Long-term warming effects on the microbiome and nifH gene abundance of a common moss species in sub-Arctic tundra.

Bacterial communities form the basis of biogeochemical processes and determine plant growth and health. Mosses harbour diverse bacterial communities that are involved in nitrogen fixation and carbon cycling. Global climate change is causing changes in aboveground plant biomass and shifting species composition in the Arctic, but little is known about the response of moss microbiomes in these environments. Here, we studied the total and potentially active bacterial communities associated with Racomitrium lanuginosum in response to a 20-yr in situ warming in an Icelandic heathland. We evaluated the effect of warming and warming-induced shrub expansion on the moss bacterial community composition and diversity, and nifH gene abundance. Warming changed both the total and the potentially active bacterial community structure, while litter abundance only affected the total bacterial community structure. The abundance of nifH genes was negatively affected by litter abundance. We also found shifts in the potentially nitrogen-fixing community, with Nostoc decreasing and noncyanobacterial diazotrophs increasing in relative abundance. Our data suggest that the moss microbial community and potentially nitrogen fixing taxa will be sensitive to future warming, partly via changes in litter and shrub abundance.

Biomonitoring of transboundary pollutants using moss in Japan's mountains.

Long-range transported atmospheric pollutants (or transboundary pollutants) include trace metals with isotope ratios and compositions that vary from those of domestic pollutants, which threaten mountain ecosystems. These differences can be applied as indices to evaluate the influence of transboundary pollutants on mountain ecosystems. Mosses play important ecological functions in mountains and are sensitive to atmospheric deposition. Therefore, using these indices for moss biomonitoring can provide a more accurate indication of ecosystem health. However, studies on indices that are appropriate for moss biomonitoring are limited. Here, the effectiveness of moss biomonitoring using trace metal indices was examined to evaluate transboundary pollutants in mountainous areas in Japan. Transboundary pollutants in these areas originate from mainland Asia and are characterized by high lead isotope ratios, lead-to-zinc (Pb/Zn) ratios, and arsenic-to-vanadium (As/V) ratios. Given that the abundance of transboundary pollutants decreases with distance from mainland Asia, these three indices are also expected to vary with distance. The Pb isotope ratios were found to decrease with distance from mainland Asia; in contrast, the Pb/Zn and As/V ratios did not display any notable relationship with distance. These results are likely attributed to biological and environmental factors that affect trace metal content in moss. Thus, moss Pb isotope ratios are useful indicators of transboundary pollutants in Japan's mountains, offering an important tool for comparable moss biomonitoring studies in East Asia.

Physiological and growth responses of the montane bryophyte Racomitrium lanuginosum to atmospheric nitrogen deposition.

• The effects of nitrogen (N) deposition on the moss Racomitrium lanuginosum within montane heath in Scotland were investigated over 5 yr. • Permanent field plots were sprayed with KNO3 or NH4 Cl solutions, at doses equivalent to 10 and 40 kg N ha-1  yr-1 , in 3-6 applications each summer. • Racomitrium growth and cover were severely reduced by N addition, whilst the proportion of dead shoots greatly increased. N dose decreased inducibility of shoot nitrate reductase activity (NRA), suggesting that N saturation of Racomitrium occurred, and caused an increase in potassium leakage. At high dosage, effects of NH4 + were more detrimental than NO3 - . • Physiological responses to N indicate that the habitat's critical load (CL) is exceeded by addition of 10 kg N ha-1  yr-1 . The differential toxicity of the two forms of N suggests that predominant ion type in deposition should be taken into consideration when CLs are set. In contrast to tissue N, NRA correlated well with shoot growth, and may thus be a useful biological indicator of moss condition.