[Research on coronavirus disease 2019 field disinfection technology for prevention and control].

The coronavirus disease 2019 (COVID-19) epidemic has had a serious impact in the world. In the absence of vaccines and therapeutic drugs, disinfection has become an important technical means to block the spread of the virus. By analyzing the characteristics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), we studied a series of disinfection technologies for COVID-19. During the outbreak of COVID-19, Jinan Second Center for Disease Control and Prevention disinfected the houses of the cases to be investigated in a community. The effectiveness of the disinfection technology was verified through the process of disinfection preparation, sampling before disinfection, field disinfection, sampling after disinfection and evaluation of disinfection effect. Compared the data before and after disinfection, the killing rate of the total bacterial colonies in the air and on the surface of the object was more than or equal to 90%, and no SARS-CoV-2 was detected after disinfection. The results show that the disinfection effect of the disinfection technology meets the standard. Finally, through the analysis of the wrong way of disinfection and the harm of over disinfection, the importance of scientific disinfection and precise disinfection are emphasized, and the research has a good guiding value for prevention and control of the epidemic.

Research on Subdivision System of Sin-Cos Encoder Based on Zero Phase Bandpass Filter.

A novel high-precision subdivision system for high-speed encoders is designed in this work. The system is designed with an arc second of Sin-Cos Encoder (SCE) based on zero phase bandpass filter. The system collects the analog output signals of an encoder with a high-speed data acquisition system (DAS); the noise of a digital signal can be effectively eliminated by zero phase bandpass filter with appropriate prior parameters. Finally, the actual rotation angle of the encoder is calculated by the software subdivision technique in the system. The software subdivision technique includes two methods, which are the Analog Pulse Counter (APC) and the Arc Tangent Subdivision (ATS). The APC method calculates the encoder angle by counting the analog pulses acquired by the arc tangent signal. The ATS method calculates the encoder angle by computing the arc tangent results of each point. The accuracy and stability of the system are first verified with a simulated signal; second, the real signals of an SCE are acquired by a high speed DAS on a test bench of a precision reducer, which is employed in industrial robots. The results of the proposed system are compared. The experimental results show that the system can significantly improve the accuracy of the encoder angle calculation, with controllable costs.

Novel circular RNA 2960 contributes to secondary damage of spinal cord injury by sponging miRNA-124.

Recent studies have shown that circular RNAs (circRNAs) are involved in many human diseases, but their roles in secondary damage after spinal cord injury (SCI) remain unclear. In the current study circRNA sequencing was performed in the damaged tissues of SCI rats on the seventh day after injury, and related molecular mechanisms were investigated. Quantitative PCR validations of molecules that exhibited significantly altered expression in SCI mice were performed. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to assess differentially expressed circRNAs. A novel circRNA-2960 was the most significantly upregulated in the SCI group. It could downregulate its target molecule miRNA-124, then exacerbate the inflammatory response and induce apoptosis at the lesion site. Disrupting circRNA-2960 expression promoted recovery of tissues affected by secondary SCI damage. The results of the present study may provide new insight into the mechanisms of secondary injury in SCI, and a new molecular marker for the diagnosis and treatment of SCI.

Treatment of spinal tuberculosis with ultrashort-course chemotherapy in conjunction with partial excision of pathologic vertebrae.

BACKGROUND CONTEXT: The ultrashort-course chemotherapeutical scheme of less than 6 months has been used for part of patients with pulmonary tuberculosis and satisfactory curative effects have already been achieved. However, few systematic and clinical reports so far about medical treatment of spinal tuberculosis by using ultrashort-course chemotherapeutical schemes have been published in the spine-care literature. PURPOSE: To assess the results of ultrashort-course chemotherapy (UCC) in conjunction with partial excision of pathological vertebrae for spinal tuberculosis. STUDY DESIGN/SETTING: This is a retrospective comparative study of case series from a single center. PATIENT SAMPLE: Seventy-six cases of spinal tuberculosis, treated during 1998 and 2003 by senior author, were reviewed. All the cases underwent chemotherapies in conjunction with the uniform partial excision of pathological vertebra and had a minimum follow-up of 2 years. OUTCOME MEASURES: Clinical manifestations, laboratory tests, imaging examination, examination by ultrasonic wave B, drug complications, and clinical effects based on the previously described evaluative measures. METHODS: Of the 76 cases, 28 had UCC with the scheme of 2SHRZ/2.5H(2)R(2)Z(2), 23 had short-course chemotherapy (SCC) with the scheme of 3SHRZ/5H(2)R(2)Z(2), and 25 had standard chemotherapy (SC) with the scheme of 3SHRZ/9H(2)R(2)Z(2). All the patients had anterior partial excisions of pathological vertebrae, large iliac strut graft, and anterior or posterior fixation. The mean time of follow-up surveys for the ultrashort-course, short-course, and standard chemotherapy cases was 42.3 m, 46.5 m, and 55.4 m, respectively. RESULTS: The observance indices included 1) clinical manifestations: disappearance of tuberculosis symptoms, no CC pains, recovery of normal life or work, no percussion pains on pathologic sites, and recovery of neural functions; 2) laboratory tests: normal or close to normal test results of both erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) or one of them; 3) imaging examinations: X-ray films, computed tomography scan, and magnetic resonance imaging examinations show disappearance of abscesses, no new destructive foci, bone union on the bone grafting interface, satisfactory correction of deformities, and less than 5 degrees of the angle loss of deformity corrections; 4) examination by ultrasonic wave B: no opaque dark area of fluid sonolucent areas identified in possible sites of paravertebral abscesses or gravitation abscesses; and 5) drug complications: hepatic and renal functions, nervus vestibularis lesion, and gastrointestinal tract reactions. All the cases met the protetrakis indices and obtained complete clinical cure of spinal tuberculosis in the last follow-up. The significant differences of major drug complications were found among the 3 groups, with 5 cases of UCC (18%), 15 cases of SCC (65%), and 19 cases of SC (76%). The lasting chemotherapeutic lesion of liver, kidney, or the permanent nervus vestibularis lesion were found 3 cases in SCC, 5 cases in SC, and no case in UCC group. CONCLUSIONS: No significant differences in clinical cure rate were found among 3 groups. UCC in conjunction with anterior partial excisions of pathological vertebrae, large iliac strut graft, and anterior or posterior internal instrumental fixation achieved excellent clinical results and the lowest complication rate of antituberculosis chemotherapy.

Treatment for early postoperative esophageal fistula complicated with anterior cervical surgery.

OBJECT: To investigate the efficacy of debridement, open drainage, and early feeding to treat early esophageal fistula complicated with anterior cervical surgery. METHODS: Retrospective analysis was conducted on data from 3154 patients who had undergone anterior cervical surgeries for cervical vertebra diseases between January 2006 and January 2013, in which eight patients had esophageal fistula with five males and three females. Four patients had cervical spinal injuries and four patients had cervical spondylosis. All of whom had postoperative esophageal fistulas and underwent debridement and drainage. The wounds were left open for natural drainage. No drainage devices were placed in surgical sites, and no gastric tubes were placed after surgeries. Such normal food as rice balls but not liquid or semiliquid diet was recommended. Local pressurization was conducted with fists during feeding to prevent food overflowing from orificium fistulae. RESULTS: Healed esophageal fistulas were achieved in all of the patients after 1-2 weeks treatment. There weren't recurrent esophageal fistulas and delayed infection found during 2-5 years follow-up. Good swallowing functions and stable cervical vertebrae were achieved in all of the patients. The satisfactory therapeutic effects were achieved in patients with previous neck diseases. Frankel classifications were increased by 1-2 grades in patients with cervical spinal injuries. JOA scores were increased from 9.5 before surgery to 15.5 after surgery in patients with cervical vertebra diseases. CONCLUSIONS: Early postoperative esophageal fistula complication after anterior cervical surgery can successfully be treated by debridement, drainage without gastric tube, and with early oral postoperative feeding but without fluids.

[The clinical causes of the thoracic ossification of ligamentum flavum].

OBJECTIVE: To assess the different causes of thoracic ossification of the ligamentum flavum (TOLF). METHODS: From July 1989 to November 2005, 142 cases were diagnosed the TOLF, in which 121 were operated. The lesions were classified into three types on the basis of the clinical result: (1) In such primary group (Group 1, 90 cases), without incorporation disease and Ca, P and AKP was all normal; (2) In systemic ossified TOLF group (Group 2, 30 cases), 6 cases ankylosing spondylitis, 3 cases DISH, 10 cases fluorosis, 11 cases OPLL; (3) In local spine disease group (Group 3, 22 cases), 5 cases fracture in spine, 4 cases spine TB, 13 cases posterior marginal intraosseous cartilaginous node. Such clinical feature was analysed, moreover surveyed the thoracic kyphosis angle, upper thoracic kyphosis angle, lower thoracic kyphosis angle and the vertebra body wedge change. The effect was assessed using Epstein Scale. RESULTS: (1) In Group 1, the mainly type was connected type (67/90, 74%). The ossified ligamentum flavum was mainly located at the lower thoracic and thoracic-lumber levels. The local type was less. In Group 2, the mainly type was connected type (21/30, 70%). The local type was none. The lesions figure was the most. In Group 3, the local type was the most (18/22, 82%). (2) In Group 1, the ossified ligamentum flavum was mainly located at the upper and lower thoracic levels (225/486, 47%). In Group 2, mainly located at the whole thoracic, some include cervix and lumber. In Group 3, mainly location was related with the location of primary disease. (3) In group 1, the curve was normal in 81% (73/90) of cases. In Group 2, the curve was abnormal in 87% (26/30) of cases. In Group 3, the curve was normal in the 82% (18/22) of cases. CONCLUSIONS: The TOLF relates with systemic ossify disease, the change of load on the spine, aging and so on. It should be classified according to its causes.

[The research of bone morphogenetic protein expression, CT value and mature degree of ossification in the thoracic ossification of ligamentum flavum].

OBJECTIVE: To investigate the correlation of pathology, bone morphogentic protein (BMP) expression, CT value with the ossification of thoracic ligamentum flavum (TOLF) to afford the evidence to choose appropriate treatment methods. METHODS: Twenty-three patients aged 35 - 65 years old had TOLF in my hospital as case. Their courses of disease were 2 months to 9 years. The values of blood calcium, blood phosphorus and AKP in them were normal. The 5 peoples aged 21 - 35 years old who presented fracture of thoracic but not the ligamentum flavum ossification were selected as control. We excluded those who have DISH, ankylosing spondylitis, fluorosis and other disease related with TOLF. The lesion locus were scanned and mensurated by CT. The pathology characteristics were classified into immature ossification and mature ossification by general observation, histology examination. BMP were measured by the immunohistochemical (IHC) staining techniques. RESULTS: The CT value was significantly higher in the case group (547.2 +/- 131.4) than controlled group (137.7 +/- 10.6) (t = 6.922, P = 0.000). Further, the CT value in the mature ossification (702.9 +/- 17.7) was significantly higher than the immature (480.5 +/- 180.2) (t = 5.623, P = 0.000). In addition, BMP both expressed negative in the mature ossification and the controlled group, but positive in the immature ossification. BMP expression was significantly different between the immature ossification and the mature (chi2 = 70.000, P = 0.000). CONCLUSIONS: The CT values, pathological types and BMP expression results are similar to evaluate the ossification degrees of ligamentum flavum, and then could be indirectly judged the maturation degrees of TOLF by CT to confirm the treatment methods before operation.

[Emission characteristics of PM2.5 from blast furnace iron making].

Electrical low pressure impactor (ELPI) was used to online analyze the PM2.5 particle size and mass concentration distribution in the trapping field and ore tank of blast furnace iron-making plant. Results showed that the grain number concentration of PM2.5 in trapping field after dust removal was in the range of 10(5)-10(6)cm-3 , and the particle size was mainly below 0. 1 µm. While the grain number concentration of the PM2.5 in ore tank after dust removal was in the range of 10(4)-10(5) cm-3, the particle size was mainly below 1.0 µm, and the mass concentration distribution showed a single peak. The micro-morphology of PM2.5 monomer was mainly divided into two categories, spherical particles and irregular aggregates. Chemical composition analysis indicated that the concentrations of water soluble SO(2-)(4) , K+ , Ca2+ were higher than other ions in PM2.5, with the percentage of 10. 32% -28.55% , 10. 36% -12. 15% , 3.97% -15. 4% , respectively. The major elements was Fe, Si, Al, with 16. 8% -31. 62% , 2. 24% -8.76% , 1.24% -5. 89% of total mass, respectively; organic carbon and elementary carbon were 2. 7% -4. 6% and 0. 8% -1. 3% , respectively. The emission factors of PM2.5 in trapping field and in ore tank after dust removal were ranged from 0.045 to 0.085 kg t(-1) and 0.042 to 0.071 kg t-1, respectively.

[Comparative study of different operating methods in treating old thoracolumbar fractures with spinal cord injury].

OBJECTIVE: To compare the surgical efficacy of different operating methods for treating old thoracolumbar fracture with spinal cord injury. METHODS: From September 2000 to March 2006, 34 cases of old thoracolumbar fractures with spinal cord injury were treated. Patients were divided into 2 groups randomly. Group A (n = 18): anterior approach osteotomy, iliac bone graft and internal fixation were used. There were 10 males and 8 females with the age of 17-54 years. The apex level of kyphosis was T11 in 2 cases, T12 in 5 cases, L1 in 8 cases and L2 in 3 cases. The average preoperative Cobb angle of kyphosis was (36.33 +/- 3.13) degrees, and the average preoperative difference in height between anterior and posterior of involved vertebra was (22.34 +/- 11.61) mm. Neurological dysfunction JOA score was 10.44 +/- 1.12. Group B (n = 16): transpedicular posterior decompression and internal fixation were used. There were 8 males and 8 females with the age of 18-56 years. The apex level of kyphosis was T11 in 2 cases, T12 in 6 cases, L1 in 7 cases and L2 in 1 case. The preoperative Cobb angle of kyphosis was (38.55 +/- 4.22) degrees, and the preoperative difference in height between anterior and posterior of involved vertebra was (20.61 +/- 10.22) mm. Neurological dysfunction JOA score was 10.23 +/- 2.23. RESULTS: All the patients were followed up for 9-46 months with an average of 13.5 months. Cobb angle was (12.78 +/- 3.76) degrees in group A, which was improved by (24.23 +/- 1.64) degrees compared to that of preoperation; and was (10.56 +/- 4.23)degrees in group B, which was improved by (26.66 +/- 1.66) degrees. JOA score was 14.21 +/- 1.08 in group A, which was improved by 3.92 +/- 1.33; and it was 13.14 +/- 2.32 in group B, which was improved by 3.12 +/- 1.95. The average postoperative difference between anterior height and posterior height of vertebral body in group A was (3.11 +/- 1.06) mm, which was improved by (18.03 +/- 2.14) mm; and it was (2.56 +/- 1.33) mm in group B, which was corrected by (20.36 +/- 3.78) mm. There were statistically significant differences in the above indexes between preoperation and postoperation in 2 groups (P < 0.01), but no significant differences between 2 groups (P > 0.05). In group A, pleural effusion occurred in 2 cases and local pulmonary collapse in 4 cases and intercostals neuralgia in 1 case. In group B, leakage of cerebrospinal fluid occurred in 3 cases. CONCLUSION: Both anterior and posterior approach are capable of treating of the old thoracolumbar fracture with incomplete spinal cord injury and providing the satisfying result of deformation correction, neurological decompression and neurological functional recovery to a certain extent.