Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 31
Filtrar
Mais filtros

Base de dados
País/Região como assunto
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
BMC Vet Res ; 20(1): 390, 2024 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-39227948

RESUMO

BACKGROUND: This study aimed to identify the roles of L-tryptophan (Trp) and its rate-limiting enzymes on the receptivity of bovine endometrial epithelial cells. Real-time PCR was conducted to analyze the differential expression of genes between different groups of bovine endometrial epithelial cells. Western blot was performed to detect Cyclooxygenase-2 (COX2) expression after treatment with Trp or kynurenine (the main metabolites of Trp). The kynurenine assay was used to examine if Trp or prostaglandin E2 (PGE2) can increase the production of kynurenine in the bovine endometrial epithelial cells. RESULTS: Trp significantly stimulates insulin growth factor binding protein 1 (IGFBP1) expression, a common endometrial marker of conceptus elongation and uterus receptivity for ruminants. When bovine endometrial epithelial cells are treated with Trp, tryptophan hydroxylase-1 remains unchanged, but tryptophan 2,3-dioxygenase 2 (TDO2) is significantly increased, suggesting tryptophan is mainly metabolized through the kynurenine pathway. Kynurenine significantly stimulates IGFBP1 expression. Furthermore, Trp and kynurenine significantly increase the expression of aryl hydrocarbon receptor (AHR). CH223191, an AHR inhibitor, abrogates the induction of Trp and kynurenine on IGFBP1. PGE2 significantly induces the expression of TDO2, AHR, and IGFBP1. CONCLUSIONS: The regulation between Trp / kynurenine and PGE2 may be crucial for the receptivity of the bovine uterus.


Assuntos
Endométrio , Células Epiteliais , Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina , Cinurenina , Receptores de Hidrocarboneto Arílico , Triptofano Oxigenase , Triptofano , Animais , Bovinos , Feminino , Triptofano/farmacologia , Triptofano/metabolismo , Endométrio/metabolismo , Endométrio/efeitos dos fármacos , Células Epiteliais/metabolismo , Células Epiteliais/efeitos dos fármacos , Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina/metabolismo , Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina/genética , Receptores de Hidrocarboneto Arílico/metabolismo , Receptores de Hidrocarboneto Arílico/genética , Cinurenina/metabolismo , Cinurenina/farmacologia , Triptofano Oxigenase/metabolismo , Triptofano Oxigenase/genética , Dinoprostona/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Ciclo-Oxigenase 2/metabolismo , Ciclo-Oxigenase 2/genética
2.
Opt Lett ; 48(23): 6156-6159, 2023 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-38039215

RESUMO

A challenge in all-fiber-integrated metasurface devices is to efficiently control dispersion in the limited fiber end area to build metasurfaces, therefore, the design of metasurfaces with a special structure becomes crucial to meet the demands of dispersion control. A unique phase response of circularly polarized light in catenary metasurfaces can offer new opportunities for polarization-sensitive arbitrary chromatic dispersion control. Herein, we proposed an optical achromatic metalens based on equal width catenary metasurfaces integrated on the large-mode optical fiber (LMF) end. To reduce phase distortions, the LMF is designed to generate quasi-plane waves (QPW), and then QPW converts from catenary metasurfaces to realize achromatic focusing. A notable feature of this device is its axial focal length shift as low as 0.09% across the working wavelength range from 1.33 µm to 1.55 µm, commonly used in optical fiber communication, demonstrating its excellent dispersion control capability. Furthermore, the device exhibits exceptional capabilities to break through the diffraction limit of the output field. This research has potential applications in the fields of achromatic devices, chromatic aberration correction, fiber lasers, and optical communication and modulation.

3.
Phys Chem Chem Phys ; 25(5): 3681-3694, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36650982

RESUMO

Understanding the propagation of dynamic wrinkles in polycrystalline graphene with grain boundaries (GBs) is critical to the practical application of graphene-based nanodevices. Although wrinkle propagation behavior in pristine graphene (PG) and some defect-containing graphene samples have been investigated, there are no studies on the dynamic behavior of graphene with tilt GBs. Here, nine tilt GBs are constructed in graphene, and molecular dynamics (MD) simulations are performed to investigate anomalous wrinkle propagation. The MD simulation results show that a larger misorientation angle α first enhances the shielding effect of tilt GBs on wrinkle propagation before it weakens. The maximum Δz root mean square (RMS) shows that a greater misorientation angle α first increases the maximum RMS of the GB region (RGB) before it then decreases, while the maximum RMS of R80 exhibits the opposite trend. Moreover, approximately 96% of the C60 kinetic energy is converted into kinetic and potential energies in graphene, and the potential energy in graphene presents two evolution modes. Phase diagrams are plotted to study the effect of the distance d1 and rotation angle ß on the wrinkle propagation and sensitivity of the maximum RMS value to d1. It is expected that our results can provide a fundamental understanding of defect engineering and guidelines to design protectors, energy absorbers, and defect detectors in nanodevices.

4.
Environ Geochem Health ; 45(11): 7775-7789, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37432490

RESUMO

Heavy metal pollution in agricultural soil is a threat to people's health and sustainable development. However, there is currently no nationwide health risk assessment in China. In this study, we performed a preliminary assessment of heavy metals in agricultural soils of the Chinese mainland, and found obvious carcinogenic risks (total lifetime carcinogenic risk (TLCR) > 1 × 10-5). A similar spatial distribution pattern was found in soil heavy metal and the mortality of esophagus and stomach cancers. Combining the potential carcinogenic risk assessed by LCR for individual heavy metal with Pearson correlation, Geographical Detector (q statistic > 0.75 for TLCR, p < 0.05), and redundancy analysis (RDA), it was found that long-term exposure and intake route of heavy metals exceeding the maximum safety threshold (Health Canada standard) may induce digestive system (esophagus, stomach, liver, and colorectum) cancers in rural populations. Through Partial Least Squares Path Model (PLS-PM), it was also revealed that the LCR of heavy metals was closely related to the soil environmental background (path coefficients = 0.82), which in turn was affected by factors such as economic development and pollution discharge. The current research results highlight the potential carcinogenic risk to the digestive system associated with low-dose and long-term exposure to heavy metals in agricultural soils, and policymakers should propose countermeasures and solutions according to the local conditions.


Assuntos
Metais Pesados , Poluentes do Solo , Humanos , Monitoramento Ambiental , Solo , Poluentes do Solo/toxicidade , Poluentes do Solo/análise , Metais Pesados/toxicidade , Metais Pesados/análise , China/epidemiologia , Carcinógenos , Medição de Risco
5.
Mol Microbiol ; 116(4): 1216-1231, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34494331

RESUMO

Uropathogenic Escherichia coli (UPEC) is the primary causative agent of urinary tract infections (UTIs). Successful urinary tract colonization requires appropriate expression of virulence factors in response to host environmental cues, such as limited oxygen and iron availability. Hemolysin is a pore-forming toxin, and its expression correlates with the severity of UPEC infection. Previously, we showed that hemolysin expression is enhanced under anaerobic conditions; however, the genetic basis and regulatory mechanisms involved remain undefined. Here, a transposon-based forward screen identified bis-molybdopterin guanine dinucleotide cofactor (bis-MGD) biosynthesis as an important factor for a full transcription of hemolysin under anaerobiosis but not under aerobiosis. bis-MGD positively influences hemolysin transcription via c3566-c3568, an operon immediately upstream of and cotranscribed with hlyCABD. Furthermore, suppressor mutation analysis identified the nitrogen regulator NtrC as a direct repressor of c3566-c3568-hlyCABD expression, and intact bis-MGD biosynthesis downregulated ntrC expression, thus at least partially explaining the positive role of bis-MGD in modulating hemolysin expression. Finally, bis-MGD is involved in hemolysin-mediated uroepithelial cell death and contributes to the competitive fitness of UPEC in a murine model of UTI. Collectively, our data establish that bis-MGD biosynthesis plays a crucial role in UPEC fitness in vivo, thus providing a potential target for combatting UTIs.


Assuntos
Infecções por Escherichia coli/microbiologia , Nucleotídeos de Guanina/metabolismo , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Pterinas/metabolismo , Escherichia coli Uropatogênica/genética , Escherichia coli Uropatogênica/metabolismo , Anaerobiose , Animais , Morte Celular , Linhagem Celular , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Humanos , Camundongos , Camundongos Endogâmicos CBA , Mutagênese Insercional , Óperon , Proteínas PII Reguladoras de Nitrogênio/metabolismo , Fatores de Transcrição/metabolismo , Transcriptoma , Virulência , Fatores de Virulência/genética , Fatores de Virulência/metabolismo
6.
Phys Chem Chem Phys ; 23(20): 11917-11930, 2021 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-33998625

RESUMO

This study investigated dynamic surface wrinkle propagation across a series of flower-like rotational grain boundaries (GBs) in graphene using theoretical solutions and atomistic simulations. It was found that there was significantly less out-of-plane displacement of dynamic wrinkles when curvature of rotational GBs was reduced, which can be explained by a defect shielding effect of flower-like GBs. Potential energy evolved via different modes for pristine graphene and graphene with various GBs. With external excitation, the distinctly different patterns of wrinkle propagation in graphene with various GBs demonstrated how dynamic wrinkling can reveal defects. These results can provide a theoretical basis for guiding the design and implementation of graphene-based nano-mechanical devices such as protectors and detectors.

7.
J Cell Mol Med ; 23(4): 2558-2567, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30701683

RESUMO

Diabetic cardiomyopathy (DCM) is a condition associated with significant structural changes including cardiac tissue necrosis, localized fibrosis, and cardiomyocyte hypertrophy. This study sought to assess whether and how FBXL10 can attenuate DCM using a rat streptozotocin (STZ)-induced DCM model system. In the current study, we found that FBXL10 expression was significantly decreased in diabetic rat hearts. FBXL10 protected cells from high glucose (HG)-induced inflammation, oxidative stress, and apoptosis in vitro. In addition, FBXL10 significantly activated PKC ß2 signaling pathway in H9c2 cells and rat model. The cardiomyocyte-specific overexpression of FBXL10 at 12 weeks after the initial STZ administration attenuated oxidative stress and inflammation, thereby reducing cardiomyocyte death and preserving cardiac function in these animals. Moreover, FBXL10 protected against DCM via activation of the PKC ß2 pathway. In conclusion, FBXL has the therapeutic potential for the treatment of DCM.


Assuntos
Diabetes Mellitus Experimental/genética , Cardiomiopatias Diabéticas/genética , Proteínas F-Box/genética , Glucose/farmacologia , Miócitos Cardíacos/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-akt/genética , Animais , Apoptose/efeitos dos fármacos , Apoptose/genética , Técnicas de Cultura de Células , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/fisiopatologia , Cardiomiopatias Diabéticas/induzido quimicamente , Cardiomiopatias Diabéticas/metabolismo , Cardiomiopatias Diabéticas/fisiopatologia , Proteínas F-Box/metabolismo , Regulação da Expressão Gênica , Glucose/metabolismo , Hemodinâmica , Masculino , Miócitos Cardíacos/citologia , Miócitos Cardíacos/metabolismo , Estresse Oxidativo , Cultura Primária de Células , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Estreptozocina
8.
Med Phys ; 51(2): 1145-1162, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37633838

RESUMO

BACKGROUND: Magnetic resonance imaging (MRI) is the preferred imaging modality for diagnosing knee disease. Segmentation of the knee MRI images is essential for subsequent quantification of clinical parameters and treatment planning for knee prosthesis replacement. However, the segmentation remains difficult due to individual differences in anatomy, the difficulty of obtaining accurate edges at lower resolutions, and the presence of speckle noise and artifacts in the images. In addition, radiologists must manually measure the knee's parameters which is a laborious and time-consuming process. PURPOSE: Automatic quantification of femoral morphological parameters can be of fundamental help in the design of prosthetic implants for the repair of the knee and the femur. Knowledge of knee femoral parameters can provide a basis for femoral repair of the knee, the design of fixation materials for femoral prostheses, and the replacement of prostheses. METHODS: This paper proposes a new deep network architecture to comprehensively address these challenges. A dual output model structure is proposed, with a high and low layer fusion extraction feature module designed to extract rich features through the cross-fusion mechanism. A multi-scale edge information extraction spatial feature module is also developed to address the boundary-blurring problem. RESULTS: Based on the precise automated segmentation results, 10 key clinical parameters were automatically measured for a knee femoral prosthesis replacement program. The correlation coefficients of the quantitative results of these parameters compared to manual results all achieved at least 0.92. The proposed method was extensively evaluated with MRIs of 78 patients' knees, and it consistently outperformed other methods used for segmentation. CONCLUSIONS: The automated quantization process produced comparable measurements to those manually obtained by radiologists. This paper demonstrates the viability of automatic knee MRI image segmentation and quantitative analysis with the proposed method. This provides data to support the accuracy of assessing the progression and biomechanical changes of osteoarthritis of the knee using an automated process, thus saving valuable time for the radiologists and surgeons.


Assuntos
Processamento de Imagem Assistida por Computador , Articulação do Joelho , Humanos , Processamento de Imagem Assistida por Computador/métodos , Articulação do Joelho/diagnóstico por imagem , Joelho/diagnóstico por imagem , Imageamento por Ressonância Magnética/métodos , Fêmur/diagnóstico por imagem
9.
Front Hum Neurosci ; 18: 1342931, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38681742

RESUMO

Objectives: The auditory spatial processing abilities mature throughout childhood and degenerate in older adults. This study aimed to compare the differences in onset cortical auditory evoked potentials (CAEPs) and location-evoked acoustic change complex (ACC) responses among children, adults, and the elderly and to investigate the impact of aging and development on ACC responses. Design: One hundred and seventeen people were recruited in the study, including 57 typically-developed children, 30 adults, and 30 elderlies. The onset-CAEP evoked by white noise and ACC by sequential changes in azimuths were recorded. Latencies and amplitudes as a function of azimuths were analyzed using the analysis of variance, Pearson correlation analysis, and multiple linear regression model. Results: The ACC N1'-P2' amplitudes and latencies in adults, P1'-N1' amplitudes in children, and N1' amplitudes and latencies in the elderly were correlated with angles of shifts. The N1'-P2' and P2' amplitudes decreased in the elderly compared to adults. In Children, the ACC P1'-N1' responses gradually differentiated into the P1'-N1'-P2' complex. Multiple regression analysis showed that N1'-P2' amplitudes (R2 = 0.33) and P2' latencies (R2 = 0.18) were the two most variable predictors in adults, while in the elderly, N1' latencies (R2 = 0.26) explained most variances. Although the amplitudes of onset-CAEP differed at some angles, it could not predict angle changes as effectively as ACC responses. Conclusion: The location-evoked ACC responses varied among children, adults, and the elderly. The N1'-P2' amplitudes and P2' latencies in adults and N1' latencies in the elderly explained most variances of changes in spatial position. The differentiation of the N1' waveform was observed in children. Further research should be conducted across all age groups, along with behavioral assessments, to confirm the relationship between aging and immaturity in objective ACC responses and poorer subjective spatial performance. Significance: ACCs evoked by location changes were assessed in adults, children, and the elderly to explore the impact of aging and development on these differences.

10.
Food Funct ; 15(8): 4140-4153, 2024 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-38445991

RESUMO

Milk glycans play key roles in shaping and maintaining a healthy infant gut microbiota. Core fucosylation catalyzed by fucosyltransferase (Fut8) is the major glycosylation pattern on human milk N-glycan, which was crucial for promoting the colonization and dominant growth of Bifidobacterium and Lactobacillus spp. in neonates. However, the influence of core-fucose in breast milk on the establishment of early-life immune tolerance remains poorly characterized. In this study, we found that the deficiency of core-fucose in the milk of maternal mice caused by Fut8 gene heterozygosity (Fut8+/-) resulted in poor immune tolerance towards the ovalbumin (OVA) challenge, accompanied by a reduced proportion of intestinal RORγt+ Treg cells and the abundance of Lactobacillus spp., especially L. reuteri and L. johnsonii, in their breast-fed neonates. The administration of the L. reuteri and L. johnsonii mixture to neonatal mice compromised the OVA-induced allergy and up-regulated the intestinal RORγt+ Treg cell proportions. However, Lactobacillus mixture supplementation did not alleviate allergic responses in RORγt+ Treg cell-deficient mice caused by Rorc gene heterozygosity (Rorc+/-) post OVA challenge, indicating that the intervention effects depend on the RORγt+ Treg cells. Interestingly, instead of L. reuteri and L. johnsonii, we found that the relative abundance of another Lactobacillus spp., L. murinus, in the gut of the offspring mice was significantly promoted by intervention, which showed enhancing effects on the proliferation of splenic and intestinal RORγt+ Treg cells in in vitro studies. The above results indicate that core fucosylation of breast milk N-glycans is beneficial for the establishment of RORγt+ Treg cell mediated early-life immune tolerance through the manipulation of symbiotic bacteria in mice.


Assuntos
Microbioma Gastrointestinal , Tolerância Imunológica , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares , Polissacarídeos , Linfócitos T Reguladores , Animais , Linfócitos T Reguladores/imunologia , Camundongos , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , Feminino , Polissacarídeos/metabolismo , Lactobacillus , Fucosiltransferases/metabolismo , Fucosiltransferases/genética , Leite Humano/imunologia , Humanos , Fucose/metabolismo , Animais Recém-Nascidos , Camundongos Endogâmicos C57BL , Leite
11.
Biochem Biophys Rep ; 38: 101654, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38375420

RESUMO

Spinal cord injury (SCI) is a disturbance of peripheral and central nerve conduction that causes disability in sensory and motor function. Currently, there is no effective treatment for SCI. Mitophagy plays a vital role in mitochondrial quality control during various physiological and pathological processes. The study aimed to elucidate the role of mitophagy and identify potential mitophagy-related hub genes in SCI pathophysiology. Two datasets (GSE15878 and GSE138637) were analyzed. Firstly, the differentially expressed genes (DEGs) were identified and mitophagy-related genes were obtained from GeneCards, then the intersection between SCI and mitophagy-related genes was determined. Next, we performed gene set enrichment analysis (GSEA), weighted gene co-expression network analysis (WGCNA), protein-protein interaction network (PPI network), least absolute shrinkage and selection operator (LASSO), and cluster analysis to identify and define the hub genes in SCI. Finally, the link between hub genes and infiltrating immune cells was investigated and the potential transcriptional regulation/small molecular compounds to target hub genes were predicted. In total, SKP1 and BAP1 were identified as hub genes of mitophagy-related DEGs during SCI development and regulatory T cells (Tregs)/resting NK cells/activated mast cells may play an essential role in the progression of SCI. LINC00324 and SNHG16 may regulate SKP1 and BAP1, respectively, through miRNAs. Eleven and eight transcriptional factors (TFs) regulate SKP1 and BAP1, respectively, and six small molecular compounds target BAP1. Then, the mRNA expression levels of BAP1 and SKP1 were detected in the injured sites of spinal cord of SD rats at 6 h and 72 h after injury using RT-qPCR, and found that the level were decreased. Therefore, the pathways of mitophagy are downregulated during the pathophysiology of SCI, and SKP1 and BAP1 could be accessible targets for diagnosing and treating SCI.

12.
Theriogenology ; 229: 16-22, 2024 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-39142066

RESUMO

Spermatogenesis in eukaryotes is a process that occurs within a very narrow temperature threshold, typically not exceeding 36 °C. SPO11 was isolated from the temperature-sensitive mutant receptor of Saccharomyces cerevisiae and is thought to be the only protein that functions during meiosis. This suggested that SPO11 may be the key protein that influenced the temperature of spermatogenesis not exceeding 36 °C. Elevated temperatures typically damage the spermatogenic cells. Birds have a core body temperature of 41-42 °C, and their testis are located inside their bodies, providing an alternative perspective to investigate the potential impact of temperature threshold on spermatogenesis. The objective of this study was to ascertain whether elevated ambient temperatures affect spermatogenesis in birds and whether SPO11 is the key gene affecting the temperature threshold for spermatogenesis. STRA8, SCP3, SPO11, γ-H2AX, and RAD51 were all crucial components in the process of meiotic initiation, synapsis, DNA double-strand break (DSB) induction, homologous chromosome crossover recombination, and repair of DSB. In this study, 39-day-old Japanese quail were subjected to heat stress (HS) at 38 °C for 8 h per day for 3 (3d HS) and 13 (13d HS) consecutive days and analyzed the expression of meiotic signaling molecules (STRA8, SCP3, SPO11, γ-H2AX, and RAD51) using molecular biology techniques, including Immunohistochemistry (IHC), Western Blot (WB), and Real-time Quantitative Polymerase Chain Reaction (qRT-PCR). We found that spermatogenesis was normal in both groups exposed to HS. Meiotic signaling molecules were expressed normally in the 3d HS group. All detected signaling molecules were normally expressed in the 13d HS group, except for SPO11, which showed a significant increase in expression, indicating that SPO11 was temperature-sensitive. We examined the localized expression of each meiotic signaling molecule in quail testis, explored the temperature sensitivity of SPO11, and determined that quail testis can undergo normal spermatogenesis at ambient temperatures exceeding 36 °C. This study concluded that SPO11 is not the key protein influencing spermatogenesis in birds. These findings enhance our understanding of avian spermatogenesis.

13.
Biol Direct ; 19(1): 72, 2024 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-39175035

RESUMO

BACKGROUND: TSPAN7 is an important factor in tumor progression. However, the precise function of TSPAN7 and its role in pan-cancer are not clear. METHODS: Based on Xinhua cohort incorporating 370 patients with kidney neoplasm, we conducted differential expression analysis by immunohistochemistry between tumor and normal tissues, and explored correlations of TSPAN7 with patients' survival. Subsequently, we conducted a pan-cancer study, and successively employed differential expression analysis, competing endogenous RNA (ceRNA) analysis, protein-protein interaction (PPI) analysis, correlation analysis of TSPAN7 with clinical characteristics, tumor purity, tumor genomics, tumor immunity, and drug sensitivity. Last but not least, gene set enrichment analysis was applied to identify enriched pathways of TSPAN7. RESULTS: In Xinhua cohort, TSPAN7 expression was significantly up-regulated (P-value = 0.0019) in tumor tissues of kidney neoplasm patients. High TSPAN7 expression was associated with decreases in overall survival (OS) (P-value = 0.009) and progression-free survival (P-value = 0.009), and it was further revealed as an independent risk factor for OS (P-value = 0.0326, HR = 5.66, 95%CI = 1.155-27.8). In pan-cancer analysis, TSPAN7 expression was down-regulated in most tumors, and it was associated with patients' survival, tumor purity, tumor genomics, tumor immunity, and drug sensitivity. The ceRNA network and PPI network of TSPAN7 were also constructed. Last but not least, the top five enriched pathways of TSPAN7 in various tumors were identified. CONCLUSION: TSPAN7 served as a promising biomarker of various tumors, especially kidney neoplasms, and it was closely associated with tumor purity, tumor genomics, tumor immunology, and drug sensitivity in pan-cancer level.


Assuntos
Biologia Computacional , Neoplasias Renais , Tetraspaninas , Humanos , Tetraspaninas/genética , Tetraspaninas/metabolismo , Biologia Computacional/métodos , Neoplasias Renais/genética , Neoplasias Renais/metabolismo , Estudos Retrospectivos , Masculino , Feminino , Neoplasias/genética , Neoplasias/metabolismo , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Pessoa de Meia-Idade , Regulação Neoplásica da Expressão Gênica , Prognóstico , Proteínas do Tecido Nervoso
14.
Viruses ; 16(8)2024 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-39205307

RESUMO

The study aimed to analyze changes in the clinical and epidemiological aspects of HIV-associated cryptococcal meningitis (CM) patients and to identify factors influencing their prognosis. Clinical data of patients with HIV-associated CM treated in Shanghai, China between 2013 and 2023 were collected. This study included 279 cases, 2.89% of AIDS patients, showing a yearly decrease in CM prevalence among AIDS patients (p < 0.001). Overall mortality was 10.39% with rates declining from a 2013 peak of 15.38% to 0% in 2023 despite no significant temporal pattern (p = 0.265). Diagnosis took an average of 18 ± 1 days post-symptoms, and admission CD4 counts averaged 29.2 ± 2.5 cells/µL, hinting at a non-significant decline. Frequent symptoms included fever (62.4%), headache (61.6%), fatigue (44.1%), and appetite loss (39.8%), with younger patients more likely to initially show signs of meningeal irritation. Logistic regression analysis underscored the prognostic importance of cerebrospinal fluid (CSF) white blood cell (WBC) count and procalcitonin levels. Over the decade spanning from 2013 to 2023, the incidence and mortality rates of CM among AIDS patients exhibited a downward trend. The average duration from the onset of CM to confirmation of diagnosis remained prolonged. CSF WBC count and procalcitonin levels were associated with unfavorable outcomes.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS , Infecções por HIV , Meningite Criptocócica , Humanos , Meningite Criptocócica/epidemiologia , Meningite Criptocócica/mortalidade , China/epidemiologia , Feminino , Masculino , Adulto , Pessoa de Meia-Idade , Infecções por HIV/complicações , Infecções por HIV/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/mortalidade , Prognóstico , Contagem de Linfócito CD4 , Prevalência , Incidência , Adulto Jovem
15.
Theor Appl Genet ; 126(12): 3081-9, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24061485

RESUMO

KEY MESSAGE: The effectiveness of wheat cultivar Liangxing 99 against powdery mildew was shown to be controlled by a single dominant gene located on a new locus of chromosome 2BL in the bin 2BL2-0.35-0.50. Liangxing 99, one of the most widely grown commercial cultivars in the winter wheat (Triticum aestivum) producing regions in northern China, was shown to provide a broad spectrum of resistance to Blumeria graminis f. sp. tritici (Bgt) isolates originating from that region. Using an F2 population and F2:3 lines derived from a cross of Liangxing 99 × Zhongzuo 9504, genetic analysis demonstrated that a single dominant gene, designated MlLX99, was responsible for the resistance of Liangxing 99 to Bgt isolate E09. The results of molecular analysis indicated that this gene is located on chromosome 2BL and flanked by the SSR marker Xgwm120 and EST-STS marker BE604758 at genetic distances of 2.9 and 5.5 cM, respectively. Since the flanking markers of MlLX99 were previously mapped to the bin 2BL2-0.36-0.50, MlLX99 must be located in this chromosomal region. MlLX99 showed a different resistance reaction pattern to 60 Bgt isolates from Pm6, Pm33, and PmJM22, which were all previously mapped on chromosome 2BL, but differed in their positions from MlLX99. Due to its unique position on chromosome 2BL, MlLX99 appears to be a new locus for resistance to powdery mildew. Liangxing 99 has shown superior yield performance and wide adaptation to different agricultural conditions, which has resulted in its extensive use as a wheat cultivar in China. The identification of resistance gene MlLX99 facilitates the use of this cultivar in the protection of wheat from damage caused by powdery mildew.


Assuntos
Ascomicetos/fisiologia , Mapeamento Cromossômico , Resistência à Doença/genética , Genes de Plantas/genética , Imunidade Inata/genética , Doenças das Plantas/genética , Triticum/genética , Cromossomos de Plantas/genética , DNA de Plantas/genética , Ligação Genética , Marcadores Genéticos/genética , Doenças das Plantas/imunologia , Doenças das Plantas/microbiologia , Triticum/imunologia , Triticum/microbiologia
16.
J Chromatogr A ; 1690: 463787, 2023 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-36652815

RESUMO

Alectinib and its metabolite, M4, have demonstrated a satisfactory clinical therapeutic effect in the treatment of anaplastic lymphoma kinase-positive advanced non-small-cell lung cancer. Due to individual differences among patients, therapeutic drug monitoring (TDM) is critical for guaranteeing appropriate clinical drug use. To realize TDM for alectinib and its metabolite, M4, a honeycomb phenol-formaldehyde resin (PFR) with excellent hydrophilic properties, abundant adsorption force, and a stable porous structure was synthesized by modifying the porogens F127 and P123. The prepared PFR was employed as an adsorbent in a simple and efficient spin-column solid-phase extraction (SPE) process. A rapid method for detecting alectinib and its metabolite M4 in urine was thereby established. The established method showed a linear range of 0.0200 µg mL-1-5.00 µg mL-1 and the recovery range of 98.8-103% for spiked urine samples, with relative standard deviations of ≤ 4.87% (n = 3). Our results proved the practicability of the proposed honeycomb-PFR spin-column SPE method in TDM for alectinib and its metabolite, M4.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Humanos , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Piperidinas , Extração em Fase Sólida
17.
Neural Netw ; 162: 359-368, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36940496

RESUMO

Most multi-agent reinforcement learning (MARL) approaches optimize strategy by improving itself, while ignoring the limitations of homogeneous agents that may have single function. However, in reality, the complex tasks tend to coordinate various types of agents and leverage advantages from one another. Therefore, it is a vital research issue how to establish appropriate communication among them and optimize decision. To this end, we propose a Hierarchical Attention Master-Slave (HAMS) MARL, where the Hierarchical Attention balances the weight allocation within and among clusters, and the Master-Slave architecture endows agents independent reasoning and individual guidance. By the offered design, information fusion, especially among clusters, is implemented effectively, and excessive communication is avoided, moreover, selective composed action optimizes decision. We evaluate the HAMS on both small and large scale heterogeneous StarCraft II micromanagement tasks. The proposed algorithm achieves the exceptional performance with more than 80% win rates in all evaluation scenarios, which obtains an impressive win rate of over 90% in the largest map. The experiments demonstrate a maximum improvement in win rate of 47% over the best known algorithm. The results show that our proposal outperforms recent state-of-the-art approaches, which provides a novel idea for heterogeneous multi-agent policy optimization.


Assuntos
Aprendizagem , Reforço Psicológico , Resolução de Problemas , Algoritmos , Comunicação
18.
Environ Technol ; 44(10): 1415-1425, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34779745

RESUMO

Pig farm biogas slurry is being increasingly used as a potent organic fertilizer for sustainable agriculture under circular economy. However, the effect of biogas slurry on soil pH is currently controversial, and the underlying mechanisms especially in saline-alkali soils are not well understood. A saline-alkali soil (pH = 9.2, EC = 2.0 ms/cm) was selected for soil column (0-50 cm) experiments with (BS) and without (CK) addition of pig farm biogas slurry to investigate the soil pH change and its driving factors. Our results show that the soil pH under CK ranged between 9.1 and 9.5 across different soil depths. Compared to CK, the BS-treated soil had lower pH at 0-20 cm depth and higher pH at 20-30 cm depth (P < 0.01). The soil NH4+-N concentrations were negatively correlated with pH values under BS (P < 0.01), indicating that the oxidation of ammonium mainly contributed to the decrease of soil pH. Interestingly, the anions, such as Cl-, SO42- and NO3-, were accumulated in the topsoil (0-20 cm) under BS, resulting in the changed correlations of these anions with Na+ when compared to the control. FT-IR and 13C-NMR spectra uncovered that carboxyl, amide C, and total alkyl C groups may be responsible for reducing pH of the saline-alkali soil tested. The soil surface morphology confirmed a much tighter granular aggregate microstructure when mixing the biogas slurry with the soil. Overall, we concluded that from the perspective of soil pH, the utilization of biogas slurry for improving saline-alkali soil is feasible and sustainable.


Assuntos
Biocombustíveis , Solo , Animais , Suínos , Solo/química , Álcalis , Fazendas , Espectroscopia de Infravermelho com Transformada de Fourier , Fertilizantes/análise , Ânions , Concentração de Íons de Hidrogênio
19.
J Chromatogr A ; 1705: 464224, 2023 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-37490816

RESUMO

Osimertinib (OSIM) is widely used as a mainstream drug for the treatment of non-small cell lung cancer (NSCLC). However, the lack of a rapid extraction and detection method for OSIM and its metabolite, AZ-5104, has limited clinical drug metabolism and drug resistance research because the drug is unstable. In this study, a new ionic liquid hybrid hierarchical porous material (IL-HHPM) was synthesized with hierarchical porous structures, including micropores (1.6-2.0 nm), mesopores (2.0-50.0 nm), macropores (50.0-148.7 nm), and multiple functional groups via a one-step hydrothermal method using silanized ionic liquids (IL) as functionalized hybrid monomer. The IL-HHPM has the advantages of a high specific surface area (437.4 ± 4.6 m2 g-1), sizable pore volume (0.74 cm3 g-1), and fast mass transfer, additionally, the IL-HHPM adsorbed OSIM and AZ-5104 via π-π interactions and hydrogen bonding. OSIM and AZ-5104 were rapidly extracted and measured in human urine using rapid and miniaturized centrifugal spin-column extraction (MCSCE), which was based on the IL-HHPM. The optimized factors for the extraction recoveries of OSIM and AZ-5104 were adsorbent dosage (8.0 mg), sample volume (0.5 mL), and operation time (9.0 min), and markedly reduced the adsorbent dosage and operation time. The IL-HHPM-MCSCE-HPLC method displayed good linearity (0.02-5.00 µg mL-1, r ≥ 0.9997), satisfying accuracy (spiked recoveries of 87.7%-100.0%), and good precision (RSDs ≤ 7.0%). The developed method is rapid, sensitive, and reproducible for the simultaneous determination of trace level of OSIM and AZ-5104 in human urine.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Líquidos Iônicos , Neoplasias Pulmonares , Humanos , Líquidos Iônicos/química , Porosidade , Cromatografia Líquida de Alta Pressão/métodos , Extração em Fase Sólida/métodos
20.
Carbohydr Polym ; 321: 121308, 2023 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-37739538

RESUMO

Cellulose-based conductive composite fibers hold great promise in smart wearable applications, given cellulose's desirable properties for textiles. Blending conductive fillers with cellulose is the most common means of fiber production. Incorporating a high content of conductive fillers is demanded to achieve desirable conductivity. However, a high filler load deteriorates the processability and mechanical properties of the fibers. Here, developing wet-spun cellulose-based fibers with a unique side-by-side (SBS) structure via sustainable processing is reported. Sustainable sources (cotton linter and post-consumer cotton waste) and a biocompatible intrinsically conductive polymer (i.e., polyaniline, PANI) were engineered into fibers containing two co-continuous phases arranged side-by-side. One phase was neat cellulose serving as the substrate and providing good mechanical properties; another phase was a PANI-rich cellulose blend (50 wt%) affording electrical conductivity. Additionally, an eco-friendly LiOH/urea solvent system was adopted for the fiber spinning process. With the proper control of processing parameters, the SBS fibers demonstrated high conductivity and improved mechanical properties compared to single-phase cellulose and PANI blended fibers. The SBS fibers demonstrated great potential for wearable e-textile applications.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA