RESUMO
OBJECTIVE: To investigate correlation between part of histocompatibility leukocyte antigen (HLA) class II gene and gestational diabetes mellitus (GDM). METHODS: Part of HLA-II gene was examined by polymerase chain reaction sequence specific primer (PCR-SSP). Forty-eight GDM women were served as study group, and forty-eight normal pregnant women were selected as control group. RESULTS: The frequencies of DRB1*0301, DRB1*1302 and DQA1*0301 of GDM (18.8%, 45.8% and 64.6%) in study group were significantly increased compared with control group (4.2%, 18.8% and 41.7%) (P < 0.05). CONCLUSIONS: The results suggest that there is correlation between HLA class II gene and gestational diabetes mellitus. DRB1*0301, DRB1*1302 and DQA1*0301 are the susceptible alleles of GDM.
Assuntos
Diabetes Gestacional/genética , Predisposição Genética para Doença , Antígenos HLA-D/genética , Adulto , Alelos , Feminino , Frequência do Gene , Antígenos HLA-DQ/genética , Humanos , Razão de Chances , Reação em Cadeia da Polimerase/métodos , Gravidez , Terceiro Trimestre da GravidezRESUMO
OBJECTIVE: To study the anti-endotoxin of different concentration baicalin. METHODS: 6.250 microg/mL, 3.125 microLg/mL, 1.562 microg/mL and 0.781 microg/mL baicalin solutions were mixed with I EU/mL endotoxin, respectively. The mixtures were put into water of (37+/-1) degrees C for 15 min, 30 min and 60 min. The degrading effects were determined by using limulus amebocyte lysate test (LAL test). RESULTS: 1) The degrading effect of 6.250 microg/mL, 3.125 microg/mL and 1.562 microg/mL baicalin solution on I EU/mL endotoxin was degraded completely in 15 min, 30 min and 60 min, respectively. 2)The degrading effect of 3.125 microg/mL, 1.562 microg/mL and 0.781 microg/mL baicalin solution on 1 EU/mL endotoxin after these mixtures had been incubated for 15 min. Endotoxin values were (0.155 5 +/- 0.002 8) EU/mL, (0.212 1+/-0.004 9) EU/mL, (0.355 9+/-0.013 9) EU/mL, respectively. These differences among them were statistically significant (P<0.01). 3) The degrading effect of 1.562 microg/mL and 0.781 microg/mL baicalin solution on 1 EU/mL endotoxin after these mixtures had been incubated for 30 min. Endotoxin values were (0.1640+/-0.0025) EU/mL and (0.2094+/-0.004 4) EU/mL, respectively. These differences between them were statistically significant (P<0.01). CONCLUSION: The action of anti-endotoxin of baicalin is dose-dependent and time-dependent. The results show that baicalin has the stronger anti-endotoxin effect.