RESUMO
Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was beneficial to angiogenesis, tissue repair, and regeneration. The adhesion of MSCs and EPCs could promote the pluripotency of MSCs, particularly self-renewal and multi-differentiation to osteoblasts, chondrocytes, and adipocytes. This study focused on the mechanism of adhesion between EPCs and MSCs. The results showed that E-cadherin (E-cad) mediated the adhesion of MSCs and EPCs through the E-cad/beta-catenin signaling pathway. The E-cad of EPCs occupied a dominant position during this process, which activated and up-regulated the beta-catenin (ß-catenin) of MSCs to improve cohesion and exert their biological function.
Assuntos
Caderinas/metabolismo , Células Progenitoras Endoteliais/metabolismo , Células-Tronco Mesenquimais/metabolismo , Transdução de Sinais , beta Catenina/metabolismo , Animais , Células da Medula Óssea/citologia , Caderinas/antagonistas & inibidores , Caderinas/genética , Adesão Celular , Diferenciação Celular , Células Cultivadas , Técnicas de Cocultura , Células Progenitoras Endoteliais/citologia , Ensaio de Imunoadsorção Enzimática , Células-Tronco Mesenquimais/citologia , Camundongos , Camundongos Endogâmicos C57BL , Microscopia de Fluorescência , Interferência de RNA , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Regulação para Cima , beta Catenina/genéticaRESUMO
OBJECTIVE: To investigate the incidence, mortality, and disease burden of cancer in China to provide a reference for cancer prevention and control. METHODS: Cancer registry data (2006-2010) were collected from the Chinese Cancer Registry Annual Report by the National Center for Cancer Registries. Cancer incidence and mortality, potential years of life lost (PYLL), and disability-adjusted life years (DALYs) were calculated. RESULTS: The cancer incidence rate was 267.13/100,000 overall, 299.13/100,000 in men, and 234.06/100,000 in women (1.2 times higher in men than in women). The cancer mortality rate was 176.32/100,000 overall, 220.54/100,000 in men, and 130.60/100,000 in women (1.6 times higher in men than in women). The incidence rate was higher in urban areas (285.97/100,000) than in rural areas (250.91/100,000), whereas the mortality rate was higher in rural areas (179.25/100,000) than in urban areas (172.91/100,000). The rural: urban incidence ratio decreased from 0.89 in 2006 to 0.69 in 2010, and the rural: urban mortality ratio decreased from 1.10 to 0.91 in the same years. PYLL rates and DALY rates were higher in men (16.45 and 22.19, respectively) than in women (11.22 and 13.87, respectively) and in rural areas (17.6 and 22.17, respectively) than in urban areas (12.6 and 17.09, respectively). The male: female ratios for PYLL and DALY rates were 1.46 and 1.6, respectively. The rural: urban ratios for PYLL and DALY rates decreased from 1.63 in 2006 to 1.22 in 2010 and from 1.51 in 2006 to 1.08 in 2010, respectively. CONCLUSION: The disease burden of cancer in China in 2006-2010 was substantial, particularly for men and residents of rural districts.