G-quadruplexes on chromosomal DNA negatively regulates topoisomerase 1 activity.

Human DNA topoisomerase 1 (Top1) is a crucial enzyme responsible for alleviating torsional stress on DNA during transcription and replication, thereby maintaining genome stability. Previous researches had found that non-working Top1 interacted extensively with chromosomal DNA in human cells. However, the reason for its retention on chromosomal DNA remained unclear. In this study, we discovered a close association between Top1 and chromosomal DNA, specifically linked to the presence of G-quadruplex (G4) structures. G4 structures, formed during transcription, trap Top1 and hinder its ability to relax neighboring DNAs. Disruption of the Top1-G4 interaction using G4 ligand relieved the inhibitory effect of G4 on Top1 activity, resulting in a further reduction of R-loop levels in cells. Additionally, the activation of Top1 through the use of a G4 ligand enhanced the toxicity of Top1 inhibitors towards cancer cells. Our study uncovers a negative regulation mechanism of human Top1 and highlights a novel pathway for activating Top1.

Multi-wavelength second-harmonic generation in a double-clad high nonlinear fiber induced by multiple phase-matching.

In this study, multi-wavelength second-harmonic generation (SHG) based on self-phase modulation (SPM) broadband supercontinuum (SC) was observed by employing a double-clad high nonlinear optical fiber (HNLF) in conjunction with a femtosecond laser. At a wavelength of 1050 nm and an average pump power of 320 mW, multiple phase-matching conditions were achieved, and SH signals of central wavelengths ∼530.7 nm, ∼525.1 nm, ∼503.5 nm, and ∼478.7 nm were observed, with SHG efficiency reaching ∼1.34 × 10-4. The SHG in this experiment can be attributed to the utilization of a doped optical fiber, where dopants create defect states, facilitating optical-chemical transformation and enhancing second-order polarization susceptibility. Additionally, theoretical simulations were conducted, aligning closely with the experimental findings. To the best of our knowledge, this work marks the first demonstration of multi-wavelength SHG in optical fibers. It offers a distinctive avenue for customizing multi-wavelength ultrafast light sources, exhibiting great application potential in the fields of medical diagnostics and optical sensing.

Er3+/Yb3+/Ho3+ tri-doped no-core fiber temperature sensor based on fluorescence intensity ratio technology: towards high stability and accurate measurements.

In this paper, the green upconversion (UC) fluorescence emission from E r 3+/Y b 3+/H o 3+ tri-doped tellurite glass is investigated for temperature sensing. The doping of H o 3+ ions not only enhances the chance of energy level transition but also avoids the influence of the thermal effect caused by the proximity of 2 H 11/2 and 4 S 3/2 energy levels. The luminescence characteristics at different Y b 3+ and H o 3+ ion concentration doping molar ratios were investigated, and the strongest luminescence characteristics were exhibited when the Y b 3+ ion concentration was at 5 mol% and H o 3+ at 0.2 mol%. Based on this, a tri-doped T e O 2-Z n O-B i 2 O 3 (TZB) no-core fiber was fabricated and connected with multimode fibers (MMFs) to form a temperature sensor. The temperature sensing performance of the tri-doped TZB temperature sensor was evaluated in detail over the temperature range of 255-365 K. The repeatability and stability of the temperature sensor was experimentally verified. The E r 3+/Y b 3+/H o 3+ tri-doped sensor can be used for noninvasive optical temperature sensing in the fields of environmental monitoring, biological sensing, and industrial process temperature control, etc.

Highly sensitive surface plasmon resonance temperature sensor based on a hollow core fiber multilayer structure.

A surface plasmon resonance (SPR) temperature sensor based on a hollow core fiber (HCF) is designed in this paper. The sensor is composed of a multi-mode fiber (MMF)-HCF-MMF structure, and the self-made HCF is deposited successively with a thin layer of Au film (50 nm in thickness), gold nanoparticles (10 nm in diameter) and polydimethylsiloxane (PDMS). A series of theoretical and experimental investiagtions are conducted, and the results are as follows: the proposed sensing structure only with Au film can effectively excite the SPR effect, with a sensitivity of (2200 ± 100) nm / RIU in the refractive index (RI) range of 1.3334-1.3811; after adding AuNPS, the sensitivity of the sensor is effectively improved, the sensitivity can be increased to (3100 ± 100) nm / RIU, and after the PDMS coating, temperature sensing can be realized due to its unique temperature-sensitive characteristics, a linear sensitivity of (-2.1 ± 0.1) nm / °C is realized in the temperature range of 25 °C to 100 °C. The sensor has the advantages of simple structure, wide application, large measurement range, high sensitivity, good stability and repeatability. Meanwhile, the internal air hole of HCF leaves a preparation channel for dual-parameter measurement. It has broad application prospect in medical treatment, environmental monitoring and manufacturing industry.

Transmissive fluorescent temperature sensor based on Er3+/Yb3+/Mo6+ tri-doped tellurite fiber for real-time thermal monitoring of motors using the FIR technique.

In this paper, we fabricate a transmissive fluorescent temperature sensor (TFTS) that based on Er3+/Yb3+/Mo6+ tri-doped tellurite fiber, which has the advantages of compactness and simplicity, corrosion resistance, high stability and anti-electromagnetic interference. The doping of Mo6+ ions will enhance the up-conversion (UC) fluorescence emission efficiency of Er3+ ions, thus improving the signal-to-noise ratio of TFTS. Using the fluorescence intensity ratio (FIR) technique, the real-time thermal monitoring performance of TFTS is evaluated experimentally. Apart from good stability, its maximum relative sensitivity is 0.01068 K-1 at 274 K in the measured temperature range. In addition, it is successfully used to monitor the temperature variation of the stator core and stator winding of the motor in actual operation. The results show that the maximum error between the FIR-demodulated temperature and the reference temperature is less than 1.2 K, which fully confirms the effectiveness of the TFTS for temperature monitoring. Finally, the FIR-based TFTS in this work is expected to provide a new solution for accurate and real-time thermal monitoring of motors and the like.

Two fiber-bundle-type FI/FO devices of low insertion loss and cross talk applied for connecting 13-core 5-mode fiber.

Two fiber-bundle-typed fan-in/fan-out (FI/FO) devices, "wavy hexagon-shaped silhouette" type (W-FI/FO) and "tortoise-shaped silhouette" type (T-FI/FO), have been proposed and manufactured based on tapering glass tubes for docking with a self-made 13-core 5-mode fiber. The W-FI/FO device consists of 19 5-mode fibers and has an extended layout based on the 13-core 5-mode fiber structure. It could dock multiple fibers with 19 or 13 cores of the same size standards. When connecting it with 13-core 5-mode, the average losses (ILs) of its five modes are 1.07 dB, 2.95 dB, 3.42 dB, 3.65 dB, and 4.38 dB. The cross talks of the five linearly polarized (LP) modes are -69.1 dB, -64.7 dB, -44.2 dB, -43.9 dB, and -39.1 dB. The T-FI/FO device has a similar core arrangement to the 13-core 5-mode fiber and its average ILs of the five LP modes are 0.23 dB, 1.31 dB, 2.09 dB, 2.66 dB, and 3.03 dB. The cross talks of its five LP modes between adjacent cores are -72.8 dB, -67.8 dB, -43.6 dB, -40.0 dB, and -35.3 dB. The IL and cross talk of the LP01 mode are of satisfactory values, which are 0.23 dB and -72.8 dB, respectively. These two proposed FI/FO devices are expected to be used for high-speed optical interconnection and fiber communication.

Multimode interferometric sensor based on the no-core tellurite optical fiber for cryogenic temperature detection.

In this paper, a no-core tellurite optical fiber (NCTOF)-based sensor was proposed for cryogenic temperature detection in refrigeration process. The ultraviolet adhesive (UVA) dual-curing method was operated to stablish a sandwich-like composite structure, in which a section of NCTOF was compactly sandwiched between two segments of silica fiber to form multimode interference. The temperature sensing characteristics in cryogenic range were experimentally investigated by monitoring the transmission spectral movement, where a high sensitivity of 105.6 pm/°C was achieved in the range of -20-0 °C and 51.6 pm/°C in the range of -20-25 °C. The excellent performance was consistent with the simulation analysis. The maximum repeatability standard deviation and stability wavelength error of the sensor are 0.9799 pm/°C and 0.1676 nm, respectively. To the best of our knowledge, this is the first report on using tellurite optical fibers for cryogenic temperature detection, and the UVA dual-curing method provides a reliable solution for the integration and practical application of tellurite optical fiber. The proposed sensor is simple in structure, easy in fabrication, low in cost and excellent in performance. It can be expected to be used in food refrigeration, air-conditioning engineering, medical and health, industrial production, etc.

Frequency conversions of nine peaks based on dispersive waves and solitons in a tellurite microstructured optical fiber.

We demonstrate the generation of broadband dispersive waves (DWs) and solitons in an 80-cm tellurite microstructured optical fiber (TMOF) designed and fabricated with 78TeO2-5ZnO-12LiCO3-5Bi2O3 (TZLB) glass. A 1810-nm femtosecond laser is used as the pump source with an average pump power ranging from 33 mW to 175 mW, where the tunable frequency range is 211.1 THz, which corresponds to the tunable wavelength range of 1742.9 nm. At 175 mW, the trapped multiple DWs are located at 923.8 nm, 1039.2 nm, 1121.6 nm, and 1204.6 nm and the multiple solitons are located at 2666.7 nm, 2426.1 nm, 2165.9 nm, 1952.7 nm, and 1842.1 nm. The experimentally obtained maximum DW conversion efficiency is 14%, and the maximum soliton conversion efficiency is 43%. The experimental and theoretical results of pulse evolution in the TMOF agree very well. To the best of our knowledge, this is the first time that nine peaks of frequency conversions have been realized simultaneously in non-silicon fibers. The exceptionally high nonlinearity and broadband-tunable characteristics of the proposed TMOF are promising components for the development of compact and highly efficient tunable mid-infrared fiber lasers, wavelength converters, and time-frequency metrology.

Single-mode tellurite optical fiber for temperature measurement based on the self-phase modulation effect.

In this paper, the self-phase modulation (SPM) effect in a double-cladding single-mode tellurite optical fiber (DC-SMTOF) was exploited for temperature sensing. The DC-SMTOF was fabricated based on a TeO2-ZnO-Li2O-Bi2O3 (TZLB) glass material that has a thermo-optical coefficient as high as -16.4×10-6/°C. The temperature sensing performance was evaluated by detecting the 3-dB bandwidth of the SPM spectra with the variation of temperature at different pump wavelength and different average pump power. The temperature sensitivity was obtained to be -2.971 nm/°C with a resolution of 0.0168°C. Both simulation and experiment confirmed that a longer pump wavelength and higher average pump power will result in a higher temperature sensitivity. To the best of our knowledge, this is the first study concerning SPM-based temperature sensing in a tellurite optical fiber. The proposed temperature sensor has a compact structure, and it can realize temperature sensing of high sensitivity without any fiber modification. This work opens the road toward explorations of a novel temperature sensing technology combined with soft glass fibers and nonlinear phenomenon, and is expected to deepen our understanding in the application of these complex nonlinear phenomena.

Plug-in tip sensor based on upconversion fluorescence in Er3+/Yb3+ co-doped tellurite glass for thermal monitoring of a miniature winding coil.

We demonstrate a plug-in tip sensor with a maximum cross section diameter of only 1 mm for real-time thermal monitoring of a high-density miniature winding coil, which can meet the miniaturization development needs of electromagnetic actuators. Due to the high upconversion luminescence efficiency, tellurite glass with an optimized Er3+/Yb3+ doping ratio is adhered to the end face of silica fiber for a temperature-sensitive tip. Temperature information is demodulated using the fluorescence intensity ratio technique, yielding a nonlinear response with R2 up to 0.9978. Within a wide temperature range of 253.55-442.45 K, the tip sensor exhibits good repeatability, excellent stability, high sensitivity of 52.7 × 10-4 K-1, small absolute error within ±1 K, and fast time response of 2.03 s. It has been successfully proven to be a miniaturized device with strong anti-interference ability for the health management of high-density winding coils.

Design of a free-form off-axis three-mirror optical system with a low f-number based on the same substrate.

In this paper, we design a free-form off-axis three-mirror optical system with a low f-number and compact structure, which can be used as an infrared reflection imager. The initial structure is calculated from the near-axis optical transfer matrix based on third-order aberration theory. Particular constraints are designed to install all mirrors on the same substrate for simultaneous milling, which reduces the processing difficulty and effectively avoids errors caused by component assembly. Zernike free-form surfaces are introduced to correct aberrations. This optical system has a field of view of 5∘×5∘ and an f-number of 1.82; the modulation transfer function of the system is higher than 0.6 at 30 lp/mm. The results of the tolerance assignment of the system were verified by the Monte Carlo method, and the machining tolerance is reasonable and easy to achieve. This design not only improves the optical performance of the system but also enhances the feasibility of manufacturing.

Cost-efficient and reliable annular optical fiber temperature sensor.

In this paper, an annular winding structure made of single-mode optical fiber is proposed as the core of a cost-efficient and reliable annular optical fiber temperature sensor (AOFTS). The sensor is mainly due to the multi-mode interference effect to achieve real-time response to external temperature changes. The experimental results show that the average temperature sensitivity of the sensor is about 255.5 pm/°C in the temperature range of -20∘C-110∘C; it has higher sensitivity at low temperatures. At -20∘C, the sensitivity of the AOFTS reaches 450 pm/°C. The sensor has the advantages of simple fabrication, low fabrication cost, strong stability, and good reproducibility and repeatability. It has great application prospects in the field of low-temperature detection.

Experimental investigation of supercontinuum generation in a birefringence tellurite microstructured optical fiber.

A four-hole birefringence tellurite microstructured optical fiber (BTMOF) was designed and fabricated based on 76.5T e O 2-6Z n O-11.5L i 2 O-6B i 2 O 3 glass, and its core (slow and fast axes were) measured to be approximately 4.74 µm and 4.29 µm, respectively. The experimentally measured results demonstrated that the maximum supercontinuum (SC) spectra extended from ∼914.1n m to ∼1885.1n m when the polarization state of the pump pulse was parallel to the fast axis at 1400 nm with an average power of 460 mW. We performed numerical simulations based on the nonlinear Schrödinger equation, which support the experimentally measured results. The SC generation in birefringent silica microstructured fiber with the same geometric parameters was simulated, and the results showed that the enhanced nonlinear refractive index of the BTMOF yielded a spectrum with a significantly larger bandwidth. Furthermore, the two polarization states along the fast axis and slow axis exhibit different dispersion characteristics, which provide a convenient way of tuning the properties of the generated SC. This work highlights BTMOF as a promising platform for the development of a SC light source, which can be widely used in food quality inspection, early cancer diagnostics, gas sensing, and high-spatial-resolution imaging.

Physiological Adaptation Mechanisms to Drought and Rewatering in Water-Saving and Drought-Resistant Rice.

Water-saving and drought-resistant rice (WDR) has high a yield potential in drought. However, the photosynthetic adaptation mechanisms of WDR to drought and rehydration have yet to be conclusively determined. Hanyou 73 (HY73, WDR) and Huanghuazhan (HHZ, drought-sensitive cultivar) rice cultivars were subjected to drought stress and rewatering when the soil water potential was −180 KPa in the booting stage. The leaf physiological characteristics were dynamically determined at 0 KPa, −30 KPa, −70 KPa, −180 KPa, the first, the fifth, and the tenth day after rewatering. It was found that the maximum net photosynthetic rate (Amax) and light saturation point were decreased under drought conditions in both cultivars. The change in dark respiration rate (Rd) in HY73 was not significant, but was markedly different in HHZ. After rewatering, the photosynthetic parameters of HY73 completely returned to the initial state, while the indices in HHZ did not recover. The antioxidant enzyme activities and osmoregulatory substance levels increased with worsening drought conditions and decreased with rewatering duration. HY73 had higher peroxidase (POD) activity as well as proline levels, and lower catalase (CAT) activity, ascorbate peroxidase (APX) activity, malondialdehyde (MDA) level, and soluble protein (SP) content during all of the assessment periods compared with HHZ. In addition, Amax was markedly negatively correlated with superoxide dismutase (SOD), POD, CAT, and SP in HY73 (p < 0.001), while in HHZ, it was negatively correlated with SOD, CAT, APX, MDA, Pro, and SP, and positively correlated with Rd (p < 0.001). These results suggest that WDR has a more simplified adaptation mechanism to protect photosynthetic apparatus from damage in drought and rehydration compared with drought-sensitive cultivars. The high POD activity and great SP content would be considered as important physiological bases to maintain high photosynthetic production potential in WDR.

No-core optical fiber sensor based on surface plasmon resonance for glucose solution concentration and temperature measurement.

The accuracy of the surface plasmon resonance (SPR) optical fiber sensor is affected by the change of ambient temperature. Therefore, we propose a simple dual channel SPR optical fiber sensor, which can measure both glucose concentration and ambient temperature. The proposed sensor is a two-channel structure based on a no-core optical fiber (NCF): one channel is coated with gold film and polydimethylsiloxane (PDMS) to sense the ambient temperature, and the other is coated with silver film to sense glucose concentration. The experimental results show that the sensor's sensitivity for sensing glucose concentration is 2.882 nm / %, and for sensing temperature is -2.904 nm / °C. By monitoring the real-time temperature, the accuracy of glucose concentration detection was improved. The proposed sensor has a simple and compact structure, and it is suitable for sensing glucose solution or other analyte solutions that need temperature compensation.

Microstructured optical fiber temperature sensor based on the self-phase modulation effect.

In this paper, we proposed a highly sensitive temperature sensor based on self-phase modulation (SPM) in an in-house fabricated microstructured optical fiber (MOF) which had three rings of air holes. The temperature sensing performance was evaluated by detecting the 3 dB bandwidth of SPM spectrum with the variation of temperature at different pump wavelengths and average pump power. At the pump wavelength of 1400 nm with the average pump power of 600 mW, the temperature sensitivity was obtained to be as high as 1.296 nm/°C. Moreover, the theoretical simulation was carried out, the results of which corresponded well with the experiment. To the best of our knowledge, this is the first experimental study concerning SPM-based temperature sensing. This work proves experimentally and theoretically a new temperature sensing mechanism drawing on the SPM effect in optical fibers, which is expected to develop temperature sensors of low cost, simple structure and high sensitivity.

Passively mode-locked thulium-doped fiber laser based on saturable absorption of carbon nanofibers.

As a new type of carbon-based material, carbon nanofibers (CNFs) have attracted much attention due to their unique physical structure and optical properties. In this paper, we propose the application of CNFs as the saturable absorber (SA) and established a passively mode-locked thulium-doped fiber laser (TDFL) for verification. By mixing sodium carboxymethyl cellulose solution with CNFs, CNF SA was prepared, the nonlinearity of which was tested as follows: the modulation depth was ∼1.3%, and the saturation intensity was 18MW/cm2. By inserting the CNF SA into the TDFL ring cavity, mode-locked laser pulses of a central wavelength of 1954.47 nm and a 3 dB bandwidth of 5.93 nm were obtained. The spectral pulse width was 1.31 ps; the repetition frequency was 32.68 MHz; and the signal-to-noise ratio (SNR) was calculated to be ∼57dB. To our knowledge, this is the first time that CNFs have been reported as SAs for mode-locked lasers in the 2 µm wavelength region. Our work provides a new reference for using carbon-based materials in the realization of ultrafast lasers, and the proposed CNFs are highly advantageous in the development of ultrahigh-speed optical modulators and next-generation high-performance nonlinear photonic devices.

Mid-infrared cascaded stimulated Raman scattering and flat supercontinuum generation in an As-S optical fiber pump at 2 µm.

We demonstrate broadband mid-infrared cascaded stimulated Raman scattering (SRS) and flat supercontinuum (SC) generation in a chalcogenide optical fiber made from As2S5 glass. By using a 2 µm nanosecond laser as the pump source, mid-infrared cascaded SRS up to six orders ranging from 2149 to 3425 nm was experimentally observed, and this all-fiber Raman laser operating at 3.43 µm was realized for the first time to our knowledge. By introducing a 2 µm femtosecond laser as the excited source, the broadband flat mid-infrared SC with the spectral range of ∼10dB (from ∼1030 to 3441 nm) was observed. Our results verify that the As2S5 optical fibers possess promising applications for tunable mid-infrared Raman fiber lasers and SC light sources pumped by 2 µm pulsed lasers.

Free-form off-axis three-mirror optical antenna design with an integrated primary/tertiary-mirror structure.

In order to improve the performance and simplify the structure of an optical antenna for a space laser communication system, we design a free-form off-axis three-mirror optical antenna with an integrated primary/tertiary-mirror structure. The adoption of the integrated primary/tertiary-mirror structure improves efficiency of light energy utilization and reduces the complexity of optical processing and assembly. The introduction of free-form optical elements and optical structure constraints helps to correct the off-axis aberration and realize a large field of view. The obtained optical antenna has the magnification of five times and field of view of 2.4∘×2.4∘. The image quality obtained here reaches the diffraction-limited level. At the communication wavelength of 808 nm, the wavefront error is better than λ/22, and the system has a high energy concentration. The proposed optical antenna could not only improve tracking accuracy of the optical antenna in space but also greatly reduce the complexity of the laser communication system. It has reference significance and application value for free-space laser communication.

Supercontinuum-induced multi-wavelength third-harmonic generation in a suspended-core microstructured optical fiber.

In this paper, we reported a multi-wavelength third-harmonic generation (THG) induced by supercontinuum (SC) in an in-house fabricated suspended-core microstructured optical fiber (MOF). The adjustment of pump wavelength and pump power exerted an influence on SC which simultaneously emitted third harmonic (TH) waves in the visible light range. At the pump wavelength of 1220 nm and the average pump power of 450 mW, a multi-wavelength TH spectrum (373∼589 nm) with over twenty distinct peaks was observed under the phase matching (PM) condition between the fundamental mode and the higher-order modes. To the best of our knowledge, this is the first report on THG in optical fibers with so great a number of wavelengths. The maximal THG conversion efficiency ∼6.791 × 10-4 was obtained at 1480 nm, 350 mW, which is highly competitive compared with the values reported previously. Furthermore, theoretical simulation has been carried out, which corresponded well with the experimental observation. This multi-wavelength THG in the suspended-core MOF may provide a unique pathway towards tailored multi-wavelength ultrafast light sources for applications in sensing and imaging technologies.