RESUMO
Various strategies adapted to develop an efficient vaccine against foodborne pathogen, Listeria monocytogenes, have met with little success. Spheroplasts (bacterial cell devoid of cell wall) are likely to undergo membrane-membrane fusion, leading to the delivery of their content to the cytosol of antigen-presenting cells, thus facilitating MHC class I antigen processing and presentation. In this study, we evaluated the prophylactic potential of Listeria spheroplast-based vaccine against experimental murine listeriosis in comparison with heat-killed Listeria (HKL) and archaeosome-entrapped Listeria whole-cell protein (LWCP). Compared with HKL, the spheroplast-based vaccine was found to evoke better Th1 response as exhibited by the presence of type 1 cytokines in the host (interferon-γ and IL-12) and a high IgG2a /IgG1 ratio. Robust lympho-proliferative efficacy was apparent in both spheroplast-immunized and archaeosome-entrapped LWCP-immunized groups. The upregulation of costimulatory and effector memory markers upon immunization with spheroplasts was found to be at par with that evoked by archaeosome-entrapped LWCP-immunized group. Central memory response in gated CD8(+) T cell was much higher in spheroplast-immunized animals when compared with archaeosome-entrapped LWCP group. The data presented here clearly demonstrate that spheroplasts evoked a robust immune response and offer better prophylactic potential against L. monocytogenes.
Assuntos
Vacinas Bacterianas/imunologia , Imunização/métodos , Listeria monocytogenes/imunologia , Listeriose/prevenção & controle , Esferoplastos/imunologia , Animais , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/administração & dosagem , Linfócitos T CD8-Positivos/imunologia , Parede Celular/imunologia , Modelos Animais de Doenças , Feminino , Hipersensibilidade Tardia/imunologia , Switching de Imunoglobulina/imunologia , Imunoglobulina G/imunologia , Interferon gama/imunologia , Interleucina-12/imunologia , Interleucina-4/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Células Th1/imunologia , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/imunologiaAssuntos
Patos , Vírus da Hepatite B/isolamento & purificação , Neoplasias Hepáticas/veterinária , Doenças das Aves Domésticas/microbiologia , Animais , Carcinoma Hepatocelular/epidemiologia , China , Humanos , Neoplasias Hepáticas/epidemiologia , Neoplasias Hepáticas/microbiologia , Microscopia Eletrônica , Doenças das Aves Domésticas/patologiaRESUMO
Anomalous origin of the right coronary artery (RCA) from the pulmonary artery (PA) is a rare congenital anomaly, and only 28 cases have been reported in the pediatric age group. We describe the case of an infant who had progressive mitral regurgitation and papillary muscle dysfunction in association with anomalous origin of the RCA from the PA. The diagnosis was made by color flow Doppler, confirmed by angiography, and the case was successfully corrected by reimplantation of the anomalous RCA to the aorta. This is only the second case of anomalous origin of the RCA from the PA diagnosed in infancy without an associated congenital anomaly of the heart and great vessels.