Breeding sites of Culicoides pachymerus Lutz in the Magdalena River basin, Colombia.

The breeding sites of Culicoides pachymerus are described for the first time in western Boyacá Province, Colombia, where this species is a public health problem. In addition to being a nuisance due to its enormous density and its high biting rates, C. pachymerus cause dermatological problems in the human population. Analysis of microhabitats by the sugar flotation technique and the use of emergence traps allowed us to recover 155 larvae of Culicoides spp and 65 adults of C. pachymerus from peridomiciliary muddy substrates formed by springs of water and constant rainwater accumulation. These important findings could aid in the design of integrated control measures against this pest.

[Preliminary evaluation of the Culicoides biting nuisance (Diptera: Ceratopogonidae) in the province of Boyacá, Colombia]. / Diagnóstico preliminar de la molestia sanitaria causada por Culicoides (Diptera: Ceratopogonidae) en el departamento de Boyacá, Colombia.

INTRODUCTION: Inhabitants in the western border of Boyacá province have reported high nuisance levels and dermatologic problems caused by the intensely irritating bites of the very small flies of the genus Culicoides. OBJECTIVE: A survey was carried out to locate the affected area, identify the anthropophylic Culicoides species and estimate its abundance in Boyacá. MATERIALS AND METHODS: Nuisance reports and clinical records of dermatologic cases associated with Culicoides bites were requested from health authorities in counties where nuisance reports had been received or which had geographical features apparently favorable for Culicoides infestations. An outdoors entomological survey using human landing catches was undertaken in areas reporting a pest problem. RESULTS: Culicoides infestations were confirmed as a serious nuisance problem in the rural areas of nine counties located in the western foothills of the Eastern Range of the Colombian Andes. Although available epidemiological records were fragmented, it was established that in six counties 11.4% of the dermatitis cases (total=2,472 cases) reported between 2003 and 2005 were attributed to the Culicoides bites. The entomological survey identified Culicoides pachymerus as the dominant species, 99.3% of 3,389 caught females. Biting rates in the most intensely affected areas reach a geometric mean of 52 females/person per 5 minutes. Multivariate analysis indicated that abundance of C. pachymerus had a negative relationship with altitude. CONCLUSIONS: Based on its dominance and high biting rates, C. pachymerus is probably the species responsible for the high nuisance levels caused by Culicoides bites and the associated dermatological pathology, within the study area.

[Presence of infected vectors of Leishmania (V.) panamensis within dwellings in two endemic foci in the foothill of the middle Magdalena valley, western Boyacá, Colombia]. / Presencia en el peridomicilio de vectores infectados con Leishmania (Viannia) panamensis en dos focos endémicos en el occidente de Boyacá, piedemonte del valle del Magdalena medio, Colombia.

INTRODUCTION: Case records of leishmaniasis of the years 1997 to 2003 of the department of Boyaca showed that since the year 2000 the department experienced an unusual rise in the incidence of cutaneous leishmaniasis that might correspond to an epidemic outbreak in the western region of the department. Age and gender distribution of cases supported a domestic transmission. OBJECTIVE: This research was designed to identify the vectors of cutaneous leishmaniasis in the municipalities of Otanche and Pauna through their presence in dwellings and their natural infection with the same species of Leishmania isolated from patients. MATERIAL AND METHODS: Sampling of sand flies was done with CDC traps in and around dwellings. Samples from patients and pooled females of the most abundant species of Lutzomyia were used to identify the parasite by PCR. Monoclonal antibody typing was also used to confirm the identification of the parasite in samples from patients. RESULTS: In both municipalities L. trapidoi was the most abundant anthropophilic species of Lutzomyia indoors and around dwellings. L. hartmanni and L. yuilli were also abundant in Otanche, and L. gomezi and L. panamensis in Pauna. Leishmania (V) panamensis was identified both in patients and in the sand flies: L. yuilli, L. gomezi and L. panamensis.. CONCLUSION: Our findings prove the presence of infected vectors of Leishmania panamensis within dwellings in the towns of Otanche and Pauna in Western Boyacá. Since L. trapidoi was the most abundant species, it may be considered as the principal vector of Leishmania (V.) panamensis. The evidence of transmission within human dwellings warrants vector control at least in this environment.

[Field efficacy of repellent formulation containing para-menthane-3,8-diol and lemongrass against Culicoides pachymerus (Diptera: Ceratopogonidae) in Colombia]. / Eficacia en campo de un repelente a base de para-mentano-3,8-diol y aceite de limonaria contra Culicoides pachymerus (Diptera: Ceratopogonidae) en Colombia.

INTRODUCTION: Culicoides pachymerus is a major pest species for the inhabitants of the western Boyacá province of Colombia. OBJECTIVE: The effect of a repellent lotion based on p-menthane-3,8-diol (16%) and lemongrass oil (2%) was evaluated against the bites of C. pachymerus. MATERIALS AND METHODS: The repellent lotion was compared simultaneously with a control (no treatment) by human landing catches of C. pachymerus on the forearms of paired volunteers situated near human dwellings. Protection percentage and protection time for 3 to 6 h after repellent application was calculated. The test was repeated ten times. RESULTS: Only two females of C. pachymerus were collected on arms with the repellent treatment. In contrast, the mean biting rate in the untreated control was 47.7 midges/person/10 min. Mean protection percentage of the repellent was 100% up to 4 h and 99.5% up to 5 h. Protection time was 332.2 and 338.2 min in the two replicates where bites of C. pachymerus were confirmed. In the remaining eight replicates protection time exceeded the test duration. CONCLUSION: The repellent showed high efficacy against C. pachymerus, up to 5 h post-application.

Breeding sites of Culicoides pachymerus Lutz in the Magdalena River basin, Colombia

The breeding sites of Culicoides pachymerus are described for the first time in western Boyacá Province, Colombia, where this species is a public health problem. In addition to being a nuisance due to its enormous density and its high biting rates, C. pachymerus cause dermatological problems in the human population. Analysis of microhabitats by the sugar flotation technique and the use of emergence traps allowed us to recover 155 larvae of Culicoides spp and 65 adults of C. pachymerus from peridomiciliary muddy substrates formed by springs of water and constant rainwater accumulation. These important findings could aid in the design of integrated control meas-ures against this pest.

Diagnóstico preliminar de la molestia sanitaria causada por Culicoides (Diptera: Ceratopogonidae) / Culicoides biting nuisance (Diptera: Ceratopogonidae) in the province of Boyacá, Colombia

Introducción. Los habitantes del occidente del departamento de Boyacá han reportado molestia sanitaria y problemas dermatológicos ocasionados por la constante picadura de insectos del género Culicoides. Objetivo. Identificar el área de Boyacá afectada por Culicoides, determinar las especies antropofílicas involucradas y su abundancia. Materiales y métodos. Se solicitó información sobre la molestia sanitaria y el registro decasos dermatológicos asociados a la picadura de Culicoides a las autoridades de salud de los municipios que por sus reportes previos o por sus características geográficas se consideraron como potencialmente afectados. En los municipios que informaron sufrir la problemática, se realizó un muestreo entomológico con atrayente humano afuera de las viviendas. Resultados. Se confirmó la gravedad de la molestia en el área rural de nueve municipios ubicados en el flanco occidental de la Cordillera Oriental. Aunque los registros epidemiológicos fueron fragmentados, se estableció que en seis municipios el 11,4 por ciento de los casos (n=2.472) dedermatitis reportados entre el 2003 y el 2005 fueron atribuidos a la picadura de Culicoides. Los resultados entomológicos mostraron que la especie dominante fue Culicoides pachymerus, 99,3 porcentaje de las 3.389 hembras recolectadas, con tasas de picadura (promedios geométricos) por municipio de hasta 52 hembras/persona en 5 minutos. Mediante análisis multivariado, se encontró que la abundancia de esta especie se relaciona negativamente con la altitud. Conclusiones. Por su dominancia y altas tasas de picadura, C. pachymerus es muy probablemente la especie responsable de la molestia sanitaria y los problemas dermatológicos causados por Culicoides en el departamento de Boyacá.

Presencia en el peridomicilio de vectores infectados con Leishmania (Viannia) panamensis en dos focos endémicos en el occidente de Boyacá, piedemonte del valle del Magdalena medio, Colombia / Presence of infected vectors of Leishmania (V. ) panamensis within dwellings in two endemic foci in the foothill of the middle Magdalena valley, western Boyacá, Colombia

Introducción. En el departamento de Boyacá, los casos de leishmaniasis registrados de 1997 a 2003 muestran un aumento en la incidencia a partir del año 2000 que puede corresponder a una epidemia en el occidente del departamento. Además, la distribución de casos por sexo y edad sugiere transmisión domiciliar. Objetivo. Definir los vectores de leishmaniasis cutánea en los municipios de Otanche y Pauna , por su asociación con el domicilio y por su infección natural con la misma especie de Leishmania aislada de pacientes. Materiales y métodos. Se recolectaron ejemplares de Lutzomyia con trampas CDC en el intra y peridomicilio. La identificación del parásito se hizo, mediante PCR, a partir de muestras de pacientes y en grupos de hembras de las especies de Lutzomyia más abundantes. Además se confirmó la especie de parásito en muestras de pacientes por anticuerpos monoclonales. Resultados. En los dos municipios, L. trapidoi fue la especie antropofílica mas abundante en y alrededor de las viviendas. L. hartmanni y L. yuilli fueron también abundantes en Otanche; y, L. gomezi y L. panamensis en Pauna . Leishmania (V.) panamensis se identificó tanto en pacientes como en los flebótomos: L. yuilli, L. gomezi y L. panamensis. Conclusión. Nuestros resultados confirman la presencia de vectores de Leishmania panamensis infectados naturalmente, en las viviendas de los municipios de Otanche y Pauna del Occidente de Boyacá. L. trapidoi por ser la especie más abundante puede ser considerada como el vector principal. La evidencia de transmisión doméstica permite planear la aplicación de medidas de control vectorial a este nivel.

Introduction. Case records of leishmaniasis of the years 1997 to 2003 of the department of Boyaca showed that since the year 2000 the department experienced an unusual rise in the incidence of cutaneous leishmaniasis that might correspond to an epidemic outbreak in the western region of the department. Age and gender distribution of cases supported a domestic transmission. Objective. This research was designed to identify the vectors of cutaneous leishmaniasis in the municipalities of Otanche and Pauna through their presence in dwellings and their natural infection with the same species of Leishmania isolated from patients. Material and methods. Sampling of sand flies was done with CDC traps in and around dwellings. Samples from patients and pooled females of the most abundant species of Lutzomyia were used to identify the parasite by PCR. Monoclonal antibody typing was also used to confirm the identification of the parasite in samples from patients. Results. In both municipalities L. trapidoi was the most abundant anthropophilic species of Lutzomyia indoors and around dwellings. L. hartmanni and L. yuilli were also abundant in Otanche, and L. gomezi and L. panamensis in Pauna . Leishmania (V) panamensis was identified both in patients and in the sand flies: L.yuilli, L. gomezi and L. panamensis.. Conclusion. Our findings prove the presence of infected vectors of Leishmania panamensis within dwellings in the towns of Otanche and Pauna in Western Boyacá. Since L. trapidoi was the most abundant species, it may be considered as the principal vector of Leishmania (V.) panamensis. The evidence of transmission within human dwellings warrants vector control at least in this environment.