Structural basis of assembly and torque transmission of the bacterial flagellar motor.

The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key determinant of pathogenicity. The detailed structure of the flagellar motor remains unknown. Here we present an atomic-resolution cryoelectron microscopy (cryo-EM) structure of the bacterial flagellar motor complexed with the hook, consisting of 175 subunits with a molecular mass of approximately 6.3 MDa. The structure reveals that 10 peptides protruding from the MS ring with the FlgB and FliE subunits mediate torque transmission from the MS ring to the rod and overcome the symmetry mismatch between the rotational and helical structures in the motor. The LP ring contacts the distal rod and applies electrostatic forces to support its rotation and torque transmission to the hook. This work provides detailed molecular insights into the structure, assembly, and torque transmission mechanisms of the flagellar motor.

Design and Control of Multi-Plate MR Clutch Featuring Friction and Magnetic Field Control Modes.

A magnetorheological (MR) multi-plate clutch was proposed with both mechanical friction mode and magnetic field control modes. The magnetic field control mode was based on an MR fluid coupler that changed its viscous properties according to the density of an applied magnetic field. This mode was used in the early stage of clutch operation to reduce the impact of friction between the disc and plate, and eliminate to the extent possible the difference in their relative speeds when contacting each other in later stages. Once the rotational speed difference between the disc and plate was reduced, the clutch was operated in mechanical friction mode by compressing the friction surfaces together. A torque modeling equation was then derived for each mode based on the Bingham model of the MR fluid, and the transmission torque of the proposed multi-plate clutch was derived using these equations as well as magnetic field analysis results obtained using ANSYS Maxwell. A multi-plate MR clutch was then fabricated, and its torque transmission characteristics were evaluated in the magnetic field control and mechanical friction modes. The results confirmed that the model-based torque calculations were consistent with the observed transmission torque. Finally, control algorithms for mechanical friction only and mixed mechanical friction/magnetic field control torque tracking of the proposed MR multi-plate clutch were designed, and their performances were evaluated when applying unit step command, half-sine-wave command, and rotational speed changes. The results indicated that the torque tracking control was performed smoothly, demonstrating the advantages of the proposed clutch.

Wireless power transmission in endoscopy capsules.

The operating life of endoscopy capsules is limited by the batteries needed for use. The capacity of corresponding batteries, however, is too short to cover the total period of gastrointestinal transit. To overcome this limitation, a novel kind of wireless power transmission was used. It utilizes a permanent magnetic cylinder located outside the patient's body and is turned by an electric motor, thereby generating a rotating magnetic field. This field in turn causes the motor, by a permanent magnetic sphere, located in a liquid bearing inside the endoscopy capsule, to rotate synchronously. The sphere induces an alternating voltage in coils inside the capsule and thus provides the necessary power. This arrangement was able to transmit power of up to 170 mW. This value is clearly higher than the minimum power of 100 mW needed to operate the electronics of the endoscopy capsules that are actually in use. The volume of both the sphere and the induction coil is smaller than the batteries that are actually integrated in the capsules. By this means, the operating time may be prolonged, in principle up to arbitrary values.