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1.
Am J Physiol Regul Integr Comp Physiol ; 301(4): R1186-98, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21813869

RESUMO

Besides nitric oxide (NO) and carbon monoxide (CO), hydrogen sulfide (H(2)S) is a third gaseous messenger that may play a role in controlling vascular tone and has been proposed to serve as an O(2) sensor. However, whether H(2)S is vasoactive in the ductus arteriosus (DA) has not yet been studied. We investigated, using wire myography, the mechanical responses induced by Na(2)S (1 µM-1 mM), which forms H(2)S and HS(-) in solution, and by authentic CO (0.1 µM-0.1 mM) in DA rings from 19-day chicken embryos. Na(2)S elicited a 100% relaxation (pD(2) 4.02) of 21% O(2)-contracted and a 50.3% relaxation of 62.5 mM KCl-contracted DA rings. Na(2)S-induced relaxation was not affected by presence of the NO synthase inhibitor l-NAME, the soluble guanylate cyclase (sGC) inhibitor ODQ, or the K(+) channel inhibitors tetraethylammonium (TEA; nonselective), 4-aminopyridine (4-AP, K(V)), glibenclamide (K(ATP)), iberiotoxin (BK(Ca)), TRAM-34 (IK(Ca)), and apamin (SK(Ca)). CO also relaxed O(2)-contracted (60.8% relaxation) and KCl-contracted (18.6% relaxation) DA rings. CO-induced relaxation was impaired by ODQ, TEA, and 4-AP (but not by L-NAME, glibenclamide, iberiotoxin, TRAM-34 or apamin), suggesting the involvement of sGC and K(V) channel stimulation. The presence of inhibitors of H(2)S or CO synthesis as well as the H(2)S precursor L-cysteine or the CO precursor hemin did not significantly affect the response of the DA to changes in O(2) tension. Endothelium-dependent and -independent relaxations were also unaffected. In conclusion, our results indicate that the gasotransmitters H(2)S and CO are vasoactive in the chicken DA but they do not suggest an important role for endogenous H(2)S or CO in the control of chicken ductal reactivity.


Assuntos
Monóxido de Carbono/farmacologia , Embrião de Galinha/fisiologia , Canal Arterial/efeitos dos fármacos , Sulfeto de Hidrogênio/farmacologia , Vasodilatadores/farmacologia , 4-Aminopiridina/farmacologia , Animais , Monóxido de Carbono/antagonistas & inibidores , Monóxido de Carbono/metabolismo , Embrião de Galinha/efeitos dos fármacos , Canal Arterial/fisiologia , Glibureto/farmacologia , Sulfeto de Hidrogênio/antagonistas & inibidores , Sulfeto de Hidrogênio/metabolismo , Modelos Animais , NG-Nitroarginina Metil Éster/farmacologia , Oxigênio/farmacologia , Peptídeos/farmacologia , Sulfetos/farmacologia , Tetraetilamônio/farmacologia , Vasodilatação/efeitos dos fármacos , Vasodilatação/fisiologia
2.
Artigo em Inglês | MEDLINE | ID: mdl-21565280

RESUMO

Isoprostanes (IsoPs) are prostaglandin (PG)-like compounds produced nonenzymatically by free radical-catalyzed peroxidation of arachidonate. Cyclooxygenase-derived PGs play a major role in ductus arteriosus (DA) homeostasis but the putative role of IsoPs has not been studied so far. We investigated, using wire myography, the vasoactive effects of 15-E(2t)-IsoP and 15-F(2t)-IsoP in the chicken embryo DA, pulmonary artery (PA) and femoral artery (FA). 15-E(2t)-IsoP and 15-F(2t)-IsoP contracted DA, PA, and FA rings in a concentration-dependent manner. 15-E(2t)-IsoP was equally efficacious (mean±SE E(max)=1.25±0.06 mN/mm) as and more potent (-log of molar concentration producing 50% of E(max)=pEC(50)=7.00±0.04) than the thromboxane-prostanoid (TP) receptor agonist U46619 (E(max)=1.49±0.11 mN/mm; pEC(50)=6.48±0.05) in contracting chicken DA (pulmonary side). 15-F(2t)-IsoP was less potent (pEC(50)=5.74±0.11) and less efficacious (E(max)=0.96±0.11) than U46619. Concentration-dependent contractions to 15-E(2t)-IsoP and U46619 in DA rings were competitively inhibited by the TP receptor antagonist SQ29548 (0.1 µM to 10 µM) with no decrease in the E(max) values. SQ29548 also inhibited concentration-dependent contraction to 15-F(2t)-IsoP but this inhibition was associated with a decrease in E(max). Pre-incubation of DA rings with 15-F(2t)-IsoP inhibited responses to U46619 and, in vessels contracted with U46619 (1 µM), 15-F(2t)-IsoP (>1 µM) evoked a relaxant response. Enzyme immunoassay did not show a measurable release of 15-F(2t)-IsoP by DA rings. In conclusion, 15-E(2t)-IsoP is a potent and efficacious constrictor of chicken DA, acting through TP receptors. In contrast, 15-F(2t)-IsoP is probably acting as a partial agonist at TP receptors. We speculate that IsoPs play a role in the control of chicken DA tone and could participate in its closure.


Assuntos
Canal Arterial/embriologia , Artéria Femoral/efeitos dos fármacos , Isoprostanos/metabolismo , Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico/farmacologia , Animais , Embrião de Galinha , Dinoprosta/análogos & derivados , Canal Arterial/efeitos dos fármacos , Contração Muscular/fisiologia , Músculo Liso Vascular/fisiologia , Miografia/métodos , Estresse Oxidativo , Prostaglandinas/metabolismo , Artéria Pulmonar/efeitos dos fármacos , Receptores de Prostaglandina/agonistas , Receptores de Prostaglandina/antagonistas & inibidores , Receptores de Tromboxanos/agonistas , Receptores de Tromboxanos/antagonistas & inibidores , Vasoconstrição/fisiologia , Vasoconstritores/metabolismo
3.
Am J Physiol Regul Integr Comp Physiol ; 298(5): R1217-24, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20164203

RESUMO

Sex hormones have an important influence on cardiovascular physiology and pathophysiology and sex differences in vascular reactivity have been widely demonstrated. In the present study we hypothesized 1) the presence of sexual dimorphism in chicken ductus arteriosus (DA) responsiveness to contractile and relaxant stimuli and 2) that estrogens are vasoactive in the chicken DA. In vitro contractions (assessed with a wire myograph) induced by normoxia, KCl, 4-aminopyridine, norepinephrine, phenylephrine, U46619, or endothelin-1, as well as relaxations induced by ACh, sodium nitroprusside, BAY 41-2272, PGE(2), isoproterenol, forskolin,Y-27632, and hydroxyfasudil were not significantly different between males and females. The estrogen 17beta-estradiol elicited concentration-dependent relaxation of KCl-, phenylephrine-, and oxygen-induced active tone in male and female chicken DA. The stereoisomer 17alpha-estradiol showed lesser relaxant effects, and the selective estrogen receptor (ER) agonists 4,4',4''-(4-propyl-[(1)H]pyrazole-1,3,5-triyl)tris-phenol (ERalpha) and 2,3-bis(4-hydroxyphenyl)-propionitrile (ERbeta) did not show any effect. There were no sex differences in the responses to estrogen. Endothelium removal or the presence of the soluble guanylate cyclase inhibitor ODQ, the K(+) channel blockers tetraethylammonium, glibenclamide, and charybdotoxin, or the ER antagonist fulvestrant did not modify 17beta-estradiol-induced relaxation. CaCl(2) (30 muM-10 mM) induced concentration-dependent contraction in DA rings depolarized by 62.5 mM KCl or stimulated with 21% O(2) in Ca(2+)-free medium. Preincubation with 17beta-estradiol or the L-type Ca(2+) channel blocker nifedipine produced an inhibition of CaCl(2)-induced contractions. In conclusion, there are no sex-related differences in chicken DA reactivity. The estrogen 17beta-estradiol induces an endothelium-independent relaxation of chicken DA that is not mediated by ER activation. This relaxant effect is, at least partially, due to inhibition of Ca(2+) entry from extracellular space.


Assuntos
Canal Arterial , Estradiol/metabolismo , Estradiol/farmacologia , Caracteres Sexuais , Vasoconstrição/efeitos dos fármacos , Vasoconstrição/fisiologia , Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico/farmacologia , 4-Aminopiridina/farmacologia , Acetilcolina , Animais , Cálcio/metabolismo , Embrião de Galinha , Galinhas , Colforsina/farmacologia , Dinoprostona/farmacologia , Canal Arterial/efeitos dos fármacos , Canal Arterial/embriologia , Canal Arterial/fisiologia , Feminino , Masculino , Nitroprussiato/farmacologia , Norepinefrina/farmacologia , Oxigênio/metabolismo , Oxigênio/farmacologia , Bloqueadores dos Canais de Potássio/farmacologia , Cloreto de Potássio/farmacologia , Pirazóis/farmacologia , Piridinas/farmacologia , Receptores de Estrogênio/agonistas , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Vasoconstritores/farmacologia , Vasodilatação/efeitos dos fármacos , Vasodilatação/fisiologia
4.
Am J Physiol Lung Cell Mol Physiol ; 297(4): L619-30, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19617310

RESUMO

The increase in O(2) tension after birth is a major factor stimulating ductus arteriosus (DA) constriction and closure. Here we studied the role of the mitochondrial electron transport chain (ETC) as sensor, H(2)O(2) as mediator, and voltage-gated potassium (K(V)) channels and Rho kinase as effectors of O(2)-induced contraction in the chicken DA during fetal development. Switching from 0% to 21% O(2) contracted the pulmonary side of the mature DA (mature pDA) but had no effect in immature pDA and relaxed the aortic side of the mature DA (mature aDA). This contraction of the pDA was attenuated by inhibitors of the mitochondrial ETC and by the H(2)O(2) scavenger polyethylene glycol (PEG)-catalase. Moreover, O(2) increased reactive oxygen species (ROS) production, measured with the fluorescent probes dihydroethidium and 2',7'-dichlorofluorescein, only in mature pDA. The H(2)O(2) analog t-butyl-hydroperoxide mimicked the responses to O(2) in the three vessels. In contrast to immature pDA cells, mature pDA cells exhibited high-amplitude O(2)-sensitive potassium currents. The K(V) channel blocker 4-aminopyridine prevented the current inhibition elicited by O(2). The L-type Ca(2+) (Ca(L)) channel blocker nifedipine and the Rho kinase inhibitors Y-27632 and hydroxyfasudil induced a similar relaxation when mature pDA were stimulated with O(2) or H(2)O(2). Moreover, the sensitivity to these drugs increased with maturation. Our results indicate the presence of a common mechanism for O(2) sensing/signaling in mammalian and nonmammalian DA and favor the idea that, rather than a single mechanism, a parallel maturation of the sensor and effectors is critical for O(2) sensitivity appearance during development.


Assuntos
Canais de Cálcio Tipo L/metabolismo , Canal Arterial/metabolismo , Complexo III da Cadeia de Transporte de Elétrons/metabolismo , Oxigênio/metabolismo , Canais de Potássio de Abertura Dependente da Tensão da Membrana/metabolismo , Quinases Associadas a rho/metabolismo , Animais , Aorta/efeitos dos fármacos , Aorta/metabolismo , Western Blotting , Embrião de Galinha , Complexo III da Cadeia de Transporte de Elétrons/antagonistas & inibidores , Eletrofisiologia , Peróxido de Hidrogênio/toxicidade , Oxidantes/farmacologia , Canais de Potássio de Abertura Dependente da Tensão da Membrana/antagonistas & inibidores , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Quinases Associadas a rho/antagonistas & inibidores
5.
Pediatr Res ; 65(3): 279-84, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19033883

RESUMO

The hypoxic conditions in which children with intrauterine growth retardation (IUGR) develop are hypothesized to alter the development of the ductus arteriosus (DA). We aimed to evaluate the effects of in ovo hypoxia on chicken DA morphometry and reactivity. Hypoxia (15% O2 from day 6 to 19 of the 21-d incubation period) produced a reduction in the body mass of the 19-d fetuses and a shortening of right and left DAs. However, ductal lumen and media cross-sectional areas were not affected by hypoxia. The ductal contractions induced by oxygen, KCl, H2O2, 4-aminopyridine, and endothelin-1 were similar in control and hypoxic fetuses. In contrast, the DAs from the hypoxic fetuses showed increased contractile responses to norepinephrine and phenylephrine and impaired relaxations to acetylcholine, sodium nitroprusside, and isoproterenol. The relaxations induced by 8-Br-cGMP, forskolin, Y-27632, and hydroxyfasudil were not altered by chronic hypoxia. In conclusion, chronic in ovo hypoxia-induced growth retardation in fetal chickens and altered the response of the DA to adrenergic agonists and to endothelium-dependent and -independent relaxing agents. Our observations support the concept that prolonged patency of the DA in infants with IUGR may be partially related with hypoxia-induced changes in local vascular mechanisms.


Assuntos
Canal Arterial/metabolismo , Canal Arterial/patologia , Retardo do Crescimento Fetal/patologia , Hipóxia/complicações , Agonistas Adrenérgicos/farmacologia , Animais , Galinhas , Canal Arterial/efeitos dos fármacos , Retardo do Crescimento Fetal/etiologia , Análise de Regressão , Vasodilatadores/farmacologia
6.
Respir Physiol Neurobiol ; 178(1): 66-74, 2011 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-21513818

RESUMO

During amniotic vertebrate development, the embryo and fetus employ a number of cardiovascular shunts. These shunts provide a right-to-left shunt of blood and are essential components of embryonic life ensuring proper blood circulation to developing organs and fetal gas exchanger, as well as bypassing the pulmonary circuit and the unventilated, fluid filled lungs. In this review we examine and compare the embryonic shunts available for fetal mammals and embryonic reptiles, including lizards, crocodilians, and birds. These groups have either a single ductus arteriosus (mammals) or paired ductus arteriosi that provide a right-to-left shunt of right ventricular output away from the unventilated lungs. The mammalian foramen ovale and the avian atrial foramina function as a right-to-left shunt of blood between the atria. The presence of atrial shunts in non-avian reptiles is unknown. Mammals have a venous shunt, the ductus venosus that diverts umbilical venous return away from the liver and towards the inferior vena cava and foramen ovale. Reptiles do not have a ductus venosus during the latter two thirds of development. While the fetal shunts are well characterized in numerous mammalian species, much less is known about the developmental physiology of the reptilian embryonic shunts. In the last years, the reactivity and the process of closure of the ductus arteriosus have been characterized in the chicken and the emu. In contrast, much less is known about embryonic shunts in the non-avian reptiles. It is possible that the single ventricle found in lizards, snakes, and turtles and the origin of the left aorta in the crocodilians play a significant role in the right-to-left embryonic shunt in these species.


Assuntos
Sistema Cardiovascular/embriologia , Coração Fetal/embriologia , Vertebrados/embriologia , Animais , Feminino , Coração Fetal/fisiologia , Feto/irrigação sanguínea , Feto/fisiologia , Humanos , Gravidez
7.
J Comp Physiol B ; 179(2): 133-43, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18726601

RESUMO

Prostaglandin E(2) (PGE(2)) is the major vasodilator prostanoid of the mammalian ductus arteriosus (DA). In the present study we analyzed the response of isolated DA rings from 15-, 19- and 21-day-old chicken embryos to PGE(2) and other vascular smooth muscle relaxing agents acting through the cyclic AMP signaling pathway. PGE(2) exhibited a relaxant response in the 15-day DA, but not in the 19- and 21-day DA. Moreover, high concentrations of PGE(2) (>or= 3 microM in 15-day and >or= 1 microM in 19-day and 21-day DA) induced contraction of the chicken DA. The presence of the TP receptor antagonist SQ29,548, unmasked a relaxant effect of PGE(2) in the 19- and 21-day DA and increased the relaxation induced by PGE(2) in the 15-day DA. The presence of the EP receptor antagonist AH6809 abolished PGE(2)-mediated relaxation. The relaxant responses induced by PGE(2) and the beta-adrenoceptor agonist isoproterenol, but not those elicited by the adenylate cyclase activator forskolin or the phosphodiesterase 3 inhibitor milrinone, decreased with maturation. High oxygen concentrations (95%) decreased the relaxation to PGE(2). The relaxing potency and efficacy of isoproterenol and milrinone were higher in the pulmonary than in the aortic side of the DA, whereas no regional differences were found in the response to PGE(2). We conclude that, in contrast to the mammalian situation, PGE(2) is a weak relaxant agent of the chicken DA and, with advancing incubation, it even stimulates TP vasoconstrictive receptors.


Assuntos
Dinoprostona/farmacologia , Canal Arterial/efeitos dos fármacos , Fatores Etários , Análise de Variância , Animais , Compostos Bicíclicos Heterocíclicos com Pontes , Embrião de Galinha , AMP Cíclico/metabolismo , Dinoprostona/metabolismo , Ácidos Graxos Insaturados , Hidrazinas/farmacologia , Isoproterenol/farmacologia , Milrinona/farmacologia , Contração Muscular/efeitos dos fármacos , Xantonas/farmacologia
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