Genotype-specific germination behavior induced by sustainable priming techniques in response to water deprivation stress in rice.

Water stress brought about by climate change is among the major global concerns threatening food security. Rice is an important staple food which requires high water resources. Being a semi-aquatic plant, rice is particularly susceptible to drought. The aim of this work was to develop techniques directed to promote rice resilience to water deprivation stress during germination by implementing specific seed priming treatments. Five popular Italian rice varieties were subjected to priming treatments using novel, sustainable solutions, like poly-gamma-glutamic acid (γ-PGA), denatured γ-PGA (dPGA), and iron (Fe) pulsing, alone or in combination. The effect of the developed priming methods was tested under optimal conditions as well as under water deprivation stress imposed by polyethylene glycol (PEG) treatments. The priming efficacy was phenotypically determined in terms of germination behavior by measuring a series of parameters (germinability, germination index, mean germination time, seed vigor index, root and shoot length, germination stress tolerance index). Biochemical analyses were carried out to measure the levels of iron uptake and accumulation of reactive oxygen species (ROS). Integrative data analyses revealed that the rice varieties exhibited a strong genotype- and treatment-specific germination behavior. PEG strongly inhibited germination while most of the priming treatments were able to rescue it in all varieties tested except for Unico, which can be defined as highly stress sensitive. Molecular events (DNA repair, antioxidant response, iron homeostasis) associated with the transition from seed to seedling were monitored in terms of changes in gene expression profiles in two varieties sensitive to water deprivation stress with different responses to priming. The investigated genes appeared to be differentially expressed in a genotype-, priming treatment-, stress- and stage-dependent manner. The proposed seed priming treatments can be envisioned as sustainable and versatile agricultural practices that could help in addressing the impact of climate challenges on the agri-food system.

Iron-pulsing, a novel seed invigoration technique to enhance crop yield in rice: A journey from lab to field aiming towards sustainable agriculture.

Bulk fertilizer application is one of the easiest means of improving yield of crops however it comes with several environmental impediments and consumer health menace. In the wake of this situation, sustainable agricultural practices stand as pertinent agronomic tool to increase yield and ensure sufficient food supply from farm to fork. In the present study, efficacy of iron-pulsing in improving the rice yield has been elucidated. This technique involves seed treatment with different concentrations (2.5, 5 and 10 mM) of iron salts (FeCl3 and FeSO4) during germination. FeCl3 or FeSO4 was used to treat the sets and depending on the concentration of the salts, the sets were named as C2.5, C5, C10 and S2.5, S5, S10 (where C and S stands for FeCl3 and FeSO4 respectively and the numbers succeeding them denotes the concentration of salt in mM). Our investigation identified 72 h of treatment as ideal duration for iron-pulsing. At this time point, the seedling emergence attributes and activities of α-amylase and protease increased. The relative water uptake of the seeds also increased through upregulation of aquaporin expression. The treatment efficiently maintained the ROS balance with the aid of antioxidant enzymes and increased the iron content within the treated seeds. After transplantation in field, photosynthetic rate and chlorophyll content enhanced in the treated plants. Finally, the post-harvest agro-morphological traits (represented through panicle morphology, 1000 seed weight, harvest index) and yield showed significant improvement with treatment. Sets C5 and S5 showed optimum efficiency in terms of yield improvement. To our best knowledge, this study is the first report deciphering the efficacy of iron-pulsing as a safe, cost effective and promising technique to escalate the yield of rice crops without incurring an environmental cost. Thus, iron-pulsing is expected to serve as a potential tool to address global food security in years to come.

Enhancement of nitrogen assimilation and photosynthetic efficiency by novel iron pulsing technique in Oryza sativa L. var Pankaj.

Rice is a major food crop. Due to urbanization and climate change, rice production is declining, posing a threat to the increasing food demand. For this, a modified technique of priming is used to enhance plant vigor. In the present study an endogenous rice cultivar was treated with two different iron salts for 72 h and grown for 14 days in nutrient solution. This increased the iron content of the samples which further escalated the photosynthetic efficiency and carbon assimilation in the treated plants. Photosynthesis being correlated to nitrogen assimilation, nitrogen assimilation intermediates and protein content were also elevated in treated plants. Plants showed no symptoms of stress as evident from low malondialdehyde content and increased antioxidant enzymes' activity. From this study it can be inferred that, treatment with iron during germination, helps to trigger growth by facilitating photosynthesis and nitrogen assimilation.