Prevalence of Escherichia coli in electrogenic biofilm on activated carbon in microbial fuel cell.

For a better understanding of the distribution of depth-dependent electrochemically active bacteria at in the anode zone, a customized system in a microbial fuel cell (MFC) packed with granular activated carbon (GAC) was developed and subsequently optimized via electrochemical tests. The constructed MFC system was sequentially operated using two types of matrice solutions: artificially controlled compositions (i.e., artificial wastewater, AW) and solutions obtained directly from actual sewage-treating municipal plants (i.e., municipal wastewater, MW). Notably, significant difference(s) of system efficiencies between AW or MW matrices were observed via performance tests, in that the electricity production capacity under MW matrices is < 25% that of the AW matrices. Interestingly, species of Escherichia coli (E. coli) sampled from the GAC bed (P1: deeper region in GAC bed, P2: shallow region of GAC near electrolytes) exhibited an average relative abundance of 75 to 90% in AW and a relative abundance of approximately 10% in MW, while a lower relative abundance of E. coli was found in both the AW and MW anolyte samples (L). Moreover, similar bacterial communities were identified in samples P1 and P2 for both the AW and MW solutions, indicating a comparable distribution of bacterial communities over the anode area. These results provide new insights into E. coli contribution in power production for the GAC-packed MFC systems (i.e., despite the low contents of Geobacter (> 8%) and Shewanella (> 1%)) for future applications in sustainable energy research. KEY POINTS: • A microbial community analysis for depth-dependence in biofilm was developed. • The system was operated with two matrices; electrochemical performance was assessed. • E. coli spp. was distinctly found in anode zone layers composed of activated carbon.

Impacts of the Ban on the Soil-Applied Fumigant Methyl Bromide.

The loss of the soil fumigant methyl bromide (MeBr) and adoption of soil fumigant alternatives has been challenging for farmers, particularly for those crops in which pathogens previously controlled by MeBr have emerged as significant problems, but it has resulted in some unanticipated benefits for the scientific community and the environment. Applauded as one of the most effective environmental agreements to date, the universally accepted Montreal Protocol on Ozone Depleting Substances has had a significant impact on the environment, reducing the release of halogenated compounds from anthropogenic sources enough to mitigate global warming by an estimated 1.1°C by 2021. The funding associated with various MeBr transition programs has increased collaboration across scientific disciplines, commodity groups, industry, and regulatory agencies. Chemical alternatives and improved application strategies, including the development of gas-retentive agricultural films, coupled with sound efficacy data and grower ingenuity have resulted in the sustained production of many of the impacted crops; although there has been some loss of acreage and value, particularly for Florida fumigated crops, for some, value has continued to increase, allowing production to continue. The loss of a single, broad-spectrum tool for pest control has led to a deeper understanding of the specific pest complexes impacting these at-risk crops, as well as the development of new, biologically based management tools for their control while increasing our understanding of the role of the soil microbiome in pest control and crop production.

Timeframe of aerobic biodegradation of bioplastics differs under standard conditions and conditions simulating technological composting with biowaste.

To determine the actual timeframe of biodegradation, bioplastics (BPs) (based on polylactic acid (PLA), starch (FS), polybutylene succinate (PBS), cellulose (Cel)) were degraded with biowaste (B), which simulates real substrate technological conditions during composting. For comparison, standard conditions (with mature compost (C)) were also applied. The 90-day aerobic tests, both with C or B, were carried out at 58 ± 2 °C. This comparison enables understanding of how BPs behave in real substrate conditions and how C and B affect the time or completeness of degradation based on oxygen consumption (OC) for BPs, the ratio of OC to theoretical oxygen consumption (OC/Th-O2), and the decrease in volatile solids (VS). Additionally, for deeper insight into the biodegradation process, microscopic, microbial (based on 16S rDNA), FTIR, and mechanical (tensile strength, elongation at break) analyses were performed. There was no association between the initial mechanical properties of BPs and the time necessary for their biodegradation. BPs lost their mechanical properties and remained visible for a shorter time when degraded with C than with B. OC for Cel, FS, PLA, and PBS biodegradation was 1143, 1654, 1748, and 1211g O2/kg, respectively, which amounted to 83, 70, 69, and 60% of the theoretical OC (Th-O2), respectively. Intensive OC took place at the same time as an intensive decrease in VS content. With C, Cel was most susceptible to biodegradation (completely biodegrading within 11 days), and PLA was least susceptible (requiring 70 days for complete biodegradation). With B, however, the time required for biodegradation was generally longer, and the differences in the time needed for complete biodegradation were smaller, ranging from 45 d (FS) to 75 d (PLA). The use of C or B had the greatest effect on Cel biodegradation (10 d vs 62 d, respectively), and the least effect on PLA (70 d vs 75 d). Specific bacterial and fungal community structures were identified as potential BP biodegraders; the communities depended on the type of BPs and the substrate conditions. In conclusion, the time needed for biodegradation of these BPs varied widely depending on the specific bioplastic and the substrate conditions; the biodegradability decreased in the following order: Cel â‰« FS â‰« PBS â‰« PLA with C and FS â‰« Cel = PBS â‰« PLA with B. The biodegradability ranking of BPs with B was assumed to be ultimate as it simulates the real substrate conditions during composting. However, all of the BPs completely biodegraded in less than 90 days.

Performance of a novel Built-in Static Magnetic Field - Biological Aerated Filter (BSMF-BAF) for treating high-salt textile dyeing wastewater.

High-salt textile dyeing wastewater is difficult to treat. Magnetic fields can enhance the biodegradation capacity and extreme environmental adaptabilities of microorganisms. Thus, magnetically enhanced bioreactors are expected to improve the treatment efficiency and stability of high-salt textile dyeing wastewater. Accordingly, a novel Built-in Static Magnetic Field - Biological Aerated Filter (BSMF-BAF) was constructed and investigated for treating actual high-salt textile dyeing wastewater in this study. Two other BAFs packed with traditional and magnetic ceramsite carriers, respectively, were simultaneously operated for comparison. The removal of color, chemical oxygen demand (COD), suspended solid (SS) and acute toxicity were monitored. The activities of key enzymes and microbial community structure were analyzed to reveal possible mechanisms for improving the treatment efficiency of traditional BAF using the BSMF. The results showed that the BSMF-BAF possessed the highest removal efficiencies of color, COD, SS and acute toxicity among the three BAFs. The BSMF induced significant increases in the activities of azoreductase and lignin peroxidase, which were responsible for the degradation of azo compounds in the wastewater and the detoxification of toxic intermediates, respectively. Additionally, the BSMF induced the relative enrichment of potentially effective bacteria and fungi, and it maintained a relatively high abundance of fungi in the microbial community, resulting in a high treatment efficiency.

Effects of Graphene Oxide on Endophytic Bacteria Population Characteristics in Plants from Soils Contaminated by Polycyclic Aromatic Hydrocarbons.

Environmental pollution stands as one of the significant global challenges we face today. Polycyclic aromatic hydrocarbons (PAHs), a class of stubborn organic pollutants, have long been a focal point of bioremediation research. This study aims to explore the impact and mechanisms of graphene oxide (GO) on the phytoremediation effectiveness of PAHs. The results underscore the significant efficacy of GO in accelerating the degradation of PAHs. Additionally, the introduction of GO altered the diversity and community structure of endophytic bacteria within the roots, particularly those genera with potential for PAH degradation. Through LEfSe analysis and correlation studies, we identified specific symbiotic bacteria, such as Mycobacterium, Microbacterium, Flavobacterium, Sphingomonas, Devosia, Bacillus, and Streptomyces, which coexist and interact under the influence of GO, synergistically degrading PAHs. These bacteria may serve as key biological markers in the PAH degradation process. These findings provide new theoretical and practical foundations for the application of nanomaterials in plant-based remediation of polluted soils and showcase the immense potential of plant-microbe interactions in environmental restoration.

Temporal dynamic of antibiotic resistance genes in the Zaohe-Weihe hyporheic zone: driven by oxygen and bacterial community.

The widespread spread of antibiotic resistance genes (ARGs) in hyporheic zone (HZ) has become an emerging environmental problem due to their potentially harmful nature. In this research, three different oxygen treatment systems were set up to study the effects of oxygen changes on the abundance of ARGs in the HZ. In addition, the effects of temperature and salinity on ARGs were investigated under aerobic and anaerobic systems, respectively. The bacterial community composition of sediment samples and the relationship with ARGs were analyzed. The explanation ratio and causality of the driving factors affecting ARGs were analyzed using variation partitioning analysis (VPA) and structural equation model (SEM). The relative abundance of ARGs and mobile genetic elements (MGEs) in the anaerobic system increased significantly, which was higher than that in the aerobic system and the aerobic-anaerobic interaction system. The experiment of salinity and temperature also further proved this result. There were many bacterial communities that affected tetracycline and sulfonamide ARGs in sediments, and these host bacteria are mainly concentrated in Proteobacteria, Firmicutes and Bacteroidetes. VPA and SEM further revealed that the abundance of ARGs was mainly influenced by changes in bacterial communities and oxygen conditions, and horizontal gene transfer (HGT) of MGEs also had a positive effect on the spread of ARGs. Those findings suggest that complex oxygen conditions in the HZ alter bacterial communities and promote MGEs-mediated horizontal transfer, which together lead to the spread of ARGs. This study has value as a reference for formulating effective strategies to minimize the propagation of ARGs in underground environment.

Treatment of phenolic wastewater by anaerobic fluidized bed microbial fuel cell using carbon brush as anode: microbial community analysis and m-cresol degradation mechanism.

Anaerobic fluidized bed microbial fuel cell (AFB-MFC) is a technology that combines fluidized bed reactor and microbial fuel cell to treat organic wastewater and generate electricity. The performance and the mechanism of treating m-cresol wastewater in AFB-MFC using carbon brush as biofilm anode were studied. After 48 h of operation, the m-cresol removal efficiency of AFB-MFC, MAR-AFB (fluidized bed bioreactor with acclimated anaerobic sludge), MAR-FB (ordinary fluidized bed reactor with only macroporous adsorptive resin) and AST (traditional anaerobic sludge treatment) were 95.29 ± 0.67%, 85.78 ± 1.81%, 71.24 ± 1.86% and 70.41 ± 0.32% respectively. The maximum output voltage and the maximum power density of AFB-MFC using carbon brush as biofilm anode were 679.7 mV and 166.6 mW/m2 respectively. The results of high-throughput sequencing analysis indicated the relative abundance of dominant electroactive bacteria, such as Trichococcus, Geobacter, and Pseudomonas, on the anode carbon brushes was higher than that of AST, and also identified such superior m-cresol-degrading bacteria as Bdellovibrio, Thermomonas, Hydrogenophaga, etc. Based on the determination of m-cresol metabolites detected by Gas Chromatography-Mass Spectrometry (GC-MS), the possible biodegradation pathway of m-cresol under anaerobic and aerobic conditions in AFB-MFC was speculated. The results showed that m-cresol was decomposed into formic acid-acetic anhydride and 3-methylpropionic acid under the action of electrochemistry, which is a simple degradation pathway without peripheral metabolism in AFB-MFC.

Response of methanogenic system to long-term polycyclic aromatic hydrocarbon exposure: Adsorption and biodegradation, performance variation, and microbial function assessment.

Phenanthrene (PHE) as a typical polycyclic aromatic hydrocarbon (PAH) is prevalent and harmful to organisms in petroleum-polluted sites. The effects of PHE concentration levels on performance, microbial community and functions in methanogenic system were comprehensively investigated by an operation of UASB reactor (198 days) and a series of batch tests. The results found that PHE was prone to accumulate in reactor by sludge adsorption (Final concentration = 12.53 mg/g TS Sludge), which posed significant influences on methanogenic system. The removal of chemical oxygen demand (COD), NH4+-N and volatile fatty acids (VFAs) in reactor were reduced with PHE accumulation. Meanwhile, microbes with higher ATPase secrete more EPS activity to self-protect against PHE toxicity. Sequencing analysis showed that PHE interfered significantly diversity and structure of microbial community. For bacteria, PHE was toxic to Bacteroidetes and Latescibacteria, while syntrophs (f_Syntrophaceae, Syntrophorhabdus, etc.) involved in VFAs oxidation and aromatic organics degradation were tolerant of PHE stress. For archaea, acetoclastic methanogens (Methanosaeta) abundance was continuously diminished by 45.1% under long-term PHE exposure. Further functions analysis suggested that microbial community accelerated amino acid metabolism, energy metabolism and xenobiotics biodegradation & metabolism to satisfy physiological demanding under PHE stress. Combining batch tests of methanogenic metabolism proved that acetoclastic methanogenesis was negatively affected by PHE due to inhibition of functional enzymes (acetate kinase, phosphate acetyltransferase, etc.) expression. These findings may provide the basis for enhancing bioremediation of PAH pollution in anaerobic environment.

Effect of polystyrene nanoplastics on the activated sludge process performance and biomass characteristics. A laboratory study with a sequencing batch reactor.

The fate and presence of nanoplastics in wastewater treatment systems is a topic of increasing interest. Furthermore, challenges related to their quantification and identification have made it difficult to set up experimental conditions and compare results between studies. In this study, the effect of 100 nm polystyrene nanoplastics on activated sludge was evaluated. A concentration of 2 µg/L was used to continuously feed a sequencing batch reactor (SBR-NPs). Under the experimental conditions used in this study, no changes were observed in the process performance of the SBR-NPs compared to the reactor used as a control. Neither nitrification nor organic matter removal efficiency, which was 96% for both SBRs, were affected by the presence of 100 nm polystyrene nanoplastics, which suggests that the tested nanoplastics were not sufficiently toxic to the biomass. Although no significant differences in the relative abundances of predominant phyla between SBR-Control and SBR-NPs were observed, a slight shift in the relative abundance of Patescibacteria (1.5 ± 0.6% and 3.7 ± 0.8% in SBR-Control and SBR-NPs, respectively, at the end of the test) occurred. The higher abundance of this phylum in SBR-NPs compared to SBR-Control may suggest that these bacteria have some sensitivity to the presence of 100 nm polystyrene nanoplastics. Furthermore, even with the absence of nitrification inhibition, it was observed stagnation of the growth of Nitrotoga bacteria in SBR-NPs, which also suggests that the polystyrene nanoplastics could have an inhibitory effect on these cells and an impact on nitrification in the long term.

Continuous operation of fungal wheel reactor based on solid-state fermentation for the removal of pharmaceutical and personal care products.

Wood-rotting fungi and their enzymatic systems represent promising biocatalysts for the removal of pharmaceuticals and personal care products (PPCPs) from wastewater. We designed a fungal wheel reactor (FWR) based on solid-state fermentation (SSF) of Trametes versicolor and a lignocellulosic substrate, which was used as an immobilization carrier for fungal biomass and the sole initial nutrient source for producing fungal oxidative enzymes. Three pharmaceutical and personal care products, acetaminophen, bisphenol A and carbamazepine, were spiked into the synthetic wastewater and the treatment was carried out under non-sterile conditions. Acetaminophen was completely removed from the FWR until laccase was observed. The acetaminophen removal efficiency was retrieved by replacing the fungal wheel with fresh SSF products. Bisphenol A and carbamazepine were removed via enzymatic activity and adsorption. When the fungal wheel was replaced, acetaminophen began to be completely removed, even after laccase depletion. The microbial community analysis indicated that the continuous removal of acetaminophen was mainly due to the high proportion of T. versicolor. The relative abundance of the co-occurring microbial community might be responsible for the divergence in acetaminophen removal between two of fungal wheel-replaced reactors. Overall, FWRs are promising tools for the removal of PPCPs by highly reactive enzymatic mechanisms as well as adsorption on the carrier surface. By replacing SSF and settled microbial communities, FWRs may continuously contribute to bioremediation over a long-term period.

Microbial community profiling by next-generation DNA sequencing of adenocarcinoma of the prostate with evidence of ochratoxin A producing fungi.

BACKGROUND: Prostatic carcinomas are a leading cancer and leading cause of mortality in the developed world. The etiology is diverse with underlying patient genetics, environmental factors, and microbial associations. Sequencing DNA for microbes allows the detection of potential disease relationships. OBJECTIVE: Targeted 16S (prokaryotic) and 18S (eukaryotic) rDNA sequencing was performed to map the tumor microbial flora. DESIGN: Twelve patients undergoing elective laparoscopic prostatectomy for biopsy proven adenocarcinoma of the prostate were enrolled. PCR and amplicon based sequencing was conducted; a portion of the sequencing results were confirmed by special stains. SETTING: Patients were recruited by the urologist were prospectively scheduled for radical prostatectomy by 'Da Vinci' robotically assisted procedure in an outpatient setting. Samples were portioned in the hospital surgical suite at the time of prostatectomy. PARTICIPANTS: Male patients were requested to enter the study on a first come basis. OUTCOME MEASUREMENT AND STATISTICAL ANALYSIS: Average age of the 12 participants was 64.3 years. RESULTS AND LIMITATIONS: DNA reads were detected and by 'best match' were identified belonging to Perkinsus, Hydrurus, Diversispora and Funneliformis genera, few samples displayed bacteria. Out of the 12 total patients, 11 patients had detectable DNA sequences matching arbuscular mycorrhizal fungi in the Glomeromycetes Class; Funneliformis mosseae and Diversasporum versiformis. Specific PCR for arbuscular mycorrhizal fungi failed to confirm Glomeromycetes Class; in-depth taxonomic analysis suggests a newer fungal grouping, not falling within an accepted Phylum of fungi. Calcoflour white staining of histological sections confirmed potential fungal markers in all 12 cases. Ochratoxin A antigen was identified by immunofluorescence in all 12 patient samples. The study was limited by the low sample volume and disease free normal controls. CONCLUSIONS: Fungi may play a significant role in adenocarcinoma of the prostate.

Biocathode regulates enrofloxacin degradation by coupling with different co-metabolism conditions.

In this study, biocathode system coupled with different co-metabolism conditions (NaAc, glucose and NaHCO3) were developed to degrade quinolones enrofloxacin (ENR) due to its poorly metabolization, easily accumulation and potential toxicity. Simultaneously, ENR reduction kinetic rate constant in NaAc-fed, glucose-fed and NaHCO3-fed biocathodes, and sole biocathode were increased by 343.62%, 320.46%, 189.19% and 130.88% when compared with that of abiotic cathode when the operational time and ENR concentration were set to 48 h and 25 mg/L. In addition, transformation pathways of ENR revealed pathway II were dominantly occurred in NaAc- and glucose-fed biocathode while pathway IV acting as key metabolic process were shown in NaHCO3-fed biocathode. Moreover, 16S rRNA high-throughput sequencing analysis indicated that biocathodic communities were sensitive to switch-over of carbon source, namely Delftia and Bosea as organohalide-respiring bacteria (OHRB) were abundant in NaAc- and glucose-fed biocathodes while Mesotoga and Syntrophorhabdus that responsible for benzoyl-CoA metabolic process were enriched in NaHCO3-fed biocathode. Overall, this study could unravel the underlying relationship between biocathode degradation pattern of ENR and different co-metabolism conditions, and further offer valuable scientific information on treating refractory quinolones antibiotics via green bioelectrochemical method.

Performance optimization and microbial community evaluation for domestic wastewater treatment in a constructed wetland-microbial fuel cell.

Constructed wetland-microbial fuel cell system (CW-MFC), an attractive technology still under study, has shown to improve domestic wastewater treatment efficiency and generate bioelectricity. This work investigated the effect of multiple factors on the performance optimization for the pollutants removal and bioelectricity production compared to a traditional CW, including influent chemical oxygen demand (COD) concentration, hydraulic retention time (HRT) and external resistance. The results showed that the optimal operating conditions of COD concentration, HRT and external resistance for CW-MFC were 200 mg/L, 24 h and 1000 Ω, respectively. The average COD, NH4+-N, NO3--N and TP removal efficiencies were 6.06%, 3.85%, 3.68% and 3.68% higher than these in CW system, respectively. Meanwhile, the maximum output voltage and power density of CW-MFC were 388 ± 12 mV and 107.54 mW/m3. In addition, the microbial community analysis indicated that the pollution removal and bioelectricity generation might benefit from the gradual enrichment of electroactive bacteria (Tolumonas) and denitrifying bacteria (Denitratisoma, Methylotenera and Sulfuritales). The findings can provide the optimum operation parameters and mechanism insight for the performance of CW-MFC systems.

Strategy of optimizing anaerobic digestion of cassava distiller wastewater using a novel automatic biological incubation system.

Due to the drawbacks of using fossil fuels and the need to mitigate global warming caused by increasing greenhouse gas emissions, agricultural biomass for bioenergy production is gaining great interest around the world. This work presented a study at a biochemical plant in Lianyungang, Jiangsu Province, China to maximize methane production from cassava distiller wastewater. The plant's annual production of cassava distiller wastewater is more than 3 million tons and currently was treated using a series of 5000 m3 Internal Circulation (IC) reactors. Modification was applied at No.19 IC reactor by connecting it to two 1 m3 automatic biological incubators called Information Bio-Booster (IBB). The effluent of the IC reactor was fed into the IBBs and iron, cobalt and nickel were added directly in the IBBs. The function of the IBBs was to regulate the microbial community. Afterwards, the microorganisms in the IBBs were pumped back into the IC reactor to participate in the methane production reaction. Daily net increase of methane content and COD removal reached 8.02% and 33% respectively in No.19 IC reactor comparing to the unadjusted reactors. Preliminary lab experiments found that improvements of biogas production, enhanced COD removal and VS removal was closely related to the enhancement of anaerobic microbial communities' diversity and the promotion of enzyme activity through the addition of the metal salts. Daily economic value could be estimated to be $218 which indicated the application potential of using the proposed system to enhance anaerobic digestion at industrial plants for bioenergy production.

Accelerated stabilization of high solid sludge by thermal hydrolysis pretreatment in autothermal thermophilic aerobic digestion (ATAD) process.

Autothermal thermophilic aerobic digestion (ATAD) is a rapid biological treatment technology for sludge stabilization. To improve digestion efficiency and shorten stabilization time, thermal hydrolysis pretreatment was employed before ATAD of high solid sludge. The results showed that accelerated stabilization of high solid sludge (total solid = 10.1%) was achieved by thermal hydrolysis pretreatment with volatile solid removal efficiency of 40.3% after 8 days of ATAD, 11 days earlier than unpretreated sludge. The enhanced release and hydrolysis of intracellular organics resulted in a solubilization degree of 45.3%. The reduced sludge viscosity and improved fluidity after thermal hydrolysis facilitated mixing, aeration and organics degradation during ATAD. Excitation emission matrix analysis indicated that the fluorescence intensity of soluble microbial byproduct and tyrosine-like protein increased markedly after thermal hydrolysis and decreased after ATAD. The proportion of high molecular weight (MW > 10 kDa) substances in the supernatant increased significantly after thermal hydrolysis, while the low MW (MW < 1 kDa) substances decreased after ATAD. The significant difference in microbial composition between the pretreatment and control groups elucidated the accelerated sludge stabilization under thermal hydrolysis. This work provides an efficient and practical strategy to achieve rapid stabilization of high solid sludge.

Biochar assisted cellulose anaerobic digestion under the inhibition of dodecyl dimethyl benzyl ammonium chloride: Dose-response kinetic assays, performance variation, potential promotion mechanisms.

This study evaluated the inhibitory effect and mitigation strategy of dodecyl dimethyl benzyl ammonium chloride (DDBAC) suppression on anaerobic digestion. With the 12 h-suppression, only 16.64% of anaerobes were alive, and acetotrophic methanogens were significantly inhibited. As for batch test, DDBAC suppression significantly prolonged the start-up of systems and decreased the biogas production. In cellulose semi-continuous digestion process, the DDBAC suppression induced volatile fatty acids accumulation and pH decrease. However, the biochar amended reactor effectively mitigated the DDBAC suppression and achieved 370.5 mL/d·g-chemical-oxygen-demand biogas production. Moreover, 17.8% more protein in extracellular polymeric substances was secreted as the bio-barrier to defense the DDBAC suppression. Furthermore, microbial analysis showed that biochar addition selectively enriched directed interspecies electron transfer (DIET) participant bacteria (Anaerolineaceae and Syntrophomonas) and methanogens (Methanosaeta and Methanobacterium). Meanwhile, the potential metabolic pathway analysis showed that the abundance of amino acids and energy metabolism were increased 28% and 8%, respectively. The abundance of encoding enzyme related to hydrogenotrophic and acetotrophic methanogenesis enriched 1.88 times and 1.48 times, respectively. These results showed the performance and mechanisms involved in DIET establishment with ethanol stimulation biochar addition.

Decentralized high-strength wastewater treatment using a compact aerobic baffled bioreactor.

Decentralized wastewater treatment is a potential solution to the economic and sustainability issues associated with current wastewater infrastructure requirements for rural and expanding urban settings. However, wastewater produced from small-scale operations (domestic, industrial, agricultural, defense, etc.) are often found to have higher pollution concentrations than municipal wastewater and is referred to as "blackwater" in the study. A baffled bioreactor (BBR) was employed to carry out blackwater treatment. The results of this study showed the removal of organic content and inorganic nitrogen was high in all of the feed amounts tested and increased corresponding to an increase in feed amount. Microbial diversity results supported that the feed amount was the most important factor in treatment performance. The microbial community was more diverse at higher feed amounts than lower feed amounts. A non-metric multidimensional scaling (NMDS) analysis revealed that higher feed amounts enriched Verrucomicrobiaceae, unclassified Sphingomonadales, unclassified Burkholderiales in the microbial community, and facilitated the removal of total solids, total nitrogen, and NO3-. The energy and exergy analyses showed the consumption of 5.02 Wh/L wastewater energy with a universal exergy efficiency of 61% at the feed amount of 3750 LPD. An economic analysis further delineated the treatment cost profiles of the decentralized unit under four different energy case scenarios of electricity from the grid, propane gas engine for remote rural communities, diesel engine (I) using standard U.S. market diesel fuel costs for remote rural communities and scientific research bases, and diesel engine (II) using the fully burdened cost of diesel fuel for military contingency bases and other extreme environmental scenarios.

Electrolysis-sulfate-reducing up-flow sludge bed-biological contact oxidation reactor for Norfloxacin removal from wastewater with high sulfate content.

This study investigated the treatment of 100-mg/L Norfloxacin (NOR) wastewater containing high concentrations of sulfate through a combination of electrolysis, sulfate-reducing up-flow sludge bed (SRUSB), and biological contact oxidation reactor (BCOR) treatments. Results revealed that after 62 h, the reaction system had processed over 97% of the NOR. Additionally, electrolysis with sodium sulfate as the electrolyte transformed 87.8% of the NOR but only 33.5% of the total organic carbon (TOC). In the SRUSB, the TOC and SO42- contents were simultaneously reduced by 87.4% and 95.6%, respectively, providing a stable environment to the BCOR. In the BCOR, 36.3% and 85.9% of the NOR and TOC were degraded. High-performance liquid chromatography-tandem mass spectrometry analysis identified three possible degradation pathways under the attack of -OH during electrolysis, including defluorination, piperazinyl ring transformation, and quinolone ring transformation. Furthermore, the Illumina HiSeq sequencing results demonstrated that the sulfate-reducing bacteria (represented by Desulfobacter and Desulfobulbus) in the SRUSB and the sulfate-oxidizing bacteria (mainly consisting of Gammaproteobacteria and Alphaproteobacteria) in the BCOR played important roles in carbon chain oxidation and benzene ring opening and thoroughly degraded the electrolysis products. Thus, this method effectively overcomes the incomplete degradation and low removal efficiency issues associated with single electrolysis or biological methods in traditional processes.

Identity and hydrocarbon degradation activity of enriched microorganisms from natural oil and asphalt seeps in the Kurdistan Region of Iraq (KRI).

A previous cultivation-independent investigation of the microbial community structure of natural oil and asphalt seeps in the Kurdistan Region of Iraq (KRI) revealed the dominance of uncultured bacterial taxa belonging to the phyla Deferribacterota and Coprothermobacterota and the orders Thermodesulfobacteriales, Thermales, and Burkholderiales. Here we report on a cultivation-dependent approach to identify members of these groups involved in hydrocarbon degradation in the KRI oil and asphalt seeps. For this purpose, we set up anoxic crude oil-degrading enrichment cultures based on cultivation media known to support the growth of members of the above-mentioned taxonomic groups. During 100-200 days incubation periods, nitrate-reducing and fermentative enrichments showed up to 90% degradation of C8-C17 alkanes and up to 28% degradation of C18-C33 alkanes along with aromatic hydrocarbons. Community profiling of the enrichment cultures showed that they were dominated by diverse bacterial taxa, which were rare in situ community members in the investigated seeps. Groups initially targeted by our approach were not enriched, possibly because their members are slow-growing and involved in the degradation of recalcitrant hydrocarbons. Nevertheless, the enriched taxa were taxonomically related to phylotypes recovered from hydrocarbon-impacted environments as well as to characterized bacterial isolates not previously known to be involved in hydrocarbon degradation. Marker genes (assA and bssA), diagnostic for fumarate addition-based anaerobic hydrocarbon degradation, were not detectable in the enrichment cultures by PCR. We conclude that hydrocarbon biodegradation in our enrichments occurred via unknown pathways and synergistic interactions among the enriched taxa. We suggest, that although not representing abundant populations in situ, studies of the cultured close relatives of these taxa will reveal an unrecognized potential for anaerobic hydrocarbon degradation, possibly involving poorly characterized mechanisms.

Design, application, and microbiome of sulfate-reducing bioreactors for treatment of mining-influenced water.

Sulfate-reducing bioreactors, also called biochemical reactors, represent a promising option for passive treatment of mining-influenced water (MIW) based on similar technology to aerobic/anaerobic-constructed wetlands and vertical-flow wetlands. MIW from each mine site has a variety of site-specific properties related to its treatment; therefore, design factors, including the organic substrates and inorganic materials packed into the bioreactor, must be tested and evaluated before installation of full-scale sulfate-reducing bioreactors. Several full-scale sulfate-reducing bioreactors operating at mine sites provide examples, but holistic understanding of the complex treatment processes occurring inside the bioreactors is lacking. With the recent introduction of high-throughput DNA sequencing technologies, microbial processes within bioreactors may be clarified based on the relationships between operational parameters and key microorganisms identified using high-resolution microbiome data. In this review, the test design procedures and precedents of full-scale bioreactor application for MIW treatment are briefly summarized, and recent knowledge on the sulfate-reducing microbial communities of field-based bioreactors from fine-scale monitoring is presented.Key points• Sulfate-reducing bioreactors are promising for treatment of mining-influenced water.• Various design factors should be tested for application of full-scale bioreactors.• Introduction of several full-scale passive bioreactor systems at mine sites.• Desulfosporosinus spp. can be one of the key bacteria within field-based bioreactors.