Preliminary assessment of environmental safety (ecosafety) of dextrin-based nanosponges for environmental applications.

The ability to employ waste products, such as vegetable scraps, as raw materials for the synthesis of new promising adsorbing materials is at the base of the circular economy and end of waste concepts. Dextrin-based nanosponges (D_NS), both cyclodextrin (CD) and maltodextrin (MD), have shown remarkable adsorption abilities in the removal of toxic compounds from water and wastewater, thus representing a bio-based low-cost solution which is establishing itself in the market. Nevertheless, their environmental safety for either aquatic or terrestrial organisms has been overlooked, raising concern in terms of potential hazards to natural ecosystems. Here, the environmental safety (ecosafety) of six newly synthesized batches of D_NS was determined along with their full characterization by means of dynamic light scattering (DLS), thermogravimetric analysis (TGA), Fourier transformed infrared spectroscopy with attenuated total reflection (FTIR-ATR) and transmission electron microscopy (SEM). Ecotoxicity evaluation was performed using a battery of model organisms and ecotoxicity assays, such as the microalgae growth inhibition test using the freshwater Raphidocelis subcapitata and the marine diatom Dunaliella tertiolecta, regeneration assay using the freshwater cnidarian Hydra vulgaris and immobilization assay with the marine brine shrimp Artemia franciscana. Impact on seedling germination of a terrestrial plant of commercial interest, Cucurbita pepo was also investigated. Ecotoxicity data showed mild to low toxicity of the six batches, up to 1 mg/mL, in the following order: R. subcapitata > H. vulgaris > D. tertiolecta > A. franciscana > C. pepo. The only exception was represented by one batch (NS-Q+_BDE_(GLU2) which resulted highly toxic for both freshwater species, R. subcapitata and H. vulgaris. Those criticalities were solved with the synthesis of a fresh new batch and were hence attributed to the single synthesis and not to the specific D_NS formulation. No effect on germination of pumpkin but rather more a stimulative effect was observed. To our knowledge this is the first evaluation of the environmental safety of D_ NS. As such we emphasize that current formulations and exposure levels in the range of mg/mL do not harm aquatic and terrestrial species thus representing an ecosafe solution also for environmental applications.

Improving wheat physio-biochemical attributes in ciprofloxacin-polluted saline soil using nZVI-modified biochar.

The Ciprofloxacin (CIP) toxicity and salinity stress in agricultural soils cause risk to environmental and food safety. Consequently, it is essential to devise or use more effective techniques for mitigating salinity and ciprofloxacin-induced stress in soil. This study includes the nZVI-loaded biochar synthesis, integrating the unique characteristics of raw biochar with nZVI. The present study examined the impact of raw and nZVI-loaded biochar on soil quality and the mitigation of salinity stress and Ciprofloxacin toxicity in wheat plants. The results showed that the application of nZVI-loaded biochar treatments led to substantial enhancement in shoot biomass, root biomass, grain biomass, and spike biomass by 152.1, 54.3 %, 59.8 %, and 151 %, respectively compared to control treatment. The treatment with nZVI-loaded biochar significantly increased the rates of photosynthesis and transpiration, as well as the conductance of stomata. It also resulted in higher levels of intercellular CO2, photosynthetic pigments, and water use efficiency with increases of 49 %, 59 %, 57 %, 37 %, 40 %, and 95 %, respectively. The nZVI-loaded biochar significantly decreased electrolyte leakage, malondialdehyde (MDA), and hydrogen peroxide levels compared to the NaCl treatment alone. It also enhanced the activities of enzymatic antioxidants such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX). The non-enzymatic antioxidants including total soluble sugars (TSS), flavonoids, total soluble proteins (TSP), phenolics, ascorbic acid, anthocyanin, proline, and glycine betaine significantly influenced by the nZVI-loaded biochar. The nZVI-loaded biochar effectively alleviates the stress of soils that are contaminated with hazardous amounts of Ciprofloxacin while improving the soil and plant health.

Interconnected environmental challenges: heavy metal-drug interactions and their impacts on ecosystems.

Industrial expansion and inadequate environmental safety measures are major contributors to environmental contamination, with heavy metals (HMs) and pharmaceutical waste playing crucial roles. Their negative effects are most noticeable in aquatic species and vegetation, where they accumulate in tissues and cause harmful results. Interactions between HMs and pharmaceutical molecules result in the production of metal-drug complexes (MDCs), which have the potential to disturb diverse ecosystems and their interdependence. However, present studies frequently focus on individual pollutants and their effects on specific environmental parameters, leaving out the cumulative effects of pollutants and their processes across several environmental domains. To address this gap, this review emphasizes the environmental sources of HMs, elucidates their emission pathways during anthropogenic activities, investigates the interactions between HMs and pharmaceutical substances, and defines the mechanisms underlying the formation of MDCs across various ecosystems. Furthermore, this review underscores the simultaneous occurrence of HMs and pharmaceutical waste across diverse ecosystems, including the atmosphere, soil, and water resources, and their incorporation into biotic organisms across trophic levels. It is important to note that these complex compounds represent a higher risk than individual contaminants.

Potential Teratogenicity Effects of Metals on Avian Embryos.

Agricultural areas can provide sources of food and hiding and nesting places for wild birds. Thus, the chemical load of potentially toxic elements (Cd, Cu, Pb) due to industrial and agricultural activities can affect not only the adult birds but also the embryos developing in the egg. The toxic effects of heavy metals applied alone were investigated on chicken embryos in the early and late stages of embryonic development using injection and immersion treatment methods. On day 3 of incubation, permanent preparations were made from the embryos to study the early development stage. There were no significant differences observed in embryo deaths and developmental abnormalities in this stage. On day 19 of incubation, the number of embryonic deaths, the body weight of the embryos, and the type of developmental abnormalities were examined. The embryonic mortality was statistically higher in the groups treated with cadmium and lead in the case of the injection treatment. A significant increase in developmental disorders was observed in the copper-treated group using the immersion application. The body weight significantly decreased in the cadmium- and lead-treated group using both treatment methods. However, a significant change in the body weight in the copper-treated group was only realized due to the injection method.

Rethinking the Esterquats: Synthesis, Stability, Ecotoxicity and Applications of Esterquats Incorporating Analogs of Betaine or Choline as the Cation in Their Structure.

Esterquats constitute a unique group of quaternary ammonium salts (QASs) that contain an ester bond in the structure of the cation. Despite the numerous advantages of this class of compounds, only two mini-reviews discuss the subject of esterquats: the first one (2007) briefly summarizes their types, synthesis, and structural elements required for a beneficial environmental profile and only briefly covers their applications whereas the second one only reviews the stability of selected betaine-type esterquats in aqueous solutions. The rationale for writing this review is to critically reevaluate the relevant literature and provide others with a "state-of-the-art" snapshot of choline-type esterquats and betaine-type esterquats. Hence, the first part of this survey thoroughly summarizes the most important scientific reports demonstrating effective synthesis routes leading to the formation of both types of esterquats. In the second section, the susceptibility of esterquats to hydrolysis is explained, and the influence of various factors, such as the pH, the degree of salinity, or the temperature of the solution, was subjected to thorough analysis that includes quantitative components. The next two sections refer to various aspects associated with the ecotoxicity of esterquats. Consequently, their biodegradation and toxic effects on microorganisms are extensively analyzed as crucial factors that can affect their commercialization. Then, the reported applications of esterquats are briefly discussed, including the functionalization of macromolecules, such as cotton fabric as well as their successful utilization on a commercial scale. The last section demonstrates the most essential conclusions and reported drawbacks that allow us to elucidate future recommendations regarding the development of these promising chemicals.

Green Packaging Films with Antioxidant Activity Based on Pectin and Camellia sinensis Leaf Extract.

In the packaging materials sector, increasing globalization has created the need for increased efforts to develop consumer protection measures. Consequently, new packaging materials are being sought to replace petroleum-based materials in the future. For this reason, global awareness of the environmental problems associated with the use of synthetic and non-degradable packaging has increased the attention paid to bio-packaging based on natural and biodegradable polymers. The bio-packaging sector is developing innovations to address the sustainability issues facing the food packaging industry. Our research has shown that green matcha extract can be a promising source of antioxidants for the production of bioactive pectin films. This study further confirmed that green matcha extract can be a promising source of antioxidants for the production of bioactive pectin films. The antioxidant activity test showed high activity of films containing matcha extract. The antioxidant activity of films without matcha addition, P, PJ, PC, PJC, was negligible. The addition of matcha to the polymer matrix did not significantly affect the mechanical properties (TS, EB) of the films obtained. The addition of cellulose had the greatest effect on changing the mechanical properties. It caused a twofold increase in the mechanical properties of the obtained packaging films. The addition of matcha significantly improved the barrier properties (for PM films, the WVTR was 3.40 [g/m2d]; for PJM films the WVTR was 1.70 [g/m2d]). The green packaging films showed no toxic effects on the plants (Phacelia tanacetifolia, Salvia hispanica, Brassica napus) and invertebrates (Daphnia pulex, Chaoborus, Chironomus aprilinus) tested. The half-solubility time of the membranes in a solution mimicking gastric acid was also determined. The longest half-dissolution time of the films was about 2 min. Our research has therefore shown that the biodegradable and environmentally safe green packaging films with antioxidant activity that we have developed can be used as edible functional casings in the future, e.g., for sausages and other food products.

Recent Progress in Photoelectrochemical Sensing of Pesticides in Food and Environmental Samples: Photoactive Materials and Signaling Mechanisms.

Pesticides have become an integral part of modern agricultural practices, but their widespread use poses a significant threat to human health. As such, there is a pressing need to develop effective methods for detecting pesticides in food and environmental samples. Traditional chromatography methods and common rapid detection methods cannot satisfy accuracy, portability, long storage time, and solution stability at the same time. In recent years, photoelectrochemical (PEC) sensing technology has gained attention as a promising approach for detecting various pesticides due to its salient advantages, including high sensitivity, low cost, simple operation, fast response, and easy miniaturization, thus becoming a competitive candidate for real-time and on-site monitoring of pesticide levels. This review provides an overview of the recent advancements in PEC methods for pesticide detection and their applications in ensuring food and environmental safety, with a focus on the categories of photoactive materials, from single semiconductor to semiconductor-semiconductor heterojunction, and signaling mechanisms of PEC sensing platforms, including oxidation of pesticides, steric hindrance, generation/decrease in sacrificial agents, and introduction/release of photoactive materials. Additionally, this review will offer insights into future prospects and confrontations, thereby contributing novel perspectives to this evolving domain.

Research Progress on Detection of Pathogens in Medical Wastewater by Electrochemical Biosensors.

The detection of pathogens in medical wastewater is crucial due to the high content of pathogenic microorganisms that pose significant risks to public health and the environment. Medical wastewater, which includes waste from infectious disease and tuberculosis facilities, as well as comprehensive medical institutions, contains a variety of pathogens such as bacteria, viruses, fungi, and parasites. Traditional detection methods like nucleic acid detection and immunological assays, while effective, are often time-consuming, expensive, and not suitable for rapid detection in underdeveloped areas. Electrochemical biosensors offer a promising alternative with advantages including simplicity, rapid response, portability, and low cost. This paper reviews the sources of pathogens in medical wastewater, highlighting specific bacteria (e.g., E. coli, Salmonella, Staphylococcus aureus), viruses (e.g., enterovirus, respiratory viruses, hepatitis virus), parasites, and fungi. It also discusses various electrochemical biosensing techniques such as voltammetry, conductometry, impedance, photoelectrochemical, and electrochemiluminescent biosensors. These technologies facilitate the rapid, sensitive, and specific detection of pathogens, thereby supporting public health and environmental safety. Future research may should pay more attention on enhancing sensor sensitivity and specificity, developing portable and cost-effective devices, and innovating detection methods for diverse pathogens to improve public health protection and environmental monitoring.

Spatial distribution of Pb and Zn in soils under native vegetation in Southeast Brazil.

Heavy metals can play an important biological role as micronutrients but also as potentially toxic elements (PTEs). Understanding the natural concentrations of PTEs-Pb and Zn included-in soils allows for the identification and monitoring of contaminated areas and their role in environmental risk assessment. In this study, we aim to determine semi-total or natural and available concentrations of Pb and Zn in topsoils (0-20 cm depth) from 337 samples under native vegetation in the State of Minas Gerais, Brazil. Additionally, we sought to interpret the spatial geochemical variability using geostatistical techniques and quality reference values for these elements in soils were established. The semi-total concentrations were determined by flame and graphite furnace atomic absorption after microwave-assisted nitric acid digestion method. The available concentrations were extracted using the Mehlich-I extractor and determined by atomic absorption spectrometer. Spatial variability was modeled using semivariance estimators: Matheron's classic, Cressie and Hawkins' robust, and Cressie median estimators, the last two being less sensitive to extreme values. This allowed the construction of digital maps through kriging of semi-total Pb and Zn contents using the median estimator, as well as other soil properties by the robust estimator. The dominance of acidic pH and low CEC values reflects highly weathered low-fertility soils. Semi-total Pb contents ranged from 2.1 to 278 mg kg-1 (median: 9.35 mg kg-1) whereas semi-total Zn contents ranged from 2.7 to 495 mg kg-1 (median: 7.7 mg kg-1). The available Pb contents ranged from 0.1 to 6.92 mg kg-1 (median: 0.54 mg kg-1) whereas available Zn contents ranged from 0.1 to 78.2 mg kg-1 (median: 0.32 mg kg-1). The highest Pb and Zn concentrations were observed near Januária, in the northern part of the territory, probably on limestone rocks from the Bambuí group. Finally, the QRVs for Pb and Zn in natural soils were lower than their background values from other Brazilian region and below the prevention values suggested by Brazilian environmental regulations.

Host-mediated gene engineering and microbiome-based technology optimization for sustainable agriculture and environment.

The agricultural sector and environmental safety both work hand in hand to promote sustainability in important issues like soil health, plant nutrition, food safety, and security. The conventional methods have greatly harmed the environment and people's health and caused soil fertility and quality to decline as well as deteriorate. Keeping in view the excessive exploitation and cascade of degradation events due to unsustainable farming practices, the need of the hour demands choosing an appropriate, eco-friendly strategy to restore soil health, plant nutrition, and environmental aspects. The priority highlights a need for a sustainable and environment-friendly upgradation of the present agricultural systems to utilize the beneficial aspects related to harnessing the gene-microbiome strategies which would help in the restoration and replenishment of the microbial pool. Thus, exploring the microbiome is the utmost priority which gives a deep insight into the different aspects related to soil and plant and stands out as an important contributor to plant health and productivity. "Microbes" are important drivers for the biogeochemical cycles and targets like sustainability and safety. This essential microbial bulk (soil microbiome) is greatly influenced by agricultural/farming practices. Therefore, with the help of microbiome engineering technologies like meta-transcriptomics, meta-proteomics, metabolomics, and novel gene-altering techniques, we can easily screen out the highly diverse and balanced microbial population in the bulk of soil, enhancing the soil's health and productivity. Importantly, we need to change our cultivation strategies to attain such sustainability. There is an urgent need to revert to natural/organic systems of cultivation patterns where the microbiome hub can be properly utilized to strengthen soil health, decrease insect pest and disease incidence, reduce greenhouse gas emissions, and ultimately prevent environmental degradation. Through this article, we wish to propose a shift in the cultivation pattern from chemical to the novel, upgraded gene-assisted designed eco-friendly methodologies which can help in incorporating, exploring, and harnessing the right microbiome consortium and can further help in the progression of environmentally friendly microbiome technologies for agricultural safety and productivity.

Advances on removal of organophosphorus pesticides with electrochemical technology.

Widespread use of organophosphorus pesticides (OPs), especially superfluous and unreasonable use, had brought huge harm to the environment and food chain. It is because only a small part of the pesticides sprayed reached the target, and the rest slid across the soil, causing pollution of groundwater and surface water resources. These pesticides accumulate in the environment, causing environmental pollution. Therefore, in recent years, the control and degradation of OPs have become a public spotlight and research hotspot. Due to its unique advantages such as versatility, environmental compatibility, controllability, and cost-effectiveness compatibility, electrochemical technology has become one of the most promising methods for degradation of OPs. The fundamental knowledge about electrochemical degradation on OPs was introduced in this review. Then, a comprehensive overview of four main types of practical electrochemical technologies to degrade pesticides were presented and evaluated. The knowledge contained herein should conduce to better understand the degradation of pesticides by electrochemical technology, and better exploit the degradation of pesticides in the environment and food. Overall, the objective of this review is to provide comprehensive guidance for rational design and application of electrochemical technology in the degradation of OPs for the safety of the environment and food chain in the future.

Metagenomics reveals the self-recovery and risk of antibiotic resistomes during carcass decomposition of wild mammals.

Animal carcass decomposition may bring serious harm to the environment, including pathogenic viruses, toxic gases and metabolites, and antibiotic resistance genes (ARGs). However, how wild mammal corpses decomposition influence and change ARGs in the environment has less explored. Through metagenomics, 16S rRNA gene sequencing, and physicochemical analysis, this study explored the succession patterns, influencing factors, and assembly process of ARGs and mobile genetic elements (MGEs) in gravesoil during long-term corpse decomposition of wild mammals. Our results indicate that the ARG and MGE communities related to wildlife corpses exhibited a pattern of differentiation first and then convergence. Different from the farmed animals, the decomposition of wild animals first reduced the diversity of ARGs and MGEs, and then recovered to a level similar to that of the control group (untreated soil). ARGs and MGEs of the gravesoil are mainly affected by deterministic processes in different stages. MGEs and bacterial community are the two most important factors affecting ARGs in gravesoil. It is worth noting that the decomposition of wild animal carcasses enriched different high-risk ARGs at different stages (bacA, mecA and floR), which have co-occurrence patterns with opportunistic pathogens (Comamonas and Acinetobacter), thereby posing a great threat to public health. These results are of great significance for wildlife corpse management and environmental and ecological safety.

Stability and safety key factors of the oncolytic protoparvovirus H-1 from manufacturing to human application.

The oncolytic rodent protoparvovirus H-1PV has been successfully used in phase I/II clinical trials to treat recurrent glioblastoma multiforme and pancreatic cancer. The present work focuses on the stability and environmental safety of the H-1PV drug product from production up to its use in patients. We identified hold-steps in manufacturing for up to 3 months and showed 7-years stability for the optimal product formulation. Stress testing via UV, temperature, and pH also determined that the drug product is stable. De- and rehydration for lyophilization simulation are possible without infectious virus loss. Furthermore, we prove in-use stability for 4 days at room temperature and show no virus adsorption to injection devices, guaranteeing the correct administration dose. Iodixanol in the formulation, resulting in high viscosity, protects H-1PV against UV and some disinfectants. Nonetheless, H-1PV is depleted with rapid heat deactivation, autoclavation, and nanofiltration. Assessment of chemical disinfectants that are currently recommended by the Robert Koch-Institute demonstrated that ethanol-based hand disinfectants are not effective; however, aldehyde-based disinfectants for surfaces and instruments demonstrate sufficient H-1PV deactivation in aqueous formulations by 4 to 6 log10. With these results, we could establish a specific hygiene plan for all involved facilities from manufacturing to patient application. Overall, using 48% Iodixanol in Visipaque/Ringer as a drug formulation stabilizes H-1PV infectivity over years and protects against virus loss from short-term UV, low pH, and temperature exposure. KEY POINTS: • Optimal formulation of drug product protects the H-1PV protoparvovirus against UV, temperatures up to 50 °C, and low pH (> 1.25), stabilizing the virus during manufacturing, storage, transport, and application. • H-1PV is stable during in-use and does not adsorb to injection devices during patient administration. • Hygiene plan for H-1PV with physicochemical methods has been established.

Factors affecting home environmental safety management for fall prevention for older adults in northern Thailand.

BACKGROUND: Falls among older adults affect physical and mental health, disability, death, and quality of life. Home environmental safety management helps to reduce the risk of falls among older adults. This analytical cross-sectional study aimed to determine factors affecting home environmental safety management for fall prevention for older adults in northern Thailand. METHODS: The study sample included 328 relatives who looked after older adults in their families in Phitsanulok Province, northern Thailand. They were randomly selected by a multistage sampling technique. Data were collected by a self-administered questionnaire consisting of 7 parts: (1) sociodemographic characteristics, (2) knowledge about home environmental safety management, (3) perceived susceptibility of falls, (4) perceived severity of falls among older adults, (5) perceived self-efficacy of home environmental safety management, (6) perceived outcome of home environmental safety management, and (7) home environmental safety management. Frequencies, percentages, means, standard deviations, and multiple regression analysis were employed for data analysis. RESULTS: The majority of participants (60.4%) had high scores for home environmental safety management for fall prevention for older adults (scores of 14-20). Factors that significantly affected home environmental safety management included perceived severity of falls among older adults (ß = 0.323), perceived self-efficacy of home environmental safety management (ß = 0.311), the elderly family member having fallen in the past year (ß = 0.217), being a grandchild of an older adult (ß = -0.143), perceived outcome of home environmental safety management (ß = 0.142), and being widowed, divorced or separated (ß = -0.096). These 6 factors explained 35.1% of home environmental safety management for fall prevention for older adults. CONCLUSION: Relatives who look after older adults should be educated about the perceived severity of falls among older adults, perceived self-efficacy, and perceived outcome of home environmental safety management. The focus should be on grandchildren of older adults and those who are widowed, divorced or separated to understand how home environmental safety management is important to prevent falls and their consequences among older adults.

Effects of copper oxide nanoparticles on reproductive system of zebrafish.

Copper oxide nanoparticles (CuO NPs) were regarded as the versatile materials in daily life and the in-depth evaluation of their biological effects is of great concern. Herein the female and male zebrafishes were chosen as the model animals to analyze the reproductive toxicity caused by CuO NPs at low concentration (10, 50 and 100 µg/L) After 20-days exposure, the structure of zebrafish ovary and testis were impaired. Moreover, the contents of 17ß-estradiol (E2) in both females and males were increased, while the contents of testosterone (T) were decreased, indicating the imbalanced sex hormones caused by CuO NPs. The expression of genes along the hypothalamic pituitary-gonad (HPG) axis, were examined with quantitative real-time PCR to further evaluate the toxic mechanisms. Meanwhile, the levels of erα/er2ß and cyp19a in female zebrafishes and erα/er2ß, lhr, hmgra/hmgrb, 3ßhsd and 17ßhsd in male zebrafishes were obviously up-regulated. While, the level of αr was obviously down-regulated in female and male zebrafishes. Thus, the obtained data uncovered that long-term exposure of CuO NPs with low dose could trigger the endocrine disorder, resulting in the disturbance of E2 and T level, inhibition of gonad development, and alteration of HPG axis genes. In brief, this study enriched the toxicological data of NPs on aquatic vertebrates and provided the theoretical support for assessing the environmental safety of NPs.

Predicting pesticide residues in pod fruits with a modified peel-like uptake model: A green pea demonstration.

Peas are among the most popular leguminous plants, consumed by both humans and animals in large quantities. Pesticides are widely used globally to increase pea yield, and as a result, pesticide residues can be taken up by pea plants and bioaccumulate in their fruits, including peas and pods. However, there is a lack of modeling approaches available to predict residue concentrations in peas. To address this issue, a pod fruit model (specifically designed for neutral organic compounds) was proposed to simulate the bioaccumulation process of pesticide residues in pea plants, which was developed by modifying a peel-like uptake model. The simulation results, based on green pea as the modeling demonstration, reveal that moderately-lipophilic pesticides (i.e., log KOW around 3) have higher simulated concentrations in peas at harvest compared to hydrophilic (i.e., log KOW less than 0) or highly-lipophilic (i.e., log KOW over 5) pesticides, which is due to the enhanced uptake process of moderately-lipophilic compounds in the pod-pea system, such as their ability to penetrate the pod cuticle and be transported via phloem sap. The sensitivity test and variability analysis conducted in this study revealed that the degradation kinetics, including metabolism, hydrolysis, and photolysis, had a significant impact on moderately-lipophilic pesticides due to their high simulated concentrations in the pea plant. This can result in substantial loss of residue mass via degradation. The validation of the model demonstrated that the simulation results, specifically residue concentrations in the fruit, were consistent with the harvested data. However, some inconsistency was observed immediately after pesticide application, which could be attributed to plant growth dynamics and initial surface mass distributions. The proposed pod fruit model provides new insights into the bioaccumulation process of pesticide residues in pea plants and enables high-throughput simulations of residue concentrations at harvest. To enhance the performance of the pod fruit model, future research should consider plant growth dynamics, plant uptake of ionizable compounds, and initial mass distribution functions.

A review of microplastic pollution in aquaculture: Sources, effects, removal strategies and prospects.

As microplastic pollution has become an emerging environmental issue of global concern, microplastics in aquaculture have become a research hotspot. For environmental safety, economic efficiency and food safety considerations, a comprehensive understanding of microplastic pollution in aquaculture is necessary. This review outlines an overview of sources and effects of microplastics in aquaculture. External environmental inputs and aquaculture processes are sources of microplastics in aquaculture. Microplastics may release harmful additives and adsorb pollutants in aquaculture environment, cause deterioration of aquaculture environment, as well as cause toxicological effects, affect the behavior, growth and reproduction of aquaculture products, ultimately reducing the economic benefits of aquaculture. Microplastics entering the human body through aquaculture products also pose potential health risks at multiple levels. Microplastic pollution removal strategies used in aquaculture in various countries are also reviewed. Ecological interception and purification are considered to be effective methods. In addition, strengthening aquaculture management and improving fishing gear and packaging are also currently feasible solutions. As proactive measures, new portable microplastic monitoring system and remote sensing technology are considered to have broad application prospects. And it was encouraged to comprehensively strengthen the supervision of microplastic pollution in aquaculture through talent exchange and strengthening the construction of laws and regulations.

Ionizing radiation affects the demography and the evolution of Caenorhabditis elegans populations.

Ionizing radiation can reduce survival, reproduction and affect development, and lead to the extinction of populations if their evolutionary response is insufficient. However, demographic and evolutionary studies on the effects of ionizing radiation are still scarce. Using an experimental evolution approach, we analyzed population growth rate and associated change in life history traits across generations in Caenorhabditis elegans populations exposed to 0, 1.4, and 50.0 mGy.h-1 of ionizing radiation (gamma external irradiation). We found a higher population growth rate in the 1.4 mGy.h-1 treatment and a lower in the 50.0 mGy.h-1 treatment compared to the control. Realized fecundity was lower in both 1.4 and 50.0 mGy.h-1 than control treatment. High irradiation levels decreased brood size from self-fertilized hermaphrodites, specifically early brood size. Finally, high irradiation levels decreased hatching success compared to the control condition. In reciprocal-transplant experiments, we found that life in low irradiation conditions led to the evolution of higher hatching success and late brood size. These changes could provide better tolerance against ionizing radiation, investing more in self-maintenance than in reproduction. These evolutionary changes were with some costs of adaptation. This study shows that ionizing radiation has both demographic and evolutionary consequences on populations.

Characterisation and toxicological activity of three different Pseudo-nitzschia species from the northern Adriatic Sea (Croatia).

Diatoms of the genus Pseudo-nitzschia are cosmopolitans spread in seas and oceans worldwide, with more than 50 described species, dozens of which have been confirmed to produce domoic acid (DA). Here, we characterized and investigated the toxicological activity of secondary metabolites excreted into the growth media of different Pseudo-nitzschia species sampled at various locations in the northern Adriatic Sea (Croatia) using human blood cells under in vitro conditions. The results revealed that three investigated species of the genus Pseudo-nitzschia were capable of producing DA indicating their toxic potential. Moreover, toxicological data suggested all three Pseudo-nitzschia species can excrete toxic secondary metabolites into the surrounding media in addition to the intracellular pools of DA, raising concerns regarding their toxicity and environmental impact. In addition, all three Pseudo-nitzchia species triggered oxidative stress, one of the mechanisms of action likely responsible for the DNA damage observed in human blood cells. In line with the above stated, our results are of great interest to environmental toxicologists, the public and policy makers, especially in light of today's climate change, which favours harmful algal blooms and the growth of DA producers with a presumed negative impact on the public health of coastal residents.

A critical review on the treatment of dye-containing wastewater: Ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety.

The synthetic dyes used in the textile industry pollute a large amount of water. Textile dyes do not bind tightly to the fabric and are discharged as effluent into the aquatic environment. As a result, the continuous discharge of wastewater from a large number of textile industries without prior treatment has significant negative consequences on the environment and human health. Textile dyes contaminate aquatic habitats and have the potential to be toxic to aquatic organisms, which may enter the food chain. This review will discuss the effects of textile dyes on water bodies, aquatic flora, and human health. Textile dyes degrade the esthetic quality of bodies of water by increasing biochemical and chemical oxygen demand, impairing photosynthesis, inhibiting plant growth, entering the food chain, providing recalcitrance and bioaccumulation, and potentially promoting toxicity, mutagenicity, and carcinogenicity. Therefore, dye-containing wastewater should be effectively treated using eco-friendly technologies to avoid negative effects on the environment, human health, and natural water resources. This review compares the most recent technologies which are commonly used to remove dye from textile wastewater, with a focus on the advantages and drawbacks of these various approaches. This review is expected to spark great interest among the research community who wish to combat the widespread risk of toxic organic pollutants generated by the textile industries.