Cantharidin.

Cantharidin is the toxic component of blister beetles of the genus Epicauta. Cantharidin is a potent vesicant which causes blisters, erosions, and ulcerations in the gastrointestinal and urinary tracts, and can cause myocardial necrosis. Blister beetles are found over most of North America and specifically contaminate alfalfa at harvest. History of alfalfa feeding, with colic, dysuria, hypocalcemia, and hypomagnesemia are suggestive of blister beetle toxicosis. Myocardial damage causes increased serum cardiac troponin 1. Tentative diagnosis can be made by finding the beetles in feed or ingesta. Definitive diagnosis requires detection of cantharidin in urine or gastric contents. Treatment involves ending exposure, decreasing absorption, controlling pain, using gastroprotectants, and fluids and electrolyte replacement. Prognosis is guarded to poor.

Anticancer Attributes of Cantharidin: Involved Molecular Mechanisms and Pathways.

Cancer is a preeminent threat to the human race, causing millions of deaths each year on the Earth. Traditionally, natural compounds are deemed promising agents for cancer treatment. Cantharidin (CTD)-a terpenoid isolated from blister beetles-has been used extensively in traditional Chinese medicines for healing various maladies and cancer. CTD has been proven to be protein phosphatase 2A (PP2A) and heat shock transcription factor 1 (HSF-1) inhibitor, which can be potential targets for its anticancer activity. Albeit, it harbors some toxicities, its immense anticancer potential cannot be overlooked, as the cancer-specific delivery of CTD could help to rescue its lethal effects. Furthermore, several derivatives have been designed to weaken its toxicity. In light of extensive research, the antitumor activity of CTD is evident in both in vitro as well as in vivo cancer models. CTD has also proven efficacious in combination with chemotherapy and radiotherapy and it can also target some drug-resistant cancer cells. This mini-review endeavors to interpret and summarize recent information about CTD anticancer potential and underlying molecular mechanisms. The pertinent anticancer strength of CTD could be employed to develop an effective anticarcinogenic drug.

Contributions to the Knowledge of Nemognathinae (Coleoptera: Meloidae) from China.

Despite being the most widespread blister beetle subfamily, Nemognathinae is unfairly understudied in China. In this study, a new genus and species, Sinostenoria yangi Pan, from northern China is described and illustrated. The antennae, elytra, hind wings, and claws of the new genus form a truly unique set of characteristics never observed in other genera of Nemognathini Laporte de Castelnau, 1840. Three species from China are newly recorded and illustrated: Megatrachelus sibiricus (Tauscher, 1812), Zonitomorpha dollei (Fairmaire 1889), and Stenodera djakonovi Aksentjev, 1978. The genus Oreomeloe Tan, 1981, is transferred from the tribe Meloini Gyllenhal, 1910, to Nemognathini based on an examination of the types. Aiming to test the morphology-based placement of the new genus, we conducted molecular phylogenetic analyses using two mitochondrial (COI, 16S) and three nuclear markers (28S, CAD, ITS2). The results confirm our tribal assignment of the new genus and support a clade that consists of Sinostenoriagen. n., Longizonitis Pan and Bologna, 2018, Stenoria cf. grandiceps, and Ctenopus cf. persicus.

Blister beetle dermatitis caused by cantharidin in South Sudan in Op TRENTON: a case series.

Cantharidin-producing blister beetles are found worldwide. The pathognomonic feature of their toxin is a blistering dermatitis that presents an environmental health hazard. Cutaneous exposure to cantharidin can produce blistering dermatitis, most commonly seen on exposed skin, in the Bentiu region of South Sudan. This should be treated with appropriate cleaning, debridement and regular dressing changes to cope with extensive initial exudate. The best dressing combinations found were initial treatment with povidone-iodine and hydrocolloid, followed by hydrocolloid only. Hydrocolloid dressings were found to be the most effective at staying in place with South Sudan's high humidity. Prevention strategies should include covering exposed skin, wearing wide-brimmed hats, neck scarves and enclosed footwear, and avoidance of working under white light. Medical personnel should engage with the chain of command to include appropriate force protection education within the arrivals brief.

Cytogenetic Analysis, Heterochromatin Characterization and Location of the rDNA Genes of Hycleus scutellatus (Coleoptera, Meloidae); A Species with an Unexpected High Number of rDNA Clusters.

Meloidae are commonly known as blister beetles, so called for the secretion of cantharidin, a toxic substance that causes irritation and blistering. There has been a recent increase in the interest of the cantharidin anticancer potential of this insect group. Cytogenetic and molecular data in this group are scarce. In this study, we performed a karyotype analysis of Hycleus scutellatus, an endemic species of the Iberian Peninsula. We determined its chromosome number, 2n = 20, as well as the presence of the X and Y sex chromosomes. In addition to a karyotype analysis, we carried out DAPI staining. By fluorescence in situ hybridization we mapped the rDNA clusters on 12 different chromosomes. Compared to others, this species shows an unusually high number of chromosomes carrying rDNA. This is one of the highest numbers of rDNA sites found in the Polyphaga suborder (Coleoptera). Additionally, we isolated a satellite DNA family (Hyscu-H), which was located within the pericentromeric regions of all chromosomes, including the sex chromosomes. The results suggest that Hyscu-H is likely to be one of the most abundant satellite DNA repeats in H. scutellatus.

Mylabris (Mylabris) snizeki sp. nov. from Jordan, with a key to the Jordanian species of the nominotypical subgenus (Coleoptera: Meloidae).

Mylabris (Mylabris) snizeki sp. nov. from Jordan is described and an identification key to Jordanian species of the nominotypical subgenus is provided.

Checklist of Epicauta Dejean from America (Meloidae, Meloinae, Epicautini).

This paper presents a comprehensive list of American Epicauta. Two hundred and sixty-four named species were recorded in this checklist based primarily on literature and museum records. Seventy-two species were included in the subgenus Macrobasis and 192 species into the nominotypic subgenus. Nomenclatural modifications are provided for E.langei Borchmann and E.nigripes Borchmann, both junior synonyms of E.pilme (Molina); E.albicincta Borchmann, junior synonym of E.suturalis (Haag-Rutenberg); E.lugubris Denier, junior synonym of E.tristis (Mäklin); and E.wagneri Pict, junior synonym of E.vicina (Haag-Rutenberg). Three hundred and ninety-two new records for South America are provided.

Longizonitis, a new nemognathine genus from the Himalayas (Coleoptera, Meloidae).

The new blister beetle genus Longizonitis Pan and Bologna is described. The genus is referred to the tribe Nemognathini, subfamily Nemognathinae, and its relationships are briefly discussed. It is distributed in southern China (Yunnan, SE Xizang, and probably Fujian) and India (Uttarakhand), in a transitional area between the Palaearctic and Oriental regions. The type species, Longizonitis semirubra (Pic, 1911), comb. n., is re-described and illustrated.

Meloidae of Namibia (Coleoptera): taxonomy and faunistics with biogeographic and ecological notes.

The blister beetle (Coleoptera: Meloidae) fauna of Namibia is studied. The species are arranged within a catalogue containing information on their general distribution, including a list of localities and brief taxonomic remarks. Zoogeographic and ecological analyses were carried out and a photographic appendix, with images and maps of almost all Namibian species, is included. According to a chorological analysis, the Namibian blister beetle fauna appears to be zoogeographically distinct because of the dominance of western southern African elements. The faunistic levels of similarity among distinct areas in Namibia are also analysed. Several of the endemic species are related to the xeric ecosystems referable to the Namib Desert, Succulent Karoo and Nama Karoo biomes, but the highest diversity is related to the ecosystems referable to the Savannah biome. A total of 148 species belonging to 28 genera, eight tribes and three subfamilies (Eleticinae,  Meloinae, Nemognathinae) are recorded from this southern African country. Five new genera (Namibeletica gen. nov., Eleticinae Eleticini; Dilatilydus gen. nov. and Desertilydus gen. nov., Meloinae Lyttini; Paramimesthes gen. nov. and Namylabris gen. nov, Meloinae Mylabrini) and a total of 13 new species are described: 11 new species from Namibia (Psalydolytta gessi sp.nov., Paramimesthes namibicus sp.nov., Namylabris adamantifera sp.nov., Hycleus arlecchinus sp.nov., H. planitiei sp.nov., H. dvoraki sp.nov., H. aridus sp.nov., H. san sp.nov., Nemognatha fluviatilis sp.nov., "Zonitoschema" deserticola sp.nov., Zonitoschema dunalis sp.nov.); a new Namibeletica from the Angolan Namib (N. angolana) and a new Afrolytta Kaszab, 1959 from the S African Namaqualand (A. namaqua), both close to Namibian borders. Fourty-eight species and the genus Apalus Fabricius, 1775 are recorded for the first time from Namibia, and a few other species from South Africa (1), Zambia (1), Botswana (1) and Congo (1). The following new synonymies are proposed: Lytta pleuralis var. inpleuralis Pic, 1911 = Lydomorphus (Lydomorphus) thoracicus (Erichson, 1843), syn. nov.; Lytta benguellana Pic, 1911 = Prionotolytta melanura (Erichson, 1843), syn. nov.; Actenodia amoena ssp.anthicoides Kaszab, 1955b = Hycleus amoenus (Marseul, 1872), syn. nov.; Decapotoma csikii Kaszab, 1953 = Hycleus benguellanus (Marseul, 1879), syn. nov.; Nemognatha capensis Péringuey, 1909 = Nemognatha peringueyi Fairmaire, 1883, syn. nov. Several new combinations in the genus Hycleus Latreille, 1817 are also established.

Taxonomic and faunistic study of four almost unknown Brazilian Meloidae (Coleoptera)

ABSTRACT The Brazilian fauna of Meloidae is poorly studied, even though it includes more than 160 species. In this paper, we aimed at widening the knowledge on four species of blister beetles from this country. Specifically, we defined the uncertain range of Tetraonyx angulicollis, as extended in south-eastern Brazil rather than in Mexico, and implemented the description of the species with figures. We studied the taxonomy and distribution of three almost unknown species of Nemognatha from Brazil, São Paulo State, providing descriptions and figures of sexual characters and colour variability of N. beauregardi, to which is probably referable as a junior synonym of N. plaumanni, of N. rufoscutellaris and of N. cfr. gounellei. Moreover, we assigned these three species to the subgenus Pauronemognatha, recently recorded from South America.

A new Eastern Asian Hycleus and key to the Chinese species of the phaleratus group (Coleoptera, Meloidae, Mylabrini).

A new species of Hycleus belonging to the phaleratus group, and close to Hycleusphaleratus, is described. The new species, Hycleusmarcipoli, is distributed in China (Gansu and Taiwan), Laos, and northern Thailand. A key to the Chinese species of this group is presented.

Cantharidin-based small molecules as potential therapeutic agents.

Chemical and pharmacological information on cantharidin-based small molecules was analyzed. The review summarizes new facts about blister beetles' metabolites for the period 2006-2012. General synthetic approaches to cantharidin-based small molecules as well as their chemical transformations and biological activities related to cantharidin, norcantharidin, cantharidimide, and norcantharimide analogs, especially their inhibitory activity of phosphoprotein phosphatases in cancer treatment, were discussed in this mini review, which could help to design new small molecule modulators for other biological models.