RESUMO
A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium (THG-3.7T) was isolated from seawater. Growth occurred at 10-30 °C (optimum 25 °C), at pH 6-8 (optimum 7) and in the presence of 1-8â% (w/v) NaCl (optimum 4â%). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-3.7T were identified as Paraglaciecola mesophila DSM 15026T (95.3â% similarity), Glaciecola pallidula DSM 14239T (95.2â%), Paraglaciecola aquimarina KCTC 32108T (95.1â%), Paraglaciecola arctica KACC 14537T (94.9â%), Glaciecola nitratireducens KCTC 12276T (94.7â%) and Paraglaciecola psychrophila CGMCC 1.6130T (94.7â%). 16S rRNA gene sequence similarities among strain THG-3.7T and other species were lower than 94.7â%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The quinone system was composed of Q-8. The major fatty acids were C16â:â0, C18â:â1ω7c and summed feature 3 (C16â:â1ω7c and/or iso-C15â:â0 2-OH). The DNA G+C content of strain THG-3.7T was 47.9 mol%. On the basis of the data presented, strain THG-3.7T represents a novel species of the genus Glaciecola, for which the name Glaciecola amylolytica sp. nov. is proposed. The type strain is THG-3.7T (=KACC 19478T=CCTCC AB 2017258T).