismo , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Amido/análise , Amido/biossíntese , Oligoelementos/metabolismoRESUMO
BACKGROUND: Grain chalkiness is a complex trait adversely affecting appearance and milling quality, and therefore has been one of principal targets for rice improvement. Eliminating chalkiness from rice has been a daunting task due to the complex interaction between genotype and environment and the lack of molecular markers. In addition, the molecular mechanisms underlying grain chalkiness formation are still imperfectly understood. RESULTS: We identified a notched-belly mutant (DY1102) with high percentage of white-belly, which only occurs in the bottom part proximal to the embryo. Using this mutant, a novel comparison system that can minimize the effect of genetic background and growing environment was developed. An iTRAQ-based comparative display of the proteins between the bottom chalky part and the upper translucent part of grains of DY1102 was performed. A total of 113 proteins responsible for chalkiness formation was identified. Among them, 70 proteins are up-regulated and 43 down-regulated. Approximately half of these differentially expressed proteins involved in central metabolic or regulatory pathways including carbohydrate metabolism (especially cell wall synthesis) and protein synthesis, folding and degradation, providing proteomic confirmation of the notion that chalkiness formation involves diverse but delicately regulated pathways. Protein metabolism was the most abundant category, accounting for 27.4% of the total differentially expressed proteins. In addition, down regulation of PDIL 2-3 and BiP was detected in the chalky tissue, indicating the important role of protein metabolism in grain chalkiness formation. CONCLUSIONS: Using this novel comparison system, our comprehensive survey of endosperm proteomics in the notched-belly mutant provides a valuable proteomic resource for the characterization of pathways contributing to chalkiness formation at molecular and biochemical levels.
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Marcação por Isótopo , Mutação/genética , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Proteômica/métodos , Sementes/metabolismo , Amilopectina/metabolismo , Regulação para Baixo/genética , Endosperma/metabolismo , Endosperma/ultraestrutura , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Oryza/embriologia , Oryza/genética , Oryza/ultraestrutura , Sementes/ultraestrutura , Amido/metabolismo , Regulação para Cima/genéticaRESUMO
Fourier ptychography (FP) is an enabling imaging technique that produces high-resolution complex-valued images with extended field coverages. However, when FP images a phase object with any specific spatial frequency, the captured images contain only constant values, rendering the recovery of the corresponding linear phase ramp impossible. This challenge is not unique to FP but also affects other common microscopy techniques -- a rather counterintuitive outcome given their widespread use in phase imaging. The underlying issue originates from the non-uniform phase transfer characteristic inherent in microscope systems, which impedes the conversion of object wavefields into discernible intensity variations. To address this challenge, we present spatially-coded Fourier ptychography (scFP), a new method that synergizes FP with spatial-domain coded detection for true quantitative phase imaging. In scFP, a flexible and detachable coded thin film is attached atop the image sensor in a regular FP setup. The spatial modulation of this thin film ensures a uniform phase response across the entire synthetic bandwidth. It improves reconstruction quality and corrects refractive index underestimation issues prevalent in conventional FP and related tomographic implementations. The inclusion of the coded thin film further adds a new dimension of measurement diversity in the spatial domain. The development of scFP is expected to catalyse new research directions and applications for phase imaging, emphasizing the need for true quantitative accuracy with uniform frequency response.
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Chronic cerebral ischemia (CCI) results in a prolonged insufficient blood supply to the brain tissue, leading to impaired neuronal function and subsequent impairment of cognitive and motor abilities. Our previous research showed that in mice with bilateral carotid artery stenosis, the collateral neovascularization post Encephalo-myo-synangiosis (EMS) treatment could be facilitated by bone marrow mesenchymal stem cells (MSCs) transplantation. Considering the advantages of biomaterials, we synthesized and modified a gelatin hydrogel for MSCs encapsulation. We then applied this hydrogel on the brain surface during EMS operation in rats with CCI, and evaluated its impact on cognitive performance and collateral circulation. Consequently, MSCs encapsulated in hydrogel significantly augment the therapeutic effects of EMS, potentially by promoting neovascularization, facilitating neuronal differentiation, and suppressing neuroinflammation. Furthermore, taking advantage of multi-RNA-sequencing and in silico analysis, we revealed that MSCs loaded in hydrogel regulate PDCD4 and CASP2 through the overexpression of miR-183-5p and miR-96-5p, thereby downregulating the expression of apoptosis-related proteins and inhibiting early apoptosis. In conclusion, a gelatin hydrogel to enhance the functionality of MSCs has been developed, and its combination with EMS treatment can improve the therapeutic effect in rats with CCI, suggesting its potential clinical benefit.
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Brain diseases present a significant obstacle to both global health and economic progress, owing to their elusive pathogenesis and the limited effectiveness of pharmaceutical interventions. Phototherapy has emerged as a promising non-invasive therapeutic modality for addressing age-related brain disorders, including stroke, Alzheimer's disease (AD), and Parkinson's disease (PD), among others. This review examines the recent progressions in phototherapeutic interventions. Firstly, the article elucidates the various wavelengths of visible light that possess the capability to penetrate the skin and skull, as well as the pathways of light stimulation, encompassing the eyes, skin, veins, and skull. Secondly, it deliberates on the molecular mechanisms of visible light on photosensitive proteins, within the context of brain disorders and other molecular pathways of light modulation. Lastly, the practical application of phototherapy in diverse clinical neurological disorders is indicated. Additionally, this review presents novel approaches that combine phototherapy and pharmacological interventions. Moreover, it outlines the limitations of phototherapeutics and proposes innovative strategies to improve the treatment of cerebral disorders.
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Doença de Alzheimer , Doença de Parkinson , Humanos , Fototerapia , Pele , Doença de Parkinson/patologia , Doença de Alzheimer/patologiaRESUMO
Coated fertilizers have been widely used to improve fertility in barren land. However, improving soil structure and water-retention capacity is also essential for arid and semi-arid areas with sandy soils to promote crop growth. Most currently available coated fertilizers rarely meet these requirements, limiting their application scope. Therefore, this study "tailored" pectin-montmorillonite (PM) multifunctional coatings for arid areas, featuring intercalation reactions and nanoscale entanglement between pectin and montmorillonite via hydrogen bonding and electrostatic and van der Waals forces. Notably, PM coatings have demonstrated an effective "relay" model of action. First, the PM-50 coating could act as a "shield" to protect urea pills, increasing the mechanical strength (82.12 %). Second, this coating prolonged the release longevity of urea (<0.5 h to 15 days). Further, the remaining coating performed a water-retention function. Subsequently, the degraded coating improved the soil properties. Thus, this coating facilitated the growth of wheat seedlings in a simulated arid environment. Moreover, the cytotoxicity test, life cycle assessment, and soil biodegradation experiment showed that the PM coating exhibited minimal environmental impact. Overall, the "relay" model of PM coating overcomes the application limitations of traditional coated fertilizers and provides a sustainable strategy for developing coating materials in soil degradation areas.