Phylogenomic reconstruction of Solenogastres (Mollusca, Aplacophora) informs hypotheses on body size evolution.

Body size is a fundamental characteristic of animals that impacts every aspect of their biology from anatomical complexity to ecology. In Mollusca, Solenogastres has been considered important to understanding the group's early evolution as most morphology-based phylogenetic reconstructions placed it as an early branching molluscan lineage. Under this scenario, molluscs were thought to have evolved from a small, turbellarian-like ancestor and small (i.e., macrofaunal) body size was inferred to be plesiomorphic for Solenogastres. More recently, phylogenomic studies have shown that aplacophorans (Solenogastres + Caudofoveata) form a clade with chitons (Polyplacophora), which is sister to all other molluscs, suggesting a relatively large-bodied (i.e., megafaunal) ancestor for Mollusca. Meanwhile, recent investigations into aplacophoran phylogeny have called the assumption that the last common ancestor of Solenogastres was small-bodied into question, but sampling of meiofaunal species was limited, biasing these studies towards large-bodied taxa and leaving fundamental questions about solenogaster body size evolution unanswered. Here, we supplemented available data with transcriptomes from eight diverse meiofaunal species of Solenogastres and conducted phylogenomic analyses on datasets of up to 949 genes. Maximum likelihood analyses support the meiofaunal family Meiomeniidae as the sister group to all other solenogasters, congruent with earlier ideas of a small-bodied ancestor of Solenogastres. In contrast, Bayesian Inference analyses support the large-bodied family Amphimeniidae as the sister group to all other solenogasters. Investigation of phylogenetic signal by comparing site-wise likelihood scores for the two competing hypotheses support the Meiomeniidae-first topology. In light of these results, we performed ancestral character state reconstruction to explore the implications of both hypotheses on understanding of Solenogaster evolution and review previous hypotheses about body size evolution and its potential consequences for solenogaster biology. Both hypotheses imply that body size evolution has been highly dynamic over the course of solenogaster evolution and that their relatively static body plan has successfully allowed for evolutionary transitions between meio-, macro- and megafaunal size ranges.

Rediscovering the unusual, solitary bryozoan Monobryozoon ambulans Remane, 1936: first molecular and new morphological data clarify its phylogenetic position.

BACKGROUND: One of the most peculiar groups of the mostly colonial phylum Bryozoa is the taxon Monobryozoon, whose name already implies non-colonial members of the phylum. Its peculiarity and highly unusual lifestyle as a meiobenthic clade living on sand grains has fascinated many biologists. In particular its systematic relationship to other bryozoans remains a mystery. Despite numerous searches for M. ambulans in its type locality Helgoland, a locality with a long-lasting marine station and tradition of numerous courses and workshops, it has never been reencountered until today. Here we report the first observations of this almost mythical species, Monobryozoon ambulans. RESULTS: For the first time since 1938, we present new modern, morphological analyses of this species as well as the first ever molecular data. Our detailed morphological analysis confirms most previous descriptions, but also ascertains the presence of special ambulatory polymorphic zooids. We consider these as bud anlagen that ultimately consecutively separate from the animal rendering it pseudo-colonial. The remaining morphological data show strong ties to alcyonidioidean ctenostome bryozoans. Our morphological data is in accordance with the phylogenomic analysis, which clusters it with species of Alcyonidium as a sister group to multiporate ctenostomes. Divergence time estimation and ancestral state reconstruction recover the solitary state of M. ambulans as a derived character that probably evolved in the Late Cretaceous. In this study, we also provide the entire mitogenome of M. ambulans, which-despite the momentary lack of comparable data-provides important data of a unique and rare species for comparative aspects in the future. CONCLUSIONS: We were able to provide first sequence data and modern morphological data for the unique bryozoan, M. ambulans, which are both supporting an alcyonidioidean relationship within ctenostome bryozoans.

Linkages between pelagic and benthic biota in a deteriorated coastal lake after restoration, Maruit, Egypt.

Until the 1960s, Lake Maruit was one of Egypt's most productive coastal brackish lakes. Continuous polluted discharge from Alexandria city resulted in long-term deterioration. The Egyptian government started a lake restoration program in 2010. Biological linkages between pelagic and benthic communities were assessed in November 2012 using parasitism and predation. This study examined ectoparasites infesting tilapia fish from 300 samples. The platyhelminth ectoparasite, Monogenea, and parasitic-copepod Ergasilus lizae were detected. Platyhelminthes parasitized Oreochromis niloticus and Oreochromis aureus, whereas the crustacean parasitized Coptodon zillii. The parasitic prevalence was low for Cichlidogyrus sp. and Ergasilus lizae. Benthic biotas were similar across basins. Fish abundance does not respond directly to benthic biotic components. Phytoplankton and benthic microalgae were not the main fish diet. Data on Halacaridae and fish clustered, indicating that either Halacaridae responds to their environment like fish or fish prey upon them because of their size. Linear correlations between pelagic, benthic biota, and parasite-infected fish indicate parasites may control their hosts. Some bioindicators indicate that stressed ecosystems differ from unstressed ecosystems. Fish species and biota abundances were low. Inconsistency in the food web and an absence of direct interactions between prey and predators are bioindicators of disturbed ecosystems. The low prevalence of ectoparasites and lack of heterogenous distribution of the various examined biota are bioindicators of habitat rehabilitation. Ongoing biomonitoring to better understand habitat rehabilitation is suggested.

Microbial communities in sandy beaches from the three domains of life differ by microhabitat and intertidal location.

The microbial communities of sandy beaches are poorly described despite the biogeochemical importance and ubiquity of these ecosystems. Using metabarcoding of the 16S and 18S rRNA genes, we investigated the diversity, microhabitats (with or between sand grains) and intertidal distributions of microorganisms (including meiofauna) from pristine sandy beaches in British Columbia, Canada, and hypothesized that abiotic variations due to microhabitat or intertidal gradients influence the distribution of microorganisms on local scales. Bacterial, archaeal and protistan communities of the sand were clearly distinct from interstitial communities, and from planktonic communities of the overlying seawater, which correlated with differences in function and lifestyle (e.g., sulphur reduction and gliding motility). In contrast, meiofaunal communities could not be distinguished by sample type, suggesting that they are more frequently mobilized between these microhabitats. Across intertidal zones, high intertidal, mid intertidal and low intertidal/swash communities were distinct and correlated with moisture, organic carbon and phosphate content, implying that the distribution of microorganisms is influenced by intertidal abiotic gradients. However, few taxa at the genus or species level individually contributed to this zonation pattern; rather, a unique combination of multiple microbial taxa was probably responsible. Although significant differences in microbial community composition on sandy beaches can be attributed to microhabitat and intertidal gradients, further investigations are needed to assess community assembly processes, the consistency of these distributions, and the functions of the majority of the microorganisms observed in the sand and their effects on the biogeochemistry and ecology of sandy beaches.

Phylogenomic analyses of mud dragons (Kinorhyncha).

Mud dragons (Kinorhyncha) are microscopic invertebrates, inhabiting marine sediments across the globe from intertidal to hadal depths. They are segmented, moulting animals like arthropods, but grouping with the unsegmented priapulans and loriciferans within Ecdysozoa. There are more than 300 species of kinorhynchs described within 31 genera and 11 families, however, their evolutionary relationships have so far only been investigated using morphology and a few molecular markers. Here we aim to resolve the relationships and classification of major clades within Kinorhyncha using transcriptomic data. In addition, we wish to revisit the position of three indistinctly segmented, aberrant genera in order to reconstruct the evolution of distinct segmentation within the group. We conducted a phylogenomic analysis of Kinorhyncha including 21 kinorhynch transcriptomes (of which 18 are new) representing 15 genera, and seven outgroups including priapulan, loriciferan, nematode and nematomorph transcriptomes. Results show a congruent and robust tree that supports the division of Kinorhyncha into two major clades: Cyclorhagida and Allomalorhagida. Cyclorhagida is composed of three subclades: Xenosomata, Kentrorhagata comb. nov. (including the aberrant Zelinkaderes) and Echinorhagata. Allomalorhagida is composed of two subclades: Pycnophyidae and Anomoirhaga nom. nov. Anomoirhaga nom. nov. accommodates the aberrant genera Cateria (previously nested within Cyclorhagida) and Franciscideres together with five additional genera. The distant and derived positions of the aberrant Zelinkaderes, Cateria and Franciscideres species suggest that their less distinct trunk segmentation evolved convergently, and that segmentation evolved among kinorhynch stem groups.

Three Free-Living Marine Nematodes from Sagami Bay, Japan, with a Description of Wiesoncholaimus jambio sp. nov. (Nematoda: Oncholaimidae).

We describe three free-living marine nematode species from Sagami Bay, Japan. Wiesoncholaimus jambiosp. nov. is the second species of this genus. It differs from the type species, Wiesoncholaimus mawsonae Inglis, 1966, by its dorsal tooth, which is as long as the other two teeth; its conico-cylindrical tail, which is as long as 4.6-4.8 cloacal body diameters; and the absence of the gubernaculum. Thalassironus cf. britannicus de Man, 1889 agrees well with a redescription of Thalassironus britannicus de Man, 1889 based on specimens collected near the type locality. However, as it is possible that T. britannicus sensu lato includes two or more species, our species may not be conspecific to T. britannicus sensu stricto. Vasostoma cf. longispicula Huang and Wu, 2010 closely resembles Vasostoma longispicula Huang and Wu, 2010 originally described from China, but minor differentiations are observed in body diameter, body cuticle thickness, and type of precloacal supplements.

Extreme freeze-tolerance in cryophilic tardigrades relies on controlled ice formation but does not involve significant change in transcription.

Subzero temperatures are among the most significant factors defining the distribution of organisms, yet, certain taxa have evolved to overcome this barrier. The microscopic tardigrades are among the most freeze-tolerant animals, with selected species reported to survive milli-Kelvin temperatures. Here, we estimate survival of fully hydrated eutardigrades of the species Ramazzottius varieornatus following exposures to -20 °C and  -80 °C as well as -196 °C with or without initial cooling to -80 °C. The tardigrades easily survive these temperatures, yet with a significant decrease in viability following rapid cooling by direct exposure to -196 °C. Hence, post-freeze recovery of R. varieornatus seems to rely on cooling rate and thus controlled ice formation. Cryophilic organisms are renowned for having cold-active enzymes that secure appropriate reaction rates at low temperatures. Hence, extreme freeze-tolerance in R. varieornatus could potentially involve syntheses of cryoprotectants and de novo transcription. We therefore generated a reference transcriptome for this cryophilic R. varieornatus population and explored for differential gene expression patterns following cooling to -80 °C as compared to active 5 °C controls. Specifically, we tested for fast transcription potentially occurring within 25 min of cooling from room temperature to a supercooling point of ca. -20 °C, at which the tardigrades presumably freeze and enter into the ametabolic state of cryobiosis. Our analyses revealed no evidence for differential gene expression. We, therefore, conclude that extreme freeze-tolerance in R. varieornatus relies on controlled extracellular freezing with any freeze-tolerance related genes being constitutively expressed.

Revisiting kinorhynch segmentation: variation of segmental patterns in the nervous system of three aberrant species.

BACKGROUND: Kinorhynch segmentation differs from the patterns found in Chordata, Arthropoda and Annelida which have coeloms and circulatory systems. Due to these differences and their obsolete status as 'Aschelminthes', the microscopic kinorhynchs are often not acknowledged as segmented bilaterians. Yet, morphological studies have shown a conserved segmental arrangement of ectodermal and mesodermal organ systems with spatial correspondence along the anterior-posterior axis. However, a few aberrant kinorhynch lineages present a worm-like body plan with thin cuticle and less distinct segmentation, and thus their study may aid to shed new light on the evolution of segmental patterns within Kinorhyncha. RESULTS: Here we found the nervous system in the aberrant Cateria styx and Franciscideres kalenesos to be clearly segmental, and similar to those of non-aberrant kinorhynchs; hereby not mirroring their otherwise aberrant and posteriorly shifted myoanatomy. In Zelinkaderes yong, however, the segmental arrangement of the nervous system is also shifted posteriorly and misaligned with respect to the cuticular segmentation. CONCLUSIONS: The morphological disparity together with the distant phylogenetic positions of F. kalenesos, C. styx and Z. yong support a convergent origin of aberrant appearances and segmental mismatches within Kinorhyncha.

Ecological quality assessement of marinas: An integrative approach combining biological and environmental data.

The importance of marinas as infrastructures for recreational boating is increasing substantially. However, information on their soft-bottom benthic communities, a key tool for managing programmes, is still scarce. We combined environment features with macro- and meiofaunal soft-bottom community information for assessing the ecological status of marinas with an integrative approach. To address this issue, we focused on eight marinas of the Southern Iberian Peninsula. Macro- and meiofauna data revealed high benthic heterogeneity at a spatial scale. The environmental variables which correlated best with macrofauna were mainly phosphorus, granulometry, and total organic carbon, and secondarily important variables were faecal coliforms, the biocide Irgarol, and heavy metals; total hydrocarbon concentration was also significant for meiofauna. Annelida was the dominant phylum in terms of number of species (37%) and abundance (66%) and were better descriptors of the environmental conditions than Arthropoda and Mollusca. Although identification to the species level is desirable and mandatory for assessing biological pollution, significant differences among marinas and correlations between fauna and abiotic variables were already detected at the level of family and order. This implies that biota assessment at higher levels may still be useful in monitoring programmes limited by time and budget constraints. The major novelty of this study lies in the development of an integrative assessment method based on the following selected ecological indicators: Marinas Environmental Pollution Index (MEPI), Biocontamination Index (BCI), macrofaunal biotic indices (AMBI, M-AMBI, BENTIX, MEDOCC and BENFES), macrofaunal taxa richness and Shannon-Wiener's diversity, and nematode:copepod index. This approach was able to discriminate marinas of the Southern Iberian Peninsula based on their ecological status, which ranged from poor to good. The method can be useful to design standards for assigning "sustainable quality seals" to those marinas with better values of ecological indicators.

Environmental DNA surveys detect distinct metazoan communities across abyssal plains and seamounts in the western Clarion Clipperton Zone.

The deep seafloor serves as a reservoir of biodiversity in the global ocean, with >80% of invertebrates at abyssal depths still undescribed. These diverse and remote deep-sea communities are critically under-sampled and increasingly threatened by anthropogenic impacts, including future polymetallic nodule mining. Using a multigene environmental DNA (eDNA) metabarcoding approach, we characterized metazoan communities sampled from sediments, polymetallic nodules and seawater in the western Clarion Clipperton Zone (CCZ) to test the hypotheses that deep seamounts (a) are species richness hotspots in the abyss, (b) have structurally distinct communities in comparison to other deep-sea habitats, and (c) that seafloor particulate organic carbon (POC) flux and polymetallic nodule density are positively correlated with metazoan diversity. eDNA metabarcoding was effective at characterizing distinct biotas known to occur in association with different abyssal substrate types (e.g., nodule- and sediment-specific fauna), with distinct community composition and few taxa shared across substrates. Seamount faunas had higher overall taxonomic richness, and different community composition and biogeography than adjacent abyssal plains, with seamount communities displaying less connectivity between regions than comparable assemblages on the abyssal plains. Across an estimated gradient of low to moderate POC flux, we find lowest taxon richness at the lowest POC flux, as well as an effect of nodule size on community composition. Our results suggest that while abyssal seamounts are important reservoirs of metazoan diversity in the CCZ, given limited taxonomic overlap between seamount and plains fauna, conservation of seamount assemblages will be insufficient to protect biodiversity and ecosystem function in regions targeted for mining.

Assessing biodiversity shortfalls of freshwater meiofauna from the Atlantic Forest: New species, distribution patterns and the first total-evidence phylogeny of semiplanktonic Gastrotricha.

The Brazilian Atlantic forest is a tropical rainforest recognized as a hotspot of biodiversity, with high species richness and endemicity. This forest extends over a wide latitudinal range, bordering the entire Brazilian coastline, from sea level to high mountains over 2000 m.a.s.L., and presents a variety of environmental conditions and forest physiognomy. Despite many years of intense studies on animal biodiversity in the biome, there is a lack of information on meiofauna taxa causing several shortfalls in biodiversity knowledge of these tiny organisms. In this study, we address some of these shortfalls by describing a new species of Neogossea (Gastrotricha: Chaetonotida) from a lentic ecosystem in southeastern Brazil, surrounded by fragments of Atlantic Forest by using an integrative approach combining different morphological techniques and molecular data. We also point out new hypotheses of homologous structures due to scanning electron microscope observations of the new species. Additionally, we used two numerical methods to assess distribution patterns and historical regionalization of four freshwater meiofaunal taxa (Gastrotricha, Rotifera, Copepoda and Cladocera). For the first time, we accessed the areas of endemism in this biological hotspot based on aquatic fauna with a very peculiar life history. Due to sampling issues and meiofauna species being widespread, our results raise incongruences with previous endemism analyses on vertebrates and arthropods. Finally, we performed the first total-evidence phylogenetic analyses of benthic and semiplanktonic gastrotrichs based on 59 morphological characters and three molecular markers, employing a parsimony approach. The phylogenetic reconstruction supports the hypothesis of a single origin of semiplanktonic gastrotrichs, and both Dasydytidae and Neogosseidae families are monophyletic taxa as well as four non-monotypic genera.

A New Species of Ostracod (Crustacea) Associated with a Feather Star: First Report of Ostracoda from Crinoidea.

We describe Obesostoma crinophilum sp. nov. (Ostracoda: Podocopida: Paradoxostomatidae) obtained from the body surface of the feather star Antedon serrata A. H. Clark, 1908 (Crinoidea: Comatulida: Antedonidae). This is the first report of Ostracoda associated with Crinoidea. None of the highly specialized appendages and/or carapace that are related to a commensal lifestyle were observed in O. crinophilum sp. nov. Therefore, the relationship between O. crinophilum sp. nov. and A. serrata must be transient rather than obligatory. However, O. crinophilum sp. nov. has a more developed hook-like distal claw on the antenna in comparison with four previously known Obesostoma species. The relatively well-developed distal claw of the antenna in O. crinophilum sp. nov. should indicate its intimate association with feather stars, though the feeding habit is still unknown.

Quantitative food web modeling unravels the importance of the microphytobenthos-meiofauna pathway for a high trophic transfer by meiofauna in soft-bottom intertidal food webs.

Meiofauna are known to have an important role on many ecological processes, although, their role in food web dynamics is often poorly understood, partially as they have been an overlooked and under sampled organism group. Here, we used quantitative food web modeling to evaluate the trophic relationship between meiofauna and their food sources and how meiofauna can mediate the carbon flow to higher trophic levels in five contrasting soft-bottom intertidal habitats (including seagrass beds, mudflats and sandflats). Carbon flow networks were constructed using the linear inverse model-Markov chain Monte Carlo technique, with increased resolution of the meiofauna compartments (i.e. biomass and feeding ecology of the different trophic groups of meiofauna) compared to most previous modeling studies. These models highlighted that the flows between the highly productive microphytobenthos and the meiofauna compartments play an important role in transferring carbon to the higher trophic levels, typically more efficiently so than macrofauna. The pathway from microphytobenthos to meiofauna represented the largest flow in all habitats and resulted in high production of meiofauna independent of habitat. All trophic groups of meiofauna, except for selective deposit feeders, had a very high dependency on microphytobenthos. Selective deposit feeders relied instead on a wider range of food sources, with varying contributions of bacteria, microphytobenthos and sediment organic matter. Ecological network analyses (e.g. cycling, throughput and ascendency) of the modeled systems highlighted the close positive relationship between the food web efficiency and the assimilation of high-quality food sources by primary consumers, e.g. meiofauna and macrofauna. Large proportions of these flows can be attributed to trophic groups of meiofauna. The sensitivity of the network properties to the representation of meiofauna in the models leads to recommending a greater attention in ecological data monitoring and integrating meiofauna into food web models.

Future ocean climate homogenizes communities across habitats through diversity loss and rise of generalist species.

Predictions of the effects of global change on ecological communities are largely based on single habitats. Yet in nature, habitats are interconnected through the exchange of energy and organisms, and the responses of local communities may not extend to emerging community networks (i.e., metacommunities). Using large mesocosms and meiofauna communities as a model system, we investigated the interactive effects of ocean warming and acidification on the structure of marine metacommunities from three shallow-water habitats: sandy soft-bottoms, marine vegetation, and rocky reef substrates. Primary producers and detritus-key food sources for meiofauna-increased in biomass under the combined effect of temperature and acidification. The enhanced bottom-up forcing boosted nematode densities but impoverished the functional and trophic diversity of nematode metacommunities. The combined climate stressors further homogenized meiofauna communities across habitats. Under present-day conditions metacommunities were structured by habitat type, but under future conditions they showed an unstructured random pattern with fast-growing generalist species dominating the communities of all habitats. Homogenization was likely driven by local species extinctions, reducing interspecific competition that otherwise could have prevented single species from dominating multiple niches. Our findings reveal that climate change may simplify metacommunity structure and prompt biodiversity loss, which may affect the biological organization and resilience of marine communities.

Morphology of the nervous system of monogonont rotifer Epiphanes senta with a focus on sexual dimorphism between feeding females and dwarf males.

BACKGROUND: Monogononta is a large clade of rotifers comprised of diverse morphological forms found in a wide range of ecological habitats. Most monogonont species display cyclical parthenogenesis, where generations of asexually reproducing females are interspaced by mixis events when sexual reproduction occurs between mictic females and dwarf, haploid males. The morphology of monogonont feeding females is relatively well described, however data on male anatomy are very limited. Thus far, male musculature of only two species has been described with confocal laser scanning microscopy (CLSM) and it remains unknown how dwarfism influences the neuroanatomy of males on detailed level. RESULTS: Here, we provide a CLSM-based description of the nervous system of both sexes of Epiphanes senta, a freshwater monogonont rotifer. The general nervous system architecture is similar between males and females and shows a similar level of complexity. However, the nervous system in males is more compact and lacks a stomatogastric part. CONCLUSION: Comparison of the neuroanatomy between male and normal-sized feeding females provides a better understanding of the nature of male dwarfism in Monogononta. We propose that dwarfism of monogonont non-feeding males is the result of a specific case of heterochrony, called "proportional dwarfism" as they, due to their inability to feed, retain a juvenile body size, but still develop a complex neural architecture comparable to adult females. Reduction of the stomatogastric nervous system in the males correlates with the loss of the entire digestive tract and associated morphological structures.

Diurodrilus kunii sp. nov. (Annelida: Diurodrilidae) and a Molecular Phylogeny of the Genus.

A new species of stygobiontic interstitial annelid, Diurodrilus kunii sp. nov., is described based on material collected from medium sand sediment (ϕ = 1.2-1.7) at groundwater level (40-100 cm in depth; 5-15 m inland from splash zone) in the intertidal beach slope on Ishikari Beach, facing the Sea of Japan, Hokkaido, Japan. The new species differs from six known congeners in the arrangement of the anterior-head ventral ciliophores, the degree of development in the primary and secondary toes, and the shape of the spermatozoa. We inferred the phylogenetic position of the new species among other congeners for which 18S rRNA, 28S rRNA, and COI gene sequences were available in public databases. This is the first representative of the genus from the Northwest Pacific.

Is the spatial distribution of lentic water mite assemblages (Acari: Hydrachnidia) governed by prey availability?

Predation is a biotic interaction that links water mites to different taxonomic groups of benthic invertebrates. Diptera larvae (Chironomidae) and microcrustaceans (Cladocera, Copepoda, Ostracoda) are known to be the most commonly preferred prey by water mites. Although these predatory interactions are known from observations and ex situ investigations, the distribution patterns and co-occurrence of water mites and their prey in littoral lentic habitats have been poorly studied. Our goal was to determine whether predation might serve as a significant factor in water mite assemblage composition and distribution. Samples were taken from littoral zones of 21 artificial lakes (reservoirs) in the Dinaric Western Balkan ecoregion of Croatia. At every site, 10 samples were collected with regard to shore slope in depth zones of up to 1 m. In total 490 samples were collected from April 2016 to July 2017. Data analysis showed that the spatial distribution of water mites was partially correlated with the environmental parameters we measured (explaining 45.1% of total water mite variation). A positive correlation between water mite abundance and diversity and depth gradient (favouring more stable conditions in deeper parts of the littoral zone) was also observed. Finally, water mite abundance and species richness variation were found to be best predicted by the abundance of potential prey groups. Predation was statistically determined to be a strong dispersion variable, most probably influencing both spatial distribution and composition of water mite assemblages.

Species diversity in the marine microturbellarian Astrotorhynchus bifidus sensu lato (Platyhelminthes: Rhabdocoela) from the Northeast Pacific Ocean.

Increasing evidence suggests that many widespread species of meiofauna are in fact regional complexes of (pseudo-)cryptic species. This knowledge has challenged the 'Everything is Everywhere' hypothesis and also partly explains the meiofauna paradox of widespread nominal species with limited dispersal abilities. Here, we investigated species diversity within the marine microturbellarian Astrotorhynchus bifidus sensu lato in the Northeast Pacific Ocean. We used a multiple-evidence approach combining multi-gene (18S, 28S, COI) phylogenetic analyses, several single-gene and multi-gene species delimitation methods, haplotype networks and conventional taxonomy to designate Primary Species Hypotheses (PSHs). This included the development of rhabdocoel-specific COI barcode primers, which also have the potential to aid in species identification and delimitation in other rhabdocoels. Secondary Species Hypotheses (SSHs) corresponding to morphospecies and pseudo-cryptic species were then proposed based on the minimum consensus of different PSHs. Our results showed that (a) there are at least five species in the A. bifidus complex in the Northeast Pacific Ocean, four of which can be diagnosed based on stylet morphology, (b) the A. bifidus complex is a mixture of sympatric and allopatric species with regional and/or subglobal distributions, (c) sympatry occurs on local (sample sites), regional (Northeastern Pacific) and subglobal (Northern Atlantic, Arctic, Northeastern Pacific) scales. Mechanisms for this co-occurrence are still poorly understood, but we hypothesize they could include habitat differentiation (spatial and/or seasonal) and life history characteristics such as sexual selection and dispersal abilities. Our results also suggest the need for improved sampling and exploration of molecular markers to accurately map gene flow and broaden our understanding of species diversity and distribution of microturbellarians in particular and meiofauna in general.

Will the Antarctic tardigrade Acutuncus antarcticus be able to withstand environmental stresses related to global climate change?

Because conditions in continental Antarctica are highly selective and extremely hostile to life, its biota is depauperate, but well adapted to live in this region. Global climate change has the potential to impact continental Antarctic organisms because of increasing temperatures and ultraviolet radiation. This research evaluates how ongoing climate changes will affect Antarctic species, and whether Antarctic organisms will be able to adapt to the new environmental conditions. Tardigrades represent one of the main terrestrial components of Antarctic meiofauna; therefore, the pan-Antarctic tardigrade Acutuncus antarcticus was used as model to predict the fate of Antarctic meiofauna threatened by climate change. Acutuncus antarcticus individuals tolerate events of desiccation, increased temperature and UV radiation. Both hydrated and desiccated animals tolerate increases in UV radiation, even though the desiccated animals are more resistant. Nevertheless, the survivorship of hydrated and desiccated animals is negatively affected by the combination of temperature and UV radiation, with the hydrated animals being more tolerant than desiccated animals. Finally, UV radiation has a negative impact on the life history traits of successive generations of A. antarcticus, causing an increase in egg reabsorption and teratological events. In the long run, A. antarcticus could be at risk of population reductions or even extinction. Nevertheless, because the changes in global climate will proceed gradually and an overlapping of temperature and UV increase could be limited in time, A. antarcticus, as well as many other Antarctic organisms, could have the potential to overcome global warming stresses, and/or the time and capability to adapt to the new environmental conditions.

DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity.

Documenting the diversity of marine life is challenging because many species are cryptic, small, and rare, and belong to poorly known groups. New sequencing technologies, especially when combined with standardized sampling, promise to make comprehensive biodiversity assessments and monitoring feasible on a large scale. We used this approach to characterize patterns of diversity on oyster reefs across a range of geographic scales comprising a temperate location [Virginia (VA)] and a subtropical location [Florida (FL)]. Eukaryotic organisms that colonized multilayered settlement surfaces (autonomous reef monitoring structures) over a 6-mo period were identified by cytochrome c oxidase subunit I barcoding (>2-mm mobile organisms) and metabarcoding (sessile and smaller mobile organisms). In a total area of ∼ 15.64 m(2) and volume of ∼ 0.09 m(3), 2,179 operational taxonomic units (OTUs) were recorded from 983,056 sequences. However, only 10.9% could be matched to reference barcodes in public databases, with only 8.2% matching barcodes with both genus and species names. Taxonomic coverage was broad, particularly for animals (22 phyla recorded), but 35.6% of OTUs detected via metabarcoding could not be confidently assigned to a taxonomic group. The smallest size fraction (500 to 106 µm) was the most diverse (more than two-thirds of OTUs). There was little taxonomic overlap between VA and FL, and samples separated by ∼ 2 m were significantly more similar than samples separated by ∼ 100 m. Ground-truthing with independent assessments of taxonomic composition indicated that both presence-absence information and relative abundance information are captured by metabarcoding data, suggesting considerable potential for ecological studies and environmental monitoring.