Stability and morphology of Ag nanoplatelets probed by depolarized dynamic light scattering.

The stability of silver nanoplatelet (NP) suspensions prepared with different concentrations of trisodium citrate (TSC) was studied by depolarized dynamic light scattering (DDLS) and UV-vis spectrometry. The morphology of the nanoparticles, as well as the color and stability of the sols, are tuned by the concentration of the capping agent. The nanoparticles prepared with high TSC concentration (>10-4 M) are blue triangular NPs showing a slight truncation of the tips with aging. When low TSC concentrations are used, the color of the sols changes from blue to yellow with aging time and a strong modification of the morphology occurs: the nanoparticle shape changes from triangular to spherical. Remarkably, they show a high degree of anisotropy. The aging process was followed by the UV-vis spectra and by measuring the rotational diffusion coefficient by DDLS, providing information on the nanoparticle size and shape evolution. The high intensity of depolarized signal and the high value of rotational diffusion coefficient suggest that the aging process increases the thickness and the roughness of the nanoparticles.

An innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor.

An innovative chemical strategy integrated in a miniaturized electrochemical device was developed for sensitive detection of a pathogen genome (HBV virus) without any amplification step. The results show a limit of detection comparable to the standard qRT-PCR method (20 copies per reaction), paving the way to future development of genetic PoC devices addressing automatized and low-cost molecular diagnostics.

Optical trapping of silver nanoplatelets.

Optical trapping of silver nanoplatelets obtained with a simple room temperature chemical synthesis technique is reported. Trap spring constants are measured for platelets with different diameters to investigate the size-scaling behaviour. Experimental data are compared with models of optical forces based on the dipole approximation and on electromagnetic scattering within a T-matrix framework. Finally, we discuss applications of these nanoplatelets for surface-enhanced Raman spectroscopy.

Note: Dead time causes and correction method for single photon avalanche diode devices.

Single photon avalanche diode (SPAD) is the new generation of Geiger-Muller (GM) detectors, developed with semiconductor technology, and able to detect single photons, mainly in visible range. In this work we study the signal generation process and the dead time (DT) mechanisms of the device under a constant light regime. According to our results, it is possible to discriminate low rate signals from afterpulse and noise production and, moreover, to overcome the saturation effect due to the dead time losses. Starting from hybrid DT model [S. H. Lee and R. P. Gardner, Appl. Radiat. Isot. 53, 731 (2000)] we have been able to evaluate the real amount of incident photon rate up to 10(7) cps using a passive quenched device with 0.97 mus total dead time. In this way the passive quenched SPAD achieves the same performance of the active quenched one showing that relatively complex data analysis and complex device implementation are comparable solutions for constant light measurement. We also analyze some effects, lacking in GM counter, which should be introduced in the analysis of semiconductor device, as afterpulse, reduced photon detection efficiency, and noise production.